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The map label for this gene is mdh

Identifier: 119773810

GI number: 119773810

Start: 828182

End: 829117

Strand: Direct

Name: mdh

Synonym: Sama_0672

Alternate gene names: 119773810

Gene position: 828182-829117 (Clockwise)

Preceding gene: 119773808

Following gene: 119773811

Centisome position: 19.23

GC content: 55.66

Gene sequence:

>936_bases
ATGAAAGTTGCTGTTCTTGGTGCCGCCGGTGGTATCGGCCAAGCCCTCGCCCTACTCCTGAAAACCCAACTGCCTGCTGG
TTCCAAGCTGTCTCTGTATGACATTGCCCCTGTCACGCCAGGTGTTGCCGTGGATCTGAGCCACATCCCAACTGCCGTTG
AGGTGAAAGGTTTTTGCGGTGAAGACCCAACGCCTGCGCTGGAAGGTGCCGATGTGGTGCTGATTTCTGCCGGTGTGGCC
CGTAAGCCAGGTATGGATCGTTCTGATCTGTTCAACATCAACGCAGGTATCGTCCGTAACCTGATTGAAAAAGTGGCAGC
GACCTGTCCAAAAGCGTTGGTTGGTATCATCACCAACCCCGTAAACACCACCGTGGCCATTGCCGCTGAAGTGCTGAAGA
AAGCTGGTGTTTATGACAAAAACCGTCTGTTCGGTGTAACCACTCTGGACGTTATCCGCGCCGAGACCTTCGTTGCCGAA
GCTAAGGGCGTTGATGTAGCCAGCGTGAAAGTAAACGTGATTGGCGGCCACAGCGGCGTGACCATTCTGCCTCTGCTGTC
TCAAATTGAAGGCGTAAACTTCAGCGATGAAGAAGTGGCTGCTCTGACCAAGCGCATCCAGAACGCCGGTACTGAAGTCG
TTGAAGCCAAGGCCGGTGGCGGCAGTGCGACCCTGTCTATGGGTCAGGCGGCTTTCCGTTTCGGTATGTCTCTTATCCGT
GGTCTGCAGGGCGAAGCCAATGTGGTTGAGTGCGCCTATGTTGACGGCGGCAGCGAGCACGCTGTGTTCTTCGCTCAGCC
AGTGCTGCTGGGCAAAAACGGCGTAGAAAAAGTACTGCCTTATGGCGAGGTGAGCGCGTTCGAAGCCAACGCCCGCGACG
CCATGCTGGACACCCTCAAGGGTGACATCCAGCTGGGTGTAGACTTCGTTAAGTAA

Upstream 100 bases:

>100_bases
GCGACTAAGGGATAATTGAAATTTGTGTGACGATGCTTTAATGTGGCGCCATCATTTGAGGATCTACCTCACAAATTCCG
AGATATAACGGAGAATACCT

Downstream 100 bases:

>100_bases
GTCGTGTCGATGTGAAGAAACGGAGCCTGGTGCTCCGTTTTTTTATGCCTGAAATCAGCGATTGTGTTGCGACACCGATT
TGCTCATATCCCTTGCATTT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVTPGVAVDLSHIPTAVEVKGFCGEDPTPALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLIEKVAATCPKALVGIITNPVNTTVAIAAEVLKKAGVYDKNRLFGVTTLDVIRAETFVAE
AKGVDVASVKVNVIGGHSGVTILPLLSQIEGVNFSDEEVAALTKRIQNAGTEVVEAKAGGGSATLSMGQAAFRFGMSLIR
GLQGEANVVECAYVDGGSEHAVFFAQPVLLGKNGVEKVLPYGEVSAFEANARDAMLDTLKGDIQLGVDFVK

Sequences:

>Translated_311_residues
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVTPGVAVDLSHIPTAVEVKGFCGEDPTPALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLIEKVAATCPKALVGIITNPVNTTVAIAAEVLKKAGVYDKNRLFGVTTLDVIRAETFVAE
AKGVDVASVKVNVIGGHSGVTILPLLSQIEGVNFSDEEVAALTKRIQNAGTEVVEAKAGGGSATLSMGQAAFRFGMSLIR
GLQGEANVVECAYVDGGSEHAVFFAQPVLLGKNGVEKVLPYGEVSAFEANARDAMLDTLKGDIQLGVDFVK
>Mature_311_residues
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVTPGVAVDLSHIPTAVEVKGFCGEDPTPALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLIEKVAATCPKALVGIITNPVNTTVAIAAEVLKKAGVYDKNRLFGVTTLDVIRAETFVAE
AKGVDVASVKVNVIGGHSGVTILPLLSQIEGVNFSDEEVAALTKRIQNAGTEVVEAKAGGGSATLSMGQAAFRFGMSLIR
GLQGEANVVECAYVDGGSEHAVFFAQPVLLGKNGVEKVLPYGEVSAFEANARDAMLDTLKGDIQLGVDFVK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 1 family

Homologues:

Organism=Homo sapiens, GI21735621, Length=312, Percent_Identity=56.4102564102564, Blast_Score=327, Evalue=9e-90,
Organism=Homo sapiens, GI47059044, Length=290, Percent_Identity=24.1379310344828, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI221136809, Length=290, Percent_Identity=24.1379310344828, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI5031857, Length=323, Percent_Identity=25.077399380805, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI260099723, Length=323, Percent_Identity=25.077399380805, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI15082234, Length=317, Percent_Identity=23.3438485804416, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1789632, Length=310, Percent_Identity=80, Blast_Score=495, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17554310, Length=313, Percent_Identity=58.14696485623, Blast_Score=328, Evalue=3e-90,
Organism=Saccharomyces cerevisiae, GI6322765, Length=315, Percent_Identity=51.4285714285714, Blast_Score=282, Evalue=5e-77,
Organism=Saccharomyces cerevisiae, GI6324446, Length=367, Percent_Identity=37.0572207084469, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6320125, Length=345, Percent_Identity=37.1014492753623, Blast_Score=180, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24647881, Length=310, Percent_Identity=57.4193548387097, Blast_Score=331, Evalue=3e-91,
Organism=Drosophila melanogaster, GI24663599, Length=313, Percent_Identity=50.1597444089457, Blast_Score=267, Evalue=6e-72,
Organism=Drosophila melanogaster, GI24663595, Length=300, Percent_Identity=43.6666666666667, Blast_Score=241, Evalue=3e-64,
Organism=Drosophila melanogaster, GI17136226, Length=326, Percent_Identity=25.7668711656442, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_SHEAM (A1S3C4)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926550.1
- ProteinModelPortal:   A1S3C4
- SMR:   A1S3C4
- STRING:   A1S3C4
- GeneID:   4602925
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0672
- NMPDR:   fig|326297.7.peg.627
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   ECTYVEG
- PhylomeDB:   A1S3C4
- ProtClustDB:   PRK05086
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01516
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010097
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11540:SF1
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01772

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 32021; Mature: 32021

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 177-177 BINDING 34-34 BINDING 81-81 BINDING 87-87 BINDING 94-94 BINDING 119-119 BINDING 153-153 BINDING 227-227

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVTPGVAVDLSHIPTAVEVKGFCG
CEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEHHHCCCEEEEECCCC
EDPTPALEGADVVLISAGVARKPGMDRSDLFNINAGIVRNLIEKVAATCPKALVGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCHHHHHHHHCCC
VNTTVAIAAEVLKKAGVYDKNRLFGVTTLDVIRAETFVAEAKGVDVASVKVNVIGGHSGV
CCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCC
TILPLLSQIEGVNFSDEEVAALTKRIQNAGTEVVEAKAGGGSATLSMGQAAFRFGMSLIR
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GLQGEANVVECAYVDGGSEHAVFFAQPVLLGKNGVEKVLPYGEVSAFEANARDAMLDTLK
CCCCCCCEEEEEEECCCCCCEEEEECEEEECCCCHHHHCCCCCCEEECCCCHHHHHHHHC
GDIQLGVDFVK
CCEEECEEECC
>Mature Secondary Structure
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVTPGVAVDLSHIPTAVEVKGFCG
CEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEHHHCCCEEEEECCCC
EDPTPALEGADVVLISAGVARKPGMDRSDLFNINAGIVRNLIEKVAATCPKALVGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCHHHHHHHHCCC
VNTTVAIAAEVLKKAGVYDKNRLFGVTTLDVIRAETFVAEAKGVDVASVKVNVIGGHSGV
CCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCC
TILPLLSQIEGVNFSDEEVAALTKRIQNAGTEVVEAKAGGGSATLSMGQAAFRFGMSLIR
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GLQGEANVVECAYVDGGSEHAVFFAQPVLLGKNGVEKVLPYGEVSAFEANARDAMLDTLK
CCCCCCCEEEEEEECCCCCCEEEEECEEEECCCCHHHHCCCCCCEEECCCCHHHHHHHHC
GDIQLGVDFVK
CCEEECEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA