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The map label for this gene is hlb [H]

Identifier: 119773257

GI number: 119773257

Start: 129110

End: 130114

Strand: Direct

Name: hlb [H]

Synonym: Sama_0115

Alternate gene names: 119773257

Gene position: 129110-130114 (Clockwise)

Preceding gene: 119773256

Following gene: 119773267

Centisome position: 3.0

GC content: 50.75

Gene sequence:

>1005_bases
ATGAGAAAGCATCATGCACTGCTAATTACCCTGATTTTTGCCCTGCAACCTCAAGCCTATGCCGAATCACTTAAAGTGAT
GGCGTACAACATTATGCAGCTCAATGTGCAGGATTGGGATCAAGCCAATCGTGCTGCGCGATTGCCTGCCGCACTGACAC
AACTGTCTGAACAGCCCGACGTCATTCTGGTGAGTGAAGCCTTTAACGATGAGGCTGAAACCGCGCTGAGTTCCCTTGCC
AGTCTATACCCATATCAAACCGCCAATGTGGGTGAAAACTGCAGCGGCGCTGATTGGGATGCACTGACCGGAAGCTGCTC
CAACTCCCCCATGGTGGTAAGGGGGGGCGTTGTTATCCTGTCTAAATACCCGATATTGGTGCGCAAGGCCCATGTGTTTA
ATCACAGTTTGCAGGGTAGCTGGGATGACTATGCCAACAAGGGGTTTGCCTATGTCGCCATCGAGAAAAACGGCGAGCGA
TTTCACCTGATTGGCACTCACTTGCAGGCAACCCACGACGATGACACAGCGCAAGAGCATCAGGTTCGTCTGGGGCAACT
GGGCGAAATTCAGGCCTTTATCGAGGCTGAGCAGATCCCGGCAAATGAGGCTGTCATTATCGGCGGTGATATGAATGTGG
AATGGAGCAAACAACAGGAAGTGACCGATATGCTGGCCACCGTCAATGCCGGGCTGAATTTTTCTACTCCGGTAACAGGG
TCTTTCAGCGCCAGGGATAACTGGTTTACCAAGGCAAATGCCTACTACTTTGACTACAGCCTCGACTACAACGATACCCT
CGACTACGTATTCTGGCATCAGGACCACAAGCAGCCGGCCAATAATCCGGAAATGGCTGTGCGTTATCCCAAGGCACAAA
TCAGTTGGTATTGGTGGTATCTGAAAGGCTATTGGAACTTGAGTTCGGGCCGGTATTATCACAATGGATATTACAATGAG
CTGTCGGATCACTATCCGGTACAGGTGAACTTCGAGTTCAACTGA

Upstream 100 bases:

>100_bases
TGACGTCTTAATGACATTCAGCTGTGTTCCAGTATGGGCCGCTACCTGAGTAACTGAGTAGCACTCATATAATTCATAAC
CACAAACTGGAGATGCCTGG

Downstream 100 bases:

>100_bases
GACACCAAATGAAAAAGCCCCGACCAGGGGCTTTGATGGTGTGGCTACTGTCCTGTAAAGGAGAGCCTAATGGGCCAGTA
TCAGTACAGTGCCGGCCACC

Product: phospholipase C

Products: NA

Alternate protein names: Beta-hemolysin; Beta-toxin; Sphingomyelinase; SMase [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MRKHHALLITLIFALQPQAYAESLKVMAYNIMQLNVQDWDQANRAARLPAALTQLSEQPDVILVSEAFNDEAETALSSLA
SLYPYQTANVGENCSGADWDALTGSCSNSPMVVRGGVVILSKYPILVRKAHVFNHSLQGSWDDYANKGFAYVAIEKNGER
FHLIGTHLQATHDDDTAQEHQVRLGQLGEIQAFIEAEQIPANEAVIIGGDMNVEWSKQQEVTDMLATVNAGLNFSTPVTG
SFSARDNWFTKANAYYFDYSLDYNDTLDYVFWHQDHKQPANNPEMAVRYPKAQISWYWWYLKGYWNLSSGRYYHNGYYNE
LSDHYPVQVNFEFN

Sequences:

>Translated_334_residues
MRKHHALLITLIFALQPQAYAESLKVMAYNIMQLNVQDWDQANRAARLPAALTQLSEQPDVILVSEAFNDEAETALSSLA
SLYPYQTANVGENCSGADWDALTGSCSNSPMVVRGGVVILSKYPILVRKAHVFNHSLQGSWDDYANKGFAYVAIEKNGER
FHLIGTHLQATHDDDTAQEHQVRLGQLGEIQAFIEAEQIPANEAVIIGGDMNVEWSKQQEVTDMLATVNAGLNFSTPVTG
SFSARDNWFTKANAYYFDYSLDYNDTLDYVFWHQDHKQPANNPEMAVRYPKAQISWYWWYLKGYWNLSSGRYYHNGYYNE
LSDHYPVQVNFEFN
>Mature_334_residues
MRKHHALLITLIFALQPQAYAESLKVMAYNIMQLNVQDWDQANRAARLPAALTQLSEQPDVILVSEAFNDEAETALSSLA
SLYPYQTANVGENCSGADWDALTGSCSNSPMVVRGGVVILSKYPILVRKAHVFNHSLQGSWDDYANKGFAYVAIEKNGER
FHLIGTHLQATHDDDTAQEHQVRLGQLGEIQAFIEAEQIPANEAVIIGGDMNVEWSKQQEVTDMLATVNAGLNFSTPVTG
SFSARDNWFTKANAYYFDYSLDYNDTLDYVFWHQDHKQPANNPEMAVRYPKAQISWYWWYLKGYWNLSSGRYYHNGYYNE
LSDHYPVQVNFEFN

Specific function: Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Beta-hemolysin is a phospholipase C with specific activity toward sphingomyelins. Has a high specificity for sphingomyelin, hydrolyzes lysophosphatidylcholine at a

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the neutral sphingomyelinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005135
- InterPro:   IPR017766 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.4.3 [H]

Molecular weight: Translated: 37962; Mature: 37962

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKHHALLITLIFALQPQAYAESLKVMAYNIMQLNVQDWDQANRAARLPAALTQLSEQPD
CCCHHHHHEEHHHHCCCHHHHHHHHEEEEEEEEEECCCHHHCCHHHCCHHHHHHHCCCCC
VILVSEAFNDEAETALSSLASLYPYQTANVGENCSGADWDALTGSCSNSPMVVRGGVVIL
EEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEE
SKYPILVRKAHVFNHSLQGSWDDYANKGFAYVAIEKNGERFHLIGTHLQATHDDDTAQEH
ECCCEEEEEEHHHCCCCCCCCHHHCCCCEEEEEEECCCCEEEEEEEEEEECCCCCCHHHH
QVRLGQLGEIQAFIEAEQIPANEAVIIGGDMNVEWSKQQEVTDMLATVNAGLNFSTPVTG
HHHHCCCCCEEEEEEHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
SFSARDNWFTKANAYYFDYSLDYNDTLDYVFWHQDHKQPANNPEMAVRYPKAQISWYWWY
CCCCCCCCEEECCEEEEEEEECCCCCEEEEEEECCCCCCCCCCCEEEEECCCEEEEEEEE
LKGYWNLSSGRYYHNGYYNELSDHYPVQVNFEFN
EEEEEECCCCEEEECCCCCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MRKHHALLITLIFALQPQAYAESLKVMAYNIMQLNVQDWDQANRAARLPAALTQLSEQPD
CCCHHHHHEEHHHHCCCHHHHHHHHEEEEEEEEEECCCHHHCCHHHCCHHHHHHHCCCCC
VILVSEAFNDEAETALSSLASLYPYQTANVGENCSGADWDALTGSCSNSPMVVRGGVVIL
EEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEE
SKYPILVRKAHVFNHSLQGSWDDYANKGFAYVAIEKNGERFHLIGTHLQATHDDDTAQEH
ECCCEEEEEEHHHCCCCCCCCHHHCCCCEEEEEEECCCCEEEEEEEEEEECCCCCCHHHH
QVRLGQLGEIQAFIEAEQIPANEAVIIGGDMNVEWSKQQEVTDMLATVNAGLNFSTPVTG
HHHHCCCCCEEEEEEHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
SFSARDNWFTKANAYYFDYSLDYNDTLDYVFWHQDHKQPANNPEMAVRYPKAQISWYWWY
CCCCCCCCEEECCEEEEEEEECCCCCEEEEEEECCCCCCCCCCCEEEEECCCEEEEEEEE
LKGYWNLSSGRYYHNGYYNELSDHYPVQVNFEFN
EEEEEECCCCEEEECCCCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8914839 [H]