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The map label for this gene is grpE

Identifier: 119775571

GI number: 119775571

Start: 2931494

End: 2932096

Strand: Reverse

Name: grpE

Synonym: Sama_2437

Alternate gene names: 119775571

Gene position: 2932096-2931494 (Counterclockwise)

Preceding gene: 119775578

Following gene: 119775567

Centisome position: 68.09

GC content: 57.88

Gene sequence:

>603_bases
ATGAGCAACGAATCGACCAACCCCCAACAAGAGCCCCTGGAACAGGTACAGGACACCGAAGTCGTCACTGATGAGGCGGC
GCTGGTAGATGAGCTGACCCAGGCAAACTTCCGTATCGAGGAGTTGGAGCAGGCTCTGGCCGCTGCCGAGGCCAAGGTGG
AAGAGCAAAAGGACTCTGTTGTCCGTGCCGCCGCTGAAGTGGATAACATCCGTCGCCGCGCCGCCATGGATGTGGAAAAG
GCCAACAAGTTTGCCCTCGAAAAATTTGCCAACGAGCTGTTGCCGGTCCTGGATAACATGGAGCGCGCACTGGCAGGCAC
CAACGCCGAAGACGAAGCCACCAAGGCCATGTACGAAGGCGTGTCTCTGACCATGAAGACCTTGGTCAATGCCGTGGAAA
AGTTTGGCGTGAAAGTGGTGGACCCTATGGGCCAGCCCTTTAACCCAGAGCAGCACCAGGCCATTGGCATGCAGCCAAAT
CCCGAGGTGCCCGCCAATCATGTGATGGTGGTTCTGCAAAAGGGCTATGAGCTCAATGGCCGTTTGTTGCGTCCTGCCAT
GGTGATGGTATCCCAGGGCGGCGGTGTAGACACCCAGGCCTGA

Upstream 100 bases:

>100_bases
ATCGCGGAAACTGCGCCTTCAAGGCCTTGAAACGCCAGAATTGATCCCCATAATATGCCCTGTGTATGCAGAAACAAAGC
ATTTTTAGCTTGGAGTAGAG

Downstream 100 bases:

>100_bases
TAAGCTGTCAGATAGCAAAAAGGACTGCCTGTGCAGTCCTTTTTTGATTGCGGCTTAATATCAGCGCTCAATAAAGGCGC
GGATTTGCCCCTCAATACCG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MSNESTNPQQEPLEQVQDTEVVTDEAALVDELTQANFRIEELEQALAAAEAKVEEQKDSVVRAAAEVDNIRRRAAMDVEK
ANKFALEKFANELLPVLDNMERALAGTNAEDEATKAMYEGVSLTMKTLVNAVEKFGVKVVDPMGQPFNPEQHQAIGMQPN
PEVPANHVMVVLQKGYELNGRLLRPAMVMVSQGGGVDTQA

Sequences:

>Translated_200_residues
MSNESTNPQQEPLEQVQDTEVVTDEAALVDELTQANFRIEELEQALAAAEAKVEEQKDSVVRAAAEVDNIRRRAAMDVEK
ANKFALEKFANELLPVLDNMERALAGTNAEDEATKAMYEGVSLTMKTLVNAVEKFGVKVVDPMGQPFNPEQHQAIGMQPN
PEVPANHVMVVLQKGYELNGRLLRPAMVMVSQGGGVDTQA
>Mature_199_residues
SNESTNPQQEPLEQVQDTEVVTDEAALVDELTQANFRIEELEQALAAAEAKVEEQKDSVVRAAAEVDNIRRRAAMDVEKA
NKFALEKFANELLPVLDNMERALAGTNAEDEATKAMYEGVSLTMKTLVNAVEKFGVKVVDPMGQPFNPEQHQAIGMQPNP
EVPANHVMVVLQKGYELNGRLLRPAMVMVSQGGGVDTQA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=157, Percent_Identity=32.484076433121, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI40255109, Length=160, Percent_Identity=31.25, Blast_Score=71, Evalue=5e-13,
Organism=Escherichia coli, GI1788967, Length=190, Percent_Identity=40, Blast_Score=144, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17552458, Length=141, Percent_Identity=35.4609929078014, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324806, Length=168, Percent_Identity=35.1190476190476, Blast_Score=112, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24653432, Length=168, Percent_Identity=32.7380952380952, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_SHEAM (A1S8D5)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928311.1
- ProteinModelPortal:   A1S8D5
- SMR:   A1S8D5
- STRING:   A1S8D5
- GeneID:   4604686
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2437
- NMPDR:   fig|326297.7.peg.2281
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NVRRRME
- PhylomeDB:   A1S8D5
- ProtClustDB:   PRK14150
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21890; Mature: 21759

Theoretical pI: Translated: 4.21; Mature: 4.21

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNESTNPQQEPLEQVQDTEVVTDEAALVDELTQANFRIEELEQALAAAEAKVEEQKDSV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
VRAAAEVDNIRRRAAMDVEKANKFALEKFANELLPVLDNMERALAGTNAEDEATKAMYEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VSLTMKTLVNAVEKFGVKVVDPMGQPFNPEQHQAIGMQPNPEVPANHVMVVLQKGYELNG
HHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHCCCCCCCCCCCCHHHEEEHHHCCCCCCC
RLLRPAMVMVSQGGGVDTQA
EEHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SNESTNPQQEPLEQVQDTEVVTDEAALVDELTQANFRIEELEQALAAAEAKVEEQKDSV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
VRAAAEVDNIRRRAAMDVEKANKFALEKFANELLPVLDNMERALAGTNAEDEATKAMYEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VSLTMKTLVNAVEKFGVKVVDPMGQPFNPEQHQAIGMQPNPEVPANHVMVVLQKGYELNG
HHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHCCCCCCCCCCCCHHHEEEHHHCCCCCCC
RLLRPAMVMVSQGGGVDTQA
EEHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA