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The map label for this gene is rfbB [C]

Identifier: 119775370

GI number: 119775370

Start: 2699039

End: 2700043

Strand: Reverse

Name: rfbB [C]

Synonym: Sama_2235

Alternate gene names: 119775370

Gene position: 2700043-2699039 (Counterclockwise)

Preceding gene: 119775371

Following gene: 119775368

Centisome position: 62.7

GC content: 53.23

Gene sequence:

>1005_bases
ATGAAAATATTGGTCACAGGCGCCGCCGGATTTATTGGCGCTGCCGCATGCAAAAGGCTGCTTTCGCAGGGACATCAGGT
TCATGGCATCGATAATCTTAACGATTACTACGATGTCTCTTTGAAGCAGGCGAGGTTGGCTGCTCTCGCATCTCATCCCC
ACTTTCAATTTCAGCGAATGGAGCTTGCCGACCGCGGGGCGATTGCAACGCTTTTTTCGACCATAAAGCCCGATTGGGTG
CTGCATCTTGGTGCTCAGGCGGGGGTTCGTTACTCCATCGACAATCCCCATGCTTATGCCGATGCCAATTTGGTGGGGCA
TCTCAATATTCTTGAAGGGTGTCGCCAAACCGGCGTAAAGCATCTGGTGTATGCGTCCTCAAGCTCAGTGTACGGGCTTA
ACAAAAAGCTGCCCTTCGCCACCAGTGACTCGGTAGATCACCCCGTTTCCCTCTATGCGGCCACTAAAAAGGCCAATGAG
TTGATGTCACACAGTTATGCCCACCTTTATGGCGTGCCCTGCACGGGGCTGCGGTTTTTTACCGTTTATGGACCTTGGGG
CCGACCGGATATGGCGCCCATGTTGTTTGCCAGGGCGATTATGGCAGGTGAGCCCATCAAGGTGTTTAATCAAGGCGATA
TGAGCCGCGACTTTACCTTTATCGACGATATCATTGAGGGCGTAATAGGCGTGCTGCCGCTGCCGCCAAGCACATCGTCT
CAGTGGTCGGTAGAAAGTGGCTCCAGCAGTGAAAGCTCAGCGCCTTACCGGGTACTGAATATCGGCCACGGCAGCCCGGT
ATCTCTGATGCACTTTATCGAAACCCTCGAAAATGCCCTTGGCCGTAAGGCCATCAAGCAGTTTTTACCGATGCAGGACG
GCGATGTAAAAGCCACCTGGGCGGATACAGAAGATTTGTTTGCCATCACGGGCGTGCGCCCCAAGGTGGGGATAGAGCAG
GGGGTAAAAGCCTTTGCCGACTGGTATCTAAACTACTATCACTGA

Upstream 100 bases:

>100_bases
TATTACGGTATTGGCCGTGGTCGCTCGGTAAAGCATCCTGATGTGCGCAAGGTACTCTGATGACTTATGGCAGTGTAAGG
AGAATTAAGTGAGCCAATCC

Downstream 100 bases:

>100_bases
TAACGCCGCAAGCGGGCTGCCAAGACGCTTTTCGGTCGACACTTAGTGTATCTGGGGCGCAGAGTAAACACTGCTTCTAG
AATCTAGAATCTAGAATCTA

Product: NAD dependent epimerase/dehydratase family protein

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKILVTGAAGFIGAAACKRLLSQGHQVHGIDNLNDYYDVSLKQARLAALASHPHFQFQRMELADRGAIATLFSTIKPDWV
LHLGAQAGVRYSIDNPHAYADANLVGHLNILEGCRQTGVKHLVYASSSSVYGLNKKLPFATSDSVDHPVSLYAATKKANE
LMSHSYAHLYGVPCTGLRFFTVYGPWGRPDMAPMLFARAIMAGEPIKVFNQGDMSRDFTFIDDIIEGVIGVLPLPPSTSS
QWSVESGSSSESSAPYRVLNIGHGSPVSLMHFIETLENALGRKAIKQFLPMQDGDVKATWADTEDLFAITGVRPKVGIEQ
GVKAFADWYLNYYH

Sequences:

>Translated_334_residues
MKILVTGAAGFIGAAACKRLLSQGHQVHGIDNLNDYYDVSLKQARLAALASHPHFQFQRMELADRGAIATLFSTIKPDWV
LHLGAQAGVRYSIDNPHAYADANLVGHLNILEGCRQTGVKHLVYASSSSVYGLNKKLPFATSDSVDHPVSLYAATKKANE
LMSHSYAHLYGVPCTGLRFFTVYGPWGRPDMAPMLFARAIMAGEPIKVFNQGDMSRDFTFIDDIIEGVIGVLPLPPSTSS
QWSVESGSSSESSAPYRVLNIGHGSPVSLMHFIETLENALGRKAIKQFLPMQDGDVKATWADTEDLFAITGVRPKVGIEQ
GVKAFADWYLNYYH
>Mature_334_residues
MKILVTGAAGFIGAAACKRLLSQGHQVHGIDNLNDYYDVSLKQARLAALASHPHFQFQRMELADRGAIATLFSTIKPDWV
LHLGAQAGVRYSIDNPHAYADANLVGHLNILEGCRQTGVKHLVYASSSSVYGLNKKLPFATSDSVDHPVSLYAATKKANE
LMSHSYAHLYGVPCTGLRFFTVYGPWGRPDMAPMLFARAIMAGEPIKVFNQGDMSRDFTFIDDIIEGVIGVLPLPPSTSS
QWSVESGSSSESSAPYRVLNIGHGSPVSLMHFIETLENALGRKAIKQFLPMQDGDVKATWADTEDLFAITGVRPKVGIEQ
GVKAFADWYLNYYH

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=335, Percent_Identity=24.4776119402985, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI7657641, Length=350, Percent_Identity=24.2857142857143, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI56237023, Length=322, Percent_Identity=26.7080745341615, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI56118217, Length=322, Percent_Identity=26.7080745341615, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI189083684, Length=322, Percent_Identity=26.7080745341615, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=24.2339832869081, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI48994969, Length=234, Percent_Identity=30.3418803418803, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786974, Length=315, Percent_Identity=25.7142857142857, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788366, Length=185, Percent_Identity=29.1891891891892, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788589, Length=246, Percent_Identity=26.8292682926829, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71982035, Length=356, Percent_Identity=26.4044943820225, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71982038, Length=358, Percent_Identity=26.2569832402235, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17568069, Length=237, Percent_Identity=25.3164556962025, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI133901786, Length=187, Percent_Identity=31.5508021390374, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17539424, Length=187, Percent_Identity=31.5508021390374, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI133901788, Length=187, Percent_Identity=31.5508021390374, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17539422, Length=187, Percent_Identity=31.5508021390374, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI133901790, Length=187, Percent_Identity=31.5508021390374, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17507723, Length=187, Percent_Identity=30.4812834224599, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17539532, Length=237, Percent_Identity=23.6286919831224, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=243, Percent_Identity=30.4526748971193, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19923002, Length=348, Percent_Identity=25.2873563218391, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21356223, Length=337, Percent_Identity=25.2225519287834, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 36532; Mature: 36532

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGAAGFIGAAACKRLLSQGHQVHGIDNLNDYYDVSLKQARLAALASHPHFQFQRM
CEEEEECCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEHHH
ELADRGAIATLFSTIKPDWVLHLGAQAGVRYSIDNPHAYADANLVGHLNILEGCRQTGVK
HHHHCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCEECCCCEEHHHHHHHHHHCCCC
HLVYASSSSVYGLNKKLPFATSDSVDHPVSLYAATKKANELMSHSYAHLYGVPCTGLRFF
EEEEECCCCEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCHHEECCCCCCEEEE
TVYGPWGRPDMAPMLFARAIMAGEPIKVFNQGDMSRDFTFIDDIIEGVIGVLPLPPSTSS
EEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
QWSVESGSSSESSAPYRVLNIGHGSPVSLMHFIETLENALGRKAIKQFLPMQDGDVKATW
CEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
ADTEDLFAITGVRPKVGIEQGVKAFADWYLNYYH
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKILVTGAAGFIGAAACKRLLSQGHQVHGIDNLNDYYDVSLKQARLAALASHPHFQFQRM
CEEEEECCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEHHH
ELADRGAIATLFSTIKPDWVLHLGAQAGVRYSIDNPHAYADANLVGHLNILEGCRQTGVK
HHHHCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCEECCCCEEHHHHHHHHHHCCCC
HLVYASSSSVYGLNKKLPFATSDSVDHPVSLYAATKKANELMSHSYAHLYGVPCTGLRFF
EEEEECCCCEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCHHEECCCCCCEEEE
TVYGPWGRPDMAPMLFARAIMAGEPIKVFNQGDMSRDFTFIDDIIEGVIGVLPLPPSTSS
EEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
QWSVESGSSSESSAPYRVLNIGHGSPVSLMHFIETLENALGRKAIKQFLPMQDGDVKATW
CEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
ADTEDLFAITGVRPKVGIEQGVKAFADWYLNYYH
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]