Click here to switch to the map view.

The map label for this gene is pdxB

Identifier: 119775288

GI number: 119775288

Start: 2601632

End: 2602759

Strand: Reverse

Name: pdxB

Synonym: Sama_2153

Alternate gene names: 119775288

Gene position: 2602759-2601632 (Counterclockwise)

Preceding gene: 119775289

Following gene: 119775287

Centisome position: 60.44

GC content: 52.84

Gene sequence:

>1128_bases
ATGAAAATTATCGCGGACGAGAATATGCCCTATGTGGATGTACTCTTCGGAGAGCTTGGGGAGATTGAATACGTCAATGG
CCGCACTCTGGCACCTGAGCAGTTAGCCGATGCCGATGTGCTGCTGGTACGCTCGGTAACTCAGGTCAATGAAGCACTAT
TGTGCCAAAACCAACAGCTTAAGTTTGTTGGCAGCGCCACCATAGGGACAGACCATGTGGACACAGCCTATCTGAAAAGC
CGGGGTATCCCCTTTACCAATGCGCCCGGGTGCAATGCCACTGCAGTAGGTGAATATGCGTTTATTGCCATGCTGGAGCT
TGCTCAGCGTTATCGGCAGCCGCTGAAAGATAAAAAAGTCGCGGTAATAGGTGCCGGGAACACTGGCACTGCCACAGCCC
GTTGTCTCGAAGCATATGGGGTCGAACACCGGCTGTGCGACCCCGTGTTGGCAGCACAGGGCGACAATCGCCAATTTTAC
GAGCTCGATGAGCTTATTGCCTGGGCCGATGTGATAAGCCTGCATGTGCCAATTACCAAAGGTGGCGATCATCCCACCTG
GTATTTGTTCGACCAACAACGACTTGAAGCCCTGAAAACCGGTGCCTGGCTGCTCAACTGCTGCCGTGGTGAGGTGATAG
ACAATCGAGCCCTTATCGAAGTTAAGCAAAGACGGGTCGACATCAAACTGGTGTTGGATGTGTGGGAAGGCGAGCCCAGT
CCCATGTTGGCGCTGGTGCCGCTGGTGGATATCGCCACGCCACACATTGCCGGTTATAGCCTGGAAGGTAAGGCCAGAGG
CACTTTTATGCTGTATGAAGCGCTTTGCCGGGTAATGGGTAAGACTGGTGAAAATAGCTTCCACAGCCTGCTGCCCCCCT
TCTTTTTGGGCTCAATGCAAGTTCGGGAGTTGGGGGACGAGAGGGCACTGCTGACACTGTGCCGGACGGTATACGATCTG
CGGGATGACGATATCCACTTCAGGCAATCGGGCACCGACAGACTCGGATTTGACAAAATGAGAAAAAATCACCGTCACAG
ACGCGAATTCAGCGCCCTTAAGCTGGAAAATGCTAAGGGATCTGAGGTAAATTGGTTGTCCTTTTTGGGTTTTTCTAACG
TCGGTTGA

Upstream 100 bases:

>100_bases
GCGACCACAGTTTTCGCAAAATCAAAAGGGAAGCCAGGCTTCCCTTTTTTATTGTCTGTTACAATCCGCCCCAATCGCCA
AAGAAGGAAAGCCGACAGAG

Downstream 100 bases:

>100_bases
GTATTATGTCGCAGGAATTTAATGTCGTAGTTTTGGGTGCATCGGGCGCCGTGGGTCAGACCATGATCGAGATCCTCGAA
GAGCGCAATTTCCCGGTTGC

Product: erythronate-4-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKIIADENMPYVDVLFGELGEIEYVNGRTLAPEQLADADVLLVRSVTQVNEALLCQNQQLKFVGSATIGTDHVDTAYLKS
RGIPFTNAPGCNATAVGEYAFIAMLELAQRYRQPLKDKKVAVIGAGNTGTATARCLEAYGVEHRLCDPVLAAQGDNRQFY
ELDELIAWADVISLHVPITKGGDHPTWYLFDQQRLEALKTGAWLLNCCRGEVIDNRALIEVKQRRVDIKLVLDVWEGEPS
PMLALVPLVDIATPHIAGYSLEGKARGTFMLYEALCRVMGKTGENSFHSLLPPFFLGSMQVRELGDERALLTLCRTVYDL
RDDDIHFRQSGTDRLGFDKMRKNHRHRREFSALKLENAKGSEVNWLSFLGFSNVG

Sequences:

>Translated_375_residues
MKIIADENMPYVDVLFGELGEIEYVNGRTLAPEQLADADVLLVRSVTQVNEALLCQNQQLKFVGSATIGTDHVDTAYLKS
RGIPFTNAPGCNATAVGEYAFIAMLELAQRYRQPLKDKKVAVIGAGNTGTATARCLEAYGVEHRLCDPVLAAQGDNRQFY
ELDELIAWADVISLHVPITKGGDHPTWYLFDQQRLEALKTGAWLLNCCRGEVIDNRALIEVKQRRVDIKLVLDVWEGEPS
PMLALVPLVDIATPHIAGYSLEGKARGTFMLYEALCRVMGKTGENSFHSLLPPFFLGSMQVRELGDERALLTLCRTVYDL
RDDDIHFRQSGTDRLGFDKMRKNHRHRREFSALKLENAKGSEVNWLSFLGFSNVG
>Mature_375_residues
MKIIADENMPYVDVLFGELGEIEYVNGRTLAPEQLADADVLLVRSVTQVNEALLCQNQQLKFVGSATIGTDHVDTAYLKS
RGIPFTNAPGCNATAVGEYAFIAMLELAQRYRQPLKDKKVAVIGAGNTGTATARCLEAYGVEHRLCDPVLAAQGDNRQFY
ELDELIAWADVISLHVPITKGGDHPTWYLFDQQRLEALKTGAWLLNCCRGEVIDNRALIEVKQRRVDIKLVLDVWEGEPS
PMLALVPLVDIATPHIAGYSLEGKARGTFMLYEALCRVMGKTGENSFHSLLPPFFLGSMQVRELGDERALLTLCRTVYDL
RDDDIHFRQSGTDRLGFDKMRKNHRHRREFSALKLENAKGSEVNWLSFLGFSNVG

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily

Homologues:

Organism=Homo sapiens, GI23308577, Length=273, Percent_Identity=25.6410256410256, Blast_Score=85, Evalue=9e-17,
Organism=Escherichia coli, GI1788660, Length=374, Percent_Identity=46.524064171123, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI1789279, Length=266, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787645, Length=199, Percent_Identity=28.643216080402, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI87082289, Length=257, Percent_Identity=26.0700389105058, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=256, Percent_Identity=27.734375, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6322116, Length=331, Percent_Identity=27.7945619335347, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6320925, Length=367, Percent_Identity=27.2479564032698, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324964, Length=280, Percent_Identity=22.8571428571429, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19921140, Length=283, Percent_Identity=23.6749116607774, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28574284, Length=243, Percent_Identity=25.1028806584362, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45551003, Length=243, Percent_Identity=25.1028806584362, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585514, Length=243, Percent_Identity=25.1028806584362, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574282, Length=243, Percent_Identity=25.1028806584362, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45552429, Length=243, Percent_Identity=25.1028806584362, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXB_SHEAM (A1S7K2)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928028.1
- ProteinModelPortal:   A1S7K2
- SMR:   A1S7K2
- STRING:   A1S7K2
- GeneID:   4604403
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2153
- NMPDR:   fig|326297.7.peg.2021
- eggNOG:   COG0111
- HOGENOM:   HBG289972
- OMA:   SAPGCNA
- PhylomeDB:   A1S7K2
- ProtClustDB:   CLSK906984
- GO:   GO:0005737
- HAMAP:   MF_01825
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10996:SF4

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: =1.1.1.290

Molecular weight: Translated: 41860; Mature: 41860

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 209-209 ACT_SITE 238-238 ACT_SITE 255-255 BINDING 45-45 BINDING 67-67 BINDING 147-147 BINDING 233-233 BINDING 258-258 BINDING 259-259

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIADENMPYVDVLFGELGEIEYVNGRTLAPEQLADADVLLVRSVTQVNEALLCQNQQL
CEEEECCCCCCHHHHHCCCCCEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCE
KFVGSATIGTDHVDTAYLKSRGIPFTNAPGCNATAVGEYAFIAMLELAQRYRQPLKDKKV
EEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCE
AVIGAGNTGTATARCLEAYGVEHRLCDPVLAAQGDNRQFYELDELIAWADVISLHVPITK
EEEECCCCCHHHHHHHHHHCCCHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEEEC
GGDHPTWYLFDQQRLEALKTGAWLLNCCRGEVIDNRALIEVKQRRVDIKLVLDVWEGEPS
CCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEEEEEEEECCCCC
PMLALVPLVDIATPHIAGYSLEGKARGTFMLYEALCRVMGKTGENSFHSLLPPFFLGSMQ
CEEEEEEHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCHHHHCCHH
VRELGDERALLTLCRTVYDLRDDDIHFRQSGTDRLGFDKMRKNHRHRREFSALKLENAKG
HHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHEEECCCCC
SEVNWLSFLGFSNVG
CCCEEEEECCCCCCC
>Mature Secondary Structure
MKIIADENMPYVDVLFGELGEIEYVNGRTLAPEQLADADVLLVRSVTQVNEALLCQNQQL
CEEEECCCCCCHHHHHCCCCCEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCE
KFVGSATIGTDHVDTAYLKSRGIPFTNAPGCNATAVGEYAFIAMLELAQRYRQPLKDKKV
EEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCE
AVIGAGNTGTATARCLEAYGVEHRLCDPVLAAQGDNRQFYELDELIAWADVISLHVPITK
EEEECCCCCHHHHHHHHHHCCCHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEEEC
GGDHPTWYLFDQQRLEALKTGAWLLNCCRGEVIDNRALIEVKQRRVDIKLVLDVWEGEPS
CCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEEEEEEEECCCCC
PMLALVPLVDIATPHIAGYSLEGKARGTFMLYEALCRVMGKTGENSFHSLLPPFFLGSMQ
CEEEEEEHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCHHHHCCHH
VRELGDERALLTLCRTVYDLRDDDIHFRQSGTDRLGFDKMRKNHRHRREFSALKLENAKG
HHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHEEECCCCC
SEVNWLSFLGFSNVG
CCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA