Click here to switch to the map view.

The map label for this gene is cphE [H]

Identifier: 119775528

GI number: 119775528

Start: 2876743

End: 2878422

Strand: Direct

Name: cphE [H]

Synonym: Sama_2394

Alternate gene names: 119775528

Gene position: 2876743-2878422 (Clockwise)

Preceding gene: 119775524

Following gene: 119775530

Centisome position: 66.81

GC content: 58.1

Gene sequence:

>1680_bases
ATGACCCCAAAATTCCTCTCGTTTATCGGTGCTGTCGCCCTGTGGGTGTCAATCGCGCACCCGATTGCGTCCGCTGCGGC
CAAACCCTACAACCCTGACAAAGCACCGGAAACAGCCAGGGAACCCGTCCCAACACTGTCGCTGATGCTGGCCGGTGGCG
CCCTGTCTGTGTGCAGCTCCATGAACCCAAAGTACTGCACCGCTGACATACATTTCCCGGACAAGGCCAAAACCCGGCAT
TTGTATGAGCTGAGTGAACAAAGTCTGTCCCGCTTTCAGGTCACTCCGGCCTGGGCACGACTTAACGTCGATACCCAAAG
GGCATTGCTCGCCGCTCTTGGCTCTGCAGATAGAGCCCTTCTCGATATCGAGGCACTTCATGATCGGCTGGGCCCGCTTG
GCAATACACTCAATGATGCCAGCTATTTTGCCCTGCTCGACACCCTGGAGTGGGTACAGCTCACCCAGGAAGGAGAGCCA
AAGACAGAGCAGGTTCGGCTGGAACAGGGTAAAAATGCCCAGGGCGCCTCACACTTCGCCGCCTTTGTCAGCCGTGCGGC
ACGGCAAGTCGGCCACCAGCCACCGAAAATTGGCATAGTCACGGCATCTGCAAGGGATCCGTTTGAAGCAGCCAGCTTTT
ATCAACAAGCCTTTACTCAGGCGGGTGCAATAGCCCAGTGGCTACCGTTAGACAGCGCGTTGCAGCAAGCCTGGGGGTAT
GGCAATAGGGAAGCTGGATGCAAAGAGCTGGCAACCCTTGGTGAGAACTATCAGGGCTACCACGACAGGCAACGGGTGTA
CCCGACTCTGTGGGATAAATACAATCAGGTTTGTCTCAATCCAGAGGGGCTGCTGCGCACGCTGGCCGCCCTGGATGGCC
TCTTTCTCTCCGGCGGCGATCAATCCCTCACTTTGTCGGCTTGGCTGGGCGCCGATGGGCAACCGAGCGCCGCGCTGAAG
CTGATAAAAGAGCGATTCGCAAATGGAGAGCTTATCATCGGTGGCACCAGCGCCGGTACCGCCGTGATGGCATCGGGTGC
CATGATCACCGGGGGCACCAGCCAGGGCGCATTGGACTTTGGCGTGGTCGCCGCGCCCCCCGTCAGTGAGCGATGCGAAG
AACTGGGCTGCGAGGCGCTCAATCCTGCCGACACCCTCACCTACCGGCCCGGAGGCGGGCTGGGACTTTATCCGTTCGGG
GTGACAGATACCCATTTCAGTGAAAGGGACAGACAAATCAGGCTGGTACTGCTGCAGGATATAAGCCAAACCGGCCTTGG
GGTGGGCGTCGATGAAAATACCGCCCTGTGGCTATCCAAAGATGGCCAATATGGTGTGATCGAGGGCGAAGGCGGCGTCT
GGTTCAGTGAGCCTGCAGCCAAAGGTCAAACCCCGGATCCCCAAAAAGCGCAAATACACCAGAGCACGGCTCACAGCCAC
ATCATCCACTACCTTCCCCATGGTACCCGGCTGACGTTAACTCGCGATAACATCAGGGCGGAAGGCACGCAGGAAGAGTT
ACAAAAGGTCAGTTGCGACGGGTTTACAGCCGCTTGGCTGCCAAGCCAGGGATTCACCCTCCGCCACCTGGTTGACAGCC
AATGTCATCCTCAGGGTTATTACCAGTCACTGGGGCTGGAGCACAGCACAGAGATGTCGTCAGATAAGGCGACTCACTAA

Upstream 100 bases:

>100_bases
CCACCAAATGTGAAATCAATGTTGTTGACCGGTTAACAAGCTAGCCGTTATAGTGTCGGCCAGTCAATACGTGGCATGCA
AAATTTTTTAGAGCAGAGCC

Downstream 100 bases:

>100_bases
TCAGCGCTGTCCTGTCCCCTTCAGTCAGAGGCTGGCACTGTCTCCAGAACGGCAACGGCCTCTGGACACATTGGGTTGAC
GAACACCCCGACAGGCACTT

Product: cyanophycinase-related exopeptidase-like protein

Products: NA

Alternate protein names: Extracellular CGPase; CPHEpa; Extracellular cyanophycinase [H]

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MTPKFLSFIGAVALWVSIAHPIASAAAKPYNPDKAPETAREPVPTLSLMLAGGALSVCSSMNPKYCTADIHFPDKAKTRH
LYELSEQSLSRFQVTPAWARLNVDTQRALLAALGSADRALLDIEALHDRLGPLGNTLNDASYFALLDTLEWVQLTQEGEP
KTEQVRLEQGKNAQGASHFAAFVSRAARQVGHQPPKIGIVTASARDPFEAASFYQQAFTQAGAIAQWLPLDSALQQAWGY
GNREAGCKELATLGENYQGYHDRQRVYPTLWDKYNQVCLNPEGLLRTLAALDGLFLSGGDQSLTLSAWLGADGQPSAALK
LIKERFANGELIIGGTSAGTAVMASGAMITGGTSQGALDFGVVAAPPVSERCEELGCEALNPADTLTYRPGGGLGLYPFG
VTDTHFSERDRQIRLVLLQDISQTGLGVGVDENTALWLSKDGQYGVIEGEGGVWFSEPAAKGQTPDPQKAQIHQSTAHSH
IIHYLPHGTRLTLTRDNIRAEGTQEELQKVSCDGFTAAWLPSQGFTLRHLVDSQCHPQGYYQSLGLEHSTEMSSDKATH

Sequences:

>Translated_559_residues
MTPKFLSFIGAVALWVSIAHPIASAAAKPYNPDKAPETAREPVPTLSLMLAGGALSVCSSMNPKYCTADIHFPDKAKTRH
LYELSEQSLSRFQVTPAWARLNVDTQRALLAALGSADRALLDIEALHDRLGPLGNTLNDASYFALLDTLEWVQLTQEGEP
KTEQVRLEQGKNAQGASHFAAFVSRAARQVGHQPPKIGIVTASARDPFEAASFYQQAFTQAGAIAQWLPLDSALQQAWGY
GNREAGCKELATLGENYQGYHDRQRVYPTLWDKYNQVCLNPEGLLRTLAALDGLFLSGGDQSLTLSAWLGADGQPSAALK
LIKERFANGELIIGGTSAGTAVMASGAMITGGTSQGALDFGVVAAPPVSERCEELGCEALNPADTLTYRPGGGLGLYPFG
VTDTHFSERDRQIRLVLLQDISQTGLGVGVDENTALWLSKDGQYGVIEGEGGVWFSEPAAKGQTPDPQKAQIHQSTAHSH
IIHYLPHGTRLTLTRDNIRAEGTQEELQKVSCDGFTAAWLPSQGFTLRHLVDSQCHPQGYYQSLGLEHSTEMSSDKATH
>Mature_558_residues
TPKFLSFIGAVALWVSIAHPIASAAAKPYNPDKAPETAREPVPTLSLMLAGGALSVCSSMNPKYCTADIHFPDKAKTRHL
YELSEQSLSRFQVTPAWARLNVDTQRALLAALGSADRALLDIEALHDRLGPLGNTLNDASYFALLDTLEWVQLTQEGEPK
TEQVRLEQGKNAQGASHFAAFVSRAARQVGHQPPKIGIVTASARDPFEAASFYQQAFTQAGAIAQWLPLDSALQQAWGYG
NREAGCKELATLGENYQGYHDRQRVYPTLWDKYNQVCLNPEGLLRTLAALDGLFLSGGDQSLTLSAWLGADGQPSAALKL
IKERFANGELIIGGTSAGTAVMASGAMITGGTSQGALDFGVVAAPPVSERCEELGCEALNPADTLTYRPGGGLGLYPFGV
TDTHFSERDRQIRLVLLQDISQTGLGVGVDENTALWLSKDGQYGVIEGEGGVWFSEPAAKGQTPDPQKAQIHQSTAHSHI
IHYLPHGTRLTLTRDNIRAEGTQEELQKVSCDGFTAAWLPSQGFTLRHLVDSQCHPQGYYQSLGLEHSTEMSSDKATH

Specific function: Exopeptidase that catalyzes the hydrolytic cleavage of multi-L-arginyl-poly-L-aspartic acid (cyanophycin; a water- insoluble reserve polymer) into aspartate-arginine dipeptides [H]

COG id: COG4242

COG function: function code QP; Cyanophycinase and related exopeptidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S51 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005320
- InterPro:   IPR011811 [H]

Pfam domain/function: PF03575 Peptidase_S51 [H]

EC number: =3.4.15.6 [H]

Molecular weight: Translated: 60280; Mature: 60149

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPKFLSFIGAVALWVSIAHPIASAAAKPYNPDKAPETAREPVPTLSLMLAGGALSVCSS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCHHHHCCCHHHHHHC
MNPKYCTADIHFPDKAKTRHLYELSEQSLSRFQVTPAWARLNVDTQRALLAALGSADRAL
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEECCCHHEEECCCHHHHHHHHHCCCCHHH
LDIEALHDRLGPLGNTLNDASYFALLDTLEWVQLTQEGEPKTEQVRLEQGKNAQGASHFA
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHH
AFVSRAARQVGHQPPKIGIVTASARDPFEAASFYQQAFTQAGAIAQWLPLDSALQQAWGY
HHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHCCC
GNREAGCKELATLGENYQGYHDRQRVYPTLWDKYNQVCLNPEGLLRTLAALDGLFLSGGD
CCCCHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHCCEEECHHHHHHHHHHHCCEEECCCC
QSLTLSAWLGADGQPSAALKLIKERFANGELIIGGTSAGTAVMASGAMITGGTSQGALDF
CEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHEECCEEEECCCCCCCCEE
GVVAAPPVSERCEELGCEALNPADTLTYRPGGGLGLYPFGVTDTHFSERDRQIRLVLLQD
CEEECCCHHHHHHHCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCCEEEEEEEHH
ISQTGLGVGVDENTALWLSKDGQYGVIEGEGGVWFSEPAAKGQTPDPQKAQIHQSTAHSH
HHHCCCCCCCCCCCEEEEECCCCEEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHH
IIHYLPHGTRLTLTRDNIRAEGTQEELQKVSCDGFTAAWLPSQGFTLRHLVDSQCHPQGY
HEEECCCCCEEEEECCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCHHHHHCCCCCCCHH
YQSLGLEHSTEMSSDKATH
HHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TPKFLSFIGAVALWVSIAHPIASAAAKPYNPDKAPETAREPVPTLSLMLAGGALSVCSS
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCHHHHCCCHHHHHHC
MNPKYCTADIHFPDKAKTRHLYELSEQSLSRFQVTPAWARLNVDTQRALLAALGSADRAL
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEECCCHHEEECCCHHHHHHHHHCCCCHHH
LDIEALHDRLGPLGNTLNDASYFALLDTLEWVQLTQEGEPKTEQVRLEQGKNAQGASHFA
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHH
AFVSRAARQVGHQPPKIGIVTASARDPFEAASFYQQAFTQAGAIAQWLPLDSALQQAWGY
HHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHCCC
GNREAGCKELATLGENYQGYHDRQRVYPTLWDKYNQVCLNPEGLLRTLAALDGLFLSGGD
CCCCHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHCCEEECHHHHHHHHHHHCCEEECCCC
QSLTLSAWLGADGQPSAALKLIKERFANGELIIGGTSAGTAVMASGAMITGGTSQGALDF
CEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHEECCEEEECCCCCCCCEE
GVVAAPPVSERCEELGCEALNPADTLTYRPGGGLGLYPFGVTDTHFSERDRQIRLVLLQD
CEEECCCHHHHHHHCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCCEEEEEEEHH
ISQTGLGVGVDENTALWLSKDGQYGVIEGEGGVWFSEPAAKGQTPDPQKAQIHQSTAHSH
HHHCCCCCCCCCCCEEEEECCCCEEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHH
IIHYLPHGTRLTLTRDNIRAEGTQEELQKVSCDGFTAAWLPSQGFTLRHLVDSQCHPQGY
HEEECCCCCEEEEECCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCHHHHHCCCCCCCHH
YQSLGLEHSTEMSSDKATH
HHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11986309 [H]