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The map label for this gene is yafC [C]

Identifier: 119773147

GI number: 119773147

Start: 3686

End: 4558

Strand: Direct

Name: yafC [C]

Synonym: Sama_0005

Alternate gene names: 119773147

Gene position: 3686-4558 (Clockwise)

Preceding gene: 119773144

Following gene: 119773148

Centisome position: 0.09

GC content: 57.73

Gene sequence:

>873_bases
ATGCGCTGGGAAGGGATTTGTGAATTTGTTGCCGTAGCCGAAGCGCAAAGCTTTACCGCTGCAGCTGGCCGGCTTGGGAT
CAGTACGGCGCAGGTGAGCCGGCAGGTGCGGGAGCTCGAAGAGCGCCTCGGCAGCAAACTTCTGTATCGCACTACACGTA
AGGTGTCGCTGACCGAAGACGGTCAGCTGTTTTACCGCCATTGCCGCCAGGTGCTGGATGGCTTGGATGAAGCCGAGCGG
GCAGTGTCGTCACTGAAGAGCGAACCGCGTGGGCTTATCCGTATGACGGCGCCTGTTACCTACGGTGAGCAGTACGTGAT
GCCGATAGTGCTCGCGTACATGCAGCAATTTCCCCAGGTGGAAGTGCAGTGTGAGCTCACTAATCAACAGCTCGATTTGG
TGCAGGGCGGTTTTGATTTGGCGATTCGCCTCGGTGTGCTGCCGGATTCTTCCATGATGGCGCGAAAGCTGGCCGAGCGG
GTGCAGTATCTGGTGGCATCACCGGATTATGTCAGCCGCCACGGCGCGCCCCATTCGCTTTCAGAGCTCAGTCGTCACCA
GTGTTTGCTGGGGAGCCTGCCATTTTGGCGCTTTCAGGAAGAGGGCAAGCTTCGCAGCCTGAAGGTGAAGGGGAGGCTCA
GTTGCAGCAGCGGCAATACCTTGCTGGCTGCGGCACTGGCGGGCATGGGGATAGCTCAATTGCCCGGCTATTATGTAGAT
GAAGCCATTCGTGACGGCCGCTTGCTGGTGCTGCTCAAACCATTTCAGGAGCCCAAAGAAGGCATTTGGGGCCTGTATCC
CCATAATCGGCAGCTGTCACCCAAGATTGGCCATCTGATGAATATGCTGGCAGACAAGTTGCCCAAAGCCTGA

Upstream 100 bases:

>100_bases
TATGGCAAAGACGCTTTGTGTGTGAATGTGATTGTATTTATTTACTGTGAATTGATAATTGGCTCTATTGGTAAATAACT
TTTACTGGATAGAAATAATT

Downstream 100 bases:

>100_bases
TTAGAGGTGGTGCGCCAATCTAGCTCGTTTCAACTTGTTGAAAATGGCCGAATAACCTACGGTGATTCTGGCAAGACGTT
TTGGCGGAACACTTTGCTAA

Product: LysR, substrate-binding

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRWEGICEFVAVAEAQSFTAAAGRLGISTAQVSRQVRELEERLGSKLLYRTTRKVSLTEDGQLFYRHCRQVLDGLDEAER
AVSSLKSEPRGLIRMTAPVTYGEQYVMPIVLAYMQQFPQVEVQCELTNQQLDLVQGGFDLAIRLGVLPDSSMMARKLAER
VQYLVASPDYVSRHGAPHSLSELSRHQCLLGSLPFWRFQEEGKLRSLKVKGRLSCSSGNTLLAAALAGMGIAQLPGYYVD
EAIRDGRLLVLLKPFQEPKEGIWGLYPHNRQLSPKIGHLMNMLADKLPKA

Sequences:

>Translated_290_residues
MRWEGICEFVAVAEAQSFTAAAGRLGISTAQVSRQVRELEERLGSKLLYRTTRKVSLTEDGQLFYRHCRQVLDGLDEAER
AVSSLKSEPRGLIRMTAPVTYGEQYVMPIVLAYMQQFPQVEVQCELTNQQLDLVQGGFDLAIRLGVLPDSSMMARKLAER
VQYLVASPDYVSRHGAPHSLSELSRHQCLLGSLPFWRFQEEGKLRSLKVKGRLSCSSGNTLLAAALAGMGIAQLPGYYVD
EAIRDGRLLVLLKPFQEPKEGIWGLYPHNRQLSPKIGHLMNMLADKLPKA
>Mature_290_residues
MRWEGICEFVAVAEAQSFTAAAGRLGISTAQVSRQVRELEERLGSKLLYRTTRKVSLTEDGQLFYRHCRQVLDGLDEAER
AVSSLKSEPRGLIRMTAPVTYGEQYVMPIVLAYMQQFPQVEVQCELTNQQLDLVQGGFDLAIRLGVLPDSSMMARKLAER
VQYLVASPDYVSRHGAPHSLSELSRHQCLLGSLPFWRFQEEGKLRSLKVKGRLSCSSGNTLLAAALAGMGIAQLPGYYVD
EAIRDGRLLVLLKPFQEPKEGIWGLYPHNRQLSPKIGHLMNMLADKLPKA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=35.1535836177474, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI145693193, Length=282, Percent_Identity=32.2695035460993, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1787589, Length=297, Percent_Identity=32.6599326599327, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI87081978, Length=273, Percent_Identity=33.6996336996337, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1789440, Length=263, Percent_Identity=32.6996197718631, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1789639, Length=288, Percent_Identity=30.2083333333333, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787128, Length=279, Percent_Identity=29.0322580645161, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1790011, Length=271, Percent_Identity=25.830258302583, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788706, Length=188, Percent_Identity=27.6595744680851, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1789173, Length=251, Percent_Identity=25.8964143426295, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787879, Length=299, Percent_Identity=25.752508361204, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788297, Length=241, Percent_Identity=27.8008298755187, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI157672245, Length=125, Percent_Identity=31.2, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1786448, Length=241, Percent_Identity=23.6514522821577, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790262, Length=261, Percent_Identity=28.3524904214559, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082132, Length=296, Percent_Identity=24.6621621621622, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786713, Length=272, Percent_Identity=27.2058823529412, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788481, Length=289, Percent_Identity=24.2214532871972, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787601, Length=137, Percent_Identity=29.1970802919708, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32417; Mature: 32417

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWEGICEFVAVAEAQSFTAAAGRLGISTAQVSRQVRELEERLGSKLLYRTTRKVSLTED
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCC
GQLFYRHCRQVLDGLDEAERAVSSLKSEPRGLIRMTAPVTYGEQYVMPIVLAYMQQFPQV
HHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCE
EVQCELTNQQLDLVQGGFDLAIRLGVLPDSSMMARKLAERVQYLVASPDYVSRHGAPHSL
EEEEEECCHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCH
SELSRHQCLLGSLPFWRFQEEGKLRSLKVKGRLSCSSGNTLLAAALAGMGIAQLPGYYVD
HHHHHHHHHHCCCCCCEECCCCCEEEEEEEEEEEECCCCHHHHHHHHCCCHHHCCCHHHH
EAIRDGRLLVLLKPFQEPKEGIWGLYPHNRQLSPKIGHLMNMLADKLPKA
HHHCCCCEEEEECCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRWEGICEFVAVAEAQSFTAAAGRLGISTAQVSRQVRELEERLGSKLLYRTTRKVSLTED
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCC
GQLFYRHCRQVLDGLDEAERAVSSLKSEPRGLIRMTAPVTYGEQYVMPIVLAYMQQFPQV
HHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCE
EVQCELTNQQLDLVQGGFDLAIRLGVLPDSSMMARKLAERVQYLVASPDYVSRHGAPHSL
EEEEEECCHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCH
SELSRHQCLLGSLPFWRFQEEGKLRSLKVKGRLSCSSGNTLLAAALAGMGIAQLPGYYVD
HHHHHHHHHHCCCCCCEECCCCCEEEEEEEEEEEECCCCHHHHHHHHCCCHHHCCCHHHH
EAIRDGRLLVLLKPFQEPKEGIWGLYPHNRQLSPKIGHLMNMLADKLPKA
HHHCCCCEEEEECCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]