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The map label for this gene is tpiA

Identifier: 119774094

GI number: 119774094

Start: 1157568

End: 1158350

Strand: Direct

Name: tpiA

Synonym: Sama_0957

Alternate gene names: 119774094

Gene position: 1157568-1158350 (Clockwise)

Preceding gene: 119774093

Following gene: 119774095

Centisome position: 26.88

GC content: 50.57

Gene sequence:

>783_bases
ATGGCACTCAGACGTCCAATGGTAGCAGGCAACTGGAAGATGAATGGCAGCGCAGCGCTGGCACAGGAACTCTTCAAAAA
GTTTGCCGTTAAGCTCCAAAACGATTCAGCGGAAGTTGTCCTGTGTCCGCCGACAATTTACCTCGAGAGCGTCAGGCAGT
TGCTCGAAGCGAATAAAGAAGCCCTAAATGGTTGTCTTGTTCGAATGGGGGCTCAGAATTTGAGTCAACATGACTTCGGT
GCTTACACCGGGGAGATTTCCGGGCAGATGCTGAAAGATTCCGGATGCCGATATGTGATTGTCGGGCATTCAGAGCGGCG
AAGAATGTATGGTGAAACCAGTGATATAGTTGCTGAGAAGTTCGCCGCCGCCCAAAAGCACGGCTTGACTCCAATACTTT
GTGTTGGTGAGTCAGGCCCAGCTCGAGAAGCCAGAAGAACCTTTGAGGTGATAGCTGAAGAGCTGGACGTGGTAATCGAA
AAGAACGGTACCATGGCTTTTGATAACGCCATTATCGCCTATGAGCCCCTGTGGGCAGTAGGGACAGGTAAAAGCGCTAC
ACCAGAGCAAGCCCAGGAAGTTCACGCGTTTATTCGCAGGAGGCTCTCTGAAGTTTCTCCCTTCATTGGGGAGAATGTCA
GGATTCTGTATGGCGGAAGTGTAACGCCAAGCAATGCGGCGGATTTATTCGCCCAACCTGATGTTGACGGTGGACTGATT
GGCGGAGCCAGTTTAAACTCGACCGAGTTTTTAACTCTGTGTTCCATAGCAATGAGCGCATAA

Upstream 100 bases:

>100_bases
AAGCAACAGTCGGGCCTGTTTTTGCCAAAAAGTCGAAAATAGGTGTTGTAACTTAAAACGCGGTTCGCTATTATTCGCTC
CGCTTTCAGAGGAGATGGAG

Downstream 100 bases:

>100_bases
TATGTACGAAGTACTGATAGTTATCTACTTGTTGGTTGCACTTGGCCTGATTGGTCTGGTTCTCATCCAGCAAGGTAAAG
GGGCTGACATGGGTGCGTCA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MALRRPMVAGNWKMNGSAALAQELFKKFAVKLQNDSAEVVLCPPTIYLESVRQLLEANKEALNGCLVRMGAQNLSQHDFG
AYTGEISGQMLKDSGCRYVIVGHSERRRMYGETSDIVAEKFAAAQKHGLTPILCVGESGPAREARRTFEVIAEELDVVIE
KNGTMAFDNAIIAYEPLWAVGTGKSATPEQAQEVHAFIRRRLSEVSPFIGENVRILYGGSVTPSNAADLFAQPDVDGGLI
GGASLNSTEFLTLCSIAMSA

Sequences:

>Translated_260_residues
MALRRPMVAGNWKMNGSAALAQELFKKFAVKLQNDSAEVVLCPPTIYLESVRQLLEANKEALNGCLVRMGAQNLSQHDFG
AYTGEISGQMLKDSGCRYVIVGHSERRRMYGETSDIVAEKFAAAQKHGLTPILCVGESGPAREARRTFEVIAEELDVVIE
KNGTMAFDNAIIAYEPLWAVGTGKSATPEQAQEVHAFIRRRLSEVSPFIGENVRILYGGSVTPSNAADLFAQPDVDGGLI
GGASLNSTEFLTLCSIAMSA
>Mature_259_residues
ALRRPMVAGNWKMNGSAALAQELFKKFAVKLQNDSAEVVLCPPTIYLESVRQLLEANKEALNGCLVRMGAQNLSQHDFGA
YTGEISGQMLKDSGCRYVIVGHSERRRMYGETSDIVAEKFAAAQKHGLTPILCVGESGPAREARRTFEVIAEELDVVIEK
NGTMAFDNAIIAYEPLWAVGTGKSATPEQAQEVHAFIRRRLSEVSPFIGENVRILYGGSVTPSNAADLFAQPDVDGGLIG
GASLNSTEFLTLCSIAMSA

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=252, Percent_Identity=43.6507936507937, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI226529917, Length=252, Percent_Identity=43.6507936507937, Blast_Score=194, Evalue=6e-50,
Organism=Escherichia coli, GI1790353, Length=259, Percent_Identity=46.7181467181467, Blast_Score=249, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI17536593, Length=244, Percent_Identity=45.4918032786885, Blast_Score=205, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6320255, Length=253, Percent_Identity=39.5256916996047, Blast_Score=180, Evalue=2e-46,
Organism=Drosophila melanogaster, GI28572008, Length=251, Percent_Identity=44.6215139442231, Blast_Score=204, Evalue=4e-53,
Organism=Drosophila melanogaster, GI28572006, Length=251, Percent_Identity=44.6215139442231, Blast_Score=204, Evalue=4e-53,
Organism=Drosophila melanogaster, GI28572004, Length=251, Percent_Identity=44.6215139442231, Blast_Score=204, Evalue=6e-53,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_SHEAM (A1S458)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926834.1
- ProteinModelPortal:   A1S458
- SMR:   A1S458
- STRING:   A1S458
- GeneID:   4603209
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0957
- NMPDR:   fig|326297.7.peg.898
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   IEKNGTM
- PhylomeDB:   A1S458
- ProtClustDB:   PRK14566
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 28100; Mature: 27969

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 103-103 ACT_SITE 175-175 BINDING 11-11 BINDING 13-13

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRRPMVAGNWKMNGSAALAQELFKKFAVKLQNDSAEVVLCPPTIYLESVRQLLEANKE
CCCCCCCCCCCEEECCHHHHHHHHHHHHHHEECCCCCCEEEECCHHHHHHHHHHHHHCHH
ALNGCLVRMGAQNLSQHDFGAYTGEISGQMLKDSGCRYVIVGHSERRRMYGETSDIVAEK
HHHHHHHHHCHHHCCCCCCCCCCCCCCCEEECCCCCEEEEECCCHHHHHCCCHHHHHHHH
FAAAQKHGLTPILCVGESGPAREARRTFEVIAEELDVVIEKNGTMAFDNAIIAYEPLWAV
HHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEECCCCEEECCEEEEECCEEEE
GTGKSATPEQAQEVHAFIRRRLSEVSPFIGENVRILYGGSVTPSNAADLFAQPDVDGGLI
CCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCCEEEEECCCCCCCCCHHCCCCCCCCCCEE
GGASLNSTEFLTLCSIAMSA
CCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
ALRRPMVAGNWKMNGSAALAQELFKKFAVKLQNDSAEVVLCPPTIYLESVRQLLEANKE
CCCCCCCCCCEEECCHHHHHHHHHHHHHHEECCCCCCEEEECCHHHHHHHHHHHHHCHH
ALNGCLVRMGAQNLSQHDFGAYTGEISGQMLKDSGCRYVIVGHSERRRMYGETSDIVAEK
HHHHHHHHHCHHHCCCCCCCCCCCCCCCEEECCCCCEEEEECCCHHHHHCCCHHHHHHHH
FAAAQKHGLTPILCVGESGPAREARRTFEVIAEELDVVIEKNGTMAFDNAIIAYEPLWAV
HHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEECCCCEEECCEEEEECCEEEE
GTGKSATPEQAQEVHAFIRRRLSEVSPFIGENVRILYGGSVTPSNAADLFAQPDVDGGLI
CCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCCEEEEECCCCCCCCCHHCCCCCCCCCCEE
GGASLNSTEFLTLCSIAMSA
CCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA