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The map label for this gene is xthA [H]

Identifier: 119775274

GI number: 119775274

Start: 2584035

End: 2584841

Strand: Direct

Name: xthA [H]

Synonym: Sama_2139

Alternate gene names: 119775274

Gene position: 2584035-2584841 (Clockwise)

Preceding gene: 119775273

Following gene: 119775278

Centisome position: 60.01

GC content: 53.66

Gene sequence:

>807_bases
ATGAAGGTCGTTTCATTTAACATCAATGGCCTGCGATCGCGCCTGCATCAACTTCAAGCGCTGATTGATACCCAGGCGCC
TGATATCATTGGCCTGCAGGAAACCAAGGTGCACGACGAAGCCTTTCCCCTCGCCGATGTCGAAGCCATGGGTTACAAGG
TGTATTTCCACGGGGGCAAGGCCCATTATGGGGTGGCGCTGCTGTCGAAGGTTGAACCTGAATCCGTGGAAAAGGGTTAC
CCATGGGATGCAGAAGATGCCCAAAGGCGGATGATCATCGGTAAATTCCGCCAGCCAAATGGCCGCTTGCTTACCGTGAT
GAACGGCTACTTCCCCCAGGGTGAAAGCCGGGATCACGAAATCAAGTTTCCCGCCAAGCGTAAGTTTTATGCCGATCTCC
AGCGACTCCTGACCGAATCCTACAGTTGCGATGACGACATCATCATCATGGGGGATATCAACATCTCGCCACTGGATTTG
GACATAGGCATTGGTCCGGATAATGCCAAACGCTGGCTCAGAACCGGTAAATGCAGTTTCCTGCCGGAAGAGCGCGAGTG
GCTGCAAACCCTGAAAGACTGGGGATTTGACGACAGCTTCCGGGTACTGCACCCGGAAAAAGACGCTACCTATTCCTGGT
TCGATTACCGCAGTAAGGGCTTTGATGATAACCGCGGGCTGCGTATCGATGTGATCATGACAACAAGGTCACTCACAGGC
AGGCTGGTTGCGTCCGGTGTGGATTACAATATTCGCGCCATGGAAAAGCCGTCGGATCACGCCCCCGTCTGGAGCGAGTT
CGAGTAA

Upstream 100 bases:

>100_bases
CACTGGAACTCGCCCAGGCCTGGGCCGATGCCGATCCTTACATTGCCGCTGGGGTGTATGAAAAGGTGATCGTAAAGCCC
TTTAAGCGAGTGTTGCCCTG

Downstream 100 bases:

>100_bases
GCTGGCTGTTGGTCATAAAAAAGCGCCGATAACCGGCGCTTTTTTTATGTCTCATGCTGAATTTTCGTCAGCCATCAACG
ACTGAGGCTCGTTCGAGATA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKVVSFNINGLRSRLHQLQALIDTQAPDIIGLQETKVHDEAFPLADVEAMGYKVYFHGGKAHYGVALLSKVEPESVEKGY
PWDAEDAQRRMIIGKFRQPNGRLLTVMNGYFPQGESRDHEIKFPAKRKFYADLQRLLTESYSCDDDIIIMGDINISPLDL
DIGIGPDNAKRWLRTGKCSFLPEEREWLQTLKDWGFDDSFRVLHPEKDATYSWFDYRSKGFDDNRGLRIDVIMTTRSLTG
RLVASGVDYNIRAMEKPSDHAPVWSEFE

Sequences:

>Translated_268_residues
MKVVSFNINGLRSRLHQLQALIDTQAPDIIGLQETKVHDEAFPLADVEAMGYKVYFHGGKAHYGVALLSKVEPESVEKGY
PWDAEDAQRRMIIGKFRQPNGRLLTVMNGYFPQGESRDHEIKFPAKRKFYADLQRLLTESYSCDDDIIIMGDINISPLDL
DIGIGPDNAKRWLRTGKCSFLPEEREWLQTLKDWGFDDSFRVLHPEKDATYSWFDYRSKGFDDNRGLRIDVIMTTRSLTG
RLVASGVDYNIRAMEKPSDHAPVWSEFE
>Mature_268_residues
MKVVSFNINGLRSRLHQLQALIDTQAPDIIGLQETKVHDEAFPLADVEAMGYKVYFHGGKAHYGVALLSKVEPESVEKGY
PWDAEDAQRRMIIGKFRQPNGRLLTVMNGYFPQGESRDHEIKFPAKRKFYADLQRLLTESYSCDDDIIIMGDINISPLDL
DIGIGPDNAKRWLRTGKCSFLPEEREWLQTLKDWGFDDSFRVLHPEKDATYSWFDYRSKGFDDNRGLRIDVIMTTRSLTG
RLVASGVDYNIRAMEKPSDHAPVWSEFE

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=270, Percent_Identity=24.8148148148148, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI18375503, Length=270, Percent_Identity=24.8148148148148, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI18375501, Length=270, Percent_Identity=24.8148148148148, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=61.0486891385768, Blast_Score=352, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI71989536, Length=271, Percent_Identity=25.830258302583, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI221330655, Length=272, Percent_Identity=30.8823529411765, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17136678, Length=272, Percent_Identity=30.8823529411765, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30781; Mature: 30781

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVSFNINGLRSRLHQLQALIDTQAPDIIGLQETKVHDEAFPLADVEAMGYKVYFHGGK
CEEEEECHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCHHHCCEEEEEECCC
AHYGVALLSKVEPESVEKGYPWDAEDAQRRMIIGKFRQPNGRLLTVMNGYFPQGESRDHE
CCCHHHHHHCCCHHHHCCCCCCCHHHHHHEEEEEEEECCCCCEEEEEECCCCCCCCCCCE
IKFPAKRKFYADLQRLLTESYSCDDDIIIMGDINISPLDLDIGIGPDNAKRWLRTGKCSF
EECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHCCCCCC
LPEEREWLQTLKDWGFDDSFRVLHPEKDATYSWFDYRSKGFDDNRGLRIDVIMTTRSLTG
CCCHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEHHCCCCCCCCCCEEEEEEEECCCCCC
RLVASGVDYNIRAMEKPSDHAPVWSEFE
EEEECCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKVVSFNINGLRSRLHQLQALIDTQAPDIIGLQETKVHDEAFPLADVEAMGYKVYFHGGK
CEEEEECHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCHHHCCEEEEEECCC
AHYGVALLSKVEPESVEKGYPWDAEDAQRRMIIGKFRQPNGRLLTVMNGYFPQGESRDHE
CCCHHHHHHCCCHHHHCCCCCCCHHHHHHEEEEEEEECCCCCEEEEEECCCCCCCCCCCE
IKFPAKRKFYADLQRLLTESYSCDDDIIIMGDINISPLDLDIGIGPDNAKRWLRTGKCSF
EECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHCCCCCC
LPEEREWLQTLKDWGFDDSFRVLHPEKDATYSWFDYRSKGFDDNRGLRIDVIMTTRSLTG
CCCHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEHHCCCCCCCCCCEEEEEEEECCCCCC
RLVASGVDYNIRAMEKPSDHAPVWSEFE
EEEECCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]