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The map label for this gene is ispB [H]

Identifier: 119775962

GI number: 119775962

Start: 3367372

End: 3368373

Strand: Direct

Name: ispB [H]

Synonym: Sama_2830

Alternate gene names: 119775962

Gene position: 3367372-3368373 (Clockwise)

Preceding gene: 119775954

Following gene: 119775964

Centisome position: 78.2

GC content: 54.49

Gene sequence:

>1002_bases
ATGCCCAGTCCTGACAATCAGAGTCTATCTATGGATTTGAATGCCATTCGACAGCTGGCCGACGCCGATATGCAGGCAGT
TAATCAGCTGATCTATAAACAACTGGAATCCGATGTTGCCCTCATCAACCAGCTGGGCTTTTACATTATTAATGGAGGCG
GCAAACGTCTGCGCCCTCTGTTATCGGTGTTGGCCGCCCGCGCCGTTAACTATAATGGTGAAGGTCATCTGAAGCTCGCC
GCCATTATCGAGTTTATCCATACCGCTTCGCTGCTGCACGACGACGTGGTGGATGAGTCTACCTTGCGCCGGGGCCGCGA
AACCGCCAATGCCCTCTTTGGCAACAGCGCCAGTGTATTGGTGGGTGACTTCCTTTATACCCGCTCATTCCAGATGATGA
CTGAGCTTGGCAGTATGAAGGTGCTGCAGGTGCTTGCCGATGCCACCAACGTGCTGGCCGAAGGGGAAGTGCTGCAGTTG
ATGAACTGCAACGACCCCAATACCACCGAAGAAAGCTACATGCGGGTTATCTACTGCAAAACCGCCAAGCTGTTTGAAGC
GGCTACCCGTCTTGCCGCCGTGCTGGCAGGCAGCGATGAAAAGGTAGAAGAAGCCCTTGCCGACTATGGAAAGTACCTCG
GCACGGCCTTCCAGCTGATTGACGACTTGCTGGACTACACCAGTGACGCCGAAGAGCTGGGCAAAAACATCGGTGACGAC
CTTGCCGAAGGTAAGCCTACCCTGCCACTGATTTACGCTATCGGCCATGGTAACGAGCGCGAGCAAGGCCTCATTCGTAA
TGCCATTGAAAAAGCCGATGGCACAGGTGCCATTGAAGAAATCCTCACGGCCCTGCATCGCTGTGGTGCGCTTGACTACA
CCCGCAGACGCGCTGAAGAAGAAGCAGACAAAGCCATTGCGGCATTAAGCGTACTGCCGGAAGGTGACTTTAAGACAGCG
CTTGAATCGCTGGCCCGAATCGCCGTATCCCGCAGCAACTGA

Upstream 100 bases:

>100_bases
CGCATAACTCCCACATTAACCGGGCAACTGCTGCCATCGTCATTATTTTTAGGTAAGATCGCGACTATTATAACACTTAT
GCCTAAATCAGGGCTTAGTT

Downstream 100 bases:

>100_bases
TAGCTTGCCAGACCCAATAAAAAACCTCGCATCGGCGAGGTTTTTTATTGGGCTGTGTTTATCCACACAACGAGCGTTAC
AGGCGCCAGTCGATGGGCTC

Product: trans-hexaprenyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MPSPDNQSLSMDLNAIRQLADADMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLSVLAARAVNYNGEGHLKLA
AIIEFIHTASLLHDDVVDESTLRRGRETANALFGNSASVLVGDFLYTRSFQMMTELGSMKVLQVLADATNVLAEGEVLQL
MNCNDPNTTEESYMRVIYCKTAKLFEAATRLAAVLAGSDEKVEEALADYGKYLGTAFQLIDDLLDYTSDAEELGKNIGDD
LAEGKPTLPLIYAIGHGNEREQGLIRNAIEKADGTGAIEEILTALHRCGALDYTRRRAEEEADKAIAALSVLPEGDFKTA
LESLARIAVSRSN

Sequences:

>Translated_333_residues
MPSPDNQSLSMDLNAIRQLADADMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLSVLAARAVNYNGEGHLKLA
AIIEFIHTASLLHDDVVDESTLRRGRETANALFGNSASVLVGDFLYTRSFQMMTELGSMKVLQVLADATNVLAEGEVLQL
MNCNDPNTTEESYMRVIYCKTAKLFEAATRLAAVLAGSDEKVEEALADYGKYLGTAFQLIDDLLDYTSDAEELGKNIGDD
LAEGKPTLPLIYAIGHGNEREQGLIRNAIEKADGTGAIEEILTALHRCGALDYTRRRAEEEADKAIAALSVLPEGDFKTA
LESLARIAVSRSN
>Mature_332_residues
PSPDNQSLSMDLNAIRQLADADMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLSVLAARAVNYNGEGHLKLAA
IIEFIHTASLLHDDVVDESTLRRGRETANALFGNSASVLVGDFLYTRSFQMMTELGSMKVLQVLADATNVLAEGEVLQLM
NCNDPNTTEESYMRVIYCKTAKLFEAATRLAAVLAGSDEKVEEALADYGKYLGTAFQLIDDLLDYTSDAEELGKNIGDDL
AEGKPTLPLIYAIGHGNEREQGLIRNAIEKADGTGAIEEILTALHRCGALDYTRRRAEEEADKAIAALSVLPEGDFKTAL
ESLARIAVSRSN

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=321, Percent_Identity=31.4641744548287, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI4758430, Length=273, Percent_Identity=27.1062271062271, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI83700220, Length=273, Percent_Identity=27.1062271062271, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=64.1744548286604, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1786623, Length=229, Percent_Identity=31.4410480349345, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17505681, Length=322, Percent_Identity=35.0931677018634, Blast_Score=173, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6319475, Length=299, Percent_Identity=33.7792642140468, Blast_Score=151, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6325188, Length=238, Percent_Identity=25.6302521008403, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24651612, Length=298, Percent_Identity=32.8859060402685, Blast_Score=168, Evalue=5e-42,
Organism=Drosophila melanogaster, GI281365769, Length=290, Percent_Identity=25.1724137931034, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24660002, Length=290, Percent_Identity=25.1724137931034, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36243; Mature: 36112

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPDNQSLSMDLNAIRQLADADMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPL
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHH
LSVLAARAVNYNGEGHLKLAAIIEFIHTASLLHDDVVDESTLRRGRETANALFGNSASVL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHH
VGDFLYTRSFQMMTELGSMKVLQVLADATNVLAEGEVLQLMNCNDPNTTEESYMRVIYCK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHH
TAKLFEAATRLAAVLAGSDEKVEEALADYGKYLGTAFQLIDDLLDYTSDAEELGKNIGDD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHH
LAEGKPTLPLIYAIGHGNEREQGLIRNAIEKADGTGAIEEILTALHRCGALDYTRRRAEE
HHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
EADKAIAALSVLPEGDFKTALESLARIAVSRSN
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSPDNQSLSMDLNAIRQLADADMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPL
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHH
LSVLAARAVNYNGEGHLKLAAIIEFIHTASLLHDDVVDESTLRRGRETANALFGNSASVL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHH
VGDFLYTRSFQMMTELGSMKVLQVLADATNVLAEGEVLQLMNCNDPNTTEESYMRVIYCK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHH
TAKLFEAATRLAAVLAGSDEKVEEALADYGKYLGTAFQLIDDLLDYTSDAEELGKNIGDD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHH
LAEGKPTLPLIYAIGHGNEREQGLIRNAIEKADGTGAIEEILTALHRCGALDYTRRRAEE
HHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
EADKAIAALSVLPEGDFKTALESLARIAVSRSN
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]