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The map label for this gene is iaaA [H]

Identifier: 119774754

GI number: 119774754

Start: 1979427

End: 1980410

Strand: Direct

Name: iaaA [H]

Synonym: Sama_1617

Alternate gene names: 119774754

Gene position: 1979427-1980410 (Clockwise)

Preceding gene: 119774751

Following gene: 119774755

Centisome position: 45.97

GC content: 55.18

Gene sequence:

>984_bases
ATGACCCCGGCGTCAGCACAAGAAAATCCCTTTGGCATCGCCATTCATGGCGGGGCCGGTACCATTTCCAAGGCCAGGCT
GACGGACGAGCAGGAGCAGGCCTACCGCGACAAATTGGAAGAAGCGGTAAACGCCGGTCACAAAATTCTGGCCAAGGGCG
GCGACAGTCTGGACGCGGTCAAGGCTGCTATCAATATTCTTGAAGACAGCCCGCTGTTTAATGCCGGTATGGGCGCCGTG
TACACCTTTGACGGTACCCATGAACTCGATGCGTCCATCATGGATGGCAACACCATGAATGCCGGTGCCGTGGCCGGAGT
TAAGCACATCAAAAACCCCATCGATTTAGCGTTGGTGGTGATGAATAAATCTGATCACGTGATGCTCTCCGGTGTCGGCG
CAGAAGAATTTGCCCTGACCCAGGGAATGCCTCTTGTGCCAGCCAACACCTTCGATACCGACAGCCGTTACCAGCAGCTC
CTGGATGCCAAAGCAAAAATTAACGCCGCCGAAAGCGCTGCCAAAGACTTCCATGCATCGGCCACCAGCCTCGACCTTGA
CTACAAGTTCGGCACCGTGGGCGCGGTTGCACTGGACAAAAACGGCAACCTGGCAGCCGGGACCTCGACCGGGGGGATGA
CGGCCAAGCGTTTTGGTCGTATTGGTGACTCCCCGGTTATCGGTGCAGGAACTTATGCCGAGAATGGTGTGTGCGCGGTA
TCTGCTACCGGCCATGGCGAATACTTCATTCGCTATCATGTGGCGGCCGACATTTGTGCACGGATGAAGTATCAGCAAAA
AAATGTCATTCAGGCATCAGATGAGGTGATTAACCAGCGTCTGGTGGAGGCTGGCGGCAGTGGAGGCATTATCGCGATTG
ATGCCCAGGGCAATGTGGCGACGCCCTTTAATACTGAGGGCATGTACCGTGCAAGCCGGGTAAATAAGGACGCTCCCTTG
ATCATGATTTGGCGCGATAAATAA

Upstream 100 bases:

>100_bases
GCGAAATCGTTAAAAAGAACACACAGTTGTCACACTAAAGCAGAATAAGATGAACAAGTATCCCGCGATATTCACAGCCC
TTTTTAGTCTTGGAGTAGTC

Downstream 100 bases:

>100_bases
CAGATTGATGCAATCGAATCCCTCGGCAGGCAATCACCGCAGTTTTTTCCTACACTTGGAAGGTAACAACTGACAGGGGA
ATGCAATGGATTCAATTAAA

Product: asparaginase family protein

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTPASAQENPFGIAIHGGAGTISKARLTDEQEQAYRDKLEEAVNAGHKILAKGGDSLDAVKAAINILEDSPLFNAGMGAV
YTFDGTHELDASIMDGNTMNAGAVAGVKHIKNPIDLALVVMNKSDHVMLSGVGAEEFALTQGMPLVPANTFDTDSRYQQL
LDAKAKINAAESAAKDFHASATSLDLDYKFGTVGAVALDKNGNLAAGTSTGGMTAKRFGRIGDSPVIGAGTYAENGVCAV
SATGHGEYFIRYHVAADICARMKYQQKNVIQASDEVINQRLVEAGGSGGIIAIDAQGNVATPFNTEGMYRASRVNKDAPL
IMIWRDK

Sequences:

>Translated_327_residues
MTPASAQENPFGIAIHGGAGTISKARLTDEQEQAYRDKLEEAVNAGHKILAKGGDSLDAVKAAINILEDSPLFNAGMGAV
YTFDGTHELDASIMDGNTMNAGAVAGVKHIKNPIDLALVVMNKSDHVMLSGVGAEEFALTQGMPLVPANTFDTDSRYQQL
LDAKAKINAAESAAKDFHASATSLDLDYKFGTVGAVALDKNGNLAAGTSTGGMTAKRFGRIGDSPVIGAGTYAENGVCAV
SATGHGEYFIRYHVAADICARMKYQQKNVIQASDEVINQRLVEAGGSGGIIAIDAQGNVATPFNTEGMYRASRVNKDAPL
IMIWRDK
>Mature_326_residues
TPASAQENPFGIAIHGGAGTISKARLTDEQEQAYRDKLEEAVNAGHKILAKGGDSLDAVKAAINILEDSPLFNAGMGAVY
TFDGTHELDASIMDGNTMNAGAVAGVKHIKNPIDLALVVMNKSDHVMLSGVGAEEFALTQGMPLVPANTFDTDSRYQQLL
DAKAKINAAESAAKDFHASATSLDLDYKFGTVGAVALDKNGNLAAGTSTGGMTAKRFGRIGDSPVIGAGTYAENGVCAVS
ATGHGEYFIRYHVAADICARMKYQQKNVIQASDEVINQRLVEAGGSGGIIAIDAQGNVATPFNTEGMYRASRVNKDAPLI
MIWRDK

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Degrades L- isoaspartyl-containing di- and tripeptides. Acts best on iso-Asp- Leu, followed by iso-Asp-Ala, -His and to a lesser extent iso-Asp- Lys, -Phe and

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=304, Percent_Identity=39.1447368421053, Blast_Score=193, Evalue=1e-49,
Organism=Homo sapiens, GI145275200, Length=304, Percent_Identity=39.1447368421053, Blast_Score=193, Evalue=1e-49,
Organism=Homo sapiens, GI98991769, Length=342, Percent_Identity=30.7017543859649, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI285002251, Length=231, Percent_Identity=32.034632034632, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1787050, Length=315, Percent_Identity=53.3333333333333, Blast_Score=317, Evalue=7e-88,
Organism=Caenorhabditis elegans, GI32564649, Length=257, Percent_Identity=26.4591439688716, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI18921183, Length=308, Percent_Identity=34.4155844155844, Blast_Score=167, Evalue=9e-42,
Organism=Drosophila melanogaster, GI21357893, Length=269, Percent_Identity=32.3420074349442, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24652135, Length=235, Percent_Identity=32.3404255319149, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI221330102, Length=235, Percent_Identity=32.3404255319149, Blast_Score=98, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 34339; Mature: 34208

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPASAQENPFGIAIHGGAGTISKARLTDEQEQAYRDKLEEAVNAGHKILAKGGDSLDAV
CCCCCCCCCCEEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
KAAINILEDSPLFNAGMGAVYTFDGTHELDASIMDGNTMNAGAVAGVKHIKNPIDLALVV
HHHHHHHCCCCCCCCCCCEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHCCCCEEEEEE
MNKSDHVMLSGVGAEEFALTQGMPLVPANTFDTDSRYQQLLDAKAKINAAESAAKDFHAS
ECCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCC
ATSLDLDYKFGTVGAVALDKNGNLAAGTSTGGMTAKRFGRIGDSPVIGAGTYAENGVCAV
CEEEEEEEECCCEEEEEECCCCCEEECCCCCCCCHHHHCCCCCCCEEECCCCCCCCEEEE
SATGHGEYFIRYHVAADICARMKYQQKNVIQASDEVINQRLVEAGGSGGIIAIDAQGNVA
EECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
TPFNTEGMYRASRVNKDAPLIMIWRDK
CCCCCCCCEEHHHCCCCCCEEEEEECC
>Mature Secondary Structure 
TPASAQENPFGIAIHGGAGTISKARLTDEQEQAYRDKLEEAVNAGHKILAKGGDSLDAV
CCCCCCCCCEEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
KAAINILEDSPLFNAGMGAVYTFDGTHELDASIMDGNTMNAGAVAGVKHIKNPIDLALVV
HHHHHHHCCCCCCCCCCCEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHCCCCEEEEEE
MNKSDHVMLSGVGAEEFALTQGMPLVPANTFDTDSRYQQLLDAKAKINAAESAAKDFHAS
ECCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCC
ATSLDLDYKFGTVGAVALDKNGNLAAGTSTGGMTAKRFGRIGDSPVIGAGTYAENGVCAV
CEEEEEEEECCCEEEEEECCCCCEEECCCCCCCCHHHHCCCCCCCEEECCCCCCCCEEEE
SATGHGEYFIRYHVAADICARMKYQQKNVIQASDEVINQRLVEAGGSGGIIAIDAQGNVA
EECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
TPFNTEGMYRASRVNKDAPLIMIWRDK
CCCCCCCCEEHHHCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]