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The map label for this gene is rrmJ

Identifier: 119774090

GI number: 119774090

Start: 1152464

End: 1153093

Strand: Direct

Name: rrmJ

Synonym: Sama_0953

Alternate gene names: 119774090

Gene position: 1152464-1153093 (Clockwise)

Preceding gene: 119774088

Following gene: 119774091

Centisome position: 26.76

GC content: 53.02

Gene sequence:

>630_bases
ATGACAGCTAAAAAACGTTCGGCCAGCTCCACCCGTTGGATGCAGGAGCATTTCGATGACCACTATGTGAAATTGGCACA
AAAACGGGGCTTACGCTCTCGTGCCGCCTTCAAGCTCGAAGAATTACAGGAAAAGGATCATCTGATAAAGCCCGGCATGA
CGGTGGTGGATTTGGGAGCAGCCCCCGGTGGCTGGTCTCAGGTTGCGGTCAAACTCGTGGGTGATAGGGGTAAGGTGATA
GCCTGTGATATCCTGCCTATGGACCCCATTGTTGGCGTTGATTTTCTGCAGGGTGATTTCCGCGAAGATGCCGTGCTTAA
CGCATTGCTCGAGCGGGTGGGTGACGAGAAGGTAGATGTTGTGCTGTCTGATATGGCACCCAACATGAGTGGTTCTGATG
GGGTCGATCAGCCCCGAGCCATGTATCTGGTGGAATTGGCACTGGATATGTGTCATCAGGTATTGGCGCCCAATGGCAGC
TTTGCCGTAAAAGTCTTTCAGGGGGAGGGCTTTGACGAGTACATGAAAGCGGTCAGAGAGGCGTTTAAGGTCGTTAAGAC
ACGTAAACCGGACTCATCACGGGCACGTTCCCGTGAGGTCTATCTGGTGGCGACCGGGTATAAGTTGTAG

Upstream 100 bases:

>100_bases
GTCAGATTCATTTGTACCAACTTTTGCTGAGTTACTGTTGAAAGTTGGGTATTCTACCCTTATATAGCCCTTGTGTAACT
CTCATTGTCGCGGATTTTTA

Downstream 100 bases:

>100_bases
TACCCTAACGCTTAGCTTCGACAGACTTTAAAGAGGTCTATTAATTTGAGTGATATGGCAAAAAATCTCATTCTCTGGGT
CGTCATCGCCGTTGTGCTGA

Product: 23S rRNA methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MTAKKRSASSTRWMQEHFDDHYVKLAQKRGLRSRAAFKLEELQEKDHLIKPGMTVVDLGAAPGGWSQVAVKLVGDRGKVI
ACDILPMDPIVGVDFLQGDFREDAVLNALLERVGDEKVDVVLSDMAPNMSGSDGVDQPRAMYLVELALDMCHQVLAPNGS
FAVKVFQGEGFDEYMKAVREAFKVVKTRKPDSSRARSREVYLVATGYKL

Sequences:

>Translated_209_residues
MTAKKRSASSTRWMQEHFDDHYVKLAQKRGLRSRAAFKLEELQEKDHLIKPGMTVVDLGAAPGGWSQVAVKLVGDRGKVI
ACDILPMDPIVGVDFLQGDFREDAVLNALLERVGDEKVDVVLSDMAPNMSGSDGVDQPRAMYLVELALDMCHQVLAPNGS
FAVKVFQGEGFDEYMKAVREAFKVVKTRKPDSSRARSREVYLVATGYKL
>Mature_208_residues
TAKKRSASSTRWMQEHFDDHYVKLAQKRGLRSRAAFKLEELQEKDHLIKPGMTVVDLGAAPGGWSQVAVKLVGDRGKVIA
CDILPMDPIVGVDFLQGDFREDAVLNALLERVGDEKVDVVLSDMAPNMSGSDGVDQPRAMYLVELALDMCHQVLAPNGSF
AVKVFQGEGFDEYMKAVREAFKVVKTRKPDSSRARSREVYLVATGYKL

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=214, Percent_Identity=35.981308411215, Blast_Score=140, Evalue=7e-34,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=35.1063829787234, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI29029591, Length=190, Percent_Identity=35.2631578947368, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI29029589, Length=190, Percent_Identity=35.2631578947368, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI7110661, Length=190, Percent_Identity=35.2631578947368, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1789569, Length=208, Percent_Identity=74.5192307692308, Blast_Score=329, Evalue=9e-92,
Organism=Caenorhabditis elegans, GI17553474, Length=220, Percent_Identity=34.0909090909091, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17554650, Length=191, Percent_Identity=34.0314136125654, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71987550, Length=189, Percent_Identity=34.3915343915344, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI71987561, Length=189, Percent_Identity=34.3915343915344, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17553860, Length=189, Percent_Identity=34.3915343915344, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=35.1758793969849, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319796, Length=190, Percent_Identity=33.6842105263158, Blast_Score=105, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321302, Length=96, Percent_Identity=40.625, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356387, Length=221, Percent_Identity=35.2941176470588, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=36.1702127659575, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648639, Length=198, Percent_Identity=33.8383838383838, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24647580, Length=200, Percent_Identity=33, Blast_Score=100, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_SHEAM (A1S454)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926830.1
- ProteinModelPortal:   A1S454
- SMR:   A1S454
- STRING:   A1S454
- GeneID:   4603205
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0953
- NMPDR:   fig|326297.7.peg.894
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   A1S454
- ProtClustDB:   PRK11188
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 23189; Mature: 23058

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: ACT_SITE 164-164 BINDING 63-63 BINDING 65-65 BINDING 83-83 BINDING 99-99 BINDING 124-124

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKKRSASSTRWMQEHFDDHYVKLAQKRGLRSRAAFKLEELQEKDHLIKPGMTVVDLGA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCC
APGGWSQVAVKLVGDRGKVIACDILPMDPIVGVDFLQGDFREDAVLNALLERVGDEKVDV
CCCCHHHHHHEEECCCCCEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCHHHEE
VLSDMAPNMSGSDGVDQPRAMYLVELALDMCHQVLAPNGSFAVKVFQGEGFDEYMKAVRE
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHH
AFKVVKTRKPDSSRARSREVYLVATGYKL
HHHHHHCCCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure 
TAKKRSASSTRWMQEHFDDHYVKLAQKRGLRSRAAFKLEELQEKDHLIKPGMTVVDLGA
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCC
APGGWSQVAVKLVGDRGKVIACDILPMDPIVGVDFLQGDFREDAVLNALLERVGDEKVDV
CCCCHHHHHHEEECCCCCEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCHHHEE
VLSDMAPNMSGSDGVDQPRAMYLVELALDMCHQVLAPNGSFAVKVFQGEGFDEYMKAVRE
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHH
AFKVVKTRKPDSSRARSREVYLVATGYKL
HHHHHHCCCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA