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The map label for this gene is iscS [H]

Identifier: 119717668

GI number: 119717668

Start: 3653349

End: 3654548

Strand: Reverse

Name: iscS [H]

Synonym: Noca_3446

Alternate gene names: 119717668

Gene position: 3654548-3653349 (Counterclockwise)

Preceding gene: 119717669

Following gene: 119717667

Centisome position: 73.3

GC content: 74.75

Gene sequence:

>1200_bases
ATGACTGCAGCTGCCCCGAGCCGGACCGTCTACCTCGACCACGCCGCGACCACCCCGATGGTCCCCGCGGCGGTGGAGGC
GATGACCGCGCACCTGCGCGAGGTCGGCAATCCGAGTTCGCTGCACGCGTCCGGCCGCCGAGCTCGCCGGGTCGTCGAGG
AGTCGCGGGAGACGATCGCGCAGGCGCTGAACTGTCGGCCGGGAGAGGTCGTGTTCACCTCCGGCGGCACCGAGGCCGAC
AACCTGGCGGTCAAGGGGATCTTCTGGTCCCGCCGCGACGAGGACGCCCGCCGCACCCGTGTGCTGACCACCGCGGTCGA
GCACCACGCGGTGCTCGACGCCGTGCACTGGCTGGGCCACGCCGAGGGCGCCGAGGTCGAGCTGCTGCCGGTCGACGACC
GGGCCCGGCTCGACGTCGACGCACTGCGCTCCGCCATCGAGCGTGACCCCGCGTCGGTCGCCGTCGTCTCGGTGATGTGG
GCCAACAACGAGGTCGGCACCCTCCAGCCGATCCACGACGTCGTGGCGCTCGCGGCCGAGCACGGCATCCCGGTGCACAC
CGACGCCGTGCAGGCCGTCGGGCACGTGCCGGTCGACTTCGCCGCATCGGGCATCGACGCACTGACCCTCACCGGCCACA
AGCTCGGCGGGCCGTACGGCGTGGGCGCGCTCGTGGTGCGCCGGGAGGTGCCGGTGCGGCCGCTGGTCCACGGCGGCGGC
CAGGAGCGCGACATCCGCAGTGGCACCCTCGACACCCCGGCGATCGCCGGGTTCGCCGCCGCCGTCGAGCTGGCCGTCAA
GCACCAGGCCGACCACGCGACGCACATCGGCGCGCTGCGTGACGAGCTGGTCCGCCGGGTGATCGAGGTGGTGCCCGACG
CGCACCTGCACGGCGCGGCGCTGGACCTCGGGGGGACCGAGCGGCTCCCGGGCAACGCCCACCTCGGCTTCCCCGGCTGC
GAGGGCGACTCGCTGCTGATGCTGCTCGACGCGCGCGGCATCGAGTGCTCCACGGGATCGGCCTGCTCCGCCGGCGTGCC
CCAGCCCTCCCACGTGCTCCTCGCGATGGGCTGCGACGCCGACCAGGCCCGCCACTCGCTGCGCTTCTCGCTCGGTCACA
CGACCACCGCGGCCGACGTCGACGCCGTCGCCGAGGCGATCGGGCCGGTCGTCGAGCGGGCCCGGGCCGCCCGGAGGTGA

Upstream 100 bases:

>100_bases
GCGGTCCTCGCCCGCACGTGACGCTCCGGCCCCGGCGCACGTCCGAGGGGGCCGGTCCGTTGGTGGCCGAGTCCGGCGGC
GCCGTACGCTGGTCGGTGCG

Downstream 100 bases:

>100_bases
TCGCGGGATGAGGGTCCTCGCCGCCATGTCCGGCGGCGTCGACTCCGCGGTGGCCGCTGCGCGCGCGGCCGAGGCGGGGC
ACGACGTCACCGGCATCCAC

Product: class V aminotransferase

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTAAAPSRTVYLDHAATTPMVPAAVEAMTAHLREVGNPSSLHASGRRARRVVEESRETIAQALNCRPGEVVFTSGGTEAD
NLAVKGIFWSRRDEDARRTRVLTTAVEHHAVLDAVHWLGHAEGAEVELLPVDDRARLDVDALRSAIERDPASVAVVSVMW
ANNEVGTLQPIHDVVALAAEHGIPVHTDAVQAVGHVPVDFAASGIDALTLTGHKLGGPYGVGALVVRREVPVRPLVHGGG
QERDIRSGTLDTPAIAGFAAAVELAVKHQADHATHIGALRDELVRRVIEVVPDAHLHGAALDLGGTERLPGNAHLGFPGC
EGDSLLMLLDARGIECSTGSACSAGVPQPSHVLLAMGCDADQARHSLRFSLGHTTTAADVDAVAEAIGPVVERARAARR

Sequences:

>Translated_399_residues
MTAAAPSRTVYLDHAATTPMVPAAVEAMTAHLREVGNPSSLHASGRRARRVVEESRETIAQALNCRPGEVVFTSGGTEAD
NLAVKGIFWSRRDEDARRTRVLTTAVEHHAVLDAVHWLGHAEGAEVELLPVDDRARLDVDALRSAIERDPASVAVVSVMW
ANNEVGTLQPIHDVVALAAEHGIPVHTDAVQAVGHVPVDFAASGIDALTLTGHKLGGPYGVGALVVRREVPVRPLVHGGG
QERDIRSGTLDTPAIAGFAAAVELAVKHQADHATHIGALRDELVRRVIEVVPDAHLHGAALDLGGTERLPGNAHLGFPGC
EGDSLLMLLDARGIECSTGSACSAGVPQPSHVLLAMGCDADQARHSLRFSLGHTTTAADVDAVAEAIGPVVERARAARR
>Mature_398_residues
TAAAPSRTVYLDHAATTPMVPAAVEAMTAHLREVGNPSSLHASGRRARRVVEESRETIAQALNCRPGEVVFTSGGTEADN
LAVKGIFWSRRDEDARRTRVLTTAVEHHAVLDAVHWLGHAEGAEVELLPVDDRARLDVDALRSAIERDPASVAVVSVMWA
NNEVGTLQPIHDVVALAAEHGIPVHTDAVQAVGHVPVDFAASGIDALTLTGHKLGGPYGVGALVVRREVPVRPLVHGGGQ
ERDIRSGTLDTPAIAGFAAAVELAVKHQADHATHIGALRDELVRRVIEVVPDAHLHGAALDLGGTERLPGNAHLGFPGCE
GDSLLMLLDARGIECSTGSACSAGVPQPSHVLLAMGCDADQARHSLRFSLGHTTTAADVDAVAEAIGPVVERARAARR

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=403, Percent_Identity=38.9578163771712, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI156713448, Length=411, Percent_Identity=34.5498783454988, Blast_Score=219, Evalue=3e-57,
Organism=Escherichia coli, GI48994898, Length=398, Percent_Identity=36.1809045226131, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1787970, Length=379, Percent_Identity=26.9129287598945, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789175, Length=406, Percent_Identity=27.0935960591133, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25143064, Length=375, Percent_Identity=38.6666666666667, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17533177, Length=328, Percent_Identity=28.0487804878049, Blast_Score=127, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6319831, Length=395, Percent_Identity=38.7341772151899, Blast_Score=255, Evalue=1e-68,
Organism=Drosophila melanogaster, GI20129463, Length=392, Percent_Identity=39.7959183673469, Blast_Score=254, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41893; Mature: 41762

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAAPSRTVYLDHAATTPMVPAAVEAMTAHLREVGNPSSLHASGRRARRVVEESRETIA
CCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHH
QALNCRPGEVVFTSGGTEADNLAVKGIFWSRRDEDARRTRVLTTAVEHHAVLDAVHWLGH
HHHCCCCCCEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
AEGAEVELLPVDDRARLDVDALRSAIERDPASVAVVSVMWANNEVGTLQPIHDVVALAAE
CCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHH
HGIPVHTDAVQAVGHVPVDFAASGIDALTLTGHKLGGPYGVGALVVRREVPVRPLVHGGG
CCCCCCHHHHHHHCCCCHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCHHCCCCC
QERDIRSGTLDTPAIAGFAAAVELAVKHQADHATHIGALRDELVRRVIEVVPDAHLHGAA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
LDLGGTERLPGNAHLGFPGCEGDSLLMLLDARGIECSTGSACSAGVPQPSHVLLAMGCDA
EECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCH
DQARHSLRFSLGHTTTAADVDAVAEAIGPVVERARAARR
HHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAAAPSRTVYLDHAATTPMVPAAVEAMTAHLREVGNPSSLHASGRRARRVVEESRETIA
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHH
QALNCRPGEVVFTSGGTEADNLAVKGIFWSRRDEDARRTRVLTTAVEHHAVLDAVHWLGH
HHHCCCCCCEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
AEGAEVELLPVDDRARLDVDALRSAIERDPASVAVVSVMWANNEVGTLQPIHDVVALAAE
CCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHH
HGIPVHTDAVQAVGHVPVDFAASGIDALTLTGHKLGGPYGVGALVVRREVPVRPLVHGGG
CCCCCCHHHHHHHCCCCHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCHHCCCCC
QERDIRSGTLDTPAIAGFAAAVELAVKHQADHATHIGALRDELVRRVIEVVPDAHLHGAA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
LDLGGTERLPGNAHLGFPGCEGDSLLMLLDARGIECSTGSACSAGVPQPSHVLLAMGCDA
EECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCH
DQARHSLRFSLGHTTTAADVDAVAEAIGPVVERARAARR
HHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]