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The map label for this gene is rstB [H]

Identifier: 119773297

GI number: 119773297

Start: 180750

End: 181979

Strand: Direct

Name: rstB [H]

Synonym: Sama_0155

Alternate gene names: 119773297

Gene position: 180750-181979 (Clockwise)

Preceding gene: 119773296

Following gene: 119773298

Centisome position: 4.2

GC content: 55.04

Gene sequence:

>1230_bases
ATGTTGAAGCTTTACAGTGGTCTGCTATTGGCAGTCGTGAGTTCGCTACTGTTGTTCTCGCGGATTTACGATATCAGCAC
CCAGCAGCCACACCTGCTCTATCCCCAGGAAGAGGCCTTTTTAAACCAGCTGTTTGCCCTGACCGAAGGCAGTATGGATT
TGTCGCAGCTGCCGGTCGCCAGCCTGCCCCTGGAGCAGCTGGCCATTCCCGATGAGACCCGGGCTCTGCTGCTGCAGGGT
GGCACAGCAGTGCTGCAGGATAAGGCGCAGGCGTTTTATTTTTACCGGCAGAACCCCGCCGACCCCAGCCAGGTGACCAT
TGTTGGTCCTATCGATTACCCTGAAAGCCCTCTTGACCGGCAACCCGTGCTCTGGCAACTGCTGCCCTATGCTTTGCTTG
CATTGGCGCTACTGTTGATTTGCTGGCCACTGTATTTGGATATCAAGAAGTTGAAGGCATTTTGCGCCCAGGTCAGCCGG
GTTGATTTTGGGGCCACTGCGTCCATCACCCGACTAACCATGTTGGGAGCCATCGGCAATACCATGGCAGACATGGCGCA
AAAGCTCGCCTTTCACCGCAGTCAACAGCGGGACTTTATCAATGCCGTCTCCCATGATTTTCTGACTCCCCTGGCCAGAA
TGAAATTTACCCTGGTAATGGGGCGTAGCGCCCATAGTCAGGCAGTCTCTGATACTTTCGAGAGTCTCGATGAAGATTTG
GCTGAGCTTGAGCAACTTATTGACGAGTTTTTAAGCCATGCCGAGTTGTCTCAGTATCGGCCGAGCGAGCAGCAACAAAG
CTTCTCTGGCAGACAGCTGGTCGCCGACTGCTTTGCCAAGTTTGAACCGCTGGTGAGTGTGACTCTGGAAATAGCGGGAG
AGGCCGATGTGATAGTGCAGGATAAGGCCGGATTTGAGCGCATCATGCAAAACCTCATCAGCAATGCCTCCCGCTATGCC
AGTTCTCGGGTGAGGGTCAGCCTGAACTGGGATAAGGAGCTGGTGGTGCAGGTGGAAGATGACGGCCCTGGCTTCACCGA
AAATACAGATTCGCTGCTTGCTGCCTTTTATCAGGACGAACTTTCTACGCATCGACTCTATCAGGGCGTGGGCCTGGGCT
TGTCGACAGTCGCTCTGCTTTGCCGCCATCTGTCTGTTGAAGTGACCCTGGGACGCAGTCAGGCACTGGGTGGTGCCTGC
GTTACCCTGAAATGCCCCTCAGCCCCCTGA

Upstream 100 bases:

>100_bases
GCCTCAGACGCAAGCTGGGGGATAACATGGACGATCCCTTTAAAATCAAAACTATCTGGGGTAAGGGTTACCTGTTTGTC
CCGGATATTTGGGGCTAGGC

Downstream 100 bases:

>100_bases
ATTCGAGCGCCTTGTACTTTACGTTGATTTTCATCGCGCCAGCATCAGGCCCTCATTCGTGACGAGCAAGGTGTGTTAAT
TAAGTTTTTGAATTTAGGTT

Product: signal transduction histidine kinase-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MLKLYSGLLLAVVSSLLLFSRIYDISTQQPHLLYPQEEAFLNQLFALTEGSMDLSQLPVASLPLEQLAIPDETRALLLQG
GTAVLQDKAQAFYFYRQNPADPSQVTIVGPIDYPESPLDRQPVLWQLLPYALLALALLLICWPLYLDIKKLKAFCAQVSR
VDFGATASITRLTMLGAIGNTMADMAQKLAFHRSQQRDFINAVSHDFLTPLARMKFTLVMGRSAHSQAVSDTFESLDEDL
AELEQLIDEFLSHAELSQYRPSEQQQSFSGRQLVADCFAKFEPLVSVTLEIAGEADVIVQDKAGFERIMQNLISNASRYA
SSRVRVSLNWDKELVVQVEDDGPGFTENTDSLLAAFYQDELSTHRLYQGVGLGLSTVALLCRHLSVEVTLGRSQALGGAC
VTLKCPSAP

Sequences:

>Translated_409_residues
MLKLYSGLLLAVVSSLLLFSRIYDISTQQPHLLYPQEEAFLNQLFALTEGSMDLSQLPVASLPLEQLAIPDETRALLLQG
GTAVLQDKAQAFYFYRQNPADPSQVTIVGPIDYPESPLDRQPVLWQLLPYALLALALLLICWPLYLDIKKLKAFCAQVSR
VDFGATASITRLTMLGAIGNTMADMAQKLAFHRSQQRDFINAVSHDFLTPLARMKFTLVMGRSAHSQAVSDTFESLDEDL
AELEQLIDEFLSHAELSQYRPSEQQQSFSGRQLVADCFAKFEPLVSVTLEIAGEADVIVQDKAGFERIMQNLISNASRYA
SSRVRVSLNWDKELVVQVEDDGPGFTENTDSLLAAFYQDELSTHRLYQGVGLGLSTVALLCRHLSVEVTLGRSQALGGAC
VTLKCPSAP
>Mature_409_residues
MLKLYSGLLLAVVSSLLLFSRIYDISTQQPHLLYPQEEAFLNQLFALTEGSMDLSQLPVASLPLEQLAIPDETRALLLQG
GTAVLQDKAQAFYFYRQNPADPSQVTIVGPIDYPESPLDRQPVLWQLLPYALLALALLLICWPLYLDIKKLKAFCAQVSR
VDFGATASITRLTMLGAIGNTMADMAQKLAFHRSQQRDFINAVSHDFLTPLARMKFTLVMGRSAHSQAVSDTFESLDEDL
AELEQLIDEFLSHAELSQYRPSEQQQSFSGRQLVADCFAKFEPLVSVTLEIAGEADVIVQDKAGFERIMQNLISNASRYA
SSRVRVSLNWDKELVVQVEDDGPGFTENTDSLLAAFYQDELSTHRLYQGVGLGLSTVALLCRHLSVEVTLGRSQALGGAC
VTLKCPSAP

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=374, Percent_Identity=27.807486631016, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789808, Length=231, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790346, Length=208, Percent_Identity=24.0384615384615, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788393, Length=278, Percent_Identity=23.7410071942446, Blast_Score=62, Evalue=8e-11,
Organism=Escherichia coli, GI1790551, Length=213, Percent_Identity=26.2910798122066, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 45224; Mature: 45224

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLYSGLLLAVVSSLLLFSRIYDISTQQPHLLYPQEEAFLNQLFALTEGSMDLSQLPVA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHCCCCCHHHCCCH
SLPLEQLAIPDETRALLLQGGTAVLQDKAQAFYFYRQNPADPSQVTIVGPIDYPESPLDR
HCCHHHHCCCCHHHEEEEECCHHHHHHHHHEEEEEECCCCCCCCEEEEECCCCCCCCCCC
QPVLWQLLPYALLALALLLICWPLYLDIKKLKAFCAQVSRVDFGATASITRLTMLGAIGN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TMADMAQKLAFHRSQQRDFINAVSHDFLTPLARMKFTLVMGRSAHSQAVSDTFESLDEDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHH
AELEQLIDEFLSHAELSQYRPSEQQQSFSGRQLVADCFAKFEPLVSVTLEIAGEADVIVQ
HHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHEEECCCCCEEEE
DKAGFERIMQNLISNASRYASSRVRVSLNWDKELVVQVEDDGPGFTENTDSLLAAFYQDE
CCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHH
LSTHRLYQGVGLGLSTVALLCRHLSVEVTLGRSQALGGACVTLKCPSAP
HHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MLKLYSGLLLAVVSSLLLFSRIYDISTQQPHLLYPQEEAFLNQLFALTEGSMDLSQLPVA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHCCCCCHHHCCCH
SLPLEQLAIPDETRALLLQGGTAVLQDKAQAFYFYRQNPADPSQVTIVGPIDYPESPLDR
HCCHHHHCCCCHHHEEEEECCHHHHHHHHHEEEEEECCCCCCCCEEEEECCCCCCCCCCC
QPVLWQLLPYALLALALLLICWPLYLDIKKLKAFCAQVSRVDFGATASITRLTMLGAIGN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TMADMAQKLAFHRSQQRDFINAVSHDFLTPLARMKFTLVMGRSAHSQAVSDTFESLDEDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHH
AELEQLIDEFLSHAELSQYRPSEQQQSFSGRQLVADCFAKFEPLVSVTLEIAGEADVIVQ
HHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHEEECCCCCEEEE
DKAGFERIMQNLISNASRYASSRVRVSLNWDKELVVQVEDDGPGFTENTDSLLAAFYQDE
CCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHH
LSTHRLYQGVGLGLSTVALLCRHLSVEVTLGRSQALGGACVTLKCPSAP
HHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]