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The map label for this gene is pheS

Identifier: 119775088

GI number: 119775088

Start: 2381027

End: 2382010

Strand: Reverse

Name: pheS

Synonym: Sama_1953

Alternate gene names: 119775088

Gene position: 2382010-2381027 (Counterclockwise)

Preceding gene: 119775091

Following gene: 119775087

Centisome position: 55.32

GC content: 50.81

Gene sequence:

>984_bases
ATGCAGCAATTAACAGAGATTGTGGAACAGGCTCTGGCCGCCATCGAACAGGCGACAGATCTGAAGGTTCTGGACGATAT
CCGGGTTGATTATCTCGGTAAAAAAGGCCAGATCACCGACCTAATGAAACTGATGGGCACCCTGAGTGCAGAAGAGAAGC
CCAAGTTCGGTCAGGCAGTAAACGATGCCAAACAGAGCGTTCAGGCCAAACTCGGTGAGCAGATGGAGCTGTTTAAAGCC
CGCGAACTGGAAGCCAAACTGGCCGCAGAGCAAATCGACGTGACTCTGCCAGGGCGTACTCTGGATAATGGCGGCCTGCA
CCCAGTGACCCGCACCATTGAACGTATTGAATCTTTCTTTGGCGAGCTTGGTTTTACCGTTAAGCAAGGTCCAGAAATCG
AAGATGACTTCCACAACTTCGATGCGCTGAACATTTCTGAACATCATCCGGCCCGTGCCGACCACGATACCTTTTATTTC
AATCCCAAGGTGATGTTGCGTACCCAAACCTCGGGCGTGCAAATTCGCACCATGGAAACGGAAAAACCACCGCTGCGGAT
TATCTCTCCCGGCCGTGTGTACCGTAACGACTACGACATGACCCATACCCCGATGTTCCATCAGGTGGAAGGTCTCTTGG
TGGATGAAAACGTTAACTTTGCAGAGCTTAAAGGCATTCTGCATGATTTCCTGCGTAACTTTTTTGAAGAAGACCTGCAG
GTACGTTTCCGTCCGTCTTACTTCCCATTTACCGAACCTTCAGCCGAAGTGGACGTAATGGGCAAAAATGGCAAGTGGCT
CGAAGTGCTCGGTTGCGGCATGGTACACCCCAACGTACTGCGCAGCGTGGGTATCGACCCTGAGAAGTATTCCGGTTTTG
CTTTTGGTATGGGTGTTGAGCGTTTGACCATGCTGCGCTATGGCGTAAACGATCTGCGCGCATTCTTCGAAAACGACCTG
CGTTTCCTCAAGCAATTCAAATAA

Upstream 100 bases:

>100_bases
CACTCTTTTGTTTATTTTGACAAATTTACGCGGCAAGCACGTCGACATGTCGTGAACAGCCTGCCCAAAAAGGTCAGGCA
CGAGAGCAGTGGAAGAGAAG

Downstream 100 bases:

>100_bases
CGGAGCAAATTAGTAATGAAATTCAGTGAATCCTGGCTTCGTGAATGGGTTAATCCAGCCATCAGCCGTGAAGACTTAAG
CCATCAAATCACCATGGCAG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MQQLTEIVEQALAAIEQATDLKVLDDIRVDYLGKKGQITDLMKLMGTLSAEEKPKFGQAVNDAKQSVQAKLGEQMELFKA
RELEAKLAAEQIDVTLPGRTLDNGGLHPVTRTIERIESFFGELGFTVKQGPEIEDDFHNFDALNISEHHPARADHDTFYF
NPKVMLRTQTSGVQIRTMETEKPPLRIISPGRVYRNDYDMTHTPMFHQVEGLLVDENVNFAELKGILHDFLRNFFEEDLQ
VRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLTMLRYGVNDLRAFFENDL
RFLKQFK

Sequences:

>Translated_327_residues
MQQLTEIVEQALAAIEQATDLKVLDDIRVDYLGKKGQITDLMKLMGTLSAEEKPKFGQAVNDAKQSVQAKLGEQMELFKA
RELEAKLAAEQIDVTLPGRTLDNGGLHPVTRTIERIESFFGELGFTVKQGPEIEDDFHNFDALNISEHHPARADHDTFYF
NPKVMLRTQTSGVQIRTMETEKPPLRIISPGRVYRNDYDMTHTPMFHQVEGLLVDENVNFAELKGILHDFLRNFFEEDLQ
VRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLTMLRYGVNDLRAFFENDL
RFLKQFK
>Mature_327_residues
MQQLTEIVEQALAAIEQATDLKVLDDIRVDYLGKKGQITDLMKLMGTLSAEEKPKFGQAVNDAKQSVQAKLGEQMELFKA
RELEAKLAAEQIDVTLPGRTLDNGGLHPVTRTIERIESFFGELGFTVKQGPEIEDDFHNFDALNISEHHPARADHDTFYF
NPKVMLRTQTSGVQIRTMETEKPPLRIISPGRVYRNDYDMTHTPMFHQVEGLLVDENVNFAELKGILHDFLRNFFEEDLQ
VRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLTMLRYGVNDLRAFFENDL
RFLKQFK

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=259, Percent_Identity=33.5907335907336, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI5729820, Length=278, Percent_Identity=32.7338129496403, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1788007, Length=327, Percent_Identity=73.0886850152905, Blast_Score=516, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI32566635, Length=245, Percent_Identity=35.1020408163265, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17508957, Length=265, Percent_Identity=30.5660377358491, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32563657, Length=265, Percent_Identity=30.5660377358491, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321087, Length=366, Percent_Identity=28.6885245901639, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6325304, Length=152, Percent_Identity=33.5526315789474, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI18858079, Length=258, Percent_Identity=31.3953488372093, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17137424, Length=280, Percent_Identity=28.5714285714286, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_SHEAM (A1S702)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927828.1
- ProteinModelPortal:   A1S702
- SMR:   A1S702
- STRING:   A1S702
- GeneID:   4604203
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1953
- NMPDR:   fig|326297.7.peg.1835
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   A1S702
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 37322; Mature: 37322

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQLTEIVEQALAAIEQATDLKVLDDIRVDYLGKKGQITDLMKLMGTLSAEEKPKFGQAV
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
NDAKQSVQAKLGEQMELFKARELEAKLAAEQIDVTLPGRTLDNGGLHPVTRTIERIESFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHH
GELGFTVKQGPEIEDDFHNFDALNISEHHPARADHDTFYFNPKVMLRTQTSGVQIRTMET
HHHCCEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEECCEEEEEEECCCEEEEEECC
EKPPLRIISPGRVYRNDYDMTHTPMFHQVEGLLVDENVNFAELKGILHDFLRNFFEEDLQ
CCCCEEEECCCCEECCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHE
VRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVE
EEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCHHHCCCEEECCCHH
RLTMLRYGVNDLRAFFENDLRFLKQFK
HHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQQLTEIVEQALAAIEQATDLKVLDDIRVDYLGKKGQITDLMKLMGTLSAEEKPKFGQAV
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
NDAKQSVQAKLGEQMELFKARELEAKLAAEQIDVTLPGRTLDNGGLHPVTRTIERIESFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHH
GELGFTVKQGPEIEDDFHNFDALNISEHHPARADHDTFYFNPKVMLRTQTSGVQIRTMET
HHHCCEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEECCEEEEEEECCCEEEEEECC
EKPPLRIISPGRVYRNDYDMTHTPMFHQVEGLLVDENVNFAELKGILHDFLRNFFEEDLQ
CCCCEEEECCCCEECCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHE
VRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVE
EEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCHHHCCCEEECCCHH
RLTMLRYGVNDLRAFFENDLRFLKQFK
HHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA