Click here to switch to the map view.

The map label for this gene is hutC [H]

Identifier: 119773223

GI number: 119773223

Start: 89362

End: 90066

Strand: Direct

Name: hutC [H]

Synonym: Sama_0081

Alternate gene names: 119773223

Gene position: 89362-90066 (Clockwise)

Preceding gene: 119773220

Following gene: 119773224

Centisome position: 2.08

GC content: 56.17

Gene sequence:

>705_bases
ATGGCCACAGCCAAGTTTGCCGAAATCAAAGAATACATACGGCGCCACATTGAAGCGGGCGAATGGGAAGAAAATACCCG
GGTGCCCTCGGAGAACCAGCTCGCCGAGCAGTTTGGCTGCAGCCGTATGACCGCCCGCCGCGCGCTGACTGAATTGGTTG
AGGCAGGGGTGTTGGAGCGCTCTCAGGGGCTAGGTACCTTTGTGGCGTCACTCAAGTCCCAGTCAAGCATGTTGTCTATT
CGCAATATCGCTGATGAAATCAAGGGGCGCGGTCATGGCCACAGTGTGCAGGTGGTGGAATTGGCCGCTATCAATGCCAT
CCCCCCGATCGCTATTGCCCTTGGGCTGGAGGAGGGCACTGAAGTGTTTTACTCATTGCTGGTGCATTGCGAACAGGGAA
TACCCCTGCAACTGGAGGAGCGTTTTGTTAATCCCAGTCTGGCGCCCGGGTATCTTAGGCAGGATTTCAGCCAACTGACA
CCCCATGAGTATCTGTCGCACGTCGCCCCGCTGACGGAGGCGCGTCATACCATAGAGGCGATTATGCCTAAAGAGCAGAT
CCGGAACAGCCTGAATATACCCGAAGGCGAACCCTGCCTGCAGATCATCCGTCGTACCTGGTCCCGTCAGGGGGTAGTGA
GTTTTGCCCGCTTGGTTCATCCCGGCAGTCGTTTTCGTCTTGGTGGCCACCTTACCTTTAAATAG

Upstream 100 bases:

>100_bases
TCAGCCAGTATCTACTTGTATATACAAGTTTGGATTATAGCCGACACTCAGGCGGGAAAGACAGCCGCAAACCGTTACCG
GCGGAAATTAAGGGACCCAA

Downstream 100 bases:

>100_bases
AAGCTTTTTTGGCAATACTCTGGCATAGCAAAAGAGTCTCAGTGTGCGGTTTTGCGAAAAACACCGTTTCAGTCGAAGGT
TGAACAAGCTTAAATCTTTT

Product: histidine utilization repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MATAKFAEIKEYIRRHIEAGEWEENTRVPSENQLAEQFGCSRMTARRALTELVEAGVLERSQGLGTFVASLKSQSSMLSI
RNIADEIKGRGHGHSVQVVELAAINAIPPIAIALGLEEGTEVFYSLLVHCEQGIPLQLEERFVNPSLAPGYLRQDFSQLT
PHEYLSHVAPLTEARHTIEAIMPKEQIRNSLNIPEGEPCLQIIRRTWSRQGVVSFARLVHPGSRFRLGGHLTFK

Sequences:

>Translated_234_residues
MATAKFAEIKEYIRRHIEAGEWEENTRVPSENQLAEQFGCSRMTARRALTELVEAGVLERSQGLGTFVASLKSQSSMLSI
RNIADEIKGRGHGHSVQVVELAAINAIPPIAIALGLEEGTEVFYSLLVHCEQGIPLQLEERFVNPSLAPGYLRQDFSQLT
PHEYLSHVAPLTEARHTIEAIMPKEQIRNSLNIPEGEPCLQIIRRTWSRQGVVSFARLVHPGSRFRLGGHLTFK
>Mature_233_residues
ATAKFAEIKEYIRRHIEAGEWEENTRVPSENQLAEQFGCSRMTARRALTELVEAGVLERSQGLGTFVASLKSQSSMLSIR
NIADEIKGRGHGHSVQVVELAAINAIPPIAIALGLEEGTEVFYSLLVHCEQGIPLQLEERFVNPSLAPGYLRQDFSQLTP
HEYLSHVAPLTEARHTIEAIMPKEQIRNSLNIPEGEPCLQIIRRTWSRQGVVSFARLVHPGSRFRLGGHLTFK

Specific function: Repressor which binds to the hutP region in the histidine utilization (hut) operon. It blocks the expression of all the hut genes in the absence of inducer [H]

COG id: COG2188

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082252, Length=154, Percent_Identity=29.8701298701299, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786950, Length=215, Percent_Identity=26.9767441860465, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1790118, Length=229, Percent_Identity=23.1441048034935, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010248
- InterPro:   IPR000524
- InterPro:   IPR011663
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR; PF07702 UTRA [H]

EC number: NA

Molecular weight: Translated: 26144; Mature: 26013

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATAKFAEIKEYIRRHIEAGEWEENTRVPSENQLAEQFGCSRMTARRALTELVEAGVLER
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
SQGLGTFVASLKSQSSMLSIRNIADEIKGRGHGHSVQVVELAAINAIPPIAIALGLEEGT
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHCCCCHHHHEECCHHHH
EVFYSLLVHCEQGIPLQLEERFVNPSLAPGYLRQDFSQLTPHEYLSHVAPLTEARHTIEA
HHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IMPKEQIRNSLNIPEGEPCLQIIRRTWSRQGVVSFARLVHPGSRFRLGGHLTFK
HCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEECCC
>Mature Secondary Structure 
ATAKFAEIKEYIRRHIEAGEWEENTRVPSENQLAEQFGCSRMTARRALTELVEAGVLER
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
SQGLGTFVASLKSQSSMLSIRNIADEIKGRGHGHSVQVVELAAINAIPPIAIALGLEEGT
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHCCCCHHHHEECCHHHH
EVFYSLLVHCEQGIPLQLEERFVNPSLAPGYLRQDFSQLTPHEYLSHVAPLTEARHTIEA
HHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IMPKEQIRNSLNIPEGEPCLQIIRRTWSRQGVVSFARLVHPGSRFRLGGHLTFK
HCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]