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The map label for this gene is dusC [H]

Identifier: 119774440

GI number: 119774440

Start: 1587305

End: 1588273

Strand: Reverse

Name: dusC [H]

Synonym: Sama_1303

Alternate gene names: 119774440

Gene position: 1588273-1587305 (Counterclockwise)

Preceding gene: 119774456

Following gene: 119774439

Centisome position: 36.88

GC content: 54.49

Gene sequence:

>969_bases
ATGCGGGATATCAGCTTGCGGATTATTTTGGCCCCTATGGAGGGCGTGCTGGATCATTTGATGAGGGAGCTGCTCTCATC
CATCAACCCCTACGATCTCCTGGTTACCGAGTTTGTGCGCGTGGTGGATCAGCTATTACCGGAAAAGGTCTACTATCGCC
TCTGTCCTGAGCTGAAAAACAACGGCCTGACCAAGAGTGGTACCCCGGTTCGGGTTCAGATATTGGGTCAGGAACCCGGT
TGGATGGCTGAAAACGCGCTGCGGGCGGTGACACTGGGCTCCCGAGGTGTAGATGCCAACTTTGGCTGCCCGGCCAAAAT
GGTTAACCGCAGCAAGGGTGGTGCCGTGCTGCTGCAATATCCCGAATTAATTCACGATATTGTCAAAGCCATGCGTCAGG
CTGTGCCCAGCGAGCAGCCGGTCACCGCCAAAATTCGTTTAGGGTACGAAGACAAATCTCTGTTTATGGAAAACGCCCTG
GCCGTATACGAGGCCGGCGCCACCGAGCTTGCCATTCATGCCCGCAGCAAGGTAGACGGTTACAAACCGCCCGCCTATTG
GGAGTACATTACCGAGGTGCGTGAGCGCTTGCCCATTCCGGTCATCGCCAACGGCGAAATCTGGAACCGTGACGATGCCC
TGCGCTGCATGGAAGTAACGGGCTGCGACAGCATCATGATTGGTCGGGGTGCCTTGTCAATGCCAAACCTGGCTGCCAGC
ATTAAGGGTGACACACCCTATGATTGGGCCACAACGCTGAGATTGATGATCCACTACTCAGAGCTGGAAACCTCAGGACT
GAAGGCCACTTATTATCCGGCACGTATCAAACAGTGGTTCAGTTACCTGAGCAGGGAATATCCGGAAGCGGACGACCTGT
TCAGGGAAATACGAATTCACAAAACCACCGAGGACATACTGAATGGCCTCAAACTGGCAGCCACCAAGCTGGCGCCAATG
AAGTCATAA

Upstream 100 bases:

>100_bases
TCATGGGTATCAAGTTCTTGGAATACGAGTTGTCCCAATCTTTTTCCATGCTTGGTAAAGCTGCGGGCGCGCGGTAAAAT
GCCGCGCTTATTTTTAACGA

Downstream 100 bases:

>100_bases
TGGGCGGTACTTAAACAGCGCCGCACGCTTGCAACAGTGGCAATCTTTGATCTGCAAAGGCTTTACACGCTGCTGAAATG
AAAAATCGAACAGTGTTTTG

Product: NifR3/Smm1 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MRDISLRIILAPMEGVLDHLMRELLSSINPYDLLVTEFVRVVDQLLPEKVYYRLCPELKNNGLTKSGTPVRVQILGQEPG
WMAENALRAVTLGSRGVDANFGCPAKMVNRSKGGAVLLQYPELIHDIVKAMRQAVPSEQPVTAKIRLGYEDKSLFMENAL
AVYEAGATELAIHARSKVDGYKPPAYWEYITEVRERLPIPVIANGEIWNRDDALRCMEVTGCDSIMIGRGALSMPNLAAS
IKGDTPYDWATTLRLMIHYSELETSGLKATYYPARIKQWFSYLSREYPEADDLFREIRIHKTTEDILNGLKLAATKLAPM
KS

Sequences:

>Translated_322_residues
MRDISLRIILAPMEGVLDHLMRELLSSINPYDLLVTEFVRVVDQLLPEKVYYRLCPELKNNGLTKSGTPVRVQILGQEPG
WMAENALRAVTLGSRGVDANFGCPAKMVNRSKGGAVLLQYPELIHDIVKAMRQAVPSEQPVTAKIRLGYEDKSLFMENAL
AVYEAGATELAIHARSKVDGYKPPAYWEYITEVRERLPIPVIANGEIWNRDDALRCMEVTGCDSIMIGRGALSMPNLAAS
IKGDTPYDWATTLRLMIHYSELETSGLKATYYPARIKQWFSYLSREYPEADDLFREIRIHKTTEDILNGLKLAATKLAPM
KS
>Mature_322_residues
MRDISLRIILAPMEGVLDHLMRELLSSINPYDLLVTEFVRVVDQLLPEKVYYRLCPELKNNGLTKSGTPVRVQILGQEPG
WMAENALRAVTLGSRGVDANFGCPAKMVNRSKGGAVLLQYPELIHDIVKAMRQAVPSEQPVTAKIRLGYEDKSLFMENAL
AVYEAGATELAIHARSKVDGYKPPAYWEYITEVRERLPIPVIANGEIWNRDDALRCMEVTGCDSIMIGRGALSMPNLAAS
IKGDTPYDWATTLRLMIHYSELETSGLKATYYPARIKQWFSYLSREYPEADDLFREIRIHKTTEDILNGLKLAATKLAPM
KS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=230, Percent_Identity=32.6086956521739, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI239788462, Length=230, Percent_Identity=32.6086956521739, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI31742496, Length=193, Percent_Identity=30.0518134715026, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI40807366, Length=207, Percent_Identity=31.8840579710145, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI8923374, Length=214, Percent_Identity=28.5046728971963, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1788462, Length=308, Percent_Identity=54.5454545454545, Blast_Score=343, Evalue=7e-96,
Organism=Escherichia coli, GI1789660, Length=309, Percent_Identity=33.0097087378641, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI145693211, Length=336, Percent_Identity=28.2738095238095, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=254, Percent_Identity=31.8897637795276, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17507177, Length=179, Percent_Identity=34.0782122905028, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=164, Percent_Identity=29.2682926829268, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=173, Percent_Identity=32.9479768786127, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323433, Length=185, Percent_Identity=26.4864864864865, Blast_Score=66, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323437, Length=197, Percent_Identity=28.9340101522843, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24580595, Length=185, Percent_Identity=34.0540540540541, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19920448, Length=185, Percent_Identity=34.0540540540541, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24585320, Length=234, Percent_Identity=30.3418803418803, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19921524, Length=148, Percent_Identity=33.1081081081081, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36204; Mature: 36204

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDISLRIILAPMEGVLDHLMRELLSSINPYDLLVTEFVRVVDQLLPEKVYYRLCPELKN
CCCEEEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHC
NGLTKSGTPVRVQILGQEPGWMAENALRAVTLGSRGVDANFGCPAKMVNRSKGGAVLLQY
CCCCCCCCCEEEEEECCCCCCHHHHHHHEEEECCCCCCCCCCCCHHHHCCCCCCEEEEEC
PELIHDIVKAMRQAVPSEQPVTAKIRLGYEDKSLFMENALAVYEAGATELAIHARSKVDG
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHEEEEEHHCCCC
YKPPAYWEYITEVRERLPIPVIANGEIWNRDDALRCMEVTGCDSIMIGRGALSMPNLAAS
CCCCHHHHHHHHHHHHCCCCEEECCCEECCCCCCEEEEECCCCEEEECCCCCCCCCCCEE
IKGDTPYDWATTLRLMIHYSELETSGLKATYYPARIKQWFSYLSREYPEADDLFREIRIH
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHC
KTTEDILNGLKLAATKLAPMKS
CCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRDISLRIILAPMEGVLDHLMRELLSSINPYDLLVTEFVRVVDQLLPEKVYYRLCPELKN
CCCEEEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHC
NGLTKSGTPVRVQILGQEPGWMAENALRAVTLGSRGVDANFGCPAKMVNRSKGGAVLLQY
CCCCCCCCCEEEEEECCCCCCHHHHHHHEEEECCCCCCCCCCCCHHHHCCCCCCEEEEEC
PELIHDIVKAMRQAVPSEQPVTAKIRLGYEDKSLFMENALAVYEAGATELAIHARSKVDG
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHEEEEEHHCCCC
YKPPAYWEYITEVRERLPIPVIANGEIWNRDDALRCMEVTGCDSIMIGRGALSMPNLAAS
CCCCHHHHHHHHHHHHCCCCEEECCCEECCCCCCEEEEECCCCEEEECCCCCCCCCCCEE
IKGDTPYDWATTLRLMIHYSELETSGLKATYYPARIKQWFSYLSREYPEADDLFREIRIH
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHC
KTTEDILNGLKLAATKLAPMKS
CCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]