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The map label for this gene is mleN [H]

Identifier: 119776067

GI number: 119776067

Start: 3487928

End: 3489265

Strand: Direct

Name: mleN [H]

Synonym: Sama_2935

Alternate gene names: 119776067

Gene position: 3487928-3489265 (Clockwise)

Preceding gene: 119776066

Following gene: 119776068

Centisome position: 81.0

GC content: 54.56

Gene sequence:

>1338_bases
TTGAATCAATCCCCAGACACTCAGGGCAACGCAAGTGCTGCTTCATTCGTTGCCCTGCTGCCACTGTTTCTGTTTTTGGC
GCTTTTCATTGGCGCAGGTGTGTACTTCACCAGTCAGGGCGTGGACTTTGCCTTCTATCAGTTGCCCAGTGTAGTGGCTA
TTCTGCCTGCCATAGTGCTGGCGCTGCTGCTGTCAAAGCAAAAGCTAAATCAGGCCATAGATACTTTTATCGGCGGTATT
GGCCACAGCAATATCATTGCCATGTGCTTGATTTACTTGCTGGCGGGCGCCTTCGCCTCGGTAGCCAAGGCCACCGGAGG
CGTGGATGCCACCGTCGCCCTTGGCCTGTCACTTATTCCCTCTGATCTGCTGCTGCCGGGCTTTTTTGTGATTGCCGCCT
TTATTGCCACTGCCATGGGTACTTCCATGGGCACCATAGCTGCGGTTGCGCCCATTGCTTTGGGCGTGTCCCAGGAAGCC
GATATTTCGCTGCCGCTGATGGCAGGTGCCATCATTTCCGGCGCCCTATTTGGTGATAACCTGTCCATCATTTCCGACAC
CACCATTGCTGCAACCCGCACCCAGGGCTGCAACATGAAAGACAAATTCCGTGAGAACCTGATTTTCGCATTGCCAGCGT
CACTCATCACCCTGCTGGCCTTTACCCTGGCGGGTCAGGGCGAAGCCAATGTGGCGCCTCAGTCGGTGGACTTTGTTAAG
GTGCTGCCTTATCTGTGCATTCTGTTTTTGGCGGTCGCCGGGCTGAACGTGTTTGTGGTGCTGGGTATTGGTATTCTGCT
GGCCGGTTTGGTGGGCATGTTCACCACCGACTACAGCTGGGTCAGCTTCAGCAAAGATATTTACGCCGGTTTTGGCAACA
TGCAGGAAATCTTTATCCTGTCCATGTTAGTGGGTGGTCTGGCTGCGCTGATGCAGCAGCAGGGTGGCCTTGCCTTTGTC
AGCCGCTTTATCGAAGGACTGATCGCCCGTTTTTCCAAGGCAAAGGGCGAAGCGTCCTGCCGCGCCGCTGAATTGGGTAT
GGCAGGCATAGTGTCCCTGACCAACGCCTGTGTGGCCAACAACACAGTATCCATTGTGATAAGCGGTGATATCGCCCGGG
ATCTCGCCCAGAAGCATGGCGTCAGCGCCAAGCGCAGCGCCAGCGTGCTCGATATATTTTCCTGTATTGTTCAAGGACTT
ATACCGTATGGCGCCCAGGCACTTTTGATTGGGGCAACCTTTGCCATTACCCCCCTGGAGGCAGTATCCTATGCATGGTA
CTGCATGATATTGGCGCTGGTAGCCGTTCTGATCGTGACATTCAGACAACGTCACTGA

Upstream 100 bases:

>100_bases
AAGTTGTCGGGAGATGAAGCCGTAACGACACAAATATTCAGCGAAATCGCGGTGATGCAGGAAGAACCCGCCATTTCGCC
CCCGATAAAGAGAGATAACC

Downstream 100 bases:

>100_bases
GGATCAGGAGACTGCCACCCGGGAAAACCTATGACAATCAAAGACAGAAACGACTATGGACAATTTGATCCCCACCGGCT
GGTGTTTCATATGTCCATTG

Product: Na+/H+ antiporter family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MNQSPDTQGNASAASFVALLPLFLFLALFIGAGVYFTSQGVDFAFYQLPSVVAILPAIVLALLLSKQKLNQAIDTFIGGI
GHSNIIAMCLIYLLAGAFASVAKATGGVDATVALGLSLIPSDLLLPGFFVIAAFIATAMGTSMGTIAAVAPIALGVSQEA
DISLPLMAGAIISGALFGDNLSIISDTTIAATRTQGCNMKDKFRENLIFALPASLITLLAFTLAGQGEANVAPQSVDFVK
VLPYLCILFLAVAGLNVFVVLGIGILLAGLVGMFTTDYSWVSFSKDIYAGFGNMQEIFILSMLVGGLAALMQQQGGLAFV
SRFIEGLIARFSKAKGEASCRAAELGMAGIVSLTNACVANNTVSIVISGDIARDLAQKHGVSAKRSASVLDIFSCIVQGL
IPYGAQALLIGATFAITPLEAVSYAWYCMILALVAVLIVTFRQRH

Sequences:

>Translated_445_residues
MNQSPDTQGNASAASFVALLPLFLFLALFIGAGVYFTSQGVDFAFYQLPSVVAILPAIVLALLLSKQKLNQAIDTFIGGI
GHSNIIAMCLIYLLAGAFASVAKATGGVDATVALGLSLIPSDLLLPGFFVIAAFIATAMGTSMGTIAAVAPIALGVSQEA
DISLPLMAGAIISGALFGDNLSIISDTTIAATRTQGCNMKDKFRENLIFALPASLITLLAFTLAGQGEANVAPQSVDFVK
VLPYLCILFLAVAGLNVFVVLGIGILLAGLVGMFTTDYSWVSFSKDIYAGFGNMQEIFILSMLVGGLAALMQQQGGLAFV
SRFIEGLIARFSKAKGEASCRAAELGMAGIVSLTNACVANNTVSIVISGDIARDLAQKHGVSAKRSASVLDIFSCIVQGL
IPYGAQALLIGATFAITPLEAVSYAWYCMILALVAVLIVTFRQRH
>Mature_445_residues
MNQSPDTQGNASAASFVALLPLFLFLALFIGAGVYFTSQGVDFAFYQLPSVVAILPAIVLALLLSKQKLNQAIDTFIGGI
GHSNIIAMCLIYLLAGAFASVAKATGGVDATVALGLSLIPSDLLLPGFFVIAAFIATAMGTSMGTIAAVAPIALGVSQEA
DISLPLMAGAIISGALFGDNLSIISDTTIAATRTQGCNMKDKFRENLIFALPASLITLLAFTLAGQGEANVAPQSVDFVK
VLPYLCILFLAVAGLNVFVVLGIGILLAGLVGMFTTDYSWVSFSKDIYAGFGNMQEIFILSMLVGGLAALMQQQGGLAFV
SRFIEGLIARFSKAKGEASCRAAELGMAGIVSLTNACVANNTVSIVISGDIARDLAQKHGVSAKRSASVLDIFSCIVQGL
IPYGAQALLIGATFAITPLEAVSYAWYCMILALVAVLIVTFRQRH

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 46275; Mature: 46275

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQSPDTQGNASAASFVALLPLFLFLALFIGAGVYFTSQGVDFAFYQLPSVVAILPAIVL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHH
ALLLSKQKLNQAIDTFIGGIGHSNIIAMCLIYLLAGAFASVAKATGGVDATVALGLSLIP
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
SDLLLPGFFVIAAFIATAMGTSMGTIAAVAPIALGVSQEADISLPLMAGAIISGALFGDN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
LSIISDTTIAATRTQGCNMKDKFRENLIFALPASLITLLAFTLAGQGEANVAPQSVDFVK
CCEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
VLPYLCILFLAVAGLNVFVVLGIGILLAGLVGMFTTDYSWVSFSKDIYAGFGNMQEIFIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHCCCCHHHHHHH
SMLVGGLAALMQQQGGLAFVSRFIEGLIARFSKAKGEASCRAAELGMAGIVSLTNACVAN
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
NTVSIVISGDIARDLAQKHGVSAKRSASVLDIFSCIVQGLIPYGAQALLIGATFAITPLE
CCEEEEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHH
AVSYAWYCMILALVAVLIVTFRQRH
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNQSPDTQGNASAASFVALLPLFLFLALFIGAGVYFTSQGVDFAFYQLPSVVAILPAIVL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHH
ALLLSKQKLNQAIDTFIGGIGHSNIIAMCLIYLLAGAFASVAKATGGVDATVALGLSLIP
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
SDLLLPGFFVIAAFIATAMGTSMGTIAAVAPIALGVSQEADISLPLMAGAIISGALFGDN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
LSIISDTTIAATRTQGCNMKDKFRENLIFALPASLITLLAFTLAGQGEANVAPQSVDFVK
CCEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
VLPYLCILFLAVAGLNVFVVLGIGILLAGLVGMFTTDYSWVSFSKDIYAGFGNMQEIFIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHCCCCHHHHHHH
SMLVGGLAALMQQQGGLAFVSRFIEGLIARFSKAKGEASCRAAELGMAGIVSLTNACVAN
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
NTVSIVISGDIARDLAQKHGVSAKRSASVLDIFSCIVQGLIPYGAQALLIGATFAITPLE
CCEEEEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHH
AVSYAWYCMILALVAVLIVTFRQRH
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]