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The map label for this gene is nrfA

Identifier: 119773786

GI number: 119773786

Start: 803874

End: 805277

Strand: Direct

Name: nrfA

Synonym: Sama_0648

Alternate gene names: 119773786

Gene position: 803874-805277 (Clockwise)

Preceding gene: 119773783

Following gene: 119773788

Centisome position: 18.67

GC content: 54.63

Gene sequence:

>1404_bases
ATGGTGAAAAAGCTAACCGGAAAATCCTTTGCGCTGAGCGCATTGGTCGCCGCCAGCTTTGTAGCTGCCGGTGCGATGGC
CAGTGATAAAACCGAGCCACGCAATGACGTATACAAAGACAAATTCAGCAAACAATATAACAGTTGGCACGCGACTGCCG
AAAGCGAAGCTATCACAGATGCGCTGGAGCAGGATCCTGCACTGGTGATTTTGTGGGCTGGTTACGGTTTTGCCAAAGAC
TACAATGCCCCCCGTGGTCACATGTATGCTCTGACTGACGTGCGTAACACCCTGCGTACCGGTGCTCCAACCTCAGCCGA
AGATGGCCCGATGCCAATGGCTTGCTGGAGCTGTAAGAGCCCTGACGTGCCCCGTCTGATTGAAGAACAGGGCGAATCAG
GCTATTTCACCGGTAAGTGGGCAAAAGGCGGCGCCGAAGTGGCCAACACCATCGGTTGCAGCGACTGTCACGAAAAAGGC
ACCCCTAAACTGCGCCTGTCTCGTCCTTTTGCTTCCCGCGCCATGGAAGCCATTGGTACCCCATTCGACAAGGCTTCCAA
GCAGGACAAAGAATCCATGGTGTGCGCCCAGTGCCACGTGGAGTACTACTTCGAGAAGACTGACGATCGCAAAGGTTTCG
TGAAGTTCCCATGGGACGGCGGCACCACAGTGGAAAACATGGAAGTCTATTATGACGCCATCCAGTTTGCCGACTGGACC
CACGCTGTGTCCAAAACCCCAATGCTGAAAGCGCAGCACCCAGGTTATGAAACCTGGAAACTGGGTACTCACGGTCAAAA
CAACGTGTCCTGTGTTGACTGTCACATGCCGAAAGTCACCAATGAACAGGGCAAGAAGTTTACCGATCACAAAGTGGGCA
ACCCCTTCGATCGTTTCGAAGAAACTTGCGGTACCTGCCACAGTCAGGACAAAGAACACATGCTGACCGTCTACAAGGAC
AACAAGTCCAAGGTGATGGAGCTCAAGTCCAAGGCTGAAGCTCAGCTGGTTGCTGCTCACTTCGAAGCCGGTGCTGCCTG
GAAAGCCGGTGCCACCGAAGATGAAATGAAGCCTATCCTGACCAACATCCGTCACGCTCAGTGGCGCTGGGACTACGCCA
TTGCCTCTCACGGCGTGTCTGCACATGCTCCAGCTGAAGCACTGCGTGTACTGGGCACTGCCGTAGACAAGGCTGCCAAC
GCCCGCGTTCAACTGGCTCAGTTGCTGGCCACCAAAGGCGTGAAGCAGCCTATCGAGCTGCCGGACATCTCTACCAAGGC
CAAAGCTCAGGCTGCTCTGGGTATGGACATGGACAAGATGAACGCCGATAAGGCCAAGTTCAAGCAAGAAATGCTGCCCA
AGTGGGAAGCAGACGCCAAGGCCCGCGAAGCCACTTACAAGTAA

Upstream 100 bases:

>100_bases
ACCACTTAATGGGTATGTAAAAGGAGGGTTAACAGGACTACTTTTGACCCTGGAGATGTCGTGGGTATTTCAGAAACACA
AAAACTAACACGGAGATGAG

Downstream 100 bases:

>100_bases
GCAGCAAACCCCAAGCCTGCATACACAAAGCCCCCGCAATGCGGGGGCTTTTCTTTGGCAGACGTGAGTAATAGAGCGGC
TTATTTGATGAGTTTCAGCA

Product: cytochrome c552

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MVKKLTGKSFALSALVAASFVAAGAMASDKTEPRNDVYKDKFSKQYNSWHATAESEAITDALEQDPALVILWAGYGFAKD
YNAPRGHMYALTDVRNTLRTGAPTSAEDGPMPMACWSCKSPDVPRLIEEQGESGYFTGKWAKGGAEVANTIGCSDCHEKG
TPKLRLSRPFASRAMEAIGTPFDKASKQDKESMVCAQCHVEYYFEKTDDRKGFVKFPWDGGTTVENMEVYYDAIQFADWT
HAVSKTPMLKAQHPGYETWKLGTHGQNNVSCVDCHMPKVTNEQGKKFTDHKVGNPFDRFEETCGTCHSQDKEHMLTVYKD
NKSKVMELKSKAEAQLVAAHFEAGAAWKAGATEDEMKPILTNIRHAQWRWDYAIASHGVSAHAPAEALRVLGTAVDKAAN
ARVQLAQLLATKGVKQPIELPDISTKAKAQAALGMDMDKMNADKAKFKQEMLPKWEADAKAREATYK

Sequences:

>Translated_467_residues
MVKKLTGKSFALSALVAASFVAAGAMASDKTEPRNDVYKDKFSKQYNSWHATAESEAITDALEQDPALVILWAGYGFAKD
YNAPRGHMYALTDVRNTLRTGAPTSAEDGPMPMACWSCKSPDVPRLIEEQGESGYFTGKWAKGGAEVANTIGCSDCHEKG
TPKLRLSRPFASRAMEAIGTPFDKASKQDKESMVCAQCHVEYYFEKTDDRKGFVKFPWDGGTTVENMEVYYDAIQFADWT
HAVSKTPMLKAQHPGYETWKLGTHGQNNVSCVDCHMPKVTNEQGKKFTDHKVGNPFDRFEETCGTCHSQDKEHMLTVYKD
NKSKVMELKSKAEAQLVAAHFEAGAAWKAGATEDEMKPILTNIRHAQWRWDYAIASHGVSAHAPAEALRVLGTAVDKAAN
ARVQLAQLLATKGVKQPIELPDISTKAKAQAALGMDMDKMNADKAKFKQEMLPKWEADAKAREATYK
>Mature_467_residues
MVKKLTGKSFALSALVAASFVAAGAMASDKTEPRNDVYKDKFSKQYNSWHATAESEAITDALEQDPALVILWAGYGFAKD
YNAPRGHMYALTDVRNTLRTGAPTSAEDGPMPMACWSCKSPDVPRLIEEQGESGYFTGKWAKGGAEVANTIGCSDCHEKG
TPKLRLSRPFASRAMEAIGTPFDKASKQDKESMVCAQCHVEYYFEKTDDRKGFVKFPWDGGTTVENMEVYYDAIQFADWT
HAVSKTPMLKAQHPGYETWKLGTHGQNNVSCVDCHMPKVTNEQGKKFTDHKVGNPFDRFEETCGTCHSQDKEHMLTVYKD
NKSKVMELKSKAEAQLVAAHFEAGAAWKAGATEDEMKPILTNIRHAQWRWDYAIASHGVSAHAPAEALRVLGTAVDKAAN
ARVQLAQLLATKGVKQPIELPDISTKAKAQAALGMDMDKMNADKAKFKQEMLPKWEADAKAREATYK

Specific function: Plays a role in nitrite reduction

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family

Homologues:

Organism=Escherichia coli, GI1790506, Length=437, Percent_Identity=61.3272311212815, Blast_Score=586, Evalue=1e-168,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NRFA_SHEAM (A1S3A0)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926526.1
- ProteinModelPortal:   A1S3A0
- SMR:   A1S3A0
- STRING:   A1S3A0
- GeneID:   4602901
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0648
- NMPDR:   fig|326297.7.peg.607
- eggNOG:   COG3303
- HOGENOM:   HBG488281
- OMA:   PWDMGTT
- PhylomeDB:   A1S3A0
- ProtClustDB:   PRK11125
- GO:   GO:0006810
- HAMAP:   MF_01182
- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031
- PIRSF:   PIRSF000243
- TIGRFAMs:   TIGR03152

Pfam domain/function: PF02335 Cytochrom_C552

EC number: =1.7.2.2

Molecular weight: Translated: 51569; Mature: 51569

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: BINDING 115-115 BINDING 118-118 BINDING 153-153 BINDING 156-156 BINDING 195-195 BINDING 198-198 BINDING 202-202 BINDING 253-253 BINDING 271-271 BINDING 274-274 BINDING 303-303 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKLTGKSFALSALVAASFVAAGAMASDKTEPRNDVYKDKFSKQYNSWHATAESEAITD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
ALEQDPALVILWAGYGFAKDYNAPRGHMYALTDVRNTLRTGAPTSAEDGPMPMACWSCKS
HHHCCCCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCEEEECCCC
PDVPRLIEEQGESGYFTGKWAKGGAEVANTIGCSDCHEKGTPKLRLSRPFASRAMEAIGT
CCCCHHHHHCCCCCEEECCCCCCCHHHHHHCCCCHHHHCCCCCEEECCCHHHHHHHHHCC
PFDKASKQDKESMVCAQCHVEYYFEKTDDRKGFVKFPWDGGTTVENMEVYYDAIQFADWT
CHHHHCCHHHHHHHHHHHHHHHEEECCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHH
HAVSKTPMLKAQHPGYETWKLGTHGQNNVSCVDCHMPKVTNEQGKKFTDHKVGNPFDRFE
HHHCCCCCEEECCCCCCEEECCCCCCCCCEEEECCCCCCCCCCCCEECCCCCCCHHHHHH
ETCGTCHSQDKEHMLTVYKDNKSKVMELKSKAEAQLVAAHFEAGAAWKAGATEDEMKPIL
HHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
TNIRHAQWRWDYAIASHGVSAHAPAEALRVLGTAVDKAANARVQLAQLLATKGVKQPIEL
HHHHHHHEEEHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PDISTKAKAQAALGMDMDKMNADKAKFKQEMLPKWEADAKAREATYK
CCCCCHHHHHHHHCCCHHHCCCHHHHHHHHHCCCCCCCCHHHHCCCC
>Mature Secondary Structure
MVKKLTGKSFALSALVAASFVAAGAMASDKTEPRNDVYKDKFSKQYNSWHATAESEAITD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
ALEQDPALVILWAGYGFAKDYNAPRGHMYALTDVRNTLRTGAPTSAEDGPMPMACWSCKS
HHHCCCCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCEEEECCCC
PDVPRLIEEQGESGYFTGKWAKGGAEVANTIGCSDCHEKGTPKLRLSRPFASRAMEAIGT
CCCCHHHHHCCCCCEEECCCCCCCHHHHHHCCCCHHHHCCCCCEEECCCHHHHHHHHHCC
PFDKASKQDKESMVCAQCHVEYYFEKTDDRKGFVKFPWDGGTTVENMEVYYDAIQFADWT
CHHHHCCHHHHHHHHHHHHHHHEEECCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHH
HAVSKTPMLKAQHPGYETWKLGTHGQNNVSCVDCHMPKVTNEQGKKFTDHKVGNPFDRFE
HHHCCCCCEEECCCCCCEEECCCCCCCCCEEEECCCCCCCCCCCCEECCCCCCCHHHHHH
ETCGTCHSQDKEHMLTVYKDNKSKVMELKSKAEAQLVAAHFEAGAAWKAGATEDEMKPIL
HHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
TNIRHAQWRWDYAIASHGVSAHAPAEALRVLGTAVDKAANARVQLAQLLATKGVKQPIEL
HHHHHHHEEEHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PDISTKAKAQAALGMDMDKMNADKAKFKQEMLPKWEADAKAREATYK
CCCCCHHHHHHHHCCCHHHCCCHHHHHHHHHCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA