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The map label for this gene is lpsJ [H]

Identifier: 119774493

GI number: 119774493

Start: 1645192

End: 1645848

Strand: Reverse

Name: lpsJ [H]

Synonym: Sama_1356

Alternate gene names: 119774493

Gene position: 1645848-1645192 (Counterclockwise)

Preceding gene: 119774494

Following gene: 119774491

Centisome position: 38.22

GC content: 56.32

Gene sequence:

>657_bases
ATGGCACTTTCAAGAGAACAACTGGCCCAGCGCGTCGCCAAAGAGCTTAAAGACGGCTACTACGTAAACTTAGGCATCGG
CATCCCTACCTTGGTGGCCAACTATATCCCCGACGGCATGCAGGTGATGCTGCAGTCGGAAAATGGCCTGCTGGGCATGG
GCGAATTCCCCACCGAAGAGACTATCGACCCGGATCTGATTAACGCCGGTAAGCAAACCGTAACAGCGGTAAAAGGCGCA
TCGTTTTTCTCCAGCGCCGAAAGCTTCGCCATGATCCGCGGCGGCCATGTGGACTTAACCGTGCTCGGTGCCTTCGAGGT
GGACGTTGAAGGCTCTATCGCCTCCTGGATGATCCCCGGCAAGCTGGTAAAAGGCATGGGCGGTGCCATGGATTTGGTGG
CCGGTGCCGACAATATTATCGTTACCATGATGCACGCCGATAAATACGGTAACTCCAAGCTGCTGCCCAAGTGCGAGCTG
CCGCTCACCGGCTTTGGCTGTATCAAACGCGTGCTTACCGATCTGGCCTTTATCGAAATCAAAGATGGCGCCTTCCACCT
GCTGGAGCGCGCCCCCGGCGTGACCGTGGAAGAAATCGTCGAGAAGACCGCAGGCAAGCTTATCGTGCCCGAGCACGTAC
CTGAGATGCAGTTCTAA

Upstream 100 bases:

>100_bases
CATATCCACACCCCGGGCATTTATGTGGATCGCATCATCAAAGGCAGCTTTGAGAAGCGTATCGAACAGCGCACCGTGAA
AAAGGCTTAAGGAGACCGCC

Downstream 100 bases:

>100_bases
ACGGCCCAGCGCTTTCAGGCCAGTCCATAAAAACACCCGCAGCTGCGGGTGTTTTTATATGTATAGCCTGCTGCTATTTA
CCAATCCAAACTTGCGACAT

Product: 3-oxoacid CoA-transferase

Products: NA

Alternate protein names: OXCT B; Succinyl CoA:3-oxoacid CoA-transferase [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MALSREQLAQRVAKELKDGYYVNLGIGIPTLVANYIPDGMQVMLQSENGLLGMGEFPTEETIDPDLINAGKQTVTAVKGA
SFFSSAESFAMIRGGHVDLTVLGAFEVDVEGSIASWMIPGKLVKGMGGAMDLVAGADNIIVTMMHADKYGNSKLLPKCEL
PLTGFGCIKRVLTDLAFIEIKDGAFHLLERAPGVTVEEIVEKTAGKLIVPEHVPEMQF

Sequences:

>Translated_218_residues
MALSREQLAQRVAKELKDGYYVNLGIGIPTLVANYIPDGMQVMLQSENGLLGMGEFPTEETIDPDLINAGKQTVTAVKGA
SFFSSAESFAMIRGGHVDLTVLGAFEVDVEGSIASWMIPGKLVKGMGGAMDLVAGADNIIVTMMHADKYGNSKLLPKCEL
PLTGFGCIKRVLTDLAFIEIKDGAFHLLERAPGVTVEEIVEKTAGKLIVPEHVPEMQF
>Mature_217_residues
ALSREQLAQRVAKELKDGYYVNLGIGIPTLVANYIPDGMQVMLQSENGLLGMGEFPTEETIDPDLINAGKQTVTAVKGAS
FFSSAESFAMIRGGHVDLTVLGAFEVDVEGSIASWMIPGKLVKGMGGAMDLVAGADNIIVTMMHADKYGNSKLLPKCELP
LTGFGCIKRVLTDLAFIEIKDGAFHLLERAPGVTVEEIVEKTAGKLIVPEHVPEMQF

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=215, Percent_Identity=52.093023255814, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI11545841, Length=216, Percent_Identity=49.537037037037, Blast_Score=214, Evalue=4e-56,
Organism=Escherichia coli, GI1788552, Length=206, Percent_Identity=47.5728155339806, Blast_Score=203, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI17531517, Length=216, Percent_Identity=54.6296296296296, Blast_Score=221, Evalue=3e-58,
Organism=Drosophila melanogaster, GI21358325, Length=214, Percent_Identity=56.5420560747664, Blast_Score=245, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24655794, Length=214, Percent_Identity=56.5420560747664, Blast_Score=245, Evalue=2e-65,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.5 [H]

Molecular weight: Translated: 23399; Mature: 23267

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSREQLAQRVAKELKDGYYVNLGIGIPTLVANYIPDGMQVMLQSENGLLGMGEFPTEE
CCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCC
TIDPDLINAGKQTVTAVKGASFFSSAESFAMIRGGHVDLTVLGAFEVDVEGSIASWMIPG
CCCHHHHHCCCHHHHHHHCCHHHHHHHHHHEEECCCEEEEEEEEEEEEECCCHHHEECCH
KLVKGMGGAMDLVAGADNIIVTMMHADKYGNSKLLPKCELPLTGFGCIKRVLTDLAFIEI
HHHHCCCCHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEEEEE
KDGAFHLLERAPGVTVEEIVEKTAGKLIVPEHVPEMQF
CCCHHHHHHHCCCCCHHHHHHHHCCCEECCCCCCCCCC
>Mature Secondary Structure 
ALSREQLAQRVAKELKDGYYVNLGIGIPTLVANYIPDGMQVMLQSENGLLGMGEFPTEE
CCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCC
TIDPDLINAGKQTVTAVKGASFFSSAESFAMIRGGHVDLTVLGAFEVDVEGSIASWMIPG
CCCHHHHHCCCHHHHHHHCCHHHHHHHHHHEEECCCEEEEEEEEEEEEECCCHHHEECCH
KLVKGMGGAMDLVAGADNIIVTMMHADKYGNSKLLPKCELPLTGFGCIKRVLTDLAFIEI
HHHHCCCCHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEEEEE
KDGAFHLLERAPGVTVEEIVEKTAGKLIVPEHVPEMQF
CCCHHHHHHHCCCCCHHHHHHHHCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9385134 [H]