| Definition | Nocardioides sp. JS614 chromosome, complete genome. |
|---|---|
| Accession | NC_008699 |
| Length | 4,985,871 |
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The map label for this gene is kpnIM [H]
Identifier: 119717929
GI number: 119717929
Start: 3935260
End: 3936438
Strand: Reverse
Name: kpnIM [H]
Synonym: Noca_3707
Alternate gene names: 119717929
Gene position: 3936438-3935260 (Counterclockwise)
Preceding gene: 119717933
Following gene: 119717926
Centisome position: 78.95
GC content: 71.59
Gene sequence:
>1179_bases GTGGGCCAGCCGTGGAACGTCTTCGTCGAGGGCGACAACCTCGACGTGCTACCCCGGGTGCCGGAGCTGGTCGGCCGGCC GGTCGACCTGGTCTACATCGACCCGCCGTACAACACCGGCAACGACTTCGCCTACCACGACGACTTCCGCTGCCACGCCG CGTGGGTGGAGATGATGCGCCCGCGACTGACGGCCGCGCGCGAGGTGCTCGCCGAGACCGGCGCGATCTTCGTCAGCATC GACGACCACGAGGTCGCCCATCTCCGGTTGCTGATGGACGAGGTGTACGGCGAGCAGAACCTGCTCGCCCAGGTCGTGGT GAACCTCAACCCGAAGGGGCGCCAGCTCGGCAGGGGTTTCGCCACCAGCCACGAGTACCTGCTCGTGTACGCCCGCGACG CCCGTCGTACGGTCCTCGACGCGGCGACCCACGAGACCGTCGACCCGAGCGACTTCCCGTTGGAGAGCGCGGATGGCCGG CGGTACCGGCAGCTGCCGCTTCGCAACACCAACAAGAAGTTCAACCCGGTCACCGCGCGTACCCTGCACTTCACGGTGTG GGGCGACCCCCAGACGGGCCGGGTCGCGACCGTGCCGTTCGAGGGGGCCGTCGAGATCGGGCCGGTGTTCGGCGACGGTA CGCCGGCGGTGTGGCGGTGGAGCCGGCCGCTGATCGACCAGCGGCCCGACGACCTCGTGTGCCGCCGGGTGAAGGGGCGC CTGGGGGAGCGGGTCGACGTGTTCCAGAAGGACTGGCTGCACCCCGTCGCCGCCGGTGGCGTCCCGGGCGTGCGGCGCAA GAAGCTGCGCACGATCTGGCTCGCCGAGGAGGTCGGGTCCACCGACACGGCGGTCGCCGAGCTCAAGGAGGTCGTCGGGC ACGTCTTCGAGTCGCCGAAGCCGACCGGCCTGCTCCGCCGGATCCTCGGCACCATGCCCGGCGACGCGGTCGTGCTCGAC TTCTTCGCAGGCAGCGGCACCACCGGTCACGCCGTCGCGCTGCAGAACGCCGCCGACGGCGGCACCCGCCGTTGCGTCAG CATCAACTCCGCGGAGCCGACCCGCGAGGGCTCCAACGCCCGCAACGCGGGACTGCGCACCGTCGCCGACATCACCCGCG CCCGGCTGCGGGCGGTCGCTGAGCGGGTCGGCGGTGGGTTCGAGGAGGTCCGGCTCTAG
Upstream 100 bases:
>100_bases CCGAGAGCATGCGGAACACGCGGCGATTCTCGGGCCTCCGCGGGCCTGCCGGGGGCGTTTCGCCCATCTTCGGGCGCGCC TGCGCCACTACTGTCGGGCC
Downstream 100 bases:
>100_bases ACCCCGGCCGCCTTCTCCAACGCCCGGACCTCGTCGTCGAGGTGGGTGAGCAGCCGCTGCAGGTGGGGGACGGTCCGGCG GCAGCCGATCAGCCCGAAGG
Product: adenine-specific DNA-methyltransferase
Products: NA
Alternate protein names: M.KpnI; Adenine-specific methyltransferase KpnI [H]
Number of amino acids: Translated: 392; Mature: 391
Protein sequence:
>392_residues MGQPWNVFVEGDNLDVLPRVPELVGRPVDLVYIDPPYNTGNDFAYHDDFRCHAAWVEMMRPRLTAAREVLAETGAIFVSI DDHEVAHLRLLMDEVYGEQNLLAQVVVNLNPKGRQLGRGFATSHEYLLVYARDARRTVLDAATHETVDPSDFPLESADGR RYRQLPLRNTNKKFNPVTARTLHFTVWGDPQTGRVATVPFEGAVEIGPVFGDGTPAVWRWSRPLIDQRPDDLVCRRVKGR LGERVDVFQKDWLHPVAAGGVPGVRRKKLRTIWLAEEVGSTDTAVAELKEVVGHVFESPKPTGLLRRILGTMPGDAVVLD FFAGSGTTGHAVALQNAADGGTRRCVSINSAEPTREGSNARNAGLRTVADITRARLRAVAERVGGGFEEVRL
Sequences:
>Translated_392_residues MGQPWNVFVEGDNLDVLPRVPELVGRPVDLVYIDPPYNTGNDFAYHDDFRCHAAWVEMMRPRLTAAREVLAETGAIFVSI DDHEVAHLRLLMDEVYGEQNLLAQVVVNLNPKGRQLGRGFATSHEYLLVYARDARRTVLDAATHETVDPSDFPLESADGR RYRQLPLRNTNKKFNPVTARTLHFTVWGDPQTGRVATVPFEGAVEIGPVFGDGTPAVWRWSRPLIDQRPDDLVCRRVKGR LGERVDVFQKDWLHPVAAGGVPGVRRKKLRTIWLAEEVGSTDTAVAELKEVVGHVFESPKPTGLLRRILGTMPGDAVVLD FFAGSGTTGHAVALQNAADGGTRRCVSINSAEPTREGSNARNAGLRTVADITRARLRAVAERVGGGFEEVRL >Mature_391_residues GQPWNVFVEGDNLDVLPRVPELVGRPVDLVYIDPPYNTGNDFAYHDDFRCHAAWVEMMRPRLTAAREVLAETGAIFVSID DHEVAHLRLLMDEVYGEQNLLAQVVVNLNPKGRQLGRGFATSHEYLLVYARDARRTVLDAATHETVDPSDFPLESADGRR YRQLPLRNTNKKFNPVTARTLHFTVWGDPQTGRVATVPFEGAVEIGPVFGDGTPAVWRWSRPLIDQRPDDLVCRRVKGRL GERVDVFQKDWLHPVAAGGVPGVRRKKLRTIWLAEEVGSTDTAVAELKEVVGHVFESPKPTGLLRRILGTMPGDAVVLDF FAGSGTTGHAVALQNAADGGTRRCVSINSAEPTREGSNARNAGLRTVADITRARLRAVAERVGGGFEEVRL
Specific function: This methylase recognizes the double-stranded sequence GGTACC, causes specific methylation on A-4 on both strands, and protects the DNA from cleavage by the KpnI endonuclease [H]
COG id: COG2189
COG function: function code L; Adenine specific DNA methylase Mod
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002295 - InterPro: IPR002941 - InterPro: IPR002052 [H]
Pfam domain/function: PF01555 N6_N4_Mtase [H]
EC number: =2.1.1.72 [H]
Molecular weight: Translated: 43307; Mature: 43175
Theoretical pI: Translated: 7.07; Mature: 7.07
Prosite motif: PS00092 N6_MTASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 1.3 %Met (Translated Protein) 2.0 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 1.0 %Met (Mature Protein) 1.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MGQPWNVFVEGDNLDVLPRVPELVGRPVDLVYIDPPYNTGNDFAYHDDFRCHAAWVEMMR CCCCEEEEEECCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCEECCCCHHHHHHHHHHH PRLTAAREVLAETGAIFVSIDDHEVAHLRLLMDEVYGEQNLLAQVVVNLNPKGRQLGRGF HHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHCCCC ATSHEYLLVYARDARRTVLDAATHETVDPSDFPLESADGRRYRQLPLRNTNKKFNPVTAR CCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEE TLHFTVWGDPQTGRVATVPFEGAVEIGPVFGDGTPAVWRWSRPLIDQRPDDLVCRRVKGR EEEEEEECCCCCCCEEEECCCCCEEECCEECCCCCCEEECCCCCCCCCCHHHHHHHHHHH LGERVDVFQKDWLHPVAAGGVPGVRRKKLRTIWLAEEVGSTDTAVAELKEVVGHVFESPK CCCHHHHHHHHCCCHHHCCCCCCHHHHHHHEEEEHHHHCCCHHHHHHHHHHHHHHHCCCC PTGLLRRILGTMPGDAVVLDFFAGSGTTGHAVALQNAADGGTRRCVSINSAEPTREGSNA CHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCC RNAGLRTVADITRARLRAVAERVGGGFEEVRL CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCC >Mature Secondary Structure GQPWNVFVEGDNLDVLPRVPELVGRPVDLVYIDPPYNTGNDFAYHDDFRCHAAWVEMMR CCCEEEEEECCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCEECCCCHHHHHHHHHHH PRLTAAREVLAETGAIFVSIDDHEVAHLRLLMDEVYGEQNLLAQVVVNLNPKGRQLGRGF HHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHCCCC ATSHEYLLVYARDARRTVLDAATHETVDPSDFPLESADGRRYRQLPLRNTNKKFNPVTAR CCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEE TLHFTVWGDPQTGRVATVPFEGAVEIGPVFGDGTPAVWRWSRPLIDQRPDDLVCRRVKGR EEEEEEECCCCCCCEEEECCCCCEEECCEECCCCCCEEECCCCCCCCCCHHHHHHHHHHH LGERVDVFQKDWLHPVAAGGVPGVRRKKLRTIWLAEEVGSTDTAVAELKEVVGHVFESPK CCCHHHHHHHHCCCHHHCCCCCCHHHHHHHEEEEHHHHCCCHHHHHHHHHHHHHHHCCCC PTGLLRRILGTMPGDAVVLDFFAGSGTTGHAVALQNAADGGTRRCVSINSAEPTREGSNA CHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCC RNAGLRTVADITRARLRAVAERVGGGFEEVRL CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 1754388 [H]