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The map label for this gene is phrB [H]

Identifier: 119773948

GI number: 119773948

Start: 995865

End: 997292

Strand: Direct

Name: phrB [H]

Synonym: Sama_0811

Alternate gene names: 119773948

Gene position: 995865-997292 (Clockwise)

Preceding gene: 119773947

Following gene: 119773949

Centisome position: 23.13

GC content: 56.58

Gene sequence:

>1428_bases
ATGAATACACTTGTCTGGTTCAGGCAGGATCTTCGCACCATAGATAACCAGGCGCTGACCCGGGCGTGTGAAGATGCCAA
CGCCCGTGGTGGCAAGGTGCATGCGCTCTTTATCGCCAGTCCGGGCCAGTGGCAACGGCATGACATGGGTGTGCGTCAGC
AGCGATTTTTACTGGCTAATCTCAATGCGTTGGGTGAAGAGCTTGCCAGTATGGGGATTCCGCTGTCGTTGCTGAAGGTG
GATTTTTTTTATCAAACCGCAGCGGCCCTCAAGGCGTTTTGTAAAGAACACGGTATCTCCAGCATCTTCGCCGGGGCGCA
AATCGAGATTGATGAGCGCAGGCGCGATCAGGCACTTCTGGATAAAGGCTTCCCGCTGCAACTGACCGATGAGCACTGCA
TGCTGCCCCCCGGCAGTGTGGTCACTCAGGGACAGCGGGATCAGGGAGGCGCCATGTACCGGGTATTTACCCCATTTGCC
CGTCGCTGGCGTGAAATAGCCGCTTCCCGCTCGCTTTGGCCTATTCCTGCGCCCGCGTCTGTGGCAACGCCGGTGGTATT
CACCCCCTTCGCGTTTTGGGGAGATATTAACGATGATCCTGCGCTTGATAAATGGCCTGTTGGGCAGGATGCGGCATTGG
AAAAACTATCGCGTTTTCGAGAGCTTGGCATGGGCCGCTACGGCGAGAATCGCGACTTCCCGGCCATTGACGGCACCAGC
AGTCTGTCGCCCTATCTGGCGCTCGGGGTGATATCTCCCCGACAGTGTCTGGCGGCGCTGCTGGAAGACTTTCCCGACGT
CTTTGTGAATGAATCCTCACCGGCCCGTCCTTGGCTGAATGAGTTGGTGTGGCGCGAATTTTACCGGCATCTGCTGGTGG
CTTTCCCGGGTCTGAGCATGGCAAAGAACTTTAACCCCCTCGGTGACGGCATTCGCTGGCGCAATGATGAAACAGAATTT
GAGGCCTGGAAACACGGCCGTACCGGCTATCCGCTGGTGGATGCTGCAATGCGACAACTTAATCAAACCGGCTGGATGCA
CAACCGGCTCAGGATGGTGGTTGCCAGCTTTCTAACCAAGCATCTGCTTATCGACTGGCGCTGGGGTGAGCGATATTTTC
GCGAGCAGCTCATCGATGGTTGCCTTGCGGCTAACAACGGTGGTTGGCAGTGGGCCGCCGGCACAGGTTGTGATGCCCAG
CCTTACTTTCGGATCTTTAACCCTATGAGTCAGTCGGAAAAATTTGATCCCGAAGGACGCTTTATCCGTAAGTACCTACC
AGAATTAGCGCAGTGGCCGACCAGGTCATTGCACCAGGCAAGTGGGCCGGGGCTGTTTGATTCCAGCGGCTATCCGTCTC
CGATTGTTGAGCACACCCAAGCCCGAGTCAGGGCGCTGGATGTGATGGCCGTGATGAAGAAGGGTTAG

Upstream 100 bases:

>100_bases
AAACGACTACATCGCCGCTCAGGTGTATCTGAATCCCCACCCGGATTCGCTGCGTTTGCGATATGCTTACTGACAAGATA
CTGAAAAGCGGAATAAAAAT

Downstream 100 bases:

>100_bases
GGAATGAATGCAGGTGTGCACAGTCCAGTGGAACATCATCCCCTCGGACAAGGTGAGATGGGACATAACCCATTTATTGT
TGCAGCGTCGGCGCCCATTG

Product: deoxyribodipyrimidine photo-lyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNTLVWFRQDLRTIDNQALTRACEDANARGGKVHALFIASPGQWQRHDMGVRQQRFLLANLNALGEELASMGIPLSLLKV
DFFYQTAAALKAFCKEHGISSIFAGAQIEIDERRRDQALLDKGFPLQLTDEHCMLPPGSVVTQGQRDQGGAMYRVFTPFA
RRWREIAASRSLWPIPAPASVATPVVFTPFAFWGDINDDPALDKWPVGQDAALEKLSRFRELGMGRYGENRDFPAIDGTS
SLSPYLALGVISPRQCLAALLEDFPDVFVNESSPARPWLNELVWREFYRHLLVAFPGLSMAKNFNPLGDGIRWRNDETEF
EAWKHGRTGYPLVDAAMRQLNQTGWMHNRLRMVVASFLTKHLLIDWRWGERYFREQLIDGCLAANNGGWQWAAGTGCDAQ
PYFRIFNPMSQSEKFDPEGRFIRKYLPELAQWPTRSLHQASGPGLFDSSGYPSPIVEHTQARVRALDVMAVMKKG

Sequences:

>Translated_475_residues
MNTLVWFRQDLRTIDNQALTRACEDANARGGKVHALFIASPGQWQRHDMGVRQQRFLLANLNALGEELASMGIPLSLLKV
DFFYQTAAALKAFCKEHGISSIFAGAQIEIDERRRDQALLDKGFPLQLTDEHCMLPPGSVVTQGQRDQGGAMYRVFTPFA
RRWREIAASRSLWPIPAPASVATPVVFTPFAFWGDINDDPALDKWPVGQDAALEKLSRFRELGMGRYGENRDFPAIDGTS
SLSPYLALGVISPRQCLAALLEDFPDVFVNESSPARPWLNELVWREFYRHLLVAFPGLSMAKNFNPLGDGIRWRNDETEF
EAWKHGRTGYPLVDAAMRQLNQTGWMHNRLRMVVASFLTKHLLIDWRWGERYFREQLIDGCLAANNGGWQWAAGTGCDAQ
PYFRIFNPMSQSEKFDPEGRFIRKYLPELAQWPTRSLHQASGPGLFDSSGYPSPIVEHTQARVRALDVMAVMKKG
>Mature_475_residues
MNTLVWFRQDLRTIDNQALTRACEDANARGGKVHALFIASPGQWQRHDMGVRQQRFLLANLNALGEELASMGIPLSLLKV
DFFYQTAAALKAFCKEHGISSIFAGAQIEIDERRRDQALLDKGFPLQLTDEHCMLPPGSVVTQGQRDQGGAMYRVFTPFA
RRWREIAASRSLWPIPAPASVATPVVFTPFAFWGDINDDPALDKWPVGQDAALEKLSRFRELGMGRYGENRDFPAIDGTS
SLSPYLALGVISPRQCLAALLEDFPDVFVNESSPARPWLNELVWREFYRHLLVAFPGLSMAKNFNPLGDGIRWRNDETEF
EAWKHGRTGYPLVDAAMRQLNQTGWMHNRLRMVVASFLTKHLLIDWRWGERYFREQLIDGCLAANNGGWQWAAGTGCDAQ
PYFRIFNPMSQSEKFDPEGRFIRKYLPELAQWPTRSLHQASGPGLFDSSGYPSPIVEHTQARVRALDVMAVMKKG

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI4758072, Length=507, Percent_Identity=26.6272189349112, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI188536100, Length=498, Percent_Identity=26.5060240963855, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI188536103, Length=282, Percent_Identity=31.2056737588652, Blast_Score=129, Evalue=7e-30,
Organism=Escherichia coli, GI1786926, Length=494, Percent_Identity=46.1538461538462, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324962, Length=500, Percent_Identity=32.4, Blast_Score=248, Evalue=1e-66,
Organism=Drosophila melanogaster, GI17137248, Length=509, Percent_Identity=28.8801571709234, Blast_Score=171, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24585455, Length=509, Percent_Identity=28.8801571709234, Blast_Score=171, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24648152, Length=516, Percent_Identity=24.6124031007752, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 53749; Mature: 53749

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLVWFRQDLRTIDNQALTRACEDANARGGKVHALFIASPGQWQRHDMGVRQQRFLLAN
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHCCHHHHHHHHHH
LNALGEELASMGIPLSLLKVDFFYQTAAALKAFCKEHGISSIFAGAQIEIDERRRDQALL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEHHHHHHHHHH
DKGFPLQLTDEHCMLPPGSVVTQGQRDQGGAMYRVFTPFARRWREIAASRSLWPIPAPAS
CCCCCEEECCCCCCCCCCHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCHH
VATPVVFTPFAFWGDINDDPALDKWPVGQDAALEKLSRFRELGMGRYGENRDFPAIDGTS
HCCCCEECCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SLSPYLALGVISPRQCLAALLEDFPDVFVNESSPARPWLNELVWREFYRHLLVAFPGLSM
CCCCHHHEECCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
AKNFNPLGDGIRWRNDETEFEAWKHGRTGYPLVDAAMRQLNQTGWMHNRLRMVVASFLTK
HHCCCCCCCCCEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
HLLIDWRWGERYFREQLIDGCLAANNGGWQWAAGTGCDAQPYFRIFNPMSQSEKFDPEGR
HHEEECHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHCCCCCCCCCCCCHH
FIRKYLPELAQWPTRSLHQASGPGLFDSSGYPSPIVEHTQARVRALDVMAVMKKG
HHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTLVWFRQDLRTIDNQALTRACEDANARGGKVHALFIASPGQWQRHDMGVRQQRFLLAN
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHCCHHHHHHHHHH
LNALGEELASMGIPLSLLKVDFFYQTAAALKAFCKEHGISSIFAGAQIEIDERRRDQALL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEHHHHHHHHHH
DKGFPLQLTDEHCMLPPGSVVTQGQRDQGGAMYRVFTPFARRWREIAASRSLWPIPAPAS
CCCCCEEECCCCCCCCCCHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCHH
VATPVVFTPFAFWGDINDDPALDKWPVGQDAALEKLSRFRELGMGRYGENRDFPAIDGTS
HCCCCEECCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SLSPYLALGVISPRQCLAALLEDFPDVFVNESSPARPWLNELVWREFYRHLLVAFPGLSM
CCCCHHHEECCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
AKNFNPLGDGIRWRNDETEFEAWKHGRTGYPLVDAAMRQLNQTGWMHNRLRMVVASFLTK
HHCCCCCCCCCEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
HLLIDWRWGERYFREQLIDGCLAANNGGWQWAAGTGCDAQPYFRIFNPMSQSEKFDPEGR
HHEEECHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHCCCCCCCCCCCCHH
FIRKYLPELAQWPTRSLHQASGPGLFDSSGYPSPIVEHTQARVRALDVMAVMKKG
HHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840665; 11677609 [H]