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The map label for this gene is cpdB [H]

Identifier: 119774700

GI number: 119774700

Start: 1903084

End: 1905192

Strand: Reverse

Name: cpdB [H]

Synonym: Sama_1563

Alternate gene names: 119774700

Gene position: 1905192-1903084 (Counterclockwise)

Preceding gene: 119774701

Following gene: 119774699

Centisome position: 44.24

GC content: 54.95

Gene sequence:

>2109_bases
ATGGAGCGTGCCCACAGAGGCGCGCCCGGGGTTAACATTTCAAGAGATACGGATATGAATCAAAAACATCTGTTGGCGGT
AACCATTGCCGCTGCATTGGGCGTCAGCGCCTGCGGCTCTGATAATAACGACGTGGAAAAAGAGGTGCTGGTAGACCTGC
GGGTGATGGAGACCACGGATCTGCACACCAACATCATGGACTTCAACTACTACAGCGGCAAAGAAGATCCAACCATAGGC
CTGGCCCGCACCGCCAGCCTTATCAAGGCTGCCGCCCATGAAGCCCCCAACCACGTACTGGTAGACAACGGCGACCTGCT
GCAGGGCAGCCCCATGGGCGACTATATGGCTAAAATCGGCATTGCCGAAGGCGCCGTGCACCCGGCTTACAAGGCTATGA
ATCTGCTGGGATACAGCGTGGGTAACATCGGCAACCATGAATTTAACTATGGCCTGGATTTCCTCGAAAAATCCCTGGCC
GGTGCCGAGTTCCCGTACATCAACGCCAACGTCTACTGCGCCGACGATAACGGCTGCTGGAAAGGCATCAAAAAGGGCGA
GCACCTGTTCACCCCTTACCTGATTGAAGAAAAAGAAGTTATCGACCAAAACGGCGATAAACACGTCATTAAAATCGGCT
ATATCGGCTTTGTACCACCGCAAATCATGCTGTGGGACAAACAAAACCTCACCGGCAAGGTTGAGCCTGCTGACATCCTT
GAAACAGCGCAAAAGTACGTGCCTGAAATGAAAGCCAAAGGGGCCGATATTATTATCGCTATTCCTCACTCAGGTATTGG
CTCGGTGGAAAACCCGGGCGATCCCATGGCTGAAAACGCCACCTATGCCCTCACCCGCGTAGAGGGCATCAATGCCATCA
TGTTTGGCCACAGCCACTCCATCTTCCCGGATGCACGTTACAGTGACCTGCCCAATACCGACGTTGAAAAAGGCCTGCTG
AACGGCGTGGCCGCCGTAATGCCCGGCCGCTGGGGCGATAACCTTGGTGTGGTCGACTTCAAACTGGCCCGCAAAGACGG
CAAGTGGGCAGTGCTCGATGTAAAGACTGAAGCACGCCCCATCTATGACGGTGCCAACAAGGCGCCACTGGTGGATGCCG
ACAAAGACATCCACGATGCCGTGGAGCTTGAGCATCAGGGCACCCTCGCCTTTGTGGATGAACCCATTGGTGTGGCCGCC
GCCGACATGTACAGCTTCCTCACCCTGGTGCAGGACGACCCAACGGTGCAAATCGTGTCTGATGCGCAAATTGCCAACGT
GAAGGCCAAGCTGCCCTCATCTCTCGCGCACTTACCGGTTATTTCGGCCTCGGCCCCCTTCAAGGCCGGTGGCCGCCACG
GTACTACCTCAGATGCCGACCAGTACGTGCAGGTGGACAAGGGCGCGTTAACCTTTAAAAATGCCGCCGACCTTTACCTC
TACCCCAACACCATGGTGGCGGTAAAAGCGACAGGCGCCGAGCTGAAAGACTGGCTCGAGTGCTCAGCCAATCAGTTCAA
CCAAATTGACCCAAACAAGAGCGAGCCGCAGTTCCTCATCAACTGGGCAGGTCACCCTACCTACAACTTCGATGTGCTGG
ACGGCATGACCTACAAGATTGACGTCACCCAACCGAGCAAGTTCGACCGTGACTGCAAAGTGGTGAATGAAAACGCCAAT
CGTATTGTGGATCTGGCCTTTAAAGACAGCGATGGCAAGGTATACAGTGGCGAAGAGCTGCTGACCAAAGAGTTCATTGT
TGCCTCCAACAACTACCGCGCCTTCGGTGGCAAGTTTGCCGGTACCGGCAGTGACCACGTGGTGCTGGAGCTGCCAGATA
CCAACCGTGAAGCACTGGCTGCCTACATCACCGCCGAGTCAAAATACAATGAAACCACCGGCAAGTACGACGGCATGGTT
AACCCTACCGCCGACTACAACTGGGATTTTGTGACCATCAATAGCGCTGTGACGCTGGATGTGCGTTTCGAAACCCAGGA
CAGCGGCAAGGCCGATGCCTTTATCGAGCAAAACAAACAGCGCCCCATGACCAAGGTCGGCAAAGACGAGCTGGGTTATG
CTGTGTATCGTATCGACCTGACCAAGTAA

Upstream 100 bases:

>100_bases
GACACAATTTGATACGCCCTTTCTACGCTTCGGTCACATTTTTGCAAGCTCTAGAGCTGATACTCTTCCCGACCCCAACG
CCGTTGCGCTGGGTTGGATG

Downstream 100 bases:

>100_bases
TCACTGTCTGCAAATGCCAGTGACCCGTTAAAAAGGCGCCCCCCGGCGCCTTTTTTTACCCTCTCAGCGGGTGAGCAGCG
GATAAACATGCCTTTCCCAG

Product: bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MERAHRGAPGVNISRDTDMNQKHLLAVTIAAALGVSACGSDNNDVEKEVLVDLRVMETTDLHTNIMDFNYYSGKEDPTIG
LARTASLIKAAAHEAPNHVLVDNGDLLQGSPMGDYMAKIGIAEGAVHPAYKAMNLLGYSVGNIGNHEFNYGLDFLEKSLA
GAEFPYINANVYCADDNGCWKGIKKGEHLFTPYLIEEKEVIDQNGDKHVIKIGYIGFVPPQIMLWDKQNLTGKVEPADIL
ETAQKYVPEMKAKGADIIIAIPHSGIGSVENPGDPMAENATYALTRVEGINAIMFGHSHSIFPDARYSDLPNTDVEKGLL
NGVAAVMPGRWGDNLGVVDFKLARKDGKWAVLDVKTEARPIYDGANKAPLVDADKDIHDAVELEHQGTLAFVDEPIGVAA
ADMYSFLTLVQDDPTVQIVSDAQIANVKAKLPSSLAHLPVISASAPFKAGGRHGTTSDADQYVQVDKGALTFKNAADLYL
YPNTMVAVKATGAELKDWLECSANQFNQIDPNKSEPQFLINWAGHPTYNFDVLDGMTYKIDVTQPSKFDRDCKVVNENAN
RIVDLAFKDSDGKVYSGEELLTKEFIVASNNYRAFGGKFAGTGSDHVVLELPDTNREALAAYITAESKYNETTGKYDGMV
NPTADYNWDFVTINSAVTLDVRFETQDSGKADAFIEQNKQRPMTKVGKDELGYAVYRIDLTK

Sequences:

>Translated_702_residues
MERAHRGAPGVNISRDTDMNQKHLLAVTIAAALGVSACGSDNNDVEKEVLVDLRVMETTDLHTNIMDFNYYSGKEDPTIG
LARTASLIKAAAHEAPNHVLVDNGDLLQGSPMGDYMAKIGIAEGAVHPAYKAMNLLGYSVGNIGNHEFNYGLDFLEKSLA
GAEFPYINANVYCADDNGCWKGIKKGEHLFTPYLIEEKEVIDQNGDKHVIKIGYIGFVPPQIMLWDKQNLTGKVEPADIL
ETAQKYVPEMKAKGADIIIAIPHSGIGSVENPGDPMAENATYALTRVEGINAIMFGHSHSIFPDARYSDLPNTDVEKGLL
NGVAAVMPGRWGDNLGVVDFKLARKDGKWAVLDVKTEARPIYDGANKAPLVDADKDIHDAVELEHQGTLAFVDEPIGVAA
ADMYSFLTLVQDDPTVQIVSDAQIANVKAKLPSSLAHLPVISASAPFKAGGRHGTTSDADQYVQVDKGALTFKNAADLYL
YPNTMVAVKATGAELKDWLECSANQFNQIDPNKSEPQFLINWAGHPTYNFDVLDGMTYKIDVTQPSKFDRDCKVVNENAN
RIVDLAFKDSDGKVYSGEELLTKEFIVASNNYRAFGGKFAGTGSDHVVLELPDTNREALAAYITAESKYNETTGKYDGMV
NPTADYNWDFVTINSAVTLDVRFETQDSGKADAFIEQNKQRPMTKVGKDELGYAVYRIDLTK
>Mature_702_residues
MERAHRGAPGVNISRDTDMNQKHLLAVTIAAALGVSACGSDNNDVEKEVLVDLRVMETTDLHTNIMDFNYYSGKEDPTIG
LARTASLIKAAAHEAPNHVLVDNGDLLQGSPMGDYMAKIGIAEGAVHPAYKAMNLLGYSVGNIGNHEFNYGLDFLEKSLA
GAEFPYINANVYCADDNGCWKGIKKGEHLFTPYLIEEKEVIDQNGDKHVIKIGYIGFVPPQIMLWDKQNLTGKVEPADIL
ETAQKYVPEMKAKGADIIIAIPHSGIGSVENPGDPMAENATYALTRVEGINAIMFGHSHSIFPDARYSDLPNTDVEKGLL
NGVAAVMPGRWGDNLGVVDFKLARKDGKWAVLDVKTEARPIYDGANKAPLVDADKDIHDAVELEHQGTLAFVDEPIGVAA
ADMYSFLTLVQDDPTVQIVSDAQIANVKAKLPSSLAHLPVISASAPFKAGGRHGTTSDADQYVQVDKGALTFKNAADLYL
YPNTMVAVKATGAELKDWLECSANQFNQIDPNKSEPQFLINWAGHPTYNFDVLDGMTYKIDVTQPSKFDRDCKVVNENAN
RIVDLAFKDSDGKVYSGEELLTKEFIVASNNYRAFGGKFAGTGSDHVVLELPDTNREALAAYITAESKYNETTGKYDGMV
NPTADYNWDFVTINSAVTLDVRFETQDSGKADAFIEQNKQRPMTKVGKDELGYAVYRIDLTK

Specific function: This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'- cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=681, Percent_Identity=53.0102790014684, Blast_Score=725, Evalue=0.0,
Organism=Escherichia coli, GI1786687, Length=491, Percent_Identity=22.8105906313646, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR006294
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16 [H]

Molecular weight: Translated: 76621; Mature: 76621

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERAHRGAPGVNISRDTDMNQKHLLAVTIAAALGVSACGSDNNDVEKEVLVDLRVMETTD
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHCHHHCCCCCCCCCHHHEEEEEEEEECC
LHTNIMDFNYYSGKEDPTIGLARTASLIKAAAHEAPNHVLVDNGDLLQGSPMGDYMAKIG
CCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEECCCCEECCCCCHHHHHHHC
IAEGAVHPAYKAMNLLGYSVGNIGNHEFNYGLDFLEKSLAGAEFPYINANVYCADDNGCW
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCEECCEEEEECCCCHH
KGIKKGEHLFTPYLIEEKEVIDQNGDKHVIKIGYIGFVPPQIMLWDKQNLTGKVEPADIL
HHCCCCCEEECCEEECCHHHHCCCCCEEEEEEEEEECCCCEEEEEECCCCCCCCCHHHHH
ETAQKYVPEMKAKGADIIIAIPHSGIGSVENPGDPMAENATYALTRVEGINAIMFGHSHS
HHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCC
IFPDARYSDLPNTDVEKGLLNGVAAVMPGRWGDNLGVVDFKLARKDGKWAVLDVKTEARP
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCC
IYDGANKAPLVDADKDIHDAVELEHQGTLAFVDEPIGVAAADMYSFLTLVQDDPTVQIVS
CCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEE
DAQIANVKAKLPSSLAHLPVISASAPFKAGGRHGTTSDADQYVQVDKGALTFKNAADLYL
CCEEEEEHHHCCCCHHHCEEEECCCCCCCCCCCCCCCCCCCEEEECCCEEEECCCCEEEE
YPNTMVAVKATGAELKDWLECSANQFNQIDPNKSEPQFLINWAGHPTYNFDVLDGMTYKI
ECCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCEEEEE
DVTQPSKFDRDCKVVNENANRIVDLAFKDSDGKVYSGEELLTKEFIVASNNYRAFGGKFA
EECCCCCCCCCCEEECCCCCEEEEEEEECCCCCEECCHHHHEEEEEEECCCCEECCCEEC
GTGSDHVVLELPDTNREALAAYITAESKYNETTGKYDGMVNPTADYNWDFVTINSAVTLD
CCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEE
VRFETQDSGKADAFIEQNKQRPMTKVGKDELGYAVYRIDLTK
EEEECCCCCCCCHHHHCCCCCCHHHCCCHHCCEEEEEEEECC
>Mature Secondary Structure
MERAHRGAPGVNISRDTDMNQKHLLAVTIAAALGVSACGSDNNDVEKEVLVDLRVMETTD
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHCHHHCCCCCCCCCHHHEEEEEEEEECC
LHTNIMDFNYYSGKEDPTIGLARTASLIKAAAHEAPNHVLVDNGDLLQGSPMGDYMAKIG
CCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEECCCCEECCCCCHHHHHHHC
IAEGAVHPAYKAMNLLGYSVGNIGNHEFNYGLDFLEKSLAGAEFPYINANVYCADDNGCW
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCEECCEEEEECCCCHH
KGIKKGEHLFTPYLIEEKEVIDQNGDKHVIKIGYIGFVPPQIMLWDKQNLTGKVEPADIL
HHCCCCCEEECCEEECCHHHHCCCCCEEEEEEEEEECCCCEEEEEECCCCCCCCCHHHHH
ETAQKYVPEMKAKGADIIIAIPHSGIGSVENPGDPMAENATYALTRVEGINAIMFGHSHS
HHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCC
IFPDARYSDLPNTDVEKGLLNGVAAVMPGRWGDNLGVVDFKLARKDGKWAVLDVKTEARP
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCC
IYDGANKAPLVDADKDIHDAVELEHQGTLAFVDEPIGVAAADMYSFLTLVQDDPTVQIVS
CCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEE
DAQIANVKAKLPSSLAHLPVISASAPFKAGGRHGTTSDADQYVQVDKGALTFKNAADLYL
CCEEEEEHHHCCCCHHHCEEEECCCCCCCCCCCCCCCCCCCEEEECCCEEEECCCCEEEE
YPNTMVAVKATGAELKDWLECSANQFNQIDPNKSEPQFLINWAGHPTYNFDVLDGMTYKI
ECCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCEEEEE
DVTQPSKFDRDCKVVNENANRIVDLAFKDSDGKVYSGEELLTKEFIVASNNYRAFGGKFA
EECCCCCCCCCCEEECCCCCEEEEEEEECCCCCEECCHHHHEEEEEEECCCCEECCCEEC
GTGSDHVVLELPDTNREALAAYITAESKYNETTGKYDGMVNPTADYNWDFVTINSAVTLD
CCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEE
VRFETQDSGKADAFIEQNKQRPMTKVGKDELGYAVYRIDLTK
EEEECCCCCCCCHHHHCCCCCCHHHCCCHHCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA