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The map label for this gene is aspS

Identifier: 119774978

GI number: 119774978

Start: 2243378

End: 2245153

Strand: Reverse

Name: aspS

Synonym: Sama_1843

Alternate gene names: 119774978

Gene position: 2245153-2243378 (Counterclockwise)

Preceding gene: 119774979

Following gene: 119774977

Centisome position: 52.14

GC content: 56.53

Gene sequence:

>1776_bases
ATGCGCAGTCATTATTGTGGAGACGTTAATAAGTCTCACGTCGGACAAGAAGTCACCCTCGTTGGCTGGGTAAACCGCAG
CCGCGATTTGGGGGGTGTGATCTTTTTGGATCTTCGCGACCGCGAAGGTTTGGTTCAGGTAGTATATGACCCGGACTTGC
CAGACGTGTTTGACGTAGCCAGCAGCCTGCGTGCCGAGTTCTGTGTTCAGGTGAAAGGTGTGGTTCGCCCACGTCCTGAC
AGCCAGGTGAACAGCCAGATGAAAACCGGTGAAATTGAGGTGCTGGGTAAAGCGCTCACCATCATCAATGCCGCCGACCC
GCTGCCACTGAGCCTGGATAACCACCAGAACAACAGCGAAGAAGCCCGTCTGAAGTACCGCTATCTGGATCTGCGCCGTC
CTGAAATGGCACAGCGTCTGATTTTCCGTGCCAAGGTCACCAGCTTTGTGCGTCGCTTTATGGATGGCAACGGCTTCCTC
GACATTGAAACCCCTATCCTGACCAAGGCCACTCCGGAAGGTGCCCGCGACTATCTGGTGCCAAGCCGTACCTATAAAGG
TCAGTTCTTCGCACTGCCACAGTCGCCACAGCTGTTCAAACAGCTGCTGATGATGTCAGGTTTTGACCGTTACTATCAAA
TCGTCAAGTGTTTCCGCGACGAAGACCTGCGCGCCGACCGTCAGCCTGAATTCACCCAAATCGATATCGAAACCTCGTTC
ATGACCTCAGATCAGGTGATGGAAACCACCGAGCGTATGATCCGTAACCTGTTCCTCGAGCTGATGAACGTGGATCTGGG
TGATTTCCCGAAAATGACCTGGGATGAAGCCATGCGCCGCTTTGGCTCCGACAAACCTGATCTACGTAACCCGCTGGAAC
TGGTTGATGTAGCCGATCTGCTTAAAGCCGTTGAGTTTGCGGTATTCTCCGGCCCTGCCAACGATGAAGAAGGCCGCGTT
GCTGCCCTGCGTATCCCCGGCGGCGCCGAGCTGTCACGCAAGCAAATCGACGACTACACCAAGTTTGTAGGAATCTACGG
TGCCCGCGGTCTGGCCTGGATGAAGGTCAACAACCTGGCTGCTGGCGTTGAAGGCATTCAGTCACCTGTGGCTAAGTTCC
TGAATGAAGACATCATCAAGGAAATCATTGCCCGCACCAAGGCTGCCGATGGCGATATCATCTTCTTCGGTGCTGACAAG
GCCAACGTGGTTGCCGAGTCCATGGGCGCCCTGCGTCTGAAAGCCGGCGAAGACTTCAAACTGCTCGAAGGTGAGTGGCG
CCCACTGTGGGTGGTTGACTTCCCGATGTTCGAAAAGGCCGATGGCCGTTTCTACGCAGTTCACCATCCCTTTACCGCGC
CGCGCGGCGTCACTGCTGCCGAGCTTGAAGCGAGCCCAGGCAAGGCCGTGTCCGATGCCTACGACATGGTACTCAACGGC
GTTGAGCTGGGCGGCGGCTCTGTGCGTATCCACAATGGCGACATGCAGTCTACCGTGTTCCGTATCCTTGGCATCGACGA
TGAAGAAGCCAAAGAAAAGTTCGGCTTCCTGCTGGACGCACTGCGTTTCGGTACCCCACCACACGCGGGTCTGGCCTTTG
GTCTGGACCGTCTGGTGATGCTGATGACCGGCGCCAGCTCCATCCGTGATGTGATGGCCTTCCCCAAGACCACCACGGCG
GCCTGTCCGCTCACCAACGCGCCGGGACATGCCAACCCCGACCAACTGGTGGAACTGGGCATTGCGGTTCTGCCGAAAGA
GCCGAAACAAGACTGA

Upstream 100 bases:

>100_bases
GTGAAGTACTTTGGCTCAGGGCATTATTTCTCTATAATGCCCAGTCACAATTTTCAATCAGATTTCCGCCACACCGGTGC
AGGAAAAAGGATAACTCTCA

Downstream 100 bases:

>100_bases
TAGAGTCACAAACAAACCGGGGCGCATGCCTCGGTCTGTTTTTACGGCCACTGCATTTTCCCCGGACGCTGTTTTTCGTT
AGTCTGGACACTGGTCATCT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVIFLDLRDREGLVQVVYDPDLPDVFDVASSLRAEFCVQVKGVVRPRPD
SQVNSQMKTGEIEVLGKALTIINAADPLPLSLDNHQNNSEEARLKYRYLDLRRPEMAQRLIFRAKVTSFVRRFMDGNGFL
DIETPILTKATPEGARDYLVPSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
MTSDQVMETTERMIRNLFLELMNVDLGDFPKMTWDEAMRRFGSDKPDLRNPLELVDVADLLKAVEFAVFSGPANDEEGRV
AALRIPGGAELSRKQIDDYTKFVGIYGARGLAWMKVNNLAAGVEGIQSPVAKFLNEDIIKEIIARTKAADGDIIFFGADK
ANVVAESMGALRLKAGEDFKLLEGEWRPLWVVDFPMFEKADGRFYAVHHPFTAPRGVTAAELEASPGKAVSDAYDMVLNG
VELGGGSVRIHNGDMQSTVFRILGIDDEEAKEKFGFLLDALRFGTPPHAGLAFGLDRLVMLMTGASSIRDVMAFPKTTTA
ACPLTNAPGHANPDQLVELGIAVLPKEPKQD

Sequences:

>Translated_591_residues
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVIFLDLRDREGLVQVVYDPDLPDVFDVASSLRAEFCVQVKGVVRPRPD
SQVNSQMKTGEIEVLGKALTIINAADPLPLSLDNHQNNSEEARLKYRYLDLRRPEMAQRLIFRAKVTSFVRRFMDGNGFL
DIETPILTKATPEGARDYLVPSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
MTSDQVMETTERMIRNLFLELMNVDLGDFPKMTWDEAMRRFGSDKPDLRNPLELVDVADLLKAVEFAVFSGPANDEEGRV
AALRIPGGAELSRKQIDDYTKFVGIYGARGLAWMKVNNLAAGVEGIQSPVAKFLNEDIIKEIIARTKAADGDIIFFGADK
ANVVAESMGALRLKAGEDFKLLEGEWRPLWVVDFPMFEKADGRFYAVHHPFTAPRGVTAAELEASPGKAVSDAYDMVLNG
VELGGGSVRIHNGDMQSTVFRILGIDDEEAKEKFGFLLDALRFGTPPHAGLAFGLDRLVMLMTGASSIRDVMAFPKTTTA
ACPLTNAPGHANPDQLVELGIAVLPKEPKQD
>Mature_591_residues
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVIFLDLRDREGLVQVVYDPDLPDVFDVASSLRAEFCVQVKGVVRPRPD
SQVNSQMKTGEIEVLGKALTIINAADPLPLSLDNHQNNSEEARLKYRYLDLRRPEMAQRLIFRAKVTSFVRRFMDGNGFL
DIETPILTKATPEGARDYLVPSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
MTSDQVMETTERMIRNLFLELMNVDLGDFPKMTWDEAMRRFGSDKPDLRNPLELVDVADLLKAVEFAVFSGPANDEEGRV
AALRIPGGAELSRKQIDDYTKFVGIYGARGLAWMKVNNLAAGVEGIQSPVAKFLNEDIIKEIIARTKAADGDIIFFGADK
ANVVAESMGALRLKAGEDFKLLEGEWRPLWVVDFPMFEKADGRFYAVHHPFTAPRGVTAAELEASPGKAVSDAYDMVLNG
VELGGGSVRIHNGDMQSTVFRILGIDDEEAKEKFGFLLDALRFGTPPHAGLAFGLDRLVMLMTGASSIRDVMAFPKTTTA
ACPLTNAPGHANPDQLVELGIAVLPKEPKQD

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=604, Percent_Identity=39.2384105960265, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI5031815, Length=252, Percent_Identity=27.3809523809524, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI194272210, Length=252, Percent_Identity=27.3809523809524, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI45439306, Length=268, Percent_Identity=27.9850746268657, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI226491502, Length=253, Percent_Identity=26.4822134387352, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1788173, Length=588, Percent_Identity=70.7482993197279, Blast_Score=866, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=300, Percent_Identity=28, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790571, Length=294, Percent_Identity=26.8707482993197, Blast_Score=90, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=600, Percent_Identity=34, Blast_Score=323, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI71984122, Length=252, Percent_Identity=28.1746031746032, Blast_Score=86, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71994340, Length=180, Percent_Identity=27.2222222222222, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17535927, Length=180, Percent_Identity=27.2222222222222, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=180, Percent_Identity=27.2222222222222, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=275, Percent_Identity=26.9090909090909, Blast_Score=83, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6325153, Length=625, Percent_Identity=33.28, Blast_Score=281, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6323011, Length=268, Percent_Identity=29.1044776119403, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321807, Length=252, Percent_Identity=28.1746031746032, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324256, Length=178, Percent_Identity=25.2808988764045, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=173, Percent_Identity=25.4335260115607, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24584738, Length=611, Percent_Identity=35.1882160392799, Blast_Score=339, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24640849, Length=253, Percent_Identity=27.2727272727273, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24640851, Length=253, Percent_Identity=27.2727272727273, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17136276, Length=274, Percent_Identity=28.8321167883212, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_SHEAM (A1S6P2)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927718.1
- ProteinModelPortal:   A1S6P2
- SMR:   A1S6P2
- STRING:   A1S6P2
- GeneID:   4604093
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1843
- NMPDR:   fig|326297.7.peg.1734
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- PhylomeDB:   A1S6P2
- ProtClustDB:   PRK00476
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 65826; Mature: 65826

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVIFLDLRDREGLVQVVYDPDLPDVFDVA
CCCCCCCCCCHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHH
SSLRAEFCVQVKGVVRPRPDSQVNSQMKTGEIEVLGKALTIINAADPLPLSLDNHQNNSE
HHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHEEEECCCCCCEEECCCCCCCH
EARLKYRYLDLRRPEMAQRLIFRAKVTSFVRRFMDGNGFLDIETPILTKATPEGARDYLV
HHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCCCC
PSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
CCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEC
MTSDQVMETTERMIRNLFLELMNVDLGDFPKMTWDEAMRRFGSDKPDLRNPLELVDVADL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHH
LKAVEFAVFSGPANDEEGRVAALRIPGGAELSRKQIDDYTKFVGIYGARGLAWMKVNNLA
HHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHH
AGVEGIQSPVAKFLNEDIIKEIIARTKAADGDIIFFGADKANVVAESMGALRLKAGEDFK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHCCCCEEEECCCCCE
LLEGEWRPLWVVDFPMFEKADGRFYAVHHPFTAPRGVTAAELEASPGKAVSDAYDMVLNG
EECCCCCEEEEEECCCEECCCCCEEEEECCCCCCCCCCCEECCCCCCCHHHHHHHHHHCC
VELGGGSVRIHNGDMQSTVFRILGIDDEEAKEKFGFLLDALRFGTPPHAGLAFGLDRLVM
EEECCCEEEEECCCHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LMTGASSIRDVMAFPKTTTAACPLTNAPGHANPDQLVELGIAVLPKEPKQD
HHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVIFLDLRDREGLVQVVYDPDLPDVFDVA
CCCCCCCCCCHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHH
SSLRAEFCVQVKGVVRPRPDSQVNSQMKTGEIEVLGKALTIINAADPLPLSLDNHQNNSE
HHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHEEEECCCCCCEEECCCCCCCH
EARLKYRYLDLRRPEMAQRLIFRAKVTSFVRRFMDGNGFLDIETPILTKATPEGARDYLV
HHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCCCC
PSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
CCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEC
MTSDQVMETTERMIRNLFLELMNVDLGDFPKMTWDEAMRRFGSDKPDLRNPLELVDVADL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHH
LKAVEFAVFSGPANDEEGRVAALRIPGGAELSRKQIDDYTKFVGIYGARGLAWMKVNNLA
HHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHH
AGVEGIQSPVAKFLNEDIIKEIIARTKAADGDIIFFGADKANVVAESMGALRLKAGEDFK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHCCCCEEEECCCCCE
LLEGEWRPLWVVDFPMFEKADGRFYAVHHPFTAPRGVTAAELEASPGKAVSDAYDMVLNG
EECCCCCEEEEEECCCEECCCCCEEEEECCCCCCCCCCCEECCCCCCCHHHHHHHHHHCC
VELGGGSVRIHNGDMQSTVFRILGIDDEEAKEKFGFLLDALRFGTPPHAGLAFGLDRLVM
EEECCCEEEEECCCHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LMTGASSIRDVMAFPKTTTAACPLTNAPGHANPDQLVELGIAVLPKEPKQD
HHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA