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The map label for this gene is folP2 [H]

Identifier: 119718375

GI number: 119718375

Start: 4390905

End: 4391768

Strand: Reverse

Name: folP2 [H]

Synonym: Noca_4156

Alternate gene names: 119718375

Gene position: 4391768-4390905 (Counterclockwise)

Preceding gene: 119718376

Following gene: 119718374

Centisome position: 88.08

GC content: 72.92

Gene sequence:

>864_bases
ATGACCCTCAAGCTCGGCCGCCACACCTTCCCCGACACCGCCACGTTGATGATGGCGATCGTCAACCGCACCCCGGACTC
GTTCTACGACAAGGGCGCGACCTGGGCGGAGGACAAGGCCTTCGAGCGGGTCGCGCTGGTCGCCGGCGAGGGCGCCGAGA
TCGTCGACATCGGCGGCATCAAGGCCGCGCCCGGCGTCGAGATCTCCGAGGCCGAGGAGAAGGCCCGGGTCGTCGACTTC
GTGGCCCGGGTGCGCGCGTCGTACCCGGACCTCGTGATCTCGGTCGACACCTGGCGCGCCGAGGTCGGCAGCGCCGTCTG
CGCGGCCGGCGCCGACCTGATCAACGACGCCTGGGGCGGCGCGGACCCCGAGCTCGCCGACGCGGCCGCCGAGGCGGGCG
CCGCGATCGTGTGCACCCACACCGGCGGGGTCACCCCGCGGACCCGTCCCTACCGGATGGAGTACGACGACGTGGTCGCC
GCCGCGATCGCCGACACCGTCGCGTACGCCGAGCGCGCGATCGCCGCCGGCGTCGACCCCGCCTCGGTCGTCATCGACCC
GGCCCACGACTTCGGCAAGAACACCTTCCACTCCCTCGAGGTCACCCGCCGGCTCGGTGAGATGGTCGCGACCGGGTACC
CCGTCCTGGTGTCGCTCTCCAACAAGGACTTCGTCGGCGAGAGCCTCGACCTGCCGGTCGGGGAGCGGCTGACCGGCACC
CTGGCCGCCACCGCCGTCTGTGCCCTCGCCGGCGCCCGGATCTACCGCGTGCACGAGGTCGTCGAGACCCGCCAGACCGT
CGACATGGTCGACACGATCGCCGGTCGCCGCCCGCCCCGCCTCGCCATCCGGGGGCTGCAATGA

Upstream 100 bases:

>100_bases
GCGGGATGGCCACCCAGATCCTCGCGGCCGCCCTGGCCCGGGTCGGCACCCCCACCGCGGCCGGGCCGGCCGACGAGCGA
CCGCCGGCGGTGGGCGTGCG

Downstream 100 bases:

>100_bases
GAGTGGTCCTGGTCCCCGGCGTGCTCGCGCTGCTGCCGGAGTACGCCGGCCTCGAGGACCCGGTCCCCGAGCTGCGGGCC
GCCTGCGTGGCGGCCGTGCG

Product: dihydropteroate synthase

Products: diphosphate; dihydropteroate

Alternate protein names: DHPS 2; Dihydropteroate pyrophosphorylase 2 [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MTLKLGRHTFPDTATLMMAIVNRTPDSFYDKGATWAEDKAFERVALVAGEGAEIVDIGGIKAAPGVEISEAEEKARVVDF
VARVRASYPDLVISVDTWRAEVGSAVCAAGADLINDAWGGADPELADAAAEAGAAIVCTHTGGVTPRTRPYRMEYDDVVA
AAIADTVAYAERAIAAGVDPASVVIDPAHDFGKNTFHSLEVTRRLGEMVATGYPVLVSLSNKDFVGESLDLPVGERLTGT
LAATAVCALAGARIYRVHEVVETRQTVDMVDTIAGRRPPRLAIRGLQ

Sequences:

>Translated_287_residues
MTLKLGRHTFPDTATLMMAIVNRTPDSFYDKGATWAEDKAFERVALVAGEGAEIVDIGGIKAAPGVEISEAEEKARVVDF
VARVRASYPDLVISVDTWRAEVGSAVCAAGADLINDAWGGADPELADAAAEAGAAIVCTHTGGVTPRTRPYRMEYDDVVA
AAIADTVAYAERAIAAGVDPASVVIDPAHDFGKNTFHSLEVTRRLGEMVATGYPVLVSLSNKDFVGESLDLPVGERLTGT
LAATAVCALAGARIYRVHEVVETRQTVDMVDTIAGRRPPRLAIRGLQ
>Mature_286_residues
TLKLGRHTFPDTATLMMAIVNRTPDSFYDKGATWAEDKAFERVALVAGEGAEIVDIGGIKAAPGVEISEAEEKARVVDFV
ARVRASYPDLVISVDTWRAEVGSAVCAAGADLINDAWGGADPELADAAAEAGAAIVCTHTGGVTPRTRPYRMEYDDVVAA
AIADTVAYAERAIAAGVDPASVVIDPAHDFGKNTFHSLEVTRRLGEMVATGYPVLVSLSNKDFVGESLDLPVGERLTGTL
AATAVCALAGARIYRVHEVVETRQTVDMVDTIAGRRPPRLAIRGLQ

Specific function: Has very low affinity for the DHPS substrate 6- hydroxymethyl-7,8-dihydropterin-pyrophosphate, but can bind the inhibitor dapsone. Seems to lack dihydropteroate synthase activity, and does probably not function in folate metabolism [H]

COG id: COG0294

COG function: function code H; Dihydropteroate synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082224, Length=266, Percent_Identity=34.9624060150376, Blast_Score=146, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006390
- InterPro:   IPR011005
- InterPro:   IPR000489 [H]

Pfam domain/function: PF00809 Pterin_bind [H]

EC number: 2.5.1.15

Molecular weight: Translated: 30337; Mature: 30205

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50972 PTERIN_BINDING ; PS00792 DHPS_1 ; PS00793 DHPS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKLGRHTFPDTATLMMAIVNRTPDSFYDKGATWAEDKAFERVALVAGEGAEIVDIGGI
CEEECCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHEEEEEECCCCCEEEECCC
KAAPGVEISEAEEKARVVDFVARVRASYPDLVISVDTWRAEVGSAVCAAGADLINDAWGG
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCHHHHHHCCCC
ADPELADAAAEAGAAIVCTHTGGVTPRTRPYRMEYDDVVAAAIADTVAYAERAIAAGVDP
CCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
ASVVIDPAHDFGKNTFHSLEVTRRLGEMVATGYPVLVSLSNKDFVGESLDLPVGERLTGT
HHEEEECCHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHH
LAATAVCALAGARIYRVHEVVETRQTVDMVDTIAGRRPPRLAIRGLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TLKLGRHTFPDTATLMMAIVNRTPDSFYDKGATWAEDKAFERVALVAGEGAEIVDIGGI
EEECCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHEEEEEECCCCCEEEECCC
KAAPGVEISEAEEKARVVDFVARVRASYPDLVISVDTWRAEVGSAVCAAGADLINDAWGG
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCHHHHHHCCCC
ADPELADAAAEAGAAIVCTHTGGVTPRTRPYRMEYDDVVAAAIADTVAYAERAIAAGVDP
CCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
ASVVIDPAHDFGKNTFHSLEVTRRLGEMVATGYPVLVSLSNKDFVGESLDLPVGERLTGT
HHEEEECCHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHH
LAATAVCALAGARIYRVHEVVETRQTVDMVDTIAGRRPPRLAIRGLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0015 {4-aminobenzoate}} [C]

Substrates: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate; 4-aminobenzoate

Specific reaction: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate

General reaction: Aryl group transfer [C]

Inhibitor: Sulfaguanidine; Sulfanilamide; Sulfathiazole; Sulfonamides [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]