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The map label for this gene is dosC [H]

Identifier: 119774295

GI number: 119774295

Start: 1411827

End: 1412738

Strand: Reverse

Name: dosC [H]

Synonym: Sama_1158

Alternate gene names: 119774295

Gene position: 1412738-1411827 (Counterclockwise)

Preceding gene: 119774297

Following gene: 119774293

Centisome position: 32.81

GC content: 55.26

Gene sequence:

>912_bases
ATGGACTTTGGTATTGCCGCACAAGTCTATCCCGATGATTACCACTTTGGCCGCAGCGCCACCGTGGTCGACATCCACGA
TGGCCCCGATCAGCTTGCACTGATCAACATAGTTCAGCAGCTCCATGCGAGCCTGGATCCAAGGACCGTTTTTGCCTGTT
TTGGCAAAGTCCTGGGCCAGTATTTGCCGCTTGCCGGCATTCAGGCGCAATTTGCCGAAATGCAGCTCAGCTGGGGAAAG
CGCATTGGGGTGAGTTTGAAACGGCATCTCAACAACGAGATCTGCCGCAGTGCTGTGCTGGATTACCGTCTCGATGCGCC
GCTGACACCGGCGCAAATTCGCGTTTTGCAAACGCTGGAGTCCATGTTGGTCCAACCGCTGCTCAATGCGATTCAGTACC
AAAAGATGTCTGAGCAGGCGATGTTTGATACCCTGACAGGCCTTGGAAACCGTAACTTTTACGGCCAGGCCATCAAGCAT
GCGGTGGCAAGGGCGAGCCGTCATCAGGACAAGTTATCCCTGATAGTCCTGGACCTGGACAACTTCAAACAGCTCAATGA
TACCCACGGCCACCAGTTCGGTGATGCCGTGCTCCGTCAATTCGGCGCGCTTATCAAGTCGGCTATCCGCAATACCGATC
AGGCCTTCCGCATTGGCGGCGATGAATTTGTGGTGTTGGTGCGGGGCGAGCTGGACTCGGCGACCTTGCTGTGCGAGCGC
ATATTAAGCAAACTGGCCAAAGGCAGTTTCTTTTACCGAAACCGGATACACACCAGCCTTGGCGCTGCAGAGATCCAGGC
GGGAGAAAACCAGGATAATCTCTACGAACGGGCCGACAAGGCCTTGTATCAAGCCAAGGCCGCCGGCCGCAATTGCTATC
GCCTCAGTCTTCAACACCCTGTCAAAGGCTGA

Upstream 100 bases:

>100_bases
AACTGAGTGACATACAAAGACAAATTTATTTAACGGCTTTTTCGGCACACATTTTGCTTAATTCAAACCAGATAAGTCCT
GAACCGCCGGAGTAATAAAT

Downstream 100 bases:

>100_bases
CAATAAATTGACAGTGGTCGCCCAGGGATGGGCAAGAGTCTCCTTCCAGTTGATTTAAAAACCCCGTAACTGAATACGCC
GCAGAAATTCGGTCATCACC

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MDFGIAAQVYPDDYHFGRSATVVDIHDGPDQLALINIVQQLHASLDPRTVFACFGKVLGQYLPLAGIQAQFAEMQLSWGK
RIGVSLKRHLNNEICRSAVLDYRLDAPLTPAQIRVLQTLESMLVQPLLNAIQYQKMSEQAMFDTLTGLGNRNFYGQAIKH
AVARASRHQDKLSLIVLDLDNFKQLNDTHGHQFGDAVLRQFGALIKSAIRNTDQAFRIGGDEFVVLVRGELDSATLLCER
ILSKLAKGSFFYRNRIHTSLGAAEIQAGENQDNLYERADKALYQAKAAGRNCYRLSLQHPVKG

Sequences:

>Translated_303_residues
MDFGIAAQVYPDDYHFGRSATVVDIHDGPDQLALINIVQQLHASLDPRTVFACFGKVLGQYLPLAGIQAQFAEMQLSWGK
RIGVSLKRHLNNEICRSAVLDYRLDAPLTPAQIRVLQTLESMLVQPLLNAIQYQKMSEQAMFDTLTGLGNRNFYGQAIKH
AVARASRHQDKLSLIVLDLDNFKQLNDTHGHQFGDAVLRQFGALIKSAIRNTDQAFRIGGDEFVVLVRGELDSATLLCER
ILSKLAKGSFFYRNRIHTSLGAAEIQAGENQDNLYERADKALYQAKAAGRNCYRLSLQHPVKG
>Mature_303_residues
MDFGIAAQVYPDDYHFGRSATVVDIHDGPDQLALINIVQQLHASLDPRTVFACFGKVLGQYLPLAGIQAQFAEMQLSWGK
RIGVSLKRHLNNEICRSAVLDYRLDAPLTPAQIRVLQTLESMLVQPLLNAIQYQKMSEQAMFDTLTGLGNRNFYGQAIKH
AVARASRHQDKLSLIVLDLDNFKQLNDTHGHQFGDAVLRQFGALIKSAIRNTDQAFRIGGDEFVVLVRGELDSATLLCER
ILSKLAKGSFFYRNRIHTSLGAAEIQAGENQDNLYERADKALYQAKAAGRNCYRLSLQHPVKG

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=37.3417721518987, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI87081881, Length=165, Percent_Identity=40.6060606060606, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87082007, Length=160, Percent_Identity=40, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788381, Length=172, Percent_Identity=30.8139534883721, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787262, Length=156, Percent_Identity=33.974358974359, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787541, Length=152, Percent_Identity=36.8421052631579, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=31.055900621118, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87081974, Length=166, Percent_Identity=31.9277108433735, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786584, Length=196, Percent_Identity=29.5918367346939, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787802, Length=213, Percent_Identity=25.8215962441315, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787056, Length=158, Percent_Identity=32.9113924050633, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87081977, Length=156, Percent_Identity=32.6923076923077, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 33811; Mature: 33811

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFGIAAQVYPDDYHFGRSATVVDIHDGPDQLALINIVQQLHASLDPRTVFACFGKVLGQ
CCCCCEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YLPLAGIQAQFAEMQLSWGKRIGVSLKRHLNNEICRSAVLDYRLDAPLTPAQIRVLQTLE
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
SMLVQPLLNAIQYQKMSEQAMFDTLTGLGNRNFYGQAIKHAVARASRHQDKLSLIVLDLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECC
NFKQLNDTHGHQFGDAVLRQFGALIKSAIRNTDQAFRIGGDEFVVLVRGELDSATLLCER
CCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCEEEEEEECCCCHHHHHHHH
ILSKLAKGSFFYRNRIHTSLGAAEIQAGENQDNLYERADKALYQAKAAGRNCYRLSLQHP
HHHHHHCCCHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
VKG
CCC
>Mature Secondary Structure
MDFGIAAQVYPDDYHFGRSATVVDIHDGPDQLALINIVQQLHASLDPRTVFACFGKVLGQ
CCCCCEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YLPLAGIQAQFAEMQLSWGKRIGVSLKRHLNNEICRSAVLDYRLDAPLTPAQIRVLQTLE
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
SMLVQPLLNAIQYQKMSEQAMFDTLTGLGNRNFYGQAIKHAVARASRHQDKLSLIVLDLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECC
NFKQLNDTHGHQFGDAVLRQFGALIKSAIRNTDQAFRIGGDEFVVLVRGELDSATLLCER
CCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCEEEEEEECCCCHHHHHHHH
ILSKLAKGSFFYRNRIHTSLGAAEIQAGENQDNLYERADKALYQAKAAGRNCYRLSLQHP
HHHHHHCCCHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
VKG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]