Click here to switch to the map view.

The map label for this gene is metR [H]

Identifier: 119775760

GI number: 119775760

Start: 3147751

End: 3148650

Strand: Direct

Name: metR [H]

Synonym: Sama_2628

Alternate gene names: 119775760

Gene position: 3147751-3148650 (Clockwise)

Preceding gene: 119775757

Following gene: 119775766

Centisome position: 73.1

GC content: 56.33

Gene sequence:

>900_bases
ATGATAGAGCTAAGACACCTTCGAACCCTGATGGCACTGAAAGAGAGTGGCAGCCTGGCGGCCGCGGCCAAAAAGCGCTT
TGTCACCCAGTCTGCTTTGTCTCATCAGATTAAAGAGCTGGAAACCCGCATCAATTCCCCCGTGTTCGTGAGAAAGAGCA
AGCCCCTGTCTTTTACTCAGGAAGGCGCCCGACTGCTGAATCTGGCCGAAGAAATTCTGCCGCGAGTGATGGAAACCGAG
TTTGTATTGAAGAAAGGTCTGGAGACCGACGCCCATTTGCTCAAGGTCGGTATTGAGTGTCACAGCTGCTTTCGCTGGCT
GATGCCGGTTATAGAGACTTTCAGGGCTGAATATCCGGATGCGGACTTGGACTTATCGAGCCGTCACCTGTTCGACTCGC
TGAACGCACTGGAGTCCGGTGAGCTGGACATTGTACTGACATCCGATCCCGTCCCCGGGCATCAGCTGGCTTATCAGCAC
CTGTTCGACTTTGAAGTTCGCCTGGTGGTGGCCAAAGAGCATCCGCTGGCGAGCAAGAGCCTGGTGCTGCCAAAGGATCT
GGCGGGCTTGCCCATTATAAGCTACCCCGTACCCTTGGAACGCCTTGACCTGTTCCGCTTGTTTTTAGAGCCAGCCGGTA
TTGAGCCCGGTGTGCAAAAGAGCTGCGATCTGACCTCGGTCCTGTTTCAACGCATCGCCTGTCAGGATGGTATAGCCGCC
CTGCCCAGTTGGTCCATCAGCGAGGCCCAGGGCCTTAGCCTCACCAGTTTGCGCCTCGGCGCTGAGGGCGTAAAACGCCC
ACTGTTTGGCGCCTATCGCCGTGGCAGTGCCAACTCCCGCCAACTGCAACGGTACCTTGAACTGGTGGCAGCAGAAGGTC
TGGCTCGCCAATCACAGTAA

Upstream 100 bases:

>100_bases
AATAGGCGTCTGGACGTCTAGAAGTTTATTGTGGTAGTCTTCTGGCAAGCAAACGAATTGTGTTCACGGGCAACATGAAC
AAACTTCATGTGGAGTAGAG

Downstream 100 bases:

>100_bases
GCTCGTCTTATACACGGCATGACTCAACAATCGCCCATATAAAAAACGCCTGCAACTGCAGGCGTTTTTTATATCCAACT
CAGTAAATTACAGTGGATTG

Product: transcriptional activator protein MetR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MIELRHLRTLMALKESGSLAAAAKKRFVTQSALSHQIKELETRINSPVFVRKSKPLSFTQEGARLLNLAEEILPRVMETE
FVLKKGLETDAHLLKVGIECHSCFRWLMPVIETFRAEYPDADLDLSSRHLFDSLNALESGELDIVLTSDPVPGHQLAYQH
LFDFEVRLVVAKEHPLASKSLVLPKDLAGLPIISYPVPLERLDLFRLFLEPAGIEPGVQKSCDLTSVLFQRIACQDGIAA
LPSWSISEAQGLSLTSLRLGAEGVKRPLFGAYRRGSANSRQLQRYLELVAAEGLARQSQ

Sequences:

>Translated_299_residues
MIELRHLRTLMALKESGSLAAAAKKRFVTQSALSHQIKELETRINSPVFVRKSKPLSFTQEGARLLNLAEEILPRVMETE
FVLKKGLETDAHLLKVGIECHSCFRWLMPVIETFRAEYPDADLDLSSRHLFDSLNALESGELDIVLTSDPVPGHQLAYQH
LFDFEVRLVVAKEHPLASKSLVLPKDLAGLPIISYPVPLERLDLFRLFLEPAGIEPGVQKSCDLTSVLFQRIACQDGIAA
LPSWSISEAQGLSLTSLRLGAEGVKRPLFGAYRRGSANSRQLQRYLELVAAEGLARQSQ
>Mature_299_residues
MIELRHLRTLMALKESGSLAAAAKKRFVTQSALSHQIKELETRINSPVFVRKSKPLSFTQEGARLLNLAEEILPRVMETE
FVLKKGLETDAHLLKVGIECHSCFRWLMPVIETFRAEYPDADLDLSSRHLFDSLNALESGELDIVLTSDPVPGHQLAYQH
LFDFEVRLVVAKEHPLASKSLVLPKDLAGLPIISYPVPLERLDLFRLFLEPAGIEPGVQKSCDLTSVLFQRIACQDGIAA
LPSWSISEAQGLSLTSLRLGAEGVKRPLFGAYRRGSANSRQLQRYLELVAAEGLARQSQ

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=277, Percent_Identity=47.6534296028881, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI1788887, Length=193, Percent_Identity=29.5336787564767, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87082024, Length=176, Percent_Identity=32.9545454545455, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790208, Length=195, Percent_Identity=26.1538461538462, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI145693105, Length=191, Percent_Identity=25.6544502617801, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI87081904, Length=199, Percent_Identity=30.1507537688442, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI157672245, Length=253, Percent_Identity=24.901185770751, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787879, Length=195, Percent_Identity=26.1538461538462, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788296, Length=187, Percent_Identity=28.8770053475936, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787601, Length=165, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1786401, Length=169, Percent_Identity=25.4437869822485, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790399, Length=189, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33292; Mature: 33292

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELRHLRTLMALKESGSLAAAAKKRFVTQSALSHQIKELETRINSPVFVRKSKPLSFTQ
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHH
EGARLLNLAEEILPRVMETEFVLKKGLETDAHLLKVGIECHSCFRWLMPVIETFRAEYPD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ADLDLSSRHLFDSLNALESGELDIVLTSDPVPGHQLAYQHLFDFEVRLVVAKEHPLASKS
CCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCCCCCC
LVLPKDLAGLPIISYPVPLERLDLFRLFLEPAGIEPGVQKSCDLTSVLFQRIACQDGIAA
CCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
LPSWSISEAQGLSLTSLRLGAEGVKRPLFGAYRRGSANSRQLQRYLELVAAEGLARQSQ
CCCCCCCCCCCCCHHHHHHCHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIELRHLRTLMALKESGSLAAAAKKRFVTQSALSHQIKELETRINSPVFVRKSKPLSFTQ
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHH
EGARLLNLAEEILPRVMETEFVLKKGLETDAHLLKVGIECHSCFRWLMPVIETFRAEYPD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ADLDLSSRHLFDSLNALESGELDIVLTSDPVPGHQLAYQHLFDFEVRLVVAKEHPLASKS
CCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCCCCCC
LVLPKDLAGLPIISYPVPLERLDLFRLFLEPAGIEPGVQKSCDLTSVLFQRIACQDGIAA
CCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
LPSWSISEAQGLSLTSLRLGAEGVKRPLFGAYRRGSANSRQLQRYLELVAAEGLARQSQ
CCCCCCCCCCCCCHHHHHHCHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]