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The map label for this gene is apr [H]

Identifier: 119775320

GI number: 119775320

Start: 2643189

End: 2645600

Strand: Direct

Name: apr [H]

Synonym: Sama_2185

Alternate gene names: 119775320

Gene position: 2643189-2645600 (Clockwise)

Preceding gene: 119775319

Following gene: 119775330

Centisome position: 61.38

GC content: 56.05

Gene sequence:

>2412_bases
ATGACAATGAAACACAAAAAAACTTTAGCCCTGGGTTTATCGACCCTCGCACTGGCAGTCTCTGCCGGTGTTCATGCCGC
ACCTACCGACGCAGTCTTCGCCAAAGGCGAAAACTCCCCGCTACCAAAGCGCTATGTAGTTAAATTCAAAAACAATGCCG
CCACCGAGCTTTACAATGCTGAAGACACACTCAACAGCATGCAGTATCAGCCACGCAGCCATGAAGTCTATGGTCACCAC
CGTGCCCTGAATGCCGCCAGTGCCAAAGAAATGAAGCGTATCGGTCGCAGCAACGCTTACACCGTGAAGCTGGACAACAA
TGGCATCAAGGCCCTGCGCGCCCGTGCCGATGTTGAATTTGTGGAAGAAGACGTGCCACGTCGTCTGCTTGCTGAAACCA
CCCCCTGGGGTCAAACCTTTGTTGGTGCAACTCAGCTGGCCGATAACCTGAGCGGCAACCGCACCATCTGTATCATTGAC
TCAGGCTATGACCGTGGTCATGCCGATCTGTCAGGCAACAATGTGACCGGTACCAATAACTCAGGCACCGGCAACTGGTT
CGAACCAGGCAACAACAACGCCCACGGCACCCACGTAGCTGGCACCATTGCGGCCATTGCCAACGGTGAAGGCGTGGTGG
GCGTGCTGCCAAACCAAAATGCCAATATCCATATTATCAAAGTGTTCAACGAAGCCGGCTGGGGTTATTCTTCAAGCCTG
GTCAGTGCCGTGGACACCTGTGTGGCTAACGGCGCCAACGTCGTCAGTATGTCTCTGGGCGGTGCCAGCTCAAGTACCAC
TGAGCGCAACGCCCTGGCGGCCCACTACAACAACGGCGTGCTGCTGATTGCTGCCGCGGGTAACGACGGTGATAACACCC
ACAGCTACCCGGCATCTTACGATAGCGTGGTATCGGTTGCTGCCGTAGACAGCACCAAGCAGCATGCTGCGTTTTCTCAG
TACACCAATCAAGTGGAAGTATCCGGCCCCGGCGAAGCCATTCTGTCTACCGTGACCCGCGGCGAAGGCCGTTTGTCTGA
CATCGTGGTTGCTGGTCAGTCTTACTTCAACAACGGTGTTGTACCTCATAACCGCTTGGTAAAATCCGGTACCAGCTATG
CTCCAGCACCAATCAACGGCACTGTGACGGCCACCCTGGCCGAGTGCAGCGTGTCTGGCAGCACCTTTAACTGTGGCAAC
ATGGCCGGCAAGGTGTGTCTGGTTGAGCGTGTAGGCAACCAGGGTTCAAGCTACCCTGAAATCAACGCGGCCAAGGCCTG
TCAAAACGCCGGTGCTTCTGCCACCATCGTTTACTCAAACACCGCCCTGCCCGGCCTGCAAAATCCCTTTGTGGTGGACA
CCAACAGCGAGTTGACCAAGGTGTCAGTGTCGGTTGACCGCGCCACCGGTTTGGCGCTGCGCAGTCATATTGGTTCCAGT
GTGACCGTATCCAGCGAAGGCGGAAAGGACTACGAGTACTACAATGGTACGTCCATGGCGACCCCGCACGTTTCCGGTGT
AGCCACTTTGGTGTGGAGTTATCATCCGGAGTGTAGCGCCGCTCAGGTGCGTGCTGCCCTGCGTACGACTGCCGAAGATC
TGGATGTAGCTGGCCGTGATGACAAGACAGGTTATGGTCTGGTTGATGCAGTTGCCGCCAAAGAGTATCTGGACGCTTCC
TGTGACGGCCCAACCAACGGTGGTGGCAACAATGGCGGCTCAGACGCCGAGATGACCAATGGTGTTGCCAAGAGCAACCT
CGCCGGTGCCAAGAATGAAGAGCTGCACTTCTTCATCGATGTGCCTGCCGGTGCCACCAACCTGAACTTTGCCATGAGCG
GTGGCAGCGGTGATGCGGATCTGTACGTTCAGTACGGTGCGGCTCCAACCACCAGCAGCTATGATTGCCGCCCATGGAAA
GGGGGTAACACTGAGTCTTGCCCAATCACCACCGCACAGGCCGGTACCTACTATGTCATGGTACAGGGTTACAGCGCCTT
CAGTGGTGTGAACCTGGTGGCAAGCTACACTGCCCCCACCGGCGGCAGCACTGGCGGCACCACGGGCCCTGCGACTTACA
CCAACACCGATAACTACAGCATTCCGGACAACAACACCGCCGGGATCAGCAGCCCTATCGATGTAAGCCGCACTGGCGAT
GCGGGCACTGTCACTGTGAAAGTGAACATCGTACACACCTACATTGGCGATTTGCAGGTCGAACTTATCACTCCAAGTGG
TCAGGTAGCCGTGCTGCACGACAACACAGGTGCAGGTACCGATAACATCAACAAGACTTACACTGTGAATGCCACAGGTG
TTGAGTCTCAGGGTACCTGGAAGCTCAAAGCCGTTGACAGCTCACGCCGCGACACTGGCTACATCGACTCATGGGAAATC
TCGTTCCAATAA

Upstream 100 bases:

>100_bases
TCGATGGATACATTCACTGCCGAAAATGGATTTCAGGCTTTACCGGATGGATGCCAGATAACAACGACAAGTTCTGCCTC
TTGCAACATGGAAGACGAGT

Downstream 100 bases:

>100_bases
TCGGTTGAGTCTGTGAAAAAGGAGCCTTGAGGCTCCTTTTTTATTGATGGGATATTCAGTCTGTAAATACCGCAATGTTA
AGATACGCCGTGTTCTGCCG

Product: cold-active serine alkaline protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 803; Mature: 802

Protein sequence:

>803_residues
MTMKHKKTLALGLSTLALAVSAGVHAAPTDAVFAKGENSPLPKRYVVKFKNNAATELYNAEDTLNSMQYQPRSHEVYGHH
RALNAASAKEMKRIGRSNAYTVKLDNNGIKALRARADVEFVEEDVPRRLLAETTPWGQTFVGATQLADNLSGNRTICIID
SGYDRGHADLSGNNVTGTNNSGTGNWFEPGNNNAHGTHVAGTIAAIANGEGVVGVLPNQNANIHIIKVFNEAGWGYSSSL
VSAVDTCVANGANVVSMSLGGASSSTTERNALAAHYNNGVLLIAAAGNDGDNTHSYPASYDSVVSVAAVDSTKQHAAFSQ
YTNQVEVSGPGEAILSTVTRGEGRLSDIVVAGQSYFNNGVVPHNRLVKSGTSYAPAPINGTVTATLAECSVSGSTFNCGN
MAGKVCLVERVGNQGSSYPEINAAKACQNAGASATIVYSNTALPGLQNPFVVDTNSELTKVSVSVDRATGLALRSHIGSS
VTVSSEGGKDYEYYNGTSMATPHVSGVATLVWSYHPECSAAQVRAALRTTAEDLDVAGRDDKTGYGLVDAVAAKEYLDAS
CDGPTNGGGNNGGSDAEMTNGVAKSNLAGAKNEELHFFIDVPAGATNLNFAMSGGSGDADLYVQYGAAPTTSSYDCRPWK
GGNTESCPITTAQAGTYYVMVQGYSAFSGVNLVASYTAPTGGSTGGTTGPATYTNTDNYSIPDNNTAGISSPIDVSRTGD
AGTVTVKVNIVHTYIGDLQVELITPSGQVAVLHDNTGAGTDNINKTYTVNATGVESQGTWKLKAVDSSRRDTGYIDSWEI
SFQ

Sequences:

>Translated_803_residues
MTMKHKKTLALGLSTLALAVSAGVHAAPTDAVFAKGENSPLPKRYVVKFKNNAATELYNAEDTLNSMQYQPRSHEVYGHH
RALNAASAKEMKRIGRSNAYTVKLDNNGIKALRARADVEFVEEDVPRRLLAETTPWGQTFVGATQLADNLSGNRTICIID
SGYDRGHADLSGNNVTGTNNSGTGNWFEPGNNNAHGTHVAGTIAAIANGEGVVGVLPNQNANIHIIKVFNEAGWGYSSSL
VSAVDTCVANGANVVSMSLGGASSSTTERNALAAHYNNGVLLIAAAGNDGDNTHSYPASYDSVVSVAAVDSTKQHAAFSQ
YTNQVEVSGPGEAILSTVTRGEGRLSDIVVAGQSYFNNGVVPHNRLVKSGTSYAPAPINGTVTATLAECSVSGSTFNCGN
MAGKVCLVERVGNQGSSYPEINAAKACQNAGASATIVYSNTALPGLQNPFVVDTNSELTKVSVSVDRATGLALRSHIGSS
VTVSSEGGKDYEYYNGTSMATPHVSGVATLVWSYHPECSAAQVRAALRTTAEDLDVAGRDDKTGYGLVDAVAAKEYLDAS
CDGPTNGGGNNGGSDAEMTNGVAKSNLAGAKNEELHFFIDVPAGATNLNFAMSGGSGDADLYVQYGAAPTTSSYDCRPWK
GGNTESCPITTAQAGTYYVMVQGYSAFSGVNLVASYTAPTGGSTGGTTGPATYTNTDNYSIPDNNTAGISSPIDVSRTGD
AGTVTVKVNIVHTYIGDLQVELITPSGQVAVLHDNTGAGTDNINKTYTVNATGVESQGTWKLKAVDSSRRDTGYIDSWEI
SFQ
>Mature_802_residues
TMKHKKTLALGLSTLALAVSAGVHAAPTDAVFAKGENSPLPKRYVVKFKNNAATELYNAEDTLNSMQYQPRSHEVYGHHR
ALNAASAKEMKRIGRSNAYTVKLDNNGIKALRARADVEFVEEDVPRRLLAETTPWGQTFVGATQLADNLSGNRTICIIDS
GYDRGHADLSGNNVTGTNNSGTGNWFEPGNNNAHGTHVAGTIAAIANGEGVVGVLPNQNANIHIIKVFNEAGWGYSSSLV
SAVDTCVANGANVVSMSLGGASSSTTERNALAAHYNNGVLLIAAAGNDGDNTHSYPASYDSVVSVAAVDSTKQHAAFSQY
TNQVEVSGPGEAILSTVTRGEGRLSDIVVAGQSYFNNGVVPHNRLVKSGTSYAPAPINGTVTATLAECSVSGSTFNCGNM
AGKVCLVERVGNQGSSYPEINAAKACQNAGASATIVYSNTALPGLQNPFVVDTNSELTKVSVSVDRATGLALRSHIGSSV
TVSSEGGKDYEYYNGTSMATPHVSGVATLVWSYHPECSAAQVRAALRTTAEDLDVAGRDDKTGYGLVDAVAAKEYLDASC
DGPTNGGGNNGGSDAEMTNGVAKSNLAGAKNEELHFFIDVPAGATNLNFAMSGGSGDADLYVQYGAAPTTSSYDCRPWKG
GNTESCPITTAQAGTYYVMVQGYSAFSGVNLVASYTAPTGGSTGGTTGPATYTNTDNYSIPDNNTAGISSPIDVSRTGDA
GTVTVKVNIVHTYIGDLQVELITPSGQVAVLHDNTGAGTDNINKTYTVNATGVESQGTWKLKAVDSSRRDTGYIDSWEIS
FQ

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 83454; Mature: 83323

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMKHKKTLALGLSTLALAVSAGVHAAPTDAVFAKGENSPLPKRYVVKFKNNAATELYNA
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCEEECC
EDTLNSMQYQPRSHEVYGHHRALNAASAKEMKRIGRSNAYTVKLDNNGIKALRARADVEF
HHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHCCHHH
VEEDVPRRLLAETTPWGQTFVGATQLADNLSGNRTICIIDSGYDRGHADLSGNNVTGTNN
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCEECCCCC
SGTGNWFEPGNNNAHGTHVAGTIAAIANGEGVVGVLPNQNANIHIIKVFNEAGWGYSSSL
CCCCCCCCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCEEEEEEEECCCCCHHHHH
VSAVDTCVANGANVVSMSLGGASSSTTERNALAAHYNNGVLLIAAAGNDGDNTHSYPASY
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCC
DSVVSVAAVDSTKQHAAFSQYTNQVEVSGPGEAILSTVTRGEGRLSDIVVAGQSYFNNGV
HHEEEEEEECCCHHHHHHHHHCCEEEECCCHHHHHHHHHCCCCCEEEEEEECHHHHCCCC
VPHNRLVKSGTSYAPAPINGTVTATLAECSVSGSTFNCGNMAGKVCLVERVGNQGSSYPE
CCCHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCEEECCCCCCCEEEEEECCCCCCCCCC
INAAKACQNAGASATIVYSNTALPGLQNPFVVDTNSELTKVSVSVDRATGLALRSHIGSS
CCHHHHHHCCCCEEEEEEECCCCCCCCCCEEEECCCCEEEEEEEECHHHHHHHHHHCCCE
VTVSSEGGKDYEYYNGTSMATPHVSGVATLVWSYHPECSAAQVRAALRTTAEDLDVAGRD
EEEECCCCCCCEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCHHHCCCCCCC
DKTGYGLVDAVAAKEYLDASCDGPTNGGGNNGGSDAEMTNGVAKSNLAGAKNEELHFFID
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCHHCCCCCCCCCCEEEEEE
VPAGATNLNFAMSGGSGDADLYVQYGAAPTTSSYDCRPWKGGNTESCPITTAQAGTYYVM
CCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEE
VQGYSAFSGVNLVASYTAPTGGSTGGTTGPATYTNTDNYSIPDNNTAGISSPIDVSRTGD
EECCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC
AGTVTVKVNIVHTYIGDLQVELITPSGQVAVLHDNTGAGTDNINKTYTVNATGVESQGTW
CEEEEEEEEEEEEEECCEEEEEECCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCE
KLKAVDSSRRDTGYIDSWEISFQ
EEEEECCCCCCCCCEECEEEEEC
>Mature Secondary Structure 
TMKHKKTLALGLSTLALAVSAGVHAAPTDAVFAKGENSPLPKRYVVKFKNNAATELYNA
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCEEECC
EDTLNSMQYQPRSHEVYGHHRALNAASAKEMKRIGRSNAYTVKLDNNGIKALRARADVEF
HHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHCCHHH
VEEDVPRRLLAETTPWGQTFVGATQLADNLSGNRTICIIDSGYDRGHADLSGNNVTGTNN
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCEECCCCC
SGTGNWFEPGNNNAHGTHVAGTIAAIANGEGVVGVLPNQNANIHIIKVFNEAGWGYSSSL
CCCCCCCCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCEEEEEEEECCCCCHHHHH
VSAVDTCVANGANVVSMSLGGASSSTTERNALAAHYNNGVLLIAAAGNDGDNTHSYPASY
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCC
DSVVSVAAVDSTKQHAAFSQYTNQVEVSGPGEAILSTVTRGEGRLSDIVVAGQSYFNNGV
HHEEEEEEECCCHHHHHHHHHCCEEEECCCHHHHHHHHHCCCCCEEEEEEECHHHHCCCC
VPHNRLVKSGTSYAPAPINGTVTATLAECSVSGSTFNCGNMAGKVCLVERVGNQGSSYPE
CCCHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCEEECCCCCCCEEEEEECCCCCCCCCC
INAAKACQNAGASATIVYSNTALPGLQNPFVVDTNSELTKVSVSVDRATGLALRSHIGSS
CCHHHHHHCCCCEEEEEEECCCCCCCCCCEEEECCCCEEEEEEEECHHHHHHHHHHCCCE
VTVSSEGGKDYEYYNGTSMATPHVSGVATLVWSYHPECSAAQVRAALRTTAEDLDVAGRD
EEEECCCCCCCEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCHHHCCCCCCC
DKTGYGLVDAVAAKEYLDASCDGPTNGGGNNGGSDAEMTNGVAKSNLAGAKNEELHFFID
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCHHCCCCCCCCCCEEEEEE
VPAGATNLNFAMSGGSGDADLYVQYGAAPTTSSYDCRPWKGGNTESCPITTAQAGTYYVM
CCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEE
VQGYSAFSGVNLVASYTAPTGGSTGGTTGPATYTNTDNYSIPDNNTAGISSPIDVSRTGD
EECCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC
AGTVTVKVNIVHTYIGDLQVELITPSGQVAVLHDNTGAGTDNINKTYTVNATGVESQGTW
CEEEEEEEEEEEEEECCEEEEEECCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCE
KLKAVDSSRRDTGYIDSWEISFQ
EEEEECCCCCCCCCEECEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3001653; 4967581; 8512925; 9425066 [H]