Click here to switch to the map view.

The map label for this gene is rlmH

Identifier: 119775719

GI number: 119775719

Start: 3094869

End: 3095339

Strand: Direct

Name: rlmH

Synonym: Sama_2587

Alternate gene names: 119775719

Gene position: 3094869-3095339 (Clockwise)

Preceding gene: 119775718

Following gene: 119775720

Centisome position: 71.87

GC content: 58.17

Gene sequence:

>471_bases
ATGAAACTGCAACTCATCGCCGTGGGCACCAAGATGCCCGATTGGGTTACCCGCGGATTTGAAGAATACCAGCGCCGCTT
CCCCCGTGATATGGCGCTGGAGCTGGTCGAAATTCCCGCTGGCAAGCGCGGCAAAAATGCCGATATCGCCCGCATCCTGC
AAAAGGAGGGTGAACAAATGCTTGCAGCAATTCCCAAGGGTAATCATATTGTTACCCTGGATCTTCCCGGCAAGAACTGG
ACGACTCCCGAACTTGCGAGTGCCCTGTCCAAATGGCAGCTCGATGGCCGGGATGTCAGCCTCCTGATTGGTGGCCCCGA
AGGCCTCGCCCCGGCATGCAAACAGGCCGCGGCCCAGAGCTGGTGTTTGTCGGCGCTGACTCTGCCACATCCCCTGGTGA
GGGTGGTCGTGGCGGAAAGCCTCTACCGGGCCTGGAGCATTAACAACAACCATCCTTATCACAGGGAATAG

Upstream 100 bases:

>100_bases
AATGGGTTCTGGTGGATTTGGGTGAAGTGATTCTGCACGTGATGCAGGAACAAACCCGCGACTTTTATCAGCTCGAAAAG
CTCTGGCAAGAACCCGCCTG

Downstream 100 bases:

>100_bases
TGTGCCCTTGTCAGGCCGCTGATTGCACCGGAGACGTTGAGTGACACCAAGAAAACGGGTAGCCATGCATGACCACGCCG
CTGAGGCAGCATTGTTCAAG

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKLQLIAVGTKMPDWVTRGFEEYQRRFPRDMALELVEIPAGKRGKNADIARILQKEGEQMLAAIPKGNHIVTLDLPGKNW
TTPELASALSKWQLDGRDVSLLIGGPEGLAPACKQAAAQSWCLSALTLPHPLVRVVVAESLYRAWSINNNHPYHRE

Sequences:

>Translated_156_residues
MKLQLIAVGTKMPDWVTRGFEEYQRRFPRDMALELVEIPAGKRGKNADIARILQKEGEQMLAAIPKGNHIVTLDLPGKNW
TTPELASALSKWQLDGRDVSLLIGGPEGLAPACKQAAAQSWCLSALTLPHPLVRVVVAESLYRAWSINNNHPYHRE
>Mature_156_residues
MKLQLIAVGTKMPDWVTRGFEEYQRRFPRDMALELVEIPAGKRGKNADIARILQKEGEQMLAAIPKGNHIVTLDLPGKNW
TTPELASALSKWQLDGRDVSLLIGGPEGLAPACKQAAAQSWCLSALTLPHPLVRVVVAESLYRAWSINNNHPYHRE

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=156, Percent_Identity=80.1282051282051, Blast_Score=258, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_SHEAM (A1S8T3)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928459.1
- ProteinModelPortal:   A1S8T3
- SMR:   A1S8T3
- STRING:   A1S8T3
- GeneID:   4604834
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2587
- NMPDR:   fig|326297.7.peg.2425
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- PhylomeDB:   A1S8T3
- ProtClustDB:   PRK00103
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17359; Mature: 17359

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQLIAVGTKMPDWVTRGFEEYQRRFPRDMALELVEIPAGKRGKNADIARILQKEGEQM
CEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHH
LAAIPKGNHIVTLDLPGKNWTTPELASALSKWQLDGRDVSLLIGGPEGLAPACKQAAAQS
HHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHH
WCLSALTLPHPLVRVVVAESLYRAWSINNNHPYHRE
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKLQLIAVGTKMPDWVTRGFEEYQRRFPRDMALELVEIPAGKRGKNADIARILQKEGEQM
CEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHH
LAAIPKGNHIVTLDLPGKNWTTPELASALSKWQLDGRDVSLLIGGPEGLAPACKQAAAQS
HHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHH
WCLSALTLPHPLVRVVVAESLYRAWSINNNHPYHRE
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA