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The map label for this gene is ligA

Identifier: 119717662

GI number: 119717662

Start: 3646044

End: 3648302

Strand: Reverse

Name: ligA

Synonym: Noca_3440

Alternate gene names: 119717662

Gene position: 3648302-3646044 (Counterclockwise)

Preceding gene: 119717665

Following gene: 119717661

Centisome position: 73.17

GC content: 71.62

Gene sequence:

>2259_bases
ATGAAACGAAACGGTTTCGTTCCATCGAACAGCGTAGGTCGGCGCGGCATTCCCTCCAACTCGACTTCTTCCGCGATCGC
TTCCGCAGGCGGGGCGGTGGTCTGGACCGGAGCGGGCGCGGGCCGGGTGCGAGCACGGCCTGAGCAGGTGTCGGTCGGCC
CCGGCACAATGTCCTGCGTGACCGACCCGCGCGAGGAGCACCGCCTGATCGCCGAGGAGATCGAGGAGGCCCGGTGGCGC
TACTACGTCCTGGACAGCCCGACCATCGACGACGCCGACTTCGACCGGCGGATGCGGCGCCTGGAGGCGCTCGAGGAGGA
GTTCCCCGAGCTGCGCACCCCCGACTCGCCGACCCAGACGGTCGGCGGGGCGGTGTCGACCGACTTCACCTCCCACCCCC
ACCTGCGCCGGATGGAGAGCCTGGACAACGCCTTCTCGGTCGAGGAGGTCGAGGCGTGGTACGCCCGCCTGCGCCGGGAC
GGCGTCGAGGACCCGGCGCTGCTGTGTGAGCTCAAGGTCGACGGCCTGGCGATCAACCTGCTCTACGAGGAGGGCCGCCT
GGTGCGGGCGCTGACCCGCGGTGACGGCACCACCGGTGAGGACGTCACCTCGAACGTCAAGACGATCACCTCGGTCCCGC
ACCGCCTCACCGGCACCGACGAGTTCCCGGTGCCCGCGCTGGTCGAGGTGCGCGGCGAGGTGTTCTTGCCCGTCGAGGCG
TTCGAACGGCTCAACGAGTCGCTGCTCGAGGCCGGCAAGGCGCCGTTCGCCAACCCCCGCAACTCCGCTGCCGGCTCGCT
GCGCCAGAAGGACCCCCGGATCACGGCGTCCCGGGCGCTGGGGATGGTCTGCCACGGCATCGGCGAGCGCCGCGGCTTCG
AGCCCCAGGCCCAGTCCCACGCCTACGACGCACTGGCCGCCTGGGGGCTGCCCACCAGCGACCAGGTGCGGGTGGTGTCG
ACCCTGAAGGGCGTCGAGGGCTACATCGAGAACGCCGGCGCCAGGCGGCACACGATCGTGCCGTACGAGATCGACGGCGT
CGTCGTGAAGGTCGACGACGTGGCGCTCCAGCGCCGCCTCGGCTCGACCAGCCGGGCCCCGCGCTGGGCGATCGCGTTCA
AGTACCCGCCCGAGGAGGTCAACGCCAAGCTCCTCGAGATCCGGGTGAACGTCGGGCGCACGGGCCGGGTCACGCCGTAC
GCCGTGATGGAGCCGACCAAGGTCGCCGGCTCGACCGTGGAGAACGCGACGCTGCACAACTTCTACGAGGTCGAGCGCAA
GGACGTCCGGCCCGGCGGCCCCGGTGACCCCGGTGACACGGTGATCCTGCGCAAGGCCGGGGACGTGATCCCCGAGATCC
TCGGCCCGGTCCTGGCGCTGCGGCCCGAGGGGCTGCAGCCGTGGGTGCCGCCGACGACCTGCCCGTCCTGCGGCACGCCC
CTGGTGGAGCAGAAGGAGGGCGACAAGGACCGGCGCTGCCCCAACCACGAGAAGTGCCCGGGCCAGCTGCGTGAGCGGGT
CTTCTTCGTCGCCAGCCGCAACGCGTTCGACATCGAGGGGCTCGGCTACGAGGCCGCGGTCGCGCTGCTCGACGCCGAGG
TGATCGCCAACGAGGGCGACGTGTTCGACCTGGACGCGGCCGCGCTGCTCCGCGCGCCGCTGTTCACCCGGGCGCCGAAG
AAGGACGAGGACGGACCCCAGCTGAGTGCGAACGGCCAGCGGCTGCTCGACAACCTCGACAAGGCGAAGCAGGTGCCGCT
GTGGCGGGTACTGGTCGCGCTGTCGATCCGGCACGTCGGGCCGAAGGCCGGGCGGGCGCTGGCGACGGAGTTCGGCTCCA
TGGCGGCGATCCGGGCCGCGACGGAGGAGCAGCTGGCCGCGGCCGAGGGCGTCGGCCCGACCATCGCCGAGGCCGTGATC
GAGTGGTTCAAGGAGCCGTGGCACGTCGAGATCGTCGACAAGTGGGAGCGCGCCGGCGTCACCATGGCGGACGAGCGCGA
CGAGTCCACGCCGCGGACGTTGGCGGGGCTGACCGTCGTGGTCACCGGCTCCCTGGTCGACTTCAGCCGTGACTCGGCCA
AGGAGGCGATCCTCTCGCGCGGTGGCAAGGCCGCGGGCTCGGTGTCGAAGAAGACCGACTACGTCGTGGTGGGGGAGAAC
GCCGGGTCCAAGGCCGACAAGGCCGAGCAGCTCGGCGTCCCCGTCCTCGACGAGGCCGGCTTCAAGACGCTGCTGGACGG
CGGACCCGCGGCCCTGTGA

Upstream 100 bases:

>100_bases
CACCTCCAACGTCGCGTCGAGGATCTCCTGCTCACGGTCGCCCTCGACCCGGGGGCGCTCGATCGGGGTCATCGCTGTCC
TTCGCCTGCCGTTCACCTGG

Downstream 100 bases:

>100_bases
CGCCGCCTGTGACAGATGGCCCTGGGCTCGGGACGTACGCCTCTCGTGACCTAGGTCTCATCGGCCCATGCTCGGTGCCA
TCGGAGTCATCCGTCTCGCA

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 752; Mature: 752

Protein sequence:

>752_residues
MKRNGFVPSNSVGRRGIPSNSTSSAIASAGGAVVWTGAGAGRVRARPEQVSVGPGTMSCVTDPREEHRLIAEEIEEARWR
YYVLDSPTIDDADFDRRMRRLEALEEEFPELRTPDSPTQTVGGAVSTDFTSHPHLRRMESLDNAFSVEEVEAWYARLRRD
GVEDPALLCELKVDGLAINLLYEEGRLVRALTRGDGTTGEDVTSNVKTITSVPHRLTGTDEFPVPALVEVRGEVFLPVEA
FERLNESLLEAGKAPFANPRNSAAGSLRQKDPRITASRALGMVCHGIGERRGFEPQAQSHAYDALAAWGLPTSDQVRVVS
TLKGVEGYIENAGARRHTIVPYEIDGVVVKVDDVALQRRLGSTSRAPRWAIAFKYPPEEVNAKLLEIRVNVGRTGRVTPY
AVMEPTKVAGSTVENATLHNFYEVERKDVRPGGPGDPGDTVILRKAGDVIPEILGPVLALRPEGLQPWVPPTTCPSCGTP
LVEQKEGDKDRRCPNHEKCPGQLRERVFFVASRNAFDIEGLGYEAAVALLDAEVIANEGDVFDLDAAALLRAPLFTRAPK
KDEDGPQLSANGQRLLDNLDKAKQVPLWRVLVALSIRHVGPKAGRALATEFGSMAAIRAATEEQLAAAEGVGPTIAEAVI
EWFKEPWHVEIVDKWERAGVTMADERDESTPRTLAGLTVVVTGSLVDFSRDSAKEAILSRGGKAAGSVSKKTDYVVVGEN
AGSKADKAEQLGVPVLDEAGFKTLLDGGPAAL

Sequences:

>Translated_752_residues
MKRNGFVPSNSVGRRGIPSNSTSSAIASAGGAVVWTGAGAGRVRARPEQVSVGPGTMSCVTDPREEHRLIAEEIEEARWR
YYVLDSPTIDDADFDRRMRRLEALEEEFPELRTPDSPTQTVGGAVSTDFTSHPHLRRMESLDNAFSVEEVEAWYARLRRD
GVEDPALLCELKVDGLAINLLYEEGRLVRALTRGDGTTGEDVTSNVKTITSVPHRLTGTDEFPVPALVEVRGEVFLPVEA
FERLNESLLEAGKAPFANPRNSAAGSLRQKDPRITASRALGMVCHGIGERRGFEPQAQSHAYDALAAWGLPTSDQVRVVS
TLKGVEGYIENAGARRHTIVPYEIDGVVVKVDDVALQRRLGSTSRAPRWAIAFKYPPEEVNAKLLEIRVNVGRTGRVTPY
AVMEPTKVAGSTVENATLHNFYEVERKDVRPGGPGDPGDTVILRKAGDVIPEILGPVLALRPEGLQPWVPPTTCPSCGTP
LVEQKEGDKDRRCPNHEKCPGQLRERVFFVASRNAFDIEGLGYEAAVALLDAEVIANEGDVFDLDAAALLRAPLFTRAPK
KDEDGPQLSANGQRLLDNLDKAKQVPLWRVLVALSIRHVGPKAGRALATEFGSMAAIRAATEEQLAAAEGVGPTIAEAVI
EWFKEPWHVEIVDKWERAGVTMADERDESTPRTLAGLTVVVTGSLVDFSRDSAKEAILSRGGKAAGSVSKKTDYVVVGEN
AGSKADKAEQLGVPVLDEAGFKTLLDGGPAAL
>Mature_752_residues
MKRNGFVPSNSVGRRGIPSNSTSSAIASAGGAVVWTGAGAGRVRARPEQVSVGPGTMSCVTDPREEHRLIAEEIEEARWR
YYVLDSPTIDDADFDRRMRRLEALEEEFPELRTPDSPTQTVGGAVSTDFTSHPHLRRMESLDNAFSVEEVEAWYARLRRD
GVEDPALLCELKVDGLAINLLYEEGRLVRALTRGDGTTGEDVTSNVKTITSVPHRLTGTDEFPVPALVEVRGEVFLPVEA
FERLNESLLEAGKAPFANPRNSAAGSLRQKDPRITASRALGMVCHGIGERRGFEPQAQSHAYDALAAWGLPTSDQVRVVS
TLKGVEGYIENAGARRHTIVPYEIDGVVVKVDDVALQRRLGSTSRAPRWAIAFKYPPEEVNAKLLEIRVNVGRTGRVTPY
AVMEPTKVAGSTVENATLHNFYEVERKDVRPGGPGDPGDTVILRKAGDVIPEILGPVLALRPEGLQPWVPPTTCPSCGTP
LVEQKEGDKDRRCPNHEKCPGQLRERVFFVASRNAFDIEGLGYEAAVALLDAEVIANEGDVFDLDAAALLRAPLFTRAPK
KDEDGPQLSANGQRLLDNLDKAKQVPLWRVLVALSIRHVGPKAGRALATEFGSMAAIRAATEEQLAAAEGVGPTIAEAVI
EWFKEPWHVEIVDKWERAGVTMADERDESTPRTLAGLTVVVTGSLVDFSRDSAKEAILSRGGKAAGSVSKKTDYVVVGEN
AGSKADKAEQLGVPVLDEAGFKTLLDGGPAAL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=77, Percent_Identity=48.0519480519481, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1788750, Length=677, Percent_Identity=43.5745937961595, Blast_Score=490, Evalue=1e-139,
Organism=Escherichia coli, GI87082305, Length=585, Percent_Identity=23.7606837606838, Blast_Score=95, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_NOCSJ (A1SMA5)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_924627.1
- ProteinModelPortal:   A1SMA5
- STRING:   A1SMA5
- GeneID:   4597256
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_3440
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- BioCyc:   NSP35761:NOCA_3440-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 81362; Mature: 81362

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 172-172 BINDING 170-170 BINDING 193-193 BINDING 233-233 BINDING 350-350 BINDING 374-374

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNGFVPSNSVGRRGIPSNSTSSAIASAGGAVVWTGAGAGRVRARPEQVSVGPGTMSCV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCEEECCCCCEECC
TDPREEHRLIAEEIEEARWRYYVLDSPTIDDADFDRRMRRLEALEEEFPELRTPDSPTQT
CCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VGGAVSTDFTSHPHLRRMESLDNAFSVEEVEAWYARLRRDGVEDPALLCELKVDGLAINL
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCEEEEE
LYEEGRLVRALTRGDGTTGEDVTSNVKTITSVPHRLTGTDEFPVPALVEVRGEVFLPVEA
EECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHEECCCCEEECHHH
FERLNESLLEAGKAPFANPRNSAAGSLRQKDPRITASRALGMVCHGIGERRGFEPQAQSH
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
AYDALAAWGLPTSDQVRVVSTLKGVEGYIENAGARRHTIVPYEIDGVVVKVDDVALQRRL
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCEEEEEHHHHHHHHH
GSTSRAPRWAIAFKYPPEEVNAKLLEIRVNVGRTGRVTPYAVMEPTKVAGSTVENATLHN
CCCCCCCEEEEEEECCHHHHCCEEEEEEEECCCCCCCCCEEECCCHHHCCCCCCCCHHHH
FYEVERKDVRPGGPGDPGDTVILRKAGDVIPEILGPVLALRPEGLQPWVPPTTCPSCGTP
HHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
LVEQKEGDKDRRCPNHEKCPGQLRERVFFVASRNAFDIEGLGYEAAVALLDAEVIANEGD
CCCCCCCCHHCCCCCCCCCCHHHHHHEEEEECCCCCEECCCCHHHHHHHHHHHHHCCCCC
VFDLDAAALLRAPLFTRAPKKDEDGPQLSANGQRLLDNLDKAKQVPLWRVLVALSIRHVG
EEECCHHHHHHCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
PKAGRALATEFGSMAAIRAATEEQLAAAEGVGPTIAEAVIEWFKEPWHVEIVDKWERAGV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEHHHHCCC
TMADERDESTPRTLAGLTVVVTGSLVDFSRDSAKEAILSRGGKAAGSVSKKTDYVVVGEN
EEECCCCCCCCHHHHCEEEEEECCHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECC
AGSKADKAEQLGVPVLDEAGFKTLLDGGPAAL
CCCCCCHHHHCCCCCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MKRNGFVPSNSVGRRGIPSNSTSSAIASAGGAVVWTGAGAGRVRARPEQVSVGPGTMSCV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCEEECCCCCEECC
TDPREEHRLIAEEIEEARWRYYVLDSPTIDDADFDRRMRRLEALEEEFPELRTPDSPTQT
CCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VGGAVSTDFTSHPHLRRMESLDNAFSVEEVEAWYARLRRDGVEDPALLCELKVDGLAINL
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCEEEEE
LYEEGRLVRALTRGDGTTGEDVTSNVKTITSVPHRLTGTDEFPVPALVEVRGEVFLPVEA
EECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHEECCCCEEECHHH
FERLNESLLEAGKAPFANPRNSAAGSLRQKDPRITASRALGMVCHGIGERRGFEPQAQSH
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
AYDALAAWGLPTSDQVRVVSTLKGVEGYIENAGARRHTIVPYEIDGVVVKVDDVALQRRL
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCEEEEEHHHHHHHHH
GSTSRAPRWAIAFKYPPEEVNAKLLEIRVNVGRTGRVTPYAVMEPTKVAGSTVENATLHN
CCCCCCCEEEEEEECCHHHHCCEEEEEEEECCCCCCCCCEEECCCHHHCCCCCCCCHHHH
FYEVERKDVRPGGPGDPGDTVILRKAGDVIPEILGPVLALRPEGLQPWVPPTTCPSCGTP
HHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
LVEQKEGDKDRRCPNHEKCPGQLRERVFFVASRNAFDIEGLGYEAAVALLDAEVIANEGD
CCCCCCCCHHCCCCCCCCCCHHHHHHEEEEECCCCCEECCCCHHHHHHHHHHHHHCCCCC
VFDLDAAALLRAPLFTRAPKKDEDGPQLSANGQRLLDNLDKAKQVPLWRVLVALSIRHVG
EEECCHHHHHHCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
PKAGRALATEFGSMAAIRAATEEQLAAAEGVGPTIAEAVIEWFKEPWHVEIVDKWERAGV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEHHHHCCC
TMADERDESTPRTLAGLTVVVTGSLVDFSRDSAKEAILSRGGKAAGSVSKKTDYVVVGEN
EEECCCCCCCCHHHHCEEEEEECCHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECC
AGSKADKAEQLGVPVLDEAGFKTLLDGGPAAL
CCCCCCHHHHCCCCCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA