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The map label for this gene is ptsI [H]

Identifier: 119774786

GI number: 119774786

Start: 2014505

End: 2016208

Strand: Reverse

Name: ptsI [H]

Synonym: Sama_1650

Alternate gene names: 119774786

Gene position: 2016208-2014505 (Counterclockwise)

Preceding gene: 119774787

Following gene: 119774785

Centisome position: 46.82

GC content: 57.81

Gene sequence:

>1704_bases
ATGCAGGTTAACGGAATAGCCATCAAACCCGGTGTCACCTTCGGTGAAGCGCTCCACTTCAATACCGGACACAAGCCGCT
TGACTACCGCATCCTGCCCATCAAGCGCATTCCCCAGCAGCTCAGTCGCCTCGATGGTGGATTGGCCAGACTGAAGCAAC
AGCTGAGCGCCAGCCTGCAGGCGCTGGATAGCCAGAGCGATGCCTATGCACTGGTGGAAGCCGACCTCCTGTATCTGGAC
GACCCGGATCTTCGCGCTCACATTGCCGACACCATCACCCAACTGCAATTTTCGGCCTGCGTCTCTATCGAACGTGTTTT
TGCGCATCAGGCGAGTGAACTGGAAGCACTGGACGACCCTTACCTGGCCCAGCGTGCAGAAGATGTCCGAAGCCTGGGCA
AACGCCTCATTCAGGCCGTATTCGGGCACCCCGGGCAAGAGCCCGGCAAGCTCAAGGTGCCCACCATACTGCTGGCGGAC
GATATCAGCCCGGCTGAGTTTGCCGTACTGCCGCTGGAAAACGTCGCCGGTATCGTGCTCAAGTCCGGCGGTTTAACCAG
CCACACGGCCATTCTGGCAAGGGCCGCGGGCATTCCGGCACTGCTAAGCTGCCCATACAGCGAGCTGGGAATAAACAACG
GTGACCAGCTGGCTATCGACGGTGATGCCGGCGCGCTCTATCGTAACCCTGAAGGTGGAACACTCGAACTGCTGCGCCAG
CATGAAGCATCTGCCCGATTAGCGCGGGAGCAGCTCGACCAGTATCGCGACAAACCCGCCATGACCCAGGATGGCCATGA
GATAAGTCTGCTCGCCAACGTCGGCAACCTGAATGATGTACTCAAGGTGTCAGGCCTTGGCGCCGACGGCATAGGGCTGC
TTCGCACCGAGTTTATGTTGATGCACTCGGCCACCCTCCCCGATGAGCGTGCCCAGTACCAGCTCTACTCTGATGCCATT
CATGCGCTGGAAGGACGGGTATTGACCATACGTACCCTGGATATTGGTGCAGACAAAGAGTTGCCCTGCCTGTGTCAGGT
TAAAGAAGAAAATCCTGCGCTGGGTCTCAGGGGGATTCGCTATACACTCGCCAATCCGCAACTCCTCAAAACCCAGTTAA
GGGCCGTGCTGAGAGCCGCCAATCACGGCCATGTGCGATTGATGTTTCCCATGGTTAATCAGGTGGAAGAGCTGGACGCC
GTGCTGGCTCTGCTGGAAACCTGTCGCCGTGAACTGGACGATGAAGAAAAAGGGTTTGGCGATATCAGCCTCGGCATAGT
GGTGGAAACCCCGGCAGCCGTGCTCAATCTGCCAGCCATGTTACCCATGCTGGACTTTGTCAGCATAGGCACCAACGACC
TCACCCAATATGCCATGGCTGCCGACAGGGGCAACCCTTGCCTTGCCAGGGACTATCCGGCGCTCTCGCCCGCCGTGCTC
AGACTAATCAGCATGACGCTGCAAAGTGCCCGCAGTCAGGATGTGAAGGTATCGCTGTGCGGTGAAATGGGCAGCGATCC
GCGCCTCGTACCGCTGTTAATTGGCCTTGGCTTTGATGAGCTCAGCGTCAATGTGGGAGCCATGCTCGAAGTCAAAGCCG
CGATTTGCCGTCAGGAGTTTGACCGCTGCACCCAGCTTGCCGGACGCGCCCTGATGGCTGACAGATTATCCGAGCTCGAT
GCCTGTATAAGTAGCTATAAATAA

Upstream 100 bases:

>100_bases
GGGTAAACGTCCGGGTATTTGCCCAGGGCCCCCAAGCCGAAGACGCCGTTGAAAGAGTCTCGGCGCTCCTGGTTACTTTA
AGCTGATAGCCGGAGGCATG

Downstream 100 bases:

>100_bases
GCTAAATGTTTCAGTATGATGTATTTTTCGCTGACGCCAAGATCAGCCTATAAACCAAGGGGTACGCAGTCATGGGATTT
TTCAGCCGAATTCGGCGACT

Product: phosphotransferase system, enzyme I

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MQVNGIAIKPGVTFGEALHFNTGHKPLDYRILPIKRIPQQLSRLDGGLARLKQQLSASLQALDSQSDAYALVEADLLYLD
DPDLRAHIADTITQLQFSACVSIERVFAHQASELEALDDPYLAQRAEDVRSLGKRLIQAVFGHPGQEPGKLKVPTILLAD
DISPAEFAVLPLENVAGIVLKSGGLTSHTAILARAAGIPALLSCPYSELGINNGDQLAIDGDAGALYRNPEGGTLELLRQ
HEASARLAREQLDQYRDKPAMTQDGHEISLLANVGNLNDVLKVSGLGADGIGLLRTEFMLMHSATLPDERAQYQLYSDAI
HALEGRVLTIRTLDIGADKELPCLCQVKEENPALGLRGIRYTLANPQLLKTQLRAVLRAANHGHVRLMFPMVNQVEELDA
VLALLETCRRELDDEEKGFGDISLGIVVETPAAVLNLPAMLPMLDFVSIGTNDLTQYAMAADRGNPCLARDYPALSPAVL
RLISMTLQSARSQDVKVSLCGEMGSDPRLVPLLIGLGFDELSVNVGAMLEVKAAICRQEFDRCTQLAGRALMADRLSELD
ACISSYK

Sequences:

>Translated_567_residues
MQVNGIAIKPGVTFGEALHFNTGHKPLDYRILPIKRIPQQLSRLDGGLARLKQQLSASLQALDSQSDAYALVEADLLYLD
DPDLRAHIADTITQLQFSACVSIERVFAHQASELEALDDPYLAQRAEDVRSLGKRLIQAVFGHPGQEPGKLKVPTILLAD
DISPAEFAVLPLENVAGIVLKSGGLTSHTAILARAAGIPALLSCPYSELGINNGDQLAIDGDAGALYRNPEGGTLELLRQ
HEASARLAREQLDQYRDKPAMTQDGHEISLLANVGNLNDVLKVSGLGADGIGLLRTEFMLMHSATLPDERAQYQLYSDAI
HALEGRVLTIRTLDIGADKELPCLCQVKEENPALGLRGIRYTLANPQLLKTQLRAVLRAANHGHVRLMFPMVNQVEELDA
VLALLETCRRELDDEEKGFGDISLGIVVETPAAVLNLPAMLPMLDFVSIGTNDLTQYAMAADRGNPCLARDYPALSPAVL
RLISMTLQSARSQDVKVSLCGEMGSDPRLVPLLIGLGFDELSVNVGAMLEVKAAICRQEFDRCTQLAGRALMADRLSELD
ACISSYK
>Mature_567_residues
MQVNGIAIKPGVTFGEALHFNTGHKPLDYRILPIKRIPQQLSRLDGGLARLKQQLSASLQALDSQSDAYALVEADLLYLD
DPDLRAHIADTITQLQFSACVSIERVFAHQASELEALDDPYLAQRAEDVRSLGKRLIQAVFGHPGQEPGKLKVPTILLAD
DISPAEFAVLPLENVAGIVLKSGGLTSHTAILARAAGIPALLSCPYSELGINNGDQLAIDGDAGALYRNPEGGTLELLRQ
HEASARLAREQLDQYRDKPAMTQDGHEISLLANVGNLNDVLKVSGLGADGIGLLRTEFMLMHSATLPDERAQYQLYSDAI
HALEGRVLTIRTLDIGADKELPCLCQVKEENPALGLRGIRYTLANPQLLKTQLRAVLRAANHGHVRLMFPMVNQVEELDA
VLALLETCRRELDDEEKGFGDISLGIVVETPAAVLNLPAMLPMLDFVSIGTNDLTQYAMAADRGNPCLARDYPALSPAVL
RLISMTLQSARSQDVKVSLCGEMGSDPRLVPLLIGLGFDELSVNVGAMLEVKAAICRQEFDRCTQLAGRALMADRLSELD
ACISSYK

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=571, Percent_Identity=35.3765323992995, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI48994992, Length=538, Percent_Identity=34.2007434944238, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1789193, Length=585, Percent_Identity=32.3076923076923, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI1788726, Length=542, Percent_Identity=32.6568265682657, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI1787994, Length=415, Percent_Identity=25.5421686746988, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 61466; Mature: 61466

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVNGIAIKPGVTFGEALHFNTGHKPLDYRILPIKRIPQQLSRLDGGLARLKQQLSASLQ
CCCCCEEECCCCCCCCEEECCCCCCCCCEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHH
ALDSQSDAYALVEADLLYLDDPDLRAHIADTITQLQFSACVSIERVFAHQASELEALDDP
HHCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
YLAQRAEDVRSLGKRLIQAVFGHPGQEPGKLKVPTILLADDISPAEFAVLPLENVAGIVL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCEEEEECCCCCCCEEEEEHHHHCEEEE
KSGGLTSHTAILARAAGIPALLSCPYSELGINNGDQLAIDGDAGALYRNPEGGTLELLRQ
ECCCCCHHHHHHHHHCCCCHHHHCCHHHHCCCCCCEEEEECCCCCEEECCCCCHHHHHHH
HEASARLAREQLDQYRDKPAMTQDGHEISLLANVGNLNDVLKVSGLGADGIGLLRTEFML
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHEEECCCCCCHHHHHHHHHH
MHSATLPDERAQYQLYSDAIHALEGRVLTIRTLDIGADKELPCLCQVKEENPALGLRGIR
HHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCCEE
YTLANPQLLKTQLRAVLRAANHGHVRLMFPMVNQVEELDAVLALLETCRRELDDEEKGFG
EECCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC
DISLGIVVETPAAVLNLPAMLPMLDFVSIGTNDLTQYAMAADRGNPCLARDYPALSPAVL
CEEEEEEEECCHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
RLISMTLQSARSQDVKVSLCGEMGSDPRLVPLLIGLGFDELSVNVGAMLEVKAAICRQEF
HHHHHHHHHHCCCCCEEEEECCCCCCCCEEEHEEECCCHHHCCCCCHHHHHHHHHHHHHH
DRCTQLAGRALMADRLSELDACISSYK
HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQVNGIAIKPGVTFGEALHFNTGHKPLDYRILPIKRIPQQLSRLDGGLARLKQQLSASLQ
CCCCCEEECCCCCCCCEEECCCCCCCCCEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHH
ALDSQSDAYALVEADLLYLDDPDLRAHIADTITQLQFSACVSIERVFAHQASELEALDDP
HHCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
YLAQRAEDVRSLGKRLIQAVFGHPGQEPGKLKVPTILLADDISPAEFAVLPLENVAGIVL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCEEEEECCCCCCCEEEEEHHHHCEEEE
KSGGLTSHTAILARAAGIPALLSCPYSELGINNGDQLAIDGDAGALYRNPEGGTLELLRQ
ECCCCCHHHHHHHHHCCCCHHHHCCHHHHCCCCCCEEEEECCCCCEEECCCCCHHHHHHH
HEASARLAREQLDQYRDKPAMTQDGHEISLLANVGNLNDVLKVSGLGADGIGLLRTEFML
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHEEECCCCCCHHHHHHHHHH
MHSATLPDERAQYQLYSDAIHALEGRVLTIRTLDIGADKELPCLCQVKEENPALGLRGIR
HHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCCEE
YTLANPQLLKTQLRAVLRAANHGHVRLMFPMVNQVEELDAVLALLETCRRELDDEEKGFG
EECCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC
DISLGIVVETPAAVLNLPAMLPMLDFVSIGTNDLTQYAMAADRGNPCLARDYPALSPAVL
CEEEEEEEECCHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
RLISMTLQSARSQDVKVSLCGEMGSDPRLVPLLIGLGFDELSVNVGAMLEVKAAICRQEF
HHHHHHHHHHCCCCCEEEEECCCCCCCCEEEHEEECCCHHHCCCCCHHHHHHHHHHHHHH
DRCTQLAGRALMADRLSELDACISSYK
HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2457575; 2960675; 9205837; 9278503; 2411636; 3290198; 9298646; 7876255; 8805571; 9054557; 9541412; 10048929 [H]