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The map label for this gene is pleD [H]

Identifier: 119773207

GI number: 119773207

Start: 70912

End: 71829

Strand: Direct

Name: pleD [H]

Synonym: Sama_0065

Alternate gene names: 119773207

Gene position: 70912-71829 (Clockwise)

Preceding gene: 119773206

Following gene: 119773208

Centisome position: 1.65

GC content: 54.36

Gene sequence:

>918_bases
ATGATGGAGCCTTACACAGTACTCGTCGTTGACGATGTTAAAACCAACCTGATGATAATGGGCCAAAGTCTCAAGGGACT
TTACCAAATTCTCGAGGCAAGTAGCGGCGCCCAATGCCTGAAGCTCGCCCGCAGCGAGCCTCAGCCCGATCTGATTTTGT
TGGACGTGGTAATGCCTGACCTCGATGGTTATGAGGTTTGCAGAGCGCTAAAGAGCGACCCCGCAACCGCTGAGATCCCC
ATCATTTTCGTGACCGGCAAAGACTCTGATGAAGATGAGCAGTATGGGCTGGAGCTCGGTGCCGTCGATTACATCACCAA
GCCAATCAGACCGGCCATAGTGCGCGCCCGGGTCTGCGCCCACATACAGCTCAAGCAGCAGCGAGACAGGTTGCAGTCAA
TGGCGATGCACGACCAACTCACCGGGCTATATAACCGTCACTTTTTGATTGAATGCGCATCACAACGTTTGGCCAGGGTG
GCACGCCATCGTAATCCCATGACGCTGGTGTTAATGGATGTGGACCATTTCAAGCGGGTAAACGATAACTATGGCCACCA
TGTTGGCGACAAGGTACTGATTGAGGTTGCCCGCTTGCTCAGCACCAACATGCGCCGGGAGGATGTCGTCGCCCGTTTAG
GAGGGGAAGAGTTTGTGATGCTGATGGACTGTAGCCTCGATAATGCTGCTGCCAAGGTTGAGCATCTGAGACACCTGCTG
GAGGCTTTAGAGCCAGACGGCATTAAAGTTACCGGCAGCTTTGGGTTGGTAGAGGCAAGCCCCTATAACGCTGACTTCTC
CCATTTGTTGGTGTTGGCCGATGATGGGGTGTATCGCGCCAAAACACAGGGACGAAACTGTGTTGTGTGTCACCGGGCTG
AGGCGCCGGCCCTGCGGGGCAAGGTCTCGACTGATTAA

Upstream 100 bases:

>100_bases
CCAATAAAATAAAGGCGTTTACCCATAGGGTTCTGTATCTGCTGCTACACTTTTTCAGACAGGGAGTAACAGGGATGCAG
CTCAGGAGCAGACAGTTGAG

Downstream 100 bases:

>100_bases
CGGGGTGCCAAGGCATGGGAAGAATTTGTCTGTTGCTGATGATCGCTCTGCTGTTTTGTGGTTGCAGTGAGCCCAAGGAA
AAAATCATCATCGCCATCAA

Product: response regulator receiver protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MMEPYTVLVVDDVKTNLMIMGQSLKGLYQILEASSGAQCLKLARSEPQPDLILLDVVMPDLDGYEVCRALKSDPATAEIP
IIFVTGKDSDEDEQYGLELGAVDYITKPIRPAIVRARVCAHIQLKQQRDRLQSMAMHDQLTGLYNRHFLIECASQRLARV
ARHRNPMTLVLMDVDHFKRVNDNYGHHVGDKVLIEVARLLSTNMRREDVVARLGGEEFVMLMDCSLDNAAAKVEHLRHLL
EALEPDGIKVTGSFGLVEASPYNADFSHLLVLADDGVYRAKTQGRNCVVCHRAEAPALRGKVSTD

Sequences:

>Translated_305_residues
MMEPYTVLVVDDVKTNLMIMGQSLKGLYQILEASSGAQCLKLARSEPQPDLILLDVVMPDLDGYEVCRALKSDPATAEIP
IIFVTGKDSDEDEQYGLELGAVDYITKPIRPAIVRARVCAHIQLKQQRDRLQSMAMHDQLTGLYNRHFLIECASQRLARV
ARHRNPMTLVLMDVDHFKRVNDNYGHHVGDKVLIEVARLLSTNMRREDVVARLGGEEFVMLMDCSLDNAAAKVEHLRHLL
EALEPDGIKVTGSFGLVEASPYNADFSHLLVLADDGVYRAKTQGRNCVVCHRAEAPALRGKVSTD
>Mature_305_residues
MMEPYTVLVVDDVKTNLMIMGQSLKGLYQILEASSGAQCLKLARSEPQPDLILLDVVMPDLDGYEVCRALKSDPATAEIP
IIFVTGKDSDEDEQYGLELGAVDYITKPIRPAIVRARVCAHIQLKQQRDRLQSMAMHDQLTGLYNRHFLIECASQRLARV
ARHRNPMTLVLMDVDHFKRVNDNYGHHVGDKVLIEVARLLSTNMRREDVVARLGGEEFVMLMDCSLDNAAAKVEHLRHLL
EALEPDGIKVTGSFGLVEASPYNADFSHLLVLADDGVYRAKTQGRNCVVCHRAEAPALRGKVSTD

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=40.1197604790419, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=36.3636363636364, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786584, Length=196, Percent_Identity=31.6326530612245, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI145693134, Length=178, Percent_Identity=32.0224719101124, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081881, Length=171, Percent_Identity=36.2573099415205, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788085, Length=177, Percent_Identity=32.2033898305085, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI87081977, Length=181, Percent_Identity=32.5966850828729, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787802, Length=191, Percent_Identity=34.5549738219895, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788381, Length=186, Percent_Identity=32.258064516129, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786599, Length=76, Percent_Identity=44.7368421052632, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788394, Length=115, Percent_Identity=39.1304347826087, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI87081974, Length=129, Percent_Identity=31.7829457364341, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787541, Length=180, Percent_Identity=27.7777777777778, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787816, Length=156, Percent_Identity=34.6153846153846, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790860, Length=121, Percent_Identity=37.1900826446281, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788956, Length=101, Percent_Identity=36.6336633663366, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789809, Length=121, Percent_Identity=35.5371900826446, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790863, Length=119, Percent_Identity=32.7731092436975, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789149, Length=105, Percent_Identity=37.1428571428571, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693140, Length=107, Percent_Identity=36.4485981308411, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34019; Mature: 34019

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEPYTVLVVDDVKTNLMIMGQSLKGLYQILEASSGAQCLKLARSEPQPDLILLDVVMPD
CCCCEEEEEECCCCCCEEEECHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEEECCC
LDGYEVCRALKSDPATAEIPIIFVTGKDSDEDEQYGLELGAVDYITKPIRPAIVRARVCA
CCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHCCEECCHHHHHCCCHHHHHHHHHHH
HIQLKQQRDRLQSMAMHDQLTGLYNRHFLIECASQRLARVARHRNPMTLVLMDVDHFKRV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHC
NDNYGHHVGDKVLIEVARLLSTNMRREDVVARLGGEEFVMLMDCSLDNAAAKVEHLRHLL
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHH
EALEPDGIKVTGSFGLVEASPYNADFSHLLVLADDGVYRAKTQGRNCVVCHRAEAPALRG
HHCCCCCEEEEECCCEEECCCCCCCCEEEEEEECCCEEEECCCCCCEEEEECCCCCCCCC
KVSTD
CCCCC
>Mature Secondary Structure
MMEPYTVLVVDDVKTNLMIMGQSLKGLYQILEASSGAQCLKLARSEPQPDLILLDVVMPD
CCCCEEEEEECCCCCCEEEECHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEEECCC
LDGYEVCRALKSDPATAEIPIIFVTGKDSDEDEQYGLELGAVDYITKPIRPAIVRARVCA
CCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHCCEECCHHHHHCCCHHHHHHHHHHH
HIQLKQQRDRLQSMAMHDQLTGLYNRHFLIECASQRLARVARHRNPMTLVLMDVDHFKRV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHC
NDNYGHHVGDKVLIEVARLLSTNMRREDVVARLGGEEFVMLMDCSLDNAAAKVEHLRHLL
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHH
EALEPDGIKVTGSFGLVEASPYNADFSHLLVLADDGVYRAKTQGRNCVVCHRAEAPALRG
HHCCCCCEEEEECCCEEECCCCCCCCEEEEEEECCCEEEECCCCCCEEEEECCCCCCCCC
KVSTD
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA