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The map label for this gene is dosC [H]

Identifier: 119775552

GI number: 119775552

Start: 2910129

End: 2911685

Strand: Direct

Name: dosC [H]

Synonym: Sama_2418

Alternate gene names: 119775552

Gene position: 2910129-2911685 (Clockwise)

Preceding gene: 119775551

Following gene: 119775553

Centisome position: 67.58

GC content: 50.48

Gene sequence:

>1557_bases
ATGAAGGACGCCAACGAAGCGATTTCGCAAATCACGCTACTCAAACAGAAACTCCATTCTGCAAAGGTGGCGTTGGATGA
GCTCAATGAAGATCGCAACGCCAAGTTGCAAACCCTGCTGCAGTTCATTTCCAATCTGAGCCTGGCCTGCAAAGGGCAAA
ATCTGGAGTTGGATAACCGGCTTGCCAAGTTACGCCACCAACTGACTACCTACGAAAAGCTCGACGATGCCCTGCCGGAT
ATTGTCGAAGTGGAGCGCTTGCTCAAGGGCCAATACCACCATGTCATGGCACAGCTCGAAGACAGTCGAGCAGGCCTTGG
ACGGGTAGTACGCCAAATTCAACGTGTCGAGTCCGCACCCGACAAACTCAAAAAAGAAGTCGCCTACTTCAAACAGGATC
TCTCCAAGCCTCTGCACACCGTGTGGGAATATATCCCCAAGGTCGAAAAGTTGATGGCGTTTTACGAAGAGATACTGTCG
GCACAGTTTAATTCCGGCGAACCTTTGGCAGTGCTGCCCAAACACCGTCAACTGGCCCATGAGCTGGCCCAAATCATCTC
CGAAATCGAGTTTCGCAAAGACCAGCGCGATCAGGTGCTGGTGATTAAAGAAATGTTGGCCGGTGAGATAGATGTTGAGG
GGCTGATGGATGCCTATCAAACCATACTGTCCCTGCTGGTTGATAATATCGCCCGAGAGAAAAGCGCCTCTCAGGAATTC
CTCTATGCCCTGAATGATGCCTTGTCTGCAGTGCGCGAAGTCGTCTCAGACTCCTATAACCATACTCAGCGCAGTCATCA
GCTCAAGCATCAACTGAATCGTGAAATCAACTCCCGGGTTGATAACGTTGGCGAAGCCATAGTGGATATCGAAGATATCA
CTACGCTCAAGTTTCAAATTACCGAGCAGCTGTCCAGCCTGCGCTCGGCACTGTCCCGTAAAGAAGCCCTGGAGCAAAGA
GAACAAGCCTTGCTGCGCAAGTCGATGGAGACCATGCGCAAAGAACTCAATGAACTTGCTGAAGAAGCCAGCAACTATAA
AGAGCGCTTGTTTGAACAGCAAAAGCTGAATCTACTGGATACGCTCACCCAATTGCCCAATCGGGCTGCACTGGAAGAGC
GCATGGAGCAGGAATTCCGAAGCTTCCAGCGCCATAAGCACCCCCTGTGGGTAGCAGTGGCCGACATAGACCACTTCAAG
TCCATCAACGATAACTTTGGCCACAGCACCGGCGATAAGACTCTGCAGGTCATAGCCATGGCGCTGAAGAACTCGCTGCG
GGATACTGAGTTCGTGGCCCGCTACGGTGGTGAAGAATTTGTGCTGCTCATCCCTGAAGTATCAGATACTGACATAGTGC
AACTGCTGAACCGGGTGCGTGAGAAAGTAAAAAATATTCCATTTAAATTTAAAAATCAGAGAATTACAGTTACAGTATCC
ATAGGGGCCGCACGGGTAATGGATAATGAGCTCATTCACGAAACCTTCGAGCGAGCTGACGCGGCCCTCTACAAAGCCAA
ACATGAGAGCAGAGACAGGGTGATTATCGACTGCTAG

Upstream 100 bases:

>100_bases
TGCACTCTGGGTTGATTGCGATACCATTAATCCAGTGGACAAAATGCCGGTTCAGCATGAGAAAACACAGTCAGTTTATT
CGGTCAGGAAAGCATTCTGC

Downstream 100 bases:

>100_bases
CCCCGGTGGGGAGTAAGCAACTCCTGTCAACGCCCAAGGAGTTGCTATGATAGTGAAAGTCAGTCCCCGTGGTAGCATGG
ATCAGCTTTCCCAGCTGGAA

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MKDANEAISQITLLKQKLHSAKVALDELNEDRNAKLQTLLQFISNLSLACKGQNLELDNRLAKLRHQLTTYEKLDDALPD
IVEVERLLKGQYHHVMAQLEDSRAGLGRVVRQIQRVESAPDKLKKEVAYFKQDLSKPLHTVWEYIPKVEKLMAFYEEILS
AQFNSGEPLAVLPKHRQLAHELAQIISEIEFRKDQRDQVLVIKEMLAGEIDVEGLMDAYQTILSLLVDNIAREKSASQEF
LYALNDALSAVREVVSDSYNHTQRSHQLKHQLNREINSRVDNVGEAIVDIEDITTLKFQITEQLSSLRSALSRKEALEQR
EQALLRKSMETMRKELNELAEEASNYKERLFEQQKLNLLDTLTQLPNRAALEERMEQEFRSFQRHKHPLWVAVADIDHFK
SINDNFGHSTGDKTLQVIAMALKNSLRDTEFVARYGGEEFVLLIPEVSDTDIVQLLNRVREKVKNIPFKFKNQRITVTVS
IGAARVMDNELIHETFERADAALYKAKHESRDRVIIDC

Sequences:

>Translated_518_residues
MKDANEAISQITLLKQKLHSAKVALDELNEDRNAKLQTLLQFISNLSLACKGQNLELDNRLAKLRHQLTTYEKLDDALPD
IVEVERLLKGQYHHVMAQLEDSRAGLGRVVRQIQRVESAPDKLKKEVAYFKQDLSKPLHTVWEYIPKVEKLMAFYEEILS
AQFNSGEPLAVLPKHRQLAHELAQIISEIEFRKDQRDQVLVIKEMLAGEIDVEGLMDAYQTILSLLVDNIAREKSASQEF
LYALNDALSAVREVVSDSYNHTQRSHQLKHQLNREINSRVDNVGEAIVDIEDITTLKFQITEQLSSLRSALSRKEALEQR
EQALLRKSMETMRKELNELAEEASNYKERLFEQQKLNLLDTLTQLPNRAALEERMEQEFRSFQRHKHPLWVAVADIDHFK
SINDNFGHSTGDKTLQVIAMALKNSLRDTEFVARYGGEEFVLLIPEVSDTDIVQLLNRVREKVKNIPFKFKNQRITVTVS
IGAARVMDNELIHETFERADAALYKAKHESRDRVIIDC
>Mature_518_residues
MKDANEAISQITLLKQKLHSAKVALDELNEDRNAKLQTLLQFISNLSLACKGQNLELDNRLAKLRHQLTTYEKLDDALPD
IVEVERLLKGQYHHVMAQLEDSRAGLGRVVRQIQRVESAPDKLKKEVAYFKQDLSKPLHTVWEYIPKVEKLMAFYEEILS
AQFNSGEPLAVLPKHRQLAHELAQIISEIEFRKDQRDQVLVIKEMLAGEIDVEGLMDAYQTILSLLVDNIAREKSASQEF
LYALNDALSAVREVVSDSYNHTQRSHQLKHQLNREINSRVDNVGEAIVDIEDITTLKFQITEQLSSLRSALSRKEALEQR
EQALLRKSMETMRKELNELAEEASNYKERLFEQQKLNLLDTLTQLPNRAALEERMEQEFRSFQRHKHPLWVAVADIDHFK
SINDNFGHSTGDKTLQVIAMALKNSLRDTEFVARYGGEEFVLLIPEVSDTDIVQLLNRVREKVKNIPFKFKNQRITVTVS
IGAARVMDNELIHETFERADAALYKAKHESRDRVIIDC

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=195, Percent_Identity=35.3846153846154, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1787802, Length=160, Percent_Identity=36.875, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=33.5443037974684, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=37.888198757764, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=31.638418079096, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87081881, Length=121, Percent_Identity=42.1487603305785, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787262, Length=160, Percent_Identity=35, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1788381, Length=191, Percent_Identity=30.3664921465969, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788956, Length=188, Percent_Identity=30.8510638297872, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=27.9761904761905, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081974, Length=158, Percent_Identity=27.8481012658228, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081977, Length=107, Percent_Identity=36.4485981308411, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 59682; Mature: 59682

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDANEAISQITLLKQKLHSAKVALDELNEDRNAKLQTLLQFISNLSLACKGQNLELDNR
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHH
LAKLRHQLTTYEKLDDALPDIVEVERLLKGQYHHVMAQLEDSRAGLGRVVRQIQRVESAP
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
DKLKKEVAYFKQDLSKPLHTVWEYIPKVEKLMAFYEEILSAQFNSGEPLAVLPKHRQLAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHH
ELAQIISEIEFRKDQRDQVLVIKEMLAGEIDVEGLMDAYQTILSLLVDNIAREKSASQEF
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYALNDALSAVREVVSDSYNHTQRSHQLKHQLNREINSRVDNVGEAIVDIEDITTLKFQI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEQLSSLRSALSRKEALEQREQALLRKSMETMRKELNELAEEASNYKERLFEQQKLNLLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLTQLPNRAALEERMEQEFRSFQRHKHPLWVAVADIDHFKSINDNFGHSTGDKTLQVIAM
HHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHCCCCCCCCCCHHHHHHHHH
ALKNSLRDTEFVARYGGEEFVLLIPEVSDTDIVQLLNRVREKVKNIPFKFKNQRITVTVS
HHHHHCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEECCCEEEEEEE
IGAARVMDNELIHETFERADAALYKAKHESRDRVIIDC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MKDANEAISQITLLKQKLHSAKVALDELNEDRNAKLQTLLQFISNLSLACKGQNLELDNR
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHH
LAKLRHQLTTYEKLDDALPDIVEVERLLKGQYHHVMAQLEDSRAGLGRVVRQIQRVESAP
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
DKLKKEVAYFKQDLSKPLHTVWEYIPKVEKLMAFYEEILSAQFNSGEPLAVLPKHRQLAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHH
ELAQIISEIEFRKDQRDQVLVIKEMLAGEIDVEGLMDAYQTILSLLVDNIAREKSASQEF
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYALNDALSAVREVVSDSYNHTQRSHQLKHQLNREINSRVDNVGEAIVDIEDITTLKFQI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEQLSSLRSALSRKEALEQREQALLRKSMETMRKELNELAEEASNYKERLFEQQKLNLLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLTQLPNRAALEERMEQEFRSFQRHKHPLWVAVADIDHFKSINDNFGHSTGDKTLQVIAM
HHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHCCCCCCCCCCHHHHHHHHH
ALKNSLRDTEFVARYGGEEFVLLIPEVSDTDIVQLLNRVREKVKNIPFKFKNQRITVTVS
HHHHHCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEECCCEEEEEEE
IGAARVMDNELIHETFERADAALYKAKHESRDRVIIDC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]