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The map label for this gene is gcvA [H]

Identifier: 119775563

GI number: 119775563

Start: 2923122

End: 2924033

Strand: Reverse

Name: gcvA [H]

Synonym: Sama_2429

Alternate gene names: 119775563

Gene position: 2924033-2923122 (Counterclockwise)

Preceding gene: 119775565

Following gene: 119775562

Centisome position: 67.9

GC content: 53.62

Gene sequence:

>912_bases
ATGTCAAGACGACTTCCTCCGCTGAATGCCGTAAAGGCCTTTGAGGCCGCTGCACGGCACCTGAGTTTTACCCGCGCTGC
CGAAGAACTCTTTGTTACCCAGGCTGCTGTGAGCCATCAAATTAAGGCGCTGGAAGACTTTCTTGGATTAAAATTATTTC
GGCGAAAGAACCGTTCTTTGCTGCTCACCGAAGAAGGACAGAGCTACTTTCTCGACATCAAGGATATTTTTACCCAACTG
TCCGACGCCACCGATCGTTTGTTGGCCCGCAGTGCCATTGGCTCGTTGACCGTGAGTATGTCGCCCAGTTTCGCGATTCA
GTGGTTGGTGCCAAGGCTTGCCAAATTCAGTGAGAAAAATCCTGACATCGATGTGCGTATCAAGGCGGTGGACAGCGAAG
AAGGCTCTCTCACTGATGACGTGGATGTGGCCATTTACTATGGACAGGGCAGTTGGCCAGGTCTTCGGGCCGATAAACTG
CGCAACGAAGTGCTTATTCCAGTGTGTTCCCCCATGCTCCTCAATGGCCACAAGCCGCTTGAAACACCCGCAGATCTTAA
GCATCACACCCTGCTGCACGACATGAGTCGCCACGATTGGCAAGCCTGGTTTCGCCAGTGTGGCGTAACCGATGTAAACG
TGAACCAGGGGCCGATTTTCAGTCACTCTTCATTGGTATTGCAGGCCGCCGCCCATGGTCAGGGTGTGGCGCTGGGCTAC
AGCGTGCTGGCGCGCCCGGATATCAAAGCGGGTCGTTTGGTGGTGCCTTTTCAGGAAGTGTTGGTGAGTAAAGATGCCTA
TTATCTGGTGTGCCAGCAAAACCATGCCGAGCTTGGCAAAATTGTGGCGTTCCGCGAATGGATGCTGGACATGTTTGCCG
AGGAGTCACGCAGTGAACTCTTGCCCGCCTGA

Upstream 100 bases:

>100_bases
TGTTGGCGCAAAATTTACGCAAAAAGCCTTTCATTAACAAACGATAAAATGTAACAATGCGGATTAGAAAAACTAATTAA
AAAATGAGAGTGGGATCACC

Downstream 100 bases:

>100_bases
AGTGAACAACAACCCACAAACCCCTCTGCCATCCTGGCTTAAACTCGATGGCGTGCCGGGGGATGTGATGGTGCTCTTTG
CCCACGGCGCGGGTGCCGAT

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSRRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEDFLGLKLFRRKNRSLLLTEEGQSYFLDIKDIFTQL
SDATDRLLARSAIGSLTVSMSPSFAIQWLVPRLAKFSEKNPDIDVRIKAVDSEEGSLTDDVDVAIYYGQGSWPGLRADKL
RNEVLIPVCSPMLLNGHKPLETPADLKHHTLLHDMSRHDWQAWFRQCGVTDVNVNQGPIFSHSSLVLQAAAHGQGVALGY
SVLARPDIKAGRLVVPFQEVLVSKDAYYLVCQQNHAELGKIVAFREWMLDMFAEESRSELLPA

Sequences:

>Translated_303_residues
MSRRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEDFLGLKLFRRKNRSLLLTEEGQSYFLDIKDIFTQL
SDATDRLLARSAIGSLTVSMSPSFAIQWLVPRLAKFSEKNPDIDVRIKAVDSEEGSLTDDVDVAIYYGQGSWPGLRADKL
RNEVLIPVCSPMLLNGHKPLETPADLKHHTLLHDMSRHDWQAWFRQCGVTDVNVNQGPIFSHSSLVLQAAAHGQGVALGY
SVLARPDIKAGRLVVPFQEVLVSKDAYYLVCQQNHAELGKIVAFREWMLDMFAEESRSELLPA
>Mature_302_residues
SRRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEDFLGLKLFRRKNRSLLLTEEGQSYFLDIKDIFTQLS
DATDRLLARSAIGSLTVSMSPSFAIQWLVPRLAKFSEKNPDIDVRIKAVDSEEGSLTDDVDVAIYYGQGSWPGLRADKLR
NEVLIPVCSPMLLNGHKPLETPADLKHHTLLHDMSRHDWQAWFRQCGVTDVNVNQGPIFSHSSLVLQAAAHGQGVALGYS
VLARPDIKAGRLVVPFQEVLVSKDAYYLVCQQNHAELGKIVAFREWMLDMFAEESRSELLPA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=70.8474576271186, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1786448, Length=287, Percent_Identity=35.5400696864112, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1788706, Length=288, Percent_Identity=32.6388888888889, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI145693193, Length=301, Percent_Identity=26.2458471760797, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1786401, Length=237, Percent_Identity=28.6919831223629, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI157672245, Length=175, Percent_Identity=30.8571428571429, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081978, Length=256, Percent_Identity=29.6875, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789639, Length=194, Percent_Identity=27.319587628866, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787879, Length=130, Percent_Identity=32.3076923076923, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787128, Length=122, Percent_Identity=32.7868852459016, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788887, Length=174, Percent_Identity=29.8850574712644, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI145693105, Length=167, Percent_Identity=26.9461077844311, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790208, Length=241, Percent_Identity=26.5560165975104, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33878; Mature: 33747

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEDFLGLKLFRRKNRSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LLTEEGQSYFLDIKDIFTQLSDATDRLLARSAIGSLTVSMSPSFAIQWLVPRLAKFSEKN
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCC
PDIDVRIKAVDSEEGSLTDDVDVAIYYGQGSWPGLRADKLRNEVLIPVCSPMLLNGHKPL
CCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCEEEEECCHHHCCCCCCC
ETPADLKHHTLLHDMSRHDWQAWFRQCGVTDVNVNQGPIFSHSSLVLQAAAHGQGVALGY
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHEEECCCCCCEEEHH
SVLARPDIKAGRLVVPFQEVLVSKDAYYLVCQQNHAELGKIVAFREWMLDMFAEESRSEL
HHHCCCCCCCCEEEECHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHC
LPA
CCC
>Mature Secondary Structure 
SRRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEDFLGLKLFRRKNRSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LLTEEGQSYFLDIKDIFTQLSDATDRLLARSAIGSLTVSMSPSFAIQWLVPRLAKFSEKN
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCC
PDIDVRIKAVDSEEGSLTDDVDVAIYYGQGSWPGLRADKLRNEVLIPVCSPMLLNGHKPL
CCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCEEEEECCHHHCCCCCCC
ETPADLKHHTLLHDMSRHDWQAWFRQCGVTDVNVNQGPIFSHSSLVLQAAAHGQGVALGY
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHEEECCCCCCEEEHH
SVLARPDIKAGRLVVPFQEVLVSKDAYYLVCQQNHAELGKIVAFREWMLDMFAEESRSEL
HHHCCCCCCCCEEEECHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHC
LPA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]