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The map label for this gene is thiC [H]

Identifier: 119717622

GI number: 119717622

Start: 3603372

End: 3605057

Strand: Reverse

Name: thiC [H]

Synonym: Noca_3400

Alternate gene names: 119717622

Gene position: 3605057-3603372 (Counterclockwise)

Preceding gene: 119717623

Following gene: 119717620

Centisome position: 72.31

GC content: 68.68

Gene sequence:

>1686_bases
ATGCAGATCCACCCGAGCCACAGCACCGTCCACCGCACCGGATCGCGCGCCGACATCCGCGTGCCCTTCACCCGGGTCGC
GCTCACCAACGGCGAGACGTTCGACCGGTACGCCACTGCGGGACCCGGCAGCGACCCCGAGGTCGGGCTGCCGCCCCTGC
GCGCTGACTGGATCGCCCAGCGCGGCGACACCGAGGAGTACGCCGGCCGGGAGACCCAGCTGCTCGACAACGGCAGGGCC
GCCATCCGACGGGGCGAGGCACGCGACCAGTGGCGAGGGACCAGAAGCCGGCCGCGGCGCGGTACCAGCACCGTCACCCA
GATGCACTACGCCCGGCAGGGCGTGGTGACCCCGGAGATGGAGTACGTCGCGATCCGCGAGGGCTGCAACGTCGACCTGG
TCCGCTCCGAGGTCGCCGCCGGGCGCGCGATCATCCCCGCCAACCTCAACCACCCCGAGGCCGAGCCGATGATCATCGGG
CGCCGGTTCCTGGTCAAGGTGAACGCCAACATCGGCAATTCCGCGGTCACCAGCTCGATCGCCGAGGAGGTCGACAAGCT
CACCTGGGCGGTCACCTGGGGTGCCGACACCGTCATGGACCTGTCCACCGGCGACGACATCCACACCACCCGGGAATGGA
TCATCCGCAACTCACCGGTCCCGATCGGGACCGTCCCGATCTACCAGGCCCTGGAGAAGGTCGACGGCGACGCCAGCCGG
CTGACCTGGGAGATCTTCCGAGACACCGTCATCGAGCAGTGCGAACAGGGCGTGGACTACATGACCATCCATGCCGGGGT
GCTGCTGCGCTACGTGCCACTGACCGCGCAGCGCATCACCGGGATCGTCTCCCGCGGCGGGTCGATCATGGCCGGCTGGT
GCCTGGCGCACCACCAGGAGAACTTCCTCTACACGCACTTCGACGAGCTGTGCGAGATCTTCGCACGCTACGACGTGTCC
TTCTCGCTCGGCGACGGCCTGCGCCCCGGTTGCACAGCCGATGCGAACGACGAGGCGCAGCTCTCCGAGCTGCGTACCCT
GGCCGAGCTCACCCAGCGTGCCTGGGAGCACGACGTCCAGGTGATGGTGGAAGGACCTGGGCACGTGCCGCTCAACCTGG
TCGAGGAGAACGTCGTCCTGCAGCAGGACTGGTGCCACGGCGCCCCGTTCTACACCCTCGGCCCGCTGGCCACCGACATC
GCACCCGGCTACGACCACATCACCTCCGCGATCGGCGCGGCGGCCATCGCCATGCACGGCACCGCCATGCTCTGCTACGT
CACCCCCAAGGAGCACCTCGGACTGCCGAACCGCGACGACGTCAAGACCGGCGTGATCACCTACAAGCTCTCCGCGCACG
CCGCCGACGTCGCCAAGGGCCACCCCGGAGCCCGCGACTGGGACGACGCCTTGTCCAAGGCCCGCTTCGAGTTCCGCTGG
CACGACCAGTTCGCCCTCTCCCTCGACCCGCACACCGCCGAGTCCTTCCACGACGAGACGCTCCCGGCCGAGGCCAGCAA
GACGGCGCACTTCTGCTCCATGTGCGGCCCGAAGTTCTGCTCGATGCGCATCAGCCAGGACGTACGCGACTACGTCACCT
CAGGCATGGCCGAGAAGTCGGCGCAGTTCCTGGAGCTGGGCTCCTCGGTCTACGTCGAGGGTGATACCTACACCGCGGCA
CCGTGA

Upstream 100 bases:

>100_bases
CACTGCGCCGCAGCGCCGGCTGGCGCCTGGGCCGCGGTCGCGGCCCCGTCGCCCACACCTTTGCCCATCCCACGACGAAC
CCATCCCCACAGGAGGCGTG

Downstream 100 bases:

>100_bases
CCTGACAGCGGCCGATACCCCTCATCGCCGGCCAGACCTCGGCAGCCATTCCTCGTGGTCTCCGAACCCGGGACGCGGGA
GGGGCTGTCCGGATGGCCCG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MQIHPSHSTVHRTGSRADIRVPFTRVALTNGETFDRYATAGPGSDPEVGLPPLRADWIAQRGDTEEYAGRETQLLDNGRA
AIRRGEARDQWRGTRSRPRRGTSTVTQMHYARQGVVTPEMEYVAIREGCNVDLVRSEVAAGRAIIPANLNHPEAEPMIIG
RRFLVKVNANIGNSAVTSSIAEEVDKLTWAVTWGADTVMDLSTGDDIHTTREWIIRNSPVPIGTVPIYQALEKVDGDASR
LTWEIFRDTVIEQCEQGVDYMTIHAGVLLRYVPLTAQRITGIVSRGGSIMAGWCLAHHQENFLYTHFDELCEIFARYDVS
FSLGDGLRPGCTADANDEAQLSELRTLAELTQRAWEHDVQVMVEGPGHVPLNLVEENVVLQQDWCHGAPFYTLGPLATDI
APGYDHITSAIGAAAIAMHGTAMLCYVTPKEHLGLPNRDDVKTGVITYKLSAHAADVAKGHPGARDWDDALSKARFEFRW
HDQFALSLDPHTAESFHDETLPAEASKTAHFCSMCGPKFCSMRISQDVRDYVTSGMAEKSAQFLELGSSVYVEGDTYTAA
P

Sequences:

>Translated_561_residues
MQIHPSHSTVHRTGSRADIRVPFTRVALTNGETFDRYATAGPGSDPEVGLPPLRADWIAQRGDTEEYAGRETQLLDNGRA
AIRRGEARDQWRGTRSRPRRGTSTVTQMHYARQGVVTPEMEYVAIREGCNVDLVRSEVAAGRAIIPANLNHPEAEPMIIG
RRFLVKVNANIGNSAVTSSIAEEVDKLTWAVTWGADTVMDLSTGDDIHTTREWIIRNSPVPIGTVPIYQALEKVDGDASR
LTWEIFRDTVIEQCEQGVDYMTIHAGVLLRYVPLTAQRITGIVSRGGSIMAGWCLAHHQENFLYTHFDELCEIFARYDVS
FSLGDGLRPGCTADANDEAQLSELRTLAELTQRAWEHDVQVMVEGPGHVPLNLVEENVVLQQDWCHGAPFYTLGPLATDI
APGYDHITSAIGAAAIAMHGTAMLCYVTPKEHLGLPNRDDVKTGVITYKLSAHAADVAKGHPGARDWDDALSKARFEFRW
HDQFALSLDPHTAESFHDETLPAEASKTAHFCSMCGPKFCSMRISQDVRDYVTSGMAEKSAQFLELGSSVYVEGDTYTAA
P
>Mature_561_residues
MQIHPSHSTVHRTGSRADIRVPFTRVALTNGETFDRYATAGPGSDPEVGLPPLRADWIAQRGDTEEYAGRETQLLDNGRA
AIRRGEARDQWRGTRSRPRRGTSTVTQMHYARQGVVTPEMEYVAIREGCNVDLVRSEVAAGRAIIPANLNHPEAEPMIIG
RRFLVKVNANIGNSAVTSSIAEEVDKLTWAVTWGADTVMDLSTGDDIHTTREWIIRNSPVPIGTVPIYQALEKVDGDASR
LTWEIFRDTVIEQCEQGVDYMTIHAGVLLRYVPLTAQRITGIVSRGGSIMAGWCLAHHQENFLYTHFDELCEIFARYDVS
FSLGDGLRPGCTADANDEAQLSELRTLAELTQRAWEHDVQVMVEGPGHVPLNLVEENVVLQQDWCHGAPFYTLGPLATDI
APGYDHITSAIGAAAIAMHGTAMLCYVTPKEHLGLPNRDDVKTGVITYKLSAHAADVAKGHPGARDWDDALSKARFEFRW
HDQFALSLDPHTAESFHDETLPAEASKTAHFCSMCGPKFCSMRISQDVRDYVTSGMAEKSAQFLELGSSVYVEGDTYTAA
P

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=607, Percent_Identity=60.1317957166392, Blast_Score=729, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 61856; Mature: 61856

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIHPSHSTVHRTGSRADIRVPFTRVALTNGETFDRYATAGPGSDPEVGLPPLRADWIAQ
CCCCCCCCHHHCCCCCCEEECCEEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCHHHHHC
RGDTEEYAGRETQLLDNGRAAIRRGEARDQWRGTRSRPRRGTSTVTQMHYARQGVVTPEM
CCCCHHHCCCCHHHHHCCHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
EYVAIREGCNVDLVRSEVAAGRAIIPANLNHPEAEPMIIGRRFLVKVNANIGNSAVTSSI
EEEEECCCCCEEEHHHHHHCCCEEEECCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHH
AEEVDKLTWAVTWGADTVMDLSTGDDIHTTREWIIRNSPVPIGTVPIYQALEKVDGDASR
HHHHHHEEEEEEECCCEEEECCCCCCCHHHHHHHEECCCCCCCCCHHHHHHHHCCCCHHH
LTWEIFRDTVIEQCEQGVDYMTIHAGVLLRYVPLTAQRITGIVSRGGSIMAGWCLAHHQE
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCC
NFLYTHFDELCEIFARYDVSFSLGDGLRPGCTADANDEAQLSELRTLAELTQRAWEHDVQ
CCEEHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEE
VMVEGPGHVPLNLVEENVVLQQDWCHGAPFYTLGPLATDIAPGYDHITSAIGAAAIAMHG
EEEECCCCCCCEEECCCEEEEECCCCCCCCEEECCHHHHCCCCHHHHHHHHHHHHHHCCC
TAMLCYVTPKEHLGLPNRDDVKTGVITYKLSAHAADVAKGHPGARDWDDALSKARFEFRW
CEEEEEECCHHHCCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCCHHHHHHHCEEEEEE
HDQFALSLDPHTAESFHDETLPAEASKTAHFCSMCGPKFCSMRISQDVRDYVTSGMAEKS
CCEEEEEECCCCHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHH
AQFLELGSSVYVEGDTYTAAP
HHHHHCCCEEEEECCEEECCC
>Mature Secondary Structure
MQIHPSHSTVHRTGSRADIRVPFTRVALTNGETFDRYATAGPGSDPEVGLPPLRADWIAQ
CCCCCCCCHHHCCCCCCEEECCEEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCHHHHHC
RGDTEEYAGRETQLLDNGRAAIRRGEARDQWRGTRSRPRRGTSTVTQMHYARQGVVTPEM
CCCCHHHCCCCHHHHHCCHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
EYVAIREGCNVDLVRSEVAAGRAIIPANLNHPEAEPMIIGRRFLVKVNANIGNSAVTSSI
EEEEECCCCCEEEHHHHHHCCCEEEECCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHH
AEEVDKLTWAVTWGADTVMDLSTGDDIHTTREWIIRNSPVPIGTVPIYQALEKVDGDASR
HHHHHHEEEEEEECCCEEEECCCCCCCHHHHHHHEECCCCCCCCCHHHHHHHHCCCCHHH
LTWEIFRDTVIEQCEQGVDYMTIHAGVLLRYVPLTAQRITGIVSRGGSIMAGWCLAHHQE
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCC
NFLYTHFDELCEIFARYDVSFSLGDGLRPGCTADANDEAQLSELRTLAELTQRAWEHDVQ
CCEEHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEE
VMVEGPGHVPLNLVEENVVLQQDWCHGAPFYTLGPLATDIAPGYDHITSAIGAAAIAMHG
EEEECCCCCCCEEECCCEEEEECCCCCCCCEEECCHHHHCCCCHHHHHHHHHHHHHHCCC
TAMLCYVTPKEHLGLPNRDDVKTGVITYKLSAHAADVAKGHPGARDWDDALSKARFEFRW
CEEEEEECCHHHCCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCCHHHHHHHCEEEEEE
HDQFALSLDPHTAESFHDETLPAEASKTAHFCSMCGPKFCSMRISQDVRDYVTSGMAEKS
CCEEEEEECCCCHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHH
AQFLELGSSVYVEGDTYTAAP
HHHHHCCCEEEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910 [H]