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The map label for this gene is aprN [H]

Identifier: 119775011

GI number: 119775011

Start: 2291278

End: 2293101

Strand: Direct

Name: aprN [H]

Synonym: Sama_1876

Alternate gene names: 119775011

Gene position: 2291278-2293101 (Clockwise)

Preceding gene: 119775010

Following gene: 119775012

Centisome position: 53.21

GC content: 52.41

Gene sequence:

>1824_bases
ATGAAAACAGTGAATAAAACCCTATTAGCACTGGGTATTGGTATGGCCATGGCCGCCAATGCCGCCGATGACAGATACGT
TATTCAGGTAGATGCCGGGCATAAAGGCGTGGTGAAGGCCCTCGCCAAACAACTGGGCGGTAAAATTCACGCCGAGGGCA
ATCGCTTTTTCTCAGCCACCTTCAGCGGCAAAGATCTTGCGACCGTAAAAGGGTTATTGAACAACCCACACATTCAACTC
ATCGAAGAAGATGCCAAACGGGAACTGATGGGCTACAGCGACGATGTGGGCGATCCGCGCAATACACAAATCACCCCTTA
TGCAGTCTATCAATCTCAGGCTAATCAACTCACGCTCCAGGCAAACTCAGGCGTCAAAGTCTGTGTAATTGACTCAGGGC
TCGATCGTTCAAACCCCGACTTCCTATGGCAAAACATCACGGGTGACAACGACCCCGGCACCGGCAATTGGGATCAAAAT
GGTGGCCCCCACGGCACCCACGTTGCCGGCACCATAGGTGCAGCCGATAACGGCTTTGGCGTGGTTGGCATGGCGCCGGG
TGTCGACATGCACATCATCAAGGTATTTAACGCCAATGGCTGGGGCTATTCATCCGATCTTGCACTTGCCACCGAGAAGT
GTGCTGCAGCAGGGGCAAACATCATCAATATGAGTCTGGGTGGTGGACGTCCCAACAGTACAGAAGAGAATGCCTTCAAG
GAGTTCACCAAAAATGGCGGCCTGGTGATTGCAGCAGCAGGTAACGACGGTAACAACGTCAGAACCTACCCGGCTGGCTA
TGAGTCAGTCATGATGATTGGCGCCAACAATGCCAATGATGCAATCGCGGACTTTTCCCAGTTTCCTGCCTGCACCATTA
TCTACTCAGGTAAGGGCAACAAGAATAATCAGCAGATTATTGATACCACCTGCGTGGAAGTCACCGCAGGGGGCGTTGAT
ACCCTGTCCACCTACCCTGCCGATATGGCGGTAGCGTCGGTATTAAACGCTGATGGTCAGGTACTTGGCTCTACCGGAAT
GGGCTACGCTGAACAAGGCAATGTCACCGGCAGCGGCTATTACATGGGCCTTGGCAAGGCAATAGATTCAGGCGCCGACG
GAAAGGTCTGCCTGATTGATAGAGGCGAAATATCTTTTAATGACAAAGTCATGAATTGCCAAGCCTCCGGTGGTATTGCT
GCCGTTATCATCAATAACGTTGCGGGTATGCTGTATGGCACCTTGGGCGCCGACACCACAACCGGTATTCCGGCCGTTGG
CGCCACACTGGAAGACCGCAGTGCTATCCTGGCTGCAACCTCAGTTGGCGTAGAGATCAGTAGCTCAGATTATGGCTTTA
TGAGCGGAACCTCCATGGCGACCCCAGCCGTGAGTGGTTTGGCAGCCTTATTGTGGTCGAACCATCCAGAATGCAGTGGC
AATGACATTCGCGCAGCGCTTAAAGCAACAGCCTTTGATGCCGGCGCCACCGGGAGGGATGACTACTTTGGTTATGGCAT
CGTCAAAGTGGCCGACGCCCATCAATACCTGTTGAGCAACCACTGCAGTGCCAAACCCATGGTAGACATCCAACTCACCA
CGGCTACGTACTCAGGCAAACGCGGAGAACAGGTTGAACTTAATTGGTCGGGCGCCTCAACCCGCAAGGTCAATGTGTTT
CGTAATGGCGTGGTAATTACCGCCACTGCCAACGATGGTAACTTTGTTGATACGCTCAACAACCCTTATGGCATCTACAC
TTACCAAATCTGTGAAGATGGCACCGCGAACTGCTCTGACATTTCAAGCGTGACATTCAACTAA

Upstream 100 bases:

>100_bases
ATTTCAGGAAATCGCAGTGTCAGAGCAACAGCCGATGGAATTGACTACAAATGCGTTGCGCAACTGGCCTGCTATTTACC
AACAACTTTCAGGAACACAA

Downstream 100 bases:

>100_bases
CTGCTAAGCCGTATTAAAAGAGCGCCTGAAGGCGCTCTTTTTGTTAATGATACAACGTTATGAATCGCCACTGATTTCCT
AGACACCTTTGAGTTAGATA

Product: peptidase S8/S53 subtilisin kexin sedolisin

Products: NA

Alternate protein names: Nattokinase [H]

Number of amino acids: Translated: 607; Mature: 607

Protein sequence:

>607_residues
MKTVNKTLLALGIGMAMAANAADDRYVIQVDAGHKGVVKALAKQLGGKIHAEGNRFFSATFSGKDLATVKGLLNNPHIQL
IEEDAKRELMGYSDDVGDPRNTQITPYAVYQSQANQLTLQANSGVKVCVIDSGLDRSNPDFLWQNITGDNDPGTGNWDQN
GGPHGTHVAGTIGAADNGFGVVGMAPGVDMHIIKVFNANGWGYSSDLALATEKCAAAGANIINMSLGGGRPNSTEENAFK
EFTKNGGLVIAAAGNDGNNVRTYPAGYESVMMIGANNANDAIADFSQFPACTIIYSGKGNKNNQQIIDTTCVEVTAGGVD
TLSTYPADMAVASVLNADGQVLGSTGMGYAEQGNVTGSGYYMGLGKAIDSGADGKVCLIDRGEISFNDKVMNCQASGGIA
AVIINNVAGMLYGTLGADTTTGIPAVGATLEDRSAILAATSVGVEISSSDYGFMSGTSMATPAVSGLAALLWSNHPECSG
NDIRAALKATAFDAGATGRDDYFGYGIVKVADAHQYLLSNHCSAKPMVDIQLTTATYSGKRGEQVELNWSGASTRKVNVF
RNGVVITATANDGNFVDTLNNPYGIYTYQICEDGTANCSDISSVTFN

Sequences:

>Translated_607_residues
MKTVNKTLLALGIGMAMAANAADDRYVIQVDAGHKGVVKALAKQLGGKIHAEGNRFFSATFSGKDLATVKGLLNNPHIQL
IEEDAKRELMGYSDDVGDPRNTQITPYAVYQSQANQLTLQANSGVKVCVIDSGLDRSNPDFLWQNITGDNDPGTGNWDQN
GGPHGTHVAGTIGAADNGFGVVGMAPGVDMHIIKVFNANGWGYSSDLALATEKCAAAGANIINMSLGGGRPNSTEENAFK
EFTKNGGLVIAAAGNDGNNVRTYPAGYESVMMIGANNANDAIADFSQFPACTIIYSGKGNKNNQQIIDTTCVEVTAGGVD
TLSTYPADMAVASVLNADGQVLGSTGMGYAEQGNVTGSGYYMGLGKAIDSGADGKVCLIDRGEISFNDKVMNCQASGGIA
AVIINNVAGMLYGTLGADTTTGIPAVGATLEDRSAILAATSVGVEISSSDYGFMSGTSMATPAVSGLAALLWSNHPECSG
NDIRAALKATAFDAGATGRDDYFGYGIVKVADAHQYLLSNHCSAKPMVDIQLTTATYSGKRGEQVELNWSGASTRKVNVF
RNGVVITATANDGNFVDTLNNPYGIYTYQICEDGTANCSDISSVTFN
>Mature_607_residues
MKTVNKTLLALGIGMAMAANAADDRYVIQVDAGHKGVVKALAKQLGGKIHAEGNRFFSATFSGKDLATVKGLLNNPHIQL
IEEDAKRELMGYSDDVGDPRNTQITPYAVYQSQANQLTLQANSGVKVCVIDSGLDRSNPDFLWQNITGDNDPGTGNWDQN
GGPHGTHVAGTIGAADNGFGVVGMAPGVDMHIIKVFNANGWGYSSDLALATEKCAAAGANIINMSLGGGRPNSTEENAFK
EFTKNGGLVIAAAGNDGNNVRTYPAGYESVMMIGANNANDAIADFSQFPACTIIYSGKGNKNNQQIIDTTCVEVTAGGVD
TLSTYPADMAVASVLNADGQVLGSTGMGYAEQGNVTGSGYYMGLGKAIDSGADGKVCLIDRGEISFNDKVMNCQASGGIA
AVIINNVAGMLYGTLGADTTTGIPAVGATLEDRSAILAATSVGVEISSSDYGFMSGTSMATPAVSGLAALLWSNHPECSG
NDIRAALKATAFDAGATGRDDYFGYGIVKVADAHQYLLSNHCSAKPMVDIQLTTATYSGKRGEQVELNWSGASTRKVNVF
RNGVVITATANDGNFVDTLNNPYGIYTYQICEDGTANCSDISSVTFN

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Drosophila melanogaster, GI45550681, Length=174, Percent_Identity=31.6091954022989, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 63051; Mature: 63051

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVNKTLLALGIGMAMAANAADDRYVIQVDAGHKGVVKALAKQLGGKIHAEGNRFFSAT
CCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCEEEECCCEEEEEE
FSGKDLATVKGLLNNPHIQLIEEDAKRELMGYSDDVGDPRNTQITPYAVYQSQANQLTLQ
ECCCCHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCEEEEE
ANSGVKVCVIDSGLDRSNPDFLWQNITGDNDPGTGNWDQNGGPHGTHVAGTIGAADNGFG
ECCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEE
VVGMAPGVDMHIIKVFNANGWGYSSDLALATEKCAAAGANIINMSLGGGRPNSTEENAFK
EEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
EFTKNGGLVIAAAGNDGNNVRTYPAGYESVMMIGANNANDAIADFSQFPACTIIYSGKGN
HHHCCCCEEEEEECCCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHCCEEEEEECCCCC
KNNQQIIDTTCVEVTAGGVDTLSTYPADMAVASVLNADGQVLGSTGMGYAEQGNVTGSGY
CCCCEEEEEEEEEEECCCCCHHHCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCEE
YMGLGKAIDSGADGKVCLIDRGEISFNDKVMNCQASGGIAAVIINNVAGMLYGTLGADTT
EEEECHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEEEHHHHHHEEEECCCCCC
TGIPAVGATLEDRSAILAATSVGVEISSSDYGFMSGTSMATPAVSGLAALLWSNHPECSG
CCCCCCCCCCCCCCEEEEEEEECEEECCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCC
NDIRAALKATAFDAGATGRDDYFGYGIVKVADAHQYLLSNHCSAKPMVDIQLTTATYSGK
CHHHHEEEEEEECCCCCCCCCCCCEEEEEEECHHHHHHHCCCCCCCEEEEEEEEEEECCC
RGEQVELNWSGASTRKVNVFRNGVVITATANDGNFVDTLNNPYGIYTYQICEDGTANCSD
CCCEEEEEECCCCCEEEEEEECCEEEEEECCCCCEEECCCCCCEEEEEEEECCCCCCCCC
ISSVTFN
CCEEECC
>Mature Secondary Structure
MKTVNKTLLALGIGMAMAANAADDRYVIQVDAGHKGVVKALAKQLGGKIHAEGNRFFSAT
CCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCEEEECCCEEEEEE
FSGKDLATVKGLLNNPHIQLIEEDAKRELMGYSDDVGDPRNTQITPYAVYQSQANQLTLQ
ECCCCHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCEEEEE
ANSGVKVCVIDSGLDRSNPDFLWQNITGDNDPGTGNWDQNGGPHGTHVAGTIGAADNGFG
ECCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEE
VVGMAPGVDMHIIKVFNANGWGYSSDLALATEKCAAAGANIINMSLGGGRPNSTEENAFK
EEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
EFTKNGGLVIAAAGNDGNNVRTYPAGYESVMMIGANNANDAIADFSQFPACTIIYSGKGN
HHHCCCCEEEEEECCCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHCCEEEEEECCCCC
KNNQQIIDTTCVEVTAGGVDTLSTYPADMAVASVLNADGQVLGSTGMGYAEQGNVTGSGY
CCCCEEEEEEEEEEECCCCCHHHCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCEE
YMGLGKAIDSGADGKVCLIDRGEISFNDKVMNCQASGGIAAVIINNVAGMLYGTLGADTT
EEEECHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEEEHHHHHHEEEECCCCCC
TGIPAVGATLEDRSAILAATSVGVEISSSDYGFMSGTSMATPAVSGLAALLWSNHPECSG
CCCCCCCCCCCCCCEEEEEEEECEEECCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCC
NDIRAALKATAFDAGATGRDDYFGYGIVKVADAHQYLLSNHCSAKPMVDIQLTTATYSGK
CHHHHEEEEEEECCCCCCCCCCCCEEEEEEECHHHHHHHCCCCCCCEEEEEEEEEEECCC
RGEQVELNWSGASTRKVNVFRNGVVITATANDGNFVDTLNNPYGIYTYQICEDGTANCSD
CCCEEEEEECCCCCEEEEEEECCEEEEEECCCCCEEECCCCCCEEEEEEEECCCCCCCCC
ISSVTFN
CCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1369081 [H]