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The map label for this gene is trmB

Identifier: 119775622

GI number: 119775622

Start: 2991349

End: 2992065

Strand: Reverse

Name: trmB

Synonym: Sama_2489

Alternate gene names: 119775622

Gene position: 2992065-2991349 (Counterclockwise)

Preceding gene: 119775625

Following gene: 119775621

Centisome position: 69.48

GC content: 53.28

Gene sequence:

>717_bases
ATGAGTGAAGTAACCACCGCAGAATTTAACGAGGATGGCGTTTATCTTCGCAAGGTACGCAGCTTTGTTCTGCGTGAAGG
ACGTCTTACTACCGGGCAGGCCAAGGCGCTGGAAAGCTTCTGGCCAACCATGGGTCTCGACTACAGTCCACAGGCGATTA
ATTTTACTGAAGTATTTGGCCGCGAAGCTGATACAGTGCTTGAAATCGGCTTTGGTATGGGTGCCTCTCTGGTTGAAATG
GCTGCCAATGCTCCTGAACTCAACTTTATTGGTGTTGAGGTGCACAAGCCAGGTGTGGGCGCCTGTTTGGGGGAGGCCGG
TAACGCCGGGATCTCCAATCTGCGGGTTTACCACCATGATGCGGTGGAAGTGCTGGAAAACGCCATTCCCGATAGCTCAT
TGGCGCGGGTGCAGCTGTTTTTCCCTGACCCATGGCACAAGAAGCGCCACCACAAGCGCCGCATCGTGCAGCCTGAGTTT
GTTGAGCTTTTACGCCGCAAACTTAAGATTGGTGGTGTGTTCCATATGGCCACCGACTGGGAAAACTACGCCGAGCATAT
GCTTGAGGTGATGAGCGCTGCTCCGGGTTACCAGAATCAGTCTGATACTCAAACTTATGTGCCACGCCCGGACCATCGTC
CACTGACCAAGTTTGAAAACCGTGGTCAGCGCCTCGGTCACGGTGTGTGGGACCTGATGTTTGAGCGTGTTGAATAA

Upstream 100 bases:

>100_bases
AATAACTTTGGATAATGCCCCTTCTTTTTGATTTGTCTGTTTTTAGAAGCCTGCTTTTTGCGCCTGCGCGCAGCACAGTG
GCGGGCCATGCGAGGCGAGA

Downstream 100 bases:

>100_bases
TTTTATCCATCCATGAAAGAGGAAATAACCATGGCCAAAAACCGTAGCCGTCGTCTTCGTAAAAAGCTGCGTGTGGACGA
GTTTCAGGAACTGGGTTTCG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSEVTTAEFNEDGVYLRKVRSFVLREGRLTTGQAKALESFWPTMGLDYSPQAINFTEVFGREADTVLEIGFGMGASLVEM
AANAPELNFIGVEVHKPGVGACLGEAGNAGISNLRVYHHDAVEVLENAIPDSSLARVQLFFPDPWHKKRHHKRRIVQPEF
VELLRRKLKIGGVFHMATDWENYAEHMLEVMSAAPGYQNQSDTQTYVPRPDHRPLTKFENRGQRLGHGVWDLMFERVE

Sequences:

>Translated_238_residues
MSEVTTAEFNEDGVYLRKVRSFVLREGRLTTGQAKALESFWPTMGLDYSPQAINFTEVFGREADTVLEIGFGMGASLVEM
AANAPELNFIGVEVHKPGVGACLGEAGNAGISNLRVYHHDAVEVLENAIPDSSLARVQLFFPDPWHKKRHHKRRIVQPEF
VELLRRKLKIGGVFHMATDWENYAEHMLEVMSAAPGYQNQSDTQTYVPRPDHRPLTKFENRGQRLGHGVWDLMFERVE
>Mature_237_residues
SEVTTAEFNEDGVYLRKVRSFVLREGRLTTGQAKALESFWPTMGLDYSPQAINFTEVFGREADTVLEIGFGMGASLVEMA
ANAPELNFIGVEVHKPGVGACLGEAGNAGISNLRVYHHDAVEVLENAIPDSSLARVQLFFPDPWHKKRHHKRRIVQPEFV
ELLRRKLKIGGVFHMATDWENYAEHMLEVMSAAPGYQNQSDTQTYVPRPDHRPLTKFENRGQRLGHGVWDLMFERVE

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=237, Percent_Identity=65.4008438818565, Blast_Score=332, Evalue=2e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_SHEAM (A1S8I6)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928362.1
- ProteinModelPortal:   A1S8I6
- SMR:   A1S8I6
- STRING:   A1S8I6
- GeneID:   4604737
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2489
- NMPDR:   fig|326297.7.peg.2330
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- PhylomeDB:   A1S8I6
- ProtClustDB:   PRK00121
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 26923; Mature: 26792

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: BINDING 68-68 BINDING 93-93 BINDING 120-120 BINDING 143-143 BINDING 147-147 BINDING 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVTTAEFNEDGVYLRKVRSFVLREGRLTTGQAKALESFWPTMGLDYSPQAINFTEVFG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHC
READTVLEIGFGMGASLVEMAANAPELNFIGVEVHKPGVGACLGEAGNAGISNLRVYHHD
CCCHHHEEECCCCCHHHHHHHCCCCCCEEEEEEEECCCCHHHHCCCCCCCCHHEEEEEHH
AVEVLENAIPDSSLARVQLFFPDPWHKKRHHKRRIVQPEFVELLRRKLKIGGVFHMATDW
HHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEECCCH
ENYAEHMLEVMSAAPGYQNQSDTQTYVPRPDHRPLTKFENRGQRLGHGVWDLMFERVE
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEVTTAEFNEDGVYLRKVRSFVLREGRLTTGQAKALESFWPTMGLDYSPQAINFTEVFG
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHC
READTVLEIGFGMGASLVEMAANAPELNFIGVEVHKPGVGACLGEAGNAGISNLRVYHHD
CCCHHHEEECCCCCHHHHHHHCCCCCCEEEEEEEECCCCHHHHCCCCCCCCHHEEEEEHH
AVEVLENAIPDSSLARVQLFFPDPWHKKRHHKRRIVQPEFVELLRRKLKIGGVFHMATDW
HHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEECCCH
ENYAEHMLEVMSAAPGYQNQSDTQTYVPRPDHRPLTKFENRGQRLGHGVWDLMFERVE
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA