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The map label for this gene is rlmN

Identifier: 119775502

GI number: 119775502

Start: 2845058

End: 2846179

Strand: Reverse

Name: rlmN

Synonym: Sama_2368

Alternate gene names: 119775502

Gene position: 2846179-2845058 (Counterclockwise)

Preceding gene: 119775505

Following gene: 119775501

Centisome position: 66.1

GC content: 50.8

Gene sequence:

>1122_bases
ATGAGTGAAAAGAAGATCAATTTGCTGGACCTGGATTTGCCGGGACTGAAAGCGCTGCTGACTGAAATGGGCGAAAAACC
CTTTCGTGCCCAGCAGATAATGCAATGGATCTATCATTTCGGTGTCAGTGACTTCGAACAGATGACCAACATTAATAAGG
CCATGCGTGCCAAGCTTGCCGCACGTTGTGAAATTGTTGCGCCTGAAATCACCAGTTACCAAAAATCTTCTGATGGCACT
ATTAAGTTTGCCATTAACGTAGGGCAGGGGCAGGAAGTTGAGACGGTGTACATTCCCGAGGATGACCGCGCCACCCTGTG
TGTATCATCTCAGGTGGGTTGTGCCCTTGAGTGTACCTTTTGCTCCACCGCACAGCAGGGGTTTAACCGTAACCTGACCG
TATCTGAAATTGTTGGCCAGATTTGGCGGGTATCGCACTTTTTGGGCTTCCAGAAAGAAACCGGCGAGCGCCCCATTTCC
AACGTGGTGATGATGGGTATGGGTGAGCCTTTGCTTAACCTCGCCAATGTGGTCCCTGCCATGAATATTATGCTGGACGA
CTACGGTTTTGGTTTGTCCAAGCGCCGTGTGACTTTGTCTACCTCCGGTGTGGTGCCTGCGCTGGATAAACTTGGCGACG
TAATTGATGTGGCGTTGGCTGTCAGTATCCATGCACCCAATGACGAGCTTAGGGATGTGCTGGTGCCCATCAATAAAAAG
TATCCGCTGCAGGAGTTTTTGGCTGCCATCCGTCGTTACCTCGAGAAGTCCAACGCCAATCGTGGTCGCGTGACTCTCGA
GTACGTGATGCTGGACCATATCAATGACAGCACCGACCAGGCCCATGAATTGGCCAAGTTGATGAAGGATACTCCCTGTA
AAATCAACCTGATCCCCTTCAACCCCTATCCAGGTTCCCCTTACGGGCGCTCGTCCAACTCCCGTATTGACCGTTTTGCC
AAGGTGTTGATGGAATACGACTTGACCGTAATAGTGCGTAAAACCCGCGGTGATGATATCGATGCGGCCTGTGGTCAGCT
CGCCGGGGATATCCGTGATCGCACCAAGCGTCTGGCAAAAAAACGCATGCAAGAAAGCCAGATTTCAGTCACAATGAATT
AA

Upstream 100 bases:

>100_bases
CATGGTAGCATAGACTTGGTTTGGCTGCTGGCGAATTTCGTGATGGATCGGTATAATTCGCGGCCCCCGTGGTGGGAATA
ACTGGTTTGAGGTTTGTCTG

Downstream 100 bases:

>100_bases
CCCATTGTTTGCACAGATTAATAGGTCTGAACATGAAGCAAGGATTGCCTTCTTTAACCCTGATCGCCATCCTGTCCGGA
CTGGTGACGGGCTGCGTTAC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSEKKINLLDLDLPGLKALLTEMGEKPFRAQQIMQWIYHFGVSDFEQMTNINKAMRAKLAARCEIVAPEITSYQKSSDGT
IKFAINVGQGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTAQQGFNRNLTVSEIVGQIWRVSHFLGFQKETGERPIS
NVVMMGMGEPLLNLANVVPAMNIMLDDYGFGLSKRRVTLSTSGVVPALDKLGDVIDVALAVSIHAPNDELRDVLVPINKK
YPLQEFLAAIRRYLEKSNANRGRVTLEYVMLDHINDSTDQAHELAKLMKDTPCKINLIPFNPYPGSPYGRSSNSRIDRFA
KVLMEYDLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKRMQESQISVTMN

Sequences:

>Translated_373_residues
MSEKKINLLDLDLPGLKALLTEMGEKPFRAQQIMQWIYHFGVSDFEQMTNINKAMRAKLAARCEIVAPEITSYQKSSDGT
IKFAINVGQGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTAQQGFNRNLTVSEIVGQIWRVSHFLGFQKETGERPIS
NVVMMGMGEPLLNLANVVPAMNIMLDDYGFGLSKRRVTLSTSGVVPALDKLGDVIDVALAVSIHAPNDELRDVLVPINKK
YPLQEFLAAIRRYLEKSNANRGRVTLEYVMLDHINDSTDQAHELAKLMKDTPCKINLIPFNPYPGSPYGRSSNSRIDRFA
KVLMEYDLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKRMQESQISVTMN
>Mature_372_residues
SEKKINLLDLDLPGLKALLTEMGEKPFRAQQIMQWIYHFGVSDFEQMTNINKAMRAKLAARCEIVAPEITSYQKSSDGTI
KFAINVGQGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTAQQGFNRNLTVSEIVGQIWRVSHFLGFQKETGERPISN
VVMMGMGEPLLNLANVVPAMNIMLDDYGFGLSKRRVTLSTSGVVPALDKLGDVIDVALAVSIHAPNDELRDVLVPINKKY
PLQEFLAAIRRYLEKSNANRGRVTLEYVMLDHINDSTDQAHELAKLMKDTPCKINLIPFNPYPGSPYGRSSNSRIDRFAK
VLMEYDLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKRMQESQISVTMN

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=366, Percent_Identity=73.4972677595628, Blast_Score=581, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_SHEAM (A1S866)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928242.1
- ProteinModelPortal:   A1S866
- STRING:   A1S866
- GeneID:   4604617
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2368
- NMPDR:   fig|326297.7.peg.2218
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   A1S866
- ProtClustDB:   PRK11194
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 41671; Mature: 41539

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKKINLLDLDLPGLKALLTEMGEKPFRAQQIMQWIYHFGVSDFEQMTNINKAMRAKLA
CCCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ARCEIVAPEITSYQKSSDGTIKFAINVGQGQEVETVYIPEDDRATLCVSSQVGCALECTF
HHHEEECCCHHCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEE
CSTAQQGFNRNLTVSEIVGQIWRVSHFLGFQKETGERPISNVVMMGMGEPLLNLANVVPA
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHCCCCHHHHHHHHHHH
MNIMLDDYGFGLSKRRVTLSTSGVVPALDKLGDVIDVALAVSIHAPNDELRDVLVPINKK
HHHEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHEEHHEEEEECCCHHHHHHHCCCCCC
YPLQEFLAAIRRYLEKSNANRGRVTLEYVMLDHINDSTDQAHELAKLMKDTPCKINLIPF
CCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEC
NPYPGSPYGRSSNSRIDRFAKVLMEYDLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAK
CCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KRMQESQISVTMN
HHHHHCCEEEEEC
>Mature Secondary Structure 
SEKKINLLDLDLPGLKALLTEMGEKPFRAQQIMQWIYHFGVSDFEQMTNINKAMRAKLA
CCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ARCEIVAPEITSYQKSSDGTIKFAINVGQGQEVETVYIPEDDRATLCVSSQVGCALECTF
HHHEEECCCHHCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEE
CSTAQQGFNRNLTVSEIVGQIWRVSHFLGFQKETGERPISNVVMMGMGEPLLNLANVVPA
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHCCCCHHHHHHHHHHH
MNIMLDDYGFGLSKRRVTLSTSGVVPALDKLGDVIDVALAVSIHAPNDELRDVLVPINKK
HHHEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHEEHHEEEEECCCHHHHHHHCCCCCC
YPLQEFLAAIRRYLEKSNANRGRVTLEYVMLDHINDSTDQAHELAKLMKDTPCKINLIPF
CCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEC
NPYPGSPYGRSSNSRIDRFAKVLMEYDLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAK
CCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KRMQESQISVTMN
HHHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA