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The map label for this gene is truA [H]

Identifier: 119718091

GI number: 119718091

Start: 4089775

End: 4090581

Strand: Reverse

Name: truA [H]

Synonym: Noca_3871

Alternate gene names: 119718091

Gene position: 4090581-4089775 (Counterclockwise)

Preceding gene: 119718092

Following gene: 119718090

Centisome position: 82.04

GC content: 75.34

Gene sequence:

>807_bases
GTGCGGATCCGGCTCGACCTCGCCTACGACGGCACCGACTTCCACGGCTGGGCGAGCCAGCCGGGGCTGCGGACCGTGCA
GGGCGAGCTGAGCGACGCCCTCGCGACGGTGCTGCGGGTCCCGGAGGTCGCCGTGGTCTGCGCCGGCCGCACCGACGCCG
GCGTCCACGCGCGCGGCCAGGTGGTGCACGCCGACCTGGACGGCCTCGAGCCGGCCACCGTCGAGGGGCTGGTACGGCGG
CTCAACGGGCGACTGCCCGCCGACGTACGGGTGCGCCGGGCGGTCGAGGCGCCCGCGGGGTTCGACGCCCGGTTCGGGGC
ACTGTGGCGGCGCTACGCCTACCGGATCGCCGACACCCCGGCCGTAGTGGACCCGCTGACCCGCTCCCACGTCCTGGCCT
GGCCACGAGCCCTGGACCTCGACGCGATGAACGCGGCGTCCGCGTTCCTGGTCGGCCGGCACGACTTCGCGTCCTTCTGC
AAGCAGCGGGAGGGCGCGACCACGATCCGGACCCTGCTTGAGCTGTCCTGGGCGCGGGACCCGGCCGGCGTGCTGGTCGG
GACGGTCCGCGCCGACGCGTTCTGCCACAGCATGGTCCGGTCCCTGGTCGGCTGCCTGATCGCCGTCGGGGAGGCGCGCA
AGCCGGTCGCCTGGGCCGAGGACATCCTGCGGGCGGAGGCGCGTGATCCCGCGGTGGCGGTCGTGCACGCCCACGGCCTC
ACGCTGGAGGAGGTCGCCTACCCGGCCGACGACGAGCTCGCCGCCCGGGCCGAGCAGACACGAGCGAAGAGGGTCGCCGA
TGAGTGA

Upstream 100 bases:

>100_bases
TCCCTGACGAACGCGCACGCGACCCGCCGGCGGGGGTGTGCGACAGGTCAGGGACGTCCCCGTCCCGGAGCAGCGTCGAG
TACCGCAATAGGGTTGGCCC

Downstream 100 bases:

>100_bases
GGACCACTACTTCAGCGCCGACCCGTCGGTGCCGTTCGAGCGCGAGCCGTTCACCTGCGAGGTGTGGGGCCGGGAGCTGA
CGCTGGTCAGCGGCTCCGGT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MRIRLDLAYDGTDFHGWASQPGLRTVQGELSDALATVLRVPEVAVVCAGRTDAGVHARGQVVHADLDGLEPATVEGLVRR
LNGRLPADVRVRRAVEAPAGFDARFGALWRRYAYRIADTPAVVDPLTRSHVLAWPRALDLDAMNAASAFLVGRHDFASFC
KQREGATTIRTLLELSWARDPAGVLVGTVRADAFCHSMVRSLVGCLIAVGEARKPVAWAEDILRAEARDPAVAVVHAHGL
TLEEVAYPADDELAARAEQTRAKRVADE

Sequences:

>Translated_268_residues
MRIRLDLAYDGTDFHGWASQPGLRTVQGELSDALATVLRVPEVAVVCAGRTDAGVHARGQVVHADLDGLEPATVEGLVRR
LNGRLPADVRVRRAVEAPAGFDARFGALWRRYAYRIADTPAVVDPLTRSHVLAWPRALDLDAMNAASAFLVGRHDFASFC
KQREGATTIRTLLELSWARDPAGVLVGTVRADAFCHSMVRSLVGCLIAVGEARKPVAWAEDILRAEARDPAVAVVHAHGL
TLEEVAYPADDELAARAEQTRAKRVADE
>Mature_268_residues
MRIRLDLAYDGTDFHGWASQPGLRTVQGELSDALATVLRVPEVAVVCAGRTDAGVHARGQVVHADLDGLEPATVEGLVRR
LNGRLPADVRVRRAVEAPAGFDARFGALWRRYAYRIADTPAVVDPLTRSHVLAWPRALDLDAMNAASAFLVGRHDFASFC
KQREGATTIRTLLELSWARDPAGVLVGTVRADAFCHSMVRSLVGCLIAVGEARKPVAWAEDILRAEARDPAVAVVHAHGL
TLEEVAYPADDELAARAEQTRAKRVADE

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=277, Percent_Identity=33.5740072202166, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI199559785, Length=264, Percent_Identity=28.030303030303, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788657, Length=254, Percent_Identity=38.5826771653543, Blast_Score=148, Evalue=4e-37,
Organism=Drosophila melanogaster, GI116007752, Length=306, Percent_Identity=26.797385620915, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 29024; Mature: 29024

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIRLDLAYDGTDFHGWASQPGLRTVQGELSDALATVLRVPEVAVVCAGRTDAGVHARGQ
CEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
VVHADLDGLEPATVEGLVRRLNGRLPADVRVRRAVEAPAGFDARFGALWRRYAYRIADTP
EEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
AVVDPLTRSHVLAWPRALDLDAMNAASAFLVGRHDFASFCKQREGATTIRTLLELSWARD
HHHCCHHHHHHHHCCCCCCCHHHHHHHHHEECCHHHHHHHHHCCCHHHHHHHHHHHHCCC
PAGVLVGTVRADAFCHSMVRSLVGCLIAVGEARKPVAWAEDILRAEARDPAVAVVHAHGL
CCHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEECCC
TLEEVAYPADDELAARAEQTRAKRVADE
CHHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRIRLDLAYDGTDFHGWASQPGLRTVQGELSDALATVLRVPEVAVVCAGRTDAGVHARGQ
CEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
VVHADLDGLEPATVEGLVRRLNGRLPADVRVRRAVEAPAGFDARFGALWRRYAYRIADTP
EEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
AVVDPLTRSHVLAWPRALDLDAMNAASAFLVGRHDFASFCKQREGATTIRTLLELSWARD
HHHCCHHHHHHHHCCCCCCCHHHHHHHHHEECCHHHHHHHHHCCCHHHHHHHHHHHHCCC
PAGVLVGTVRADAFCHSMVRSLVGCLIAVGEARKPVAWAEDILRAEARDPAVAVVHAHGL
CCHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEECCC
TLEEVAYPADDELAARAEQTRAKRVADE
CHHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA