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The map label for this gene is asnB [H]

Identifier: 119775236

GI number: 119775236

Start: 2546210

End: 2547874

Strand: Direct

Name: asnB [H]

Synonym: Sama_2101

Alternate gene names: 119775236

Gene position: 2546210-2547874 (Clockwise)

Preceding gene: 119775235

Following gene: 119775244

Centisome position: 59.13

GC content: 56.1

Gene sequence:

>1665_bases
ATGTGTTCGATATTTGCCATTCTCGATATCCAGTCTGATGCCAGCGCCATGCGCCAGGTTGCCCTGGAAATGTCCAAATT
ATTGCGTCACCGCGGCCCCGACTGGTCAGGTATTTACGCCGACGACCACGCTGTGCTGGCCCACGAGCGCCTTGCAATTG
TTGACATAGAGCACGGTGCCCAGCCGCTTATCAGTGACGATGGCAGCCTGGTACTGGCAGTGAATGGGGAAATCTACAAC
CACAAAGAACTCAAGGCAGAGCTTGGCGACAAGTACAGTTACCAGACCAACAGCGACTGCGAAGTGATCCTGGCACTGTA
CCAGGAATACGGCAGCGACTTTCTCGACAAGCTCAACGGCATCTTCGCATTTGTACTGTACGACAAAGCCAAGGGCACGT
ATCTGATTGGCCGCGACCATATTGGCATCATTCCACTCTACACCGGCCGTGACGCCAAGGGTAACTTCTATGTGGCGTCC
GAAATGAAGGCATTGGTGCCGGTTTGTAAAACCGTCGAAACCTTTGCCCCCGGTCATTATCTGTCAAGCACCGACGCGCA
GCCCGTTCGTTACTACCAGCGCGACTGGCAAGCTTTTGACGCTGTGGTCGACAACGAGGCCAGCGTGGAAGAACTGCGCG
GTGCACTCGAAGCCGCCGTGAAGCGCCAGTTGATGTCAGATGTGCCCTATGGTGTGTTGCTGTCCGGCGGTTTGGACTCA
TCCATCGTGTCGGCCATCACCCAAACCTTTGCCAAGCGTCGTATTGAAGACAACGATGAAAGTGAAGCCTGGTGGCCCCA
GCTGCACTCCTTTGCCGTGGGTCTCAAAGGCTCGCCCGACTTGGCCGCATCACGCAAGGTAGCCGATGCCATTGGCACCA
TACACCATGAAATCCACTTTACCTTCCAGGAAGGGCTGGATGCCATCAAGGACGTGATTTATCACCTGGAGACCTATGAT
GTCACCACCATACGCGCGGCCACACCTATGTACCTGATGGCCCGTAAAATCAAGGCTATGGGTATCAAGATGGTCCTGTC
TGGCGAAGGCGCTGATGAGCTGTTTGGCGGTTACCTGTATTTCCATAAGGCGCCTAACCCTCAGGCGTTCCACGAAGAGC
TGGTGCGTAAACTCGACAAGCTGCACCTGTTCGATTGCCTGCGCGCCAACAAGGCCATGGCAGCCTGGGGCCTGGAAGCG
CGTGTGCCTTTCCTCGATAAAGAATTTATGGATGTGGCGATGCGCTTAAACCCGGCCGCCAAGATGTCCGGTAAGGGCAA
AATCGAGAAGCACATTCTGCGGGAAGCCTTCGAACACAAGTTACCGGCCGAAATCGCCTGGCGTCAGAAAGAACAGTTCA
GCGATGGCGTAGGTTACAGCTGGATTGATGGTCTCAAGGAGCAAGCCGCGGCTCAGGTGGACGATTTGCAGCTGGCCAAT
GCCCGTTTCCGCTTCCCGCACAACACGCCGGAAACCAAGGAGGCCTATTTCTACCGCTGCTTCTTTGAAGAGTTGTTCCC
GCTGGAGTCTGCGGCCCTTACCGTACCGGGTGGCAAGAGTGTGGCCTGCTCGACGCCTGAGGCGCTTGCCTGGGATGCCA
GCCTGCAGGGTATTATCGACCCCTCCGGCCGAGCAGTGCGGGAAGTACACGCCCAAAGCTACTGA

Upstream 100 bases:

>100_bases
AAAAAATCACATTTCATCATTTTTATTGTTTGCTTATTGCAGAATTCACTGGCAAAGTGACAAAACATTGATGCCTATGA
AGTGACGTGCGGAGTTTGTT

Downstream 100 bases:

>100_bases
AGCCGCTACCCCATGGTTCGCTTTTCCGCGAGAGCGGATAAAAACACCTCAGCCTAATAACCAAAAAGCCCCGCACTGAA
GACGGGGCTTTTACTTGTGA

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MCSIFAILDIQSDASAMRQVALEMSKLLRHRGPDWSGIYADDHAVLAHERLAIVDIEHGAQPLISDDGSLVLAVNGEIYN
HKELKAELGDKYSYQTNSDCEVILALYQEYGSDFLDKLNGIFAFVLYDKAKGTYLIGRDHIGIIPLYTGRDAKGNFYVAS
EMKALVPVCKTVETFAPGHYLSSTDAQPVRYYQRDWQAFDAVVDNEASVEELRGALEAAVKRQLMSDVPYGVLLSGGLDS
SIVSAITQTFAKRRIEDNDESEAWWPQLHSFAVGLKGSPDLAASRKVADAIGTIHHEIHFTFQEGLDAIKDVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNPQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRLNPAAKMSGKGKIEKHILREAFEHKLPAEIAWRQKEQFSDGVGYSWIDGLKEQAAAQVDDLQLAN
ARFRFPHNTPETKEAYFYRCFFEELFPLESAALTVPGGKSVACSTPEALAWDASLQGIIDPSGRAVREVHAQSY

Sequences:

>Translated_554_residues
MCSIFAILDIQSDASAMRQVALEMSKLLRHRGPDWSGIYADDHAVLAHERLAIVDIEHGAQPLISDDGSLVLAVNGEIYN
HKELKAELGDKYSYQTNSDCEVILALYQEYGSDFLDKLNGIFAFVLYDKAKGTYLIGRDHIGIIPLYTGRDAKGNFYVAS
EMKALVPVCKTVETFAPGHYLSSTDAQPVRYYQRDWQAFDAVVDNEASVEELRGALEAAVKRQLMSDVPYGVLLSGGLDS
SIVSAITQTFAKRRIEDNDESEAWWPQLHSFAVGLKGSPDLAASRKVADAIGTIHHEIHFTFQEGLDAIKDVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNPQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRLNPAAKMSGKGKIEKHILREAFEHKLPAEIAWRQKEQFSDGVGYSWIDGLKEQAAAQVDDLQLAN
ARFRFPHNTPETKEAYFYRCFFEELFPLESAALTVPGGKSVACSTPEALAWDASLQGIIDPSGRAVREVHAQSY
>Mature_554_residues
MCSIFAILDIQSDASAMRQVALEMSKLLRHRGPDWSGIYADDHAVLAHERLAIVDIEHGAQPLISDDGSLVLAVNGEIYN
HKELKAELGDKYSYQTNSDCEVILALYQEYGSDFLDKLNGIFAFVLYDKAKGTYLIGRDHIGIIPLYTGRDAKGNFYVAS
EMKALVPVCKTVETFAPGHYLSSTDAQPVRYYQRDWQAFDAVVDNEASVEELRGALEAAVKRQLMSDVPYGVLLSGGLDS
SIVSAITQTFAKRRIEDNDESEAWWPQLHSFAVGLKGSPDLAASRKVADAIGTIHHEIHFTFQEGLDAIKDVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNPQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRLNPAAKMSGKGKIEKHILREAFEHKLPAEIAWRQKEQFSDGVGYSWIDGLKEQAAAQVDDLQLAN
ARFRFPHNTPETKEAYFYRCFFEELFPLESAALTVPGGKSVACSTPEALAWDASLQGIIDPSGRAVREVHAQSY

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=544, Percent_Identity=41.5441176470588, Blast_Score=349, Evalue=4e-96,
Organism=Homo sapiens, GI168229252, Length=544, Percent_Identity=41.5441176470588, Blast_Score=349, Evalue=4e-96,
Organism=Homo sapiens, GI168229250, Length=544, Percent_Identity=41.5441176470588, Blast_Score=349, Evalue=4e-96,
Organism=Homo sapiens, GI296010848, Length=515, Percent_Identity=42.7184466019417, Blast_Score=346, Evalue=4e-95,
Organism=Homo sapiens, GI296010852, Length=450, Percent_Identity=43.7777777777778, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI296010850, Length=450, Percent_Identity=43.7777777777778, Blast_Score=313, Evalue=2e-85,
Organism=Escherichia coli, GI1786889, Length=554, Percent_Identity=70.0361010830325, Blast_Score=820, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=561, Percent_Identity=43.8502673796791, Blast_Score=436, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71993933, Length=559, Percent_Identity=43.649373881932, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17560178, Length=521, Percent_Identity=36.6602687140115, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI25147560, Length=311, Percent_Identity=38.9067524115756, Blast_Score=197, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321563, Length=545, Percent_Identity=50.6422018348624, Blast_Score=532, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6325403, Length=546, Percent_Identity=49.6336996336996, Blast_Score=522, Evalue=1e-149,
Organism=Drosophila melanogaster, GI45553209, Length=570, Percent_Identity=47.1929824561404, Blast_Score=504, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 61801; Mature: 61801

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIFAILDIQSDASAMRQVALEMSKLLRHRGPDWSGIYADDHAVLAHERLAIVDIEHGA
CCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEECCEEEEEECCCC
QPLISDDGSLVLAVNGEIYNHKELKAELGDKYSYQTNSDCEVILALYQEYGSDFLDKLNG
CCCCCCCCCEEEEECCCEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHH
IFAFVLYDKAKGTYLIGRDHIGIIPLYTGRDAKGNFYVASEMKALVPVCKTVETFAPGHY
EEEEEEEECCCCCEEEECCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
LSSTDAQPVRYYQRDWQAFDAVVDNEASVEELRGALEAAVKRQLMSDVPYGVLLSGGLDS
CCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SIVSAITQTFAKRRIEDNDESEAWWPQLHSFAVGLKGSPDLAASRKVADAIGTIHHEIHF
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHEEE
TFQEGLDAIKDVIYHLETYDVTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLY
EHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNPQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEARVPFLDKEFMDVAMRLNPAA
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCHH
KMSGKGKIEKHILREAFEHKLPAEIAWRQKEQFSDGVGYSWIDGLKEQAAAQVDDLQLAN
HCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECC
ARFRFPHNTPETKEAYFYRCFFEELFPLESAALTVPGGKSVACSTPEALAWDASLQGIID
CEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCEECCCCCCEEECCCCCCCCC
PSGRAVREVHAQSY
CCCHHHHHHHHCCC
>Mature Secondary Structure
MCSIFAILDIQSDASAMRQVALEMSKLLRHRGPDWSGIYADDHAVLAHERLAIVDIEHGA
CCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEECCEEEEEECCCC
QPLISDDGSLVLAVNGEIYNHKELKAELGDKYSYQTNSDCEVILALYQEYGSDFLDKLNG
CCCCCCCCCEEEEECCCEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHH
IFAFVLYDKAKGTYLIGRDHIGIIPLYTGRDAKGNFYVASEMKALVPVCKTVETFAPGHY
EEEEEEEECCCCCEEEECCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
LSSTDAQPVRYYQRDWQAFDAVVDNEASVEELRGALEAAVKRQLMSDVPYGVLLSGGLDS
CCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SIVSAITQTFAKRRIEDNDESEAWWPQLHSFAVGLKGSPDLAASRKVADAIGTIHHEIHF
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHEEE
TFQEGLDAIKDVIYHLETYDVTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLY
EHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNPQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEARVPFLDKEFMDVAMRLNPAA
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCHH
KMSGKGKIEKHILREAFEHKLPAEIAWRQKEQFSDGVGYSWIDGLKEQAAAQVDDLQLAN
HCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECC
ARFRFPHNTPETKEAYFYRCFFEELFPLESAALTVPGGKSVACSTPEALAWDASLQGIID
CEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCEECCCCCCEEECCCCCCCCC
PSGRAVREVHAQSY
CCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]