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The map label for this gene is rluB [H]

Identifier: 119775259

GI number: 119775259

Start: 2569814

End: 2570701

Strand: Reverse

Name: rluB [H]

Synonym: Sama_2124

Alternate gene names: 119775259

Gene position: 2570701-2569814 (Counterclockwise)

Preceding gene: 119775260

Following gene: 119775258

Centisome position: 59.7

GC content: 57.88

Gene sequence:

>888_bases
ATGAGTGAAAAGTTGCAGAAAGTCTTGGCCCGTGCAGGCCATGGCTCCCGTCGTGAGATGGAGGCATGGATTGCTGCAGG
ACGAGTGAGTATCAACGGCGAAATCGCCAACCTCGGAGACCGGGTAGATGCCGACGCCAAAGTGCGTCTCGACGGTCGCC
TGGTGTCTATCAAGTCAGCCGACGATATCATCTGTCGTGTGCTGGCTTACCATAAGCCAGAGGGTGAAATTTGCTCCCGC
AAAGACCCGGAAGGCCGTCCCACGGTATTTGACCGCCTGCCCAAGCTGCGTGACGCCCGTTGGGTGGCAGTGGGTCGTCT
GGATATCAACACTTCGGGCCTGTTGCTGTTTACCTCTGACGGTGAACTGGCTAACCGCCTGATGCACCCGTCCAACGAAG
TTGACCGTGAGTATGCTGTGCGTACCTTTGGTGAAGTGAATGACTTTACCATTCAAAAGCTGCGTACCGGCGTGATGCTT
GAAGACGGTCCGGCGCAGTTTGATTATGTAAAGGCTGCCGGTGGTGAAGGTGTCAACCAATGGTGGCACGTGGGACTGCG
CGAAGGGCGTAACCGTGAAGTGCGCCGCCTGTGGGAATCCCAGGAAGTGCAGGTGAGCCGTCTTATCCGCGTGCGTTACG
GTATGATTGAACTGCCGAAAACCTTGCCCCGTGGTGGCTGGATTGAACTGCCGCTGGAGCAGGTAAACTACCTGCGTCAG
CTGGCTGGTATGGATCCTGAAACCCGCTCCATGATTGGTACCGACAAGCACAGTGTGGCGCGTGCGTCCCAGGTGCGCAG
CGCCAAAATTCGCCGCGCAGTGCGCAAGAGCAAGGTAGGCGCCGCCACCGGCTCAAAACGTCCCAAGCCCACTCGCACCC
GCAGTTAA

Upstream 100 bases:

>100_bases
GATAATCTGGCGTCATTACCGGCGCTGGCGGATCTGGAGTCATTGGATGCGTTATTTGGCAAACAAACTGAATTACCAAA
TGCGCAGGAAGAATTAACTC

Downstream 100 bases:

>100_bases
TCCCGCCTAAGGCGTTGGATATCTGAGTAAAAAAGGCCGCTTATGCGGCCTTTTGTGTTATACCAATCAGTAAATCAGAC
TGTTTACATGGAAGCGACAT

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSEKLQKVLARAGHGSRREMEAWIAAGRVSINGEIANLGDRVDADAKVRLDGRLVSIKSADDIICRVLAYHKPEGEICSR
KDPEGRPTVFDRLPKLRDARWVAVGRLDINTSGLLLFTSDGELANRLMHPSNEVDREYAVRTFGEVNDFTIQKLRTGVML
EDGPAQFDYVKAAGGEGVNQWWHVGLREGRNREVRRLWESQEVQVSRLIRVRYGMIELPKTLPRGGWIELPLEQVNYLRQ
LAGMDPETRSMIGTDKHSVARASQVRSAKIRRAVRKSKVGAATGSKRPKPTRTRS

Sequences:

>Translated_295_residues
MSEKLQKVLARAGHGSRREMEAWIAAGRVSINGEIANLGDRVDADAKVRLDGRLVSIKSADDIICRVLAYHKPEGEICSR
KDPEGRPTVFDRLPKLRDARWVAVGRLDINTSGLLLFTSDGELANRLMHPSNEVDREYAVRTFGEVNDFTIQKLRTGVML
EDGPAQFDYVKAAGGEGVNQWWHVGLREGRNREVRRLWESQEVQVSRLIRVRYGMIELPKTLPRGGWIELPLEQVNYLRQ
LAGMDPETRSMIGTDKHSVARASQVRSAKIRRAVRKSKVGAATGSKRPKPTRTRS
>Mature_294_residues
SEKLQKVLARAGHGSRREMEAWIAAGRVSINGEIANLGDRVDADAKVRLDGRLVSIKSADDIICRVLAYHKPEGEICSRK
DPEGRPTVFDRLPKLRDARWVAVGRLDINTSGLLLFTSDGELANRLMHPSNEVDREYAVRTFGEVNDFTIQKLRTGVMLE
DGPAQFDYVKAAGGEGVNQWWHVGLREGRNREVRRLWESQEVQVSRLIRVRYGMIELPKTLPRGGWIELPLEQVNYLRQL
AGMDPETRSMIGTDKHSVARASQVRSAKIRRAVRKSKVGAATGSKRPKPTRTRS

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=278, Percent_Identity=66.1870503597122, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1790453, Length=240, Percent_Identity=28.3333333333333, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=29.9145299145299, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081838, Length=186, Percent_Identity=31.1827956989247, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 33202; Mature: 33071

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKLQKVLARAGHGSRREMEAWIAAGRVSINGEIANLGDRVDADAKVRLDGRLVSIKSA
CCHHHHHHHHHCCCCCHHHHHHHHHHCEEEECCCHHCCCCCCCCCCEEEECCEEEEECCH
DDIICRVLAYHKPEGEICSRKDPEGRPTVFDRLPKLRDARWVAVGRLDINTSGLLLFTSD
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCHHCCCEEEEEEEEEECCCEEEEEECC
GELANRLMHPSNEVDREYAVRTFGEVNDFTIQKLRTGVMLEDGPAQFDYVKAAGGEGVNQ
CHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCEECCCCCCEEEEECCCCCCHHH
WWHVGLREGRNREVRRLWESQEVQVSRLIRVRYGMIELPKTLPRGGWIELPLEQVNYLRQ
HHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHCCCCCEEECCHHHHHHHHH
LAGMDPETRSMIGTDKHSVARASQVRSAKIRRAVRKSKVGAATGSKRPKPTRTRS
HHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEKLQKVLARAGHGSRREMEAWIAAGRVSINGEIANLGDRVDADAKVRLDGRLVSIKSA
CHHHHHHHHHCCCCCHHHHHHHHHHCEEEECCCHHCCCCCCCCCCEEEECCEEEEECCH
DDIICRVLAYHKPEGEICSRKDPEGRPTVFDRLPKLRDARWVAVGRLDINTSGLLLFTSD
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCHHCCCEEEEEEEEEECCCEEEEEECC
GELANRLMHPSNEVDREYAVRTFGEVNDFTIQKLRTGVMLEDGPAQFDYVKAAGGEGVNQ
CHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCEECCCCCCEEEEECCCCCCHHH
WWHVGLREGRNREVRRLWESQEVQVSRLIRVRYGMIELPKTLPRGGWIELPLEQVNYLRQ
HHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHCCCCCEEECCHHHHHHHHH
LAGMDPETRSMIGTDKHSVARASQVRSAKIRRAVRKSKVGAATGSKRPKPTRTRS
HHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]