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The map label for this gene is bar [H]

Identifier: 119774479

GI number: 119774479

Start: 1634681

End: 1635196

Strand: Reverse

Name: bar [H]

Synonym: Sama_1342

Alternate gene names: 119774479

Gene position: 1635196-1634681 (Counterclockwise)

Preceding gene: 119774480

Following gene: 119774478

Centisome position: 37.97

GC content: 51.36

Gene sequence:

>516_bases
ATGATAAGACATGCCGCTCTACACGATGCCAGCGCCGTTGCCGACATCTACAACCATTACATCGAAAGCACCGCCATCAC
CTTTGAAGAAACACCCCTTCAGGCAGCAGAGATAGCAGCTCGAATTCAGCATGTGCAAGCAGCTGGCCTGCCCTGGCTGG
TTGCCTTGGAGGGCAACGCCCTTACCGGCTATGCCTATGCGACCAAATGGAAAGAGCGAAGTGCTTACCGGTTCACAGTT
GAAACCACGGTTTATGTTGCTCCCAACGGACATGGGAAAGGTGTCGGTACGGCTTTGTATCAGGCACTTATCGAGCGGCT
GAAAATCCTGAAAGTAAACAGCGTGATTGGCGGCATTACCCTGCCAAATCCGGCCAGCATTGCCCTACACGAAAAAATGG
GCATGAAAAAAGTCGCACATTTTCAAAATATTGGCTACAAGTTTGGCCAATGGCAAGACGTTGGTTACTGGCAGCTTAAC
TTGCAGACAGTGCCAGGTCTGGACTCATCGCCCTGA

Upstream 100 bases:

>100_bases
CGCCTGCGGGACAAATTTTCTCGGGCTAGAAAAGGCCGCGATTGAGCCCCTCCCCCGCCGCACCTGTCAATCCATAACTG
AAGTTAGTTGGTAAGTTCAG

Downstream 100 bases:

>100_bases
CTGCGCCACGCCGTACTTTCAATGCTGGCTTTTTAAGCCTTTTAAGTGACGCATCCCCTGCGTTATCTCCCCGGGTATGG
TTACAGGCCCCGTTCAATTT

Product: phosphinothricin N-acetyltransferase

Products: NA

Alternate protein names: PPT N-acetyltransferase; Phosphinothricin-resistance protein [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MIRHAALHDASAVADIYNHYIESTAITFEETPLQAAEIAARIQHVQAAGLPWLVALEGNALTGYAYATKWKERSAYRFTV
ETTVYVAPNGHGKGVGTALYQALIERLKILKVNSVIGGITLPNPASIALHEKMGMKKVAHFQNIGYKFGQWQDVGYWQLN
LQTVPGLDSSP

Sequences:

>Translated_171_residues
MIRHAALHDASAVADIYNHYIESTAITFEETPLQAAEIAARIQHVQAAGLPWLVALEGNALTGYAYATKWKERSAYRFTV
ETTVYVAPNGHGKGVGTALYQALIERLKILKVNSVIGGITLPNPASIALHEKMGMKKVAHFQNIGYKFGQWQDVGYWQLN
LQTVPGLDSSP
>Mature_171_residues
MIRHAALHDASAVADIYNHYIESTAITFEETPLQAAEIAARIQHVQAAGLPWLVALEGNALTGYAYATKWKERSAYRFTV
ETTVYVAPNGHGKGVGTALYQALIERLKILKVNSVIGGITLPNPASIALHEKMGMKKVAHFQNIGYKFGQWQDVGYWQLN
LQTVPGLDSSP

Specific function: This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance. Inactivates PTT by transfer of an acetyl group [H]

COG id: COG1247

COG function: function code M; Sortase and related acyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1787719, Length=161, Percent_Identity=36.0248447204969, Blast_Score=106, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181 [H]

Pfam domain/function: PF00583 Acetyltransf_1 [H]

EC number: =2.3.1.183 [H]

Molecular weight: Translated: 18763; Mature: 18763

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRHAALHDASAVADIYNHYIESTAITFEETPLQAAEIAARIQHVQAAGLPWLVALEGNA
CCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCE
LTGYAYATKWKERSAYRFTVETTVYVAPNGHGKGVGTALYQALIERLKILKVNSVIGGIT
EECHHEEECCCCCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEECHHCCCCC
LPNPASIALHEKMGMKKVAHFQNIGYKFGQWQDVGYWQLNLQTVPGLDSSP
CCCCCCEEEHHHHCHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure
MIRHAALHDASAVADIYNHYIESTAITFEETPLQAAEIAARIQHVQAAGLPWLVALEGNA
CCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCE
LTGYAYATKWKERSAYRFTVETTVYVAPNGHGKGVGTALYQALIERLKILKVNSVIGGIT
EECHHEEECCCCCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEECHHCCCCC
LPNPASIALHEKMGMKKVAHFQNIGYKFGQWQDVGYWQLNLQTVPGLDSSP
CCCCCCEEEHHHHCHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2315036 [H]