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The map label for this gene is rfbB [H]

Identifier: 119775377

GI number: 119775377

Start: 2705086

End: 2706219

Strand: Reverse

Name: rfbB [H]

Synonym: Sama_2242

Alternate gene names: 119775377

Gene position: 2706219-2705086 (Counterclockwise)

Preceding gene: 119775378

Following gene: 119775376

Centisome position: 62.85

GC content: 50.53

Gene sequence:

>1134_bases
ATGAAGATTCTTGTTACAGGTGGCTGCGGCTTTATCGGTTCGGCGGTAATCAGACATTTAATCAGTCACACTGAACACAG
CGTGGTGAATGTCGACAAGCTAACTTATGCGGCAAATCTGTTATCTGTCGCTGAGGCAGCAGAAAGCGCCAGATACGCGC
TTGAGCGTGTCGACATCTGTGACAGGCAGGCACTTGGGGCGGTTTTTGCTCGCCATCAGCCCGATGTGGTGATGCATCTA
GCTGCGGAAACCCATGTCGATCGCTCTATAACAGGTGCGGATGCCTTTATCCAAACCAACATAGTGGGCACTTACACCTT
GTTGGAAGTTGCCCGTGAATATTGGCGGCAGCTCGACGACATGCGTAAAAACGCATTTCGATTTCATCATGTTTCGACCG
ACGAAGTATTTGGGGATCTGGCTGACCAGGTTAATGCTGAATGTTTGGTTGAGAATGCTGAATTGAACACTGATGGTGCA
AGTTCACCATTGAGCATTCATGATTCAACGTTAAAAACAGCCGCGCCATTTACCGAAAGCAGTGCTTATGCGCCGTCATC
ACCTTATTCCGCCTCCAAGGCCTCAAGCGATCATCTGGTTAGGGCATGGCACCGGACTTATGGTTTGCCAATCGTTGTCA
GCAACTGCTCCAATAACTATGGGCCATTTCAATACCCGGAAAAACTCATTCCGCTGACTATTCAAAATGCACTGGCGGGA
AAGGCGTTACCCGTGTATGGCCAAGGAAACCAAAGCCGCGATTGGCTTTATGTGGAGGACCACGCACGGGCACTGCTGGC
CGTAATGACCCGTGGGCGGGTAGGCGAGACATATAACATCGGCGGCAATAGCGAGCGTCAAAATCTCGAGGTGGTTAAAA
GCATTTGTGCGATCCTCGATGAGTTGGCTCCGTTGCATGAACGCACACAGGAGCTCAATCCTCATCATGCATCCCCCAAT
ACTCTACATTACGAGAGTCTCATCACTTTCGTGGCCGACAGACCCGGCCATGATGTGCGCTACGCCATTGATGCCAGCAA
AATTACCGAGCAGCTTGGCTGGAAACCGCAGGAGACCTTTGAATCCGGTCTGCGTAAAACGGTTGAGTGGTATGTTCTGA
ATGGTGAATGGTGA

Upstream 100 bases:

>100_bases
GAACCTAGATCCTGGCAGTCGCGCAGCGACGCTCTAGAAGCCCAAGCGCTTTGCTTTTTCTGGCAGGGCTTTAGCCCGCT
CTGCTTTATTGAGGTTGTCC

Downstream 100 bases:

>100_bases
ATGGTGAATGGTGAATGGTGAATGGTTAATTGTGAACTTATGGAGTAACGCTGATGCCTGGTAACGCCGTGCTTTATAAG
TCGTTTCAATTTGCTGTTAG

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKILVTGGCGFIGSAVIRHLISHTEHSVVNVDKLTYAANLLSVAEAAESARYALERVDICDRQALGAVFARHQPDVVMHL
AAETHVDRSITGADAFIQTNIVGTYTLLEVAREYWRQLDDMRKNAFRFHHVSTDEVFGDLADQVNAECLVENAELNTDGA
SSPLSIHDSTLKTAAPFTESSAYAPSSPYSASKASSDHLVRAWHRTYGLPIVVSNCSNNYGPFQYPEKLIPLTIQNALAG
KALPVYGQGNQSRDWLYVEDHARALLAVMTRGRVGETYNIGGNSERQNLEVVKSICAILDELAPLHERTQELNPHHASPN
TLHYESLITFVADRPGHDVRYAIDASKITEQLGWKPQETFESGLRKTVEWYVLNGEW

Sequences:

>Translated_377_residues
MKILVTGGCGFIGSAVIRHLISHTEHSVVNVDKLTYAANLLSVAEAAESARYALERVDICDRQALGAVFARHQPDVVMHL
AAETHVDRSITGADAFIQTNIVGTYTLLEVAREYWRQLDDMRKNAFRFHHVSTDEVFGDLADQVNAECLVENAELNTDGA
SSPLSIHDSTLKTAAPFTESSAYAPSSPYSASKASSDHLVRAWHRTYGLPIVVSNCSNNYGPFQYPEKLIPLTIQNALAG
KALPVYGQGNQSRDWLYVEDHARALLAVMTRGRVGETYNIGGNSERQNLEVVKSICAILDELAPLHERTQELNPHHASPN
TLHYESLITFVADRPGHDVRYAIDASKITEQLGWKPQETFESGLRKTVEWYVLNGEW
>Mature_377_residues
MKILVTGGCGFIGSAVIRHLISHTEHSVVNVDKLTYAANLLSVAEAAESARYALERVDICDRQALGAVFARHQPDVVMHL
AAETHVDRSITGADAFIQTNIVGTYTLLEVAREYWRQLDDMRKNAFRFHHVSTDEVFGDLADQVNAECLVENAELNTDGA
SSPLSIHDSTLKTAAPFTESSAYAPSSPYSASKASSDHLVRAWHRTYGLPIVVSNCSNNYGPFQYPEKLIPLTIQNALAG
KALPVYGQGNQSRDWLYVEDHARALLAVMTRGRVGETYNIGGNSERQNLEVVKSICAILDELAPLHERTQELNPHHASPN
TLHYESLITFVADRPGHDVRYAIDASKITEQLGWKPQETFESGLRKTVEWYVLNGEW

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=374, Percent_Identity=32.3529411764706, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI42516563, Length=372, Percent_Identity=22.0430107526882, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788353, Length=379, Percent_Identity=62.7968337730871, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI48994969, Length=376, Percent_Identity=62.7659574468085, Blast_Score=481, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17568069, Length=374, Percent_Identity=35.5614973262032, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=368, Percent_Identity=31.5217391304348, Blast_Score=174, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6319493, Length=324, Percent_Identity=24.3827160493827, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 41785; Mature: 41785

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGCGFIGSAVIRHLISHTEHSVVNVDKLTYAANLLSVAEAAESARYALERVDIC
CEEEEECCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRQALGAVFARHQPDVVMHLAAETHVDRSITGADAFIQTNIVGTYTLLEVAREYWRQLDD
HHHHHHHHHHCCCCCCEEEEHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHH
MRKNAFRFHHVSTDEVFGDLADQVNAECLVENAELNTDGASSPLSIHDSTLKTAAPFTES
HHHHHEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEECCCHHHHCCCCCCC
SAYAPSSPYSASKASSDHLVRAWHRTYGLPIVVSNCSNNYGPFQYPEKLIPLTIQNALAG
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHCCCHHHHHHCC
KALPVYGQGNQSRDWLYVEDHARALLAVMTRGRVGETYNIGGNSERQNLEVVKSICAILD
CCEEEECCCCCCCCEEEEHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHH
ELAPLHERTQELNPHHASPNTLHYESLITFVADRPGHDVRYAIDASKITEQLGWKPQETF
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHCCCCHHHH
ESGLRKTVEWYVLNGEW
HHHHHHEEEEEEECCCC
>Mature Secondary Structure
MKILVTGGCGFIGSAVIRHLISHTEHSVVNVDKLTYAANLLSVAEAAESARYALERVDIC
CEEEEECCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRQALGAVFARHQPDVVMHLAAETHVDRSITGADAFIQTNIVGTYTLLEVAREYWRQLDD
HHHHHHHHHHCCCCCCEEEEHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHH
MRKNAFRFHHVSTDEVFGDLADQVNAECLVENAELNTDGASSPLSIHDSTLKTAAPFTES
HHHHHEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEECCCHHHHCCCCCCC
SAYAPSSPYSASKASSDHLVRAWHRTYGLPIVVSNCSNNYGPFQYPEKLIPLTIQNALAG
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHCCCHHHHHHCC
KALPVYGQGNQSRDWLYVEDHARALLAVMTRGRVGETYNIGGNSERQNLEVVKSICAILD
CCEEEECCCCCCCCEEEEHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHH
ELAPLHERTQELNPHHASPNTLHYESLITFVADRPGHDVRYAIDASKITEQLGWKPQETF
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHCCCCHHHH
ESGLRKTVEWYVLNGEW
HHHHHHEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503 [H]