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The map label for this gene is ilvB1 [H]

Identifier: 119717615

GI number: 119717615

Start: 3595120

End: 3596877

Strand: Reverse

Name: ilvB1 [H]

Synonym: Noca_3392

Alternate gene names: 119717615

Gene position: 3596877-3595120 (Counterclockwise)

Preceding gene: 119717616

Following gene: 119717614

Centisome position: 72.14

GC content: 70.53

Gene sequence:

>1758_bases
ATGAGCGAGCAGATGACAGGGGCCCAGAGCCTGGTCAGGTCGCTGGAGGCAGTGGGCACCGAGCACATCTTCGGAATCCC
GGGCGGCGCCATCCTCCCGGCGTACGACCCGCTGATGGACTCCTCGATCCGGCACATCCTGGTCCGCCACGAGCAGGGCG
CCGGCCACGCCGCCCAGGGGTACGCCGCCGCCACCGGCCGGGTCGGGGTCTGCATGGCCACCTCCGGTCCGGGTGCGACC
AACCTGGTCACGCCGCTCGCCGACGCGCACATGGACTCGGTGCCGATCGTGGCGGTCACCGGCCAGGTCGGCGCCGCCCA
GATCGGCACCGACGCCTTCCAGGAGGCCGACATCCGCGGCATCACGATGCCGATCACCAAGCACAACTTCCTGGTCACCG
ACCCCGCCGAGATCCCGCGCACGATCGCCGAGGCCTTCCACATCGCCTCGACCGGCCGCCCGGGCCCGGTGCTCGTCGAC
GTCGCCAAGTCGGCGCTGCAGGCGGCGACCGACTTCGCGTGGCCCACCGAGCTCGCGCTTCCCGGCTACCGACCCGTGAC
CAGGCCGCACTCCAAGCAGATCCGCGAGGCCGCCAAGCTGATGCTGGAGTCGCGCCGACCGGTGCTCTACGTCGGCGGCG
GCACGATCCGGGCCGGCGCCTCGAAGGAGCTGCGTGTGCTCGCGGAGCTCACCGGCATCCCGGTGGTGACGACCCTGATG
GCCCGGGGGGCGTTCCCCGACAGCCACCCGCAGCACCTCGGCATGCCCGGCATGCACGGCACGGTCGCCGCCGTGGCGGG
CCTGCAGCGCAGCGACCTGATCATCAGCCTGGGCGCCCGCTTCGACGACCGGGTGACCGGCAAGCTCGACTCGTTCGCCC
CCGGCGCCCGGGTCATCCACGCCGACATCGACCCGGCCGAGATCGGCAAGAACCGCGCGGTCGACGTCCCGATCGTCGGC
GACTGTCGGGAGGTGATCAGCGACCTGGTCGTCGCCCTCAAGGCCGAGGCCGACGCCGGTCACACCGGCGACTACGAGGC
CTGGGTGGACTTCCTGGCCGGGGTGAAGAAGAAGTACCCGCTCGGGTACGACGCCCCCACCGACGGCGGCCTGGCCCCGC
AGTACGTCCTCGAGCGGCTCGGCGCGATCGCCGGCCCGGAGGCCATCTACTGCGCCGGCGTCGGCCAGCACCAGATGTGG
GCCGCGCACTTCGTCGGCTACGAGAACCCGAACACCTGGATCAACTCCGGCGGACTGGGCACCATGGGCTTCTCCGTGCC
CGCCGCGATGGGCGCCAAGGTCGGCCGCCCCGACGCGACCGTGTGGTCGATCGACGGCGACGGGTGCTTCCAGATGACCA
ACCAGGAGCTCGCCACCTGCGCGATCAACGACATCCCGATCAAGGTCGCGATCATCAACAACGAGTCGCTGGGCATGGTC
CGGCAGTGGCAGACGCTGTTCTACAACGAGCGCTACTCCAACACCGATCTGCACTCCAAGCGGGTCCCAGACTTCGTCAA
GCTGGCCGACGCCTACGGCTGCGTGGGCCTGTCCTGCGACAGCCCGGACGACGTGGACGCCACGATCCGCAAGGCGATGG
AGATCGACGACGCCCCCGTCGTGGTGGACTTCCGGGTGCACCGCGACGCGATGGTCTGGCCGATGGTCGCCGCCGGCACC
AGCAACGACGAGATCAAGTACGCCCGCAACCTGGCCCCGAAGTTCGACGACGACGCAGATCTGGAGCTCCGGCCGTGA

Upstream 100 bases:

>100_bases
ACGACCGCAGCGCACACGTCACCCCTCGCCGCCCACCCGGGCCGAGGGGTTTTTTGTTGGGCAGGGCACGGCACCAGGTA
GCGAGACAGGACAGACAACG

Downstream 100 bases:

>100_bases
CGACCTCCGCCAACACCCGACACACCCTGTCCGTCCTGGTGGAGAACAAGCCCGGCGTCCTCGCCCGGATCGCGGGCCTG
TTCAGCCGTCGCGGCTTCAA

Product: acetolactate synthase 1 catalytic subunit

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase large subunit; AHAS [H]

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MSEQMTGAQSLVRSLEAVGTEHIFGIPGGAILPAYDPLMDSSIRHILVRHEQGAGHAAQGYAAATGRVGVCMATSGPGAT
NLVTPLADAHMDSVPIVAVTGQVGAAQIGTDAFQEADIRGITMPITKHNFLVTDPAEIPRTIAEAFHIASTGRPGPVLVD
VAKSALQAATDFAWPTELALPGYRPVTRPHSKQIREAAKLMLESRRPVLYVGGGTIRAGASKELRVLAELTGIPVVTTLM
ARGAFPDSHPQHLGMPGMHGTVAAVAGLQRSDLIISLGARFDDRVTGKLDSFAPGARVIHADIDPAEIGKNRAVDVPIVG
DCREVISDLVVALKAEADAGHTGDYEAWVDFLAGVKKKYPLGYDAPTDGGLAPQYVLERLGAIAGPEAIYCAGVGQHQMW
AAHFVGYENPNTWINSGGLGTMGFSVPAAMGAKVGRPDATVWSIDGDGCFQMTNQELATCAINDIPIKVAIINNESLGMV
RQWQTLFYNERYSNTDLHSKRVPDFVKLADAYGCVGLSCDSPDDVDATIRKAMEIDDAPVVVDFRVHRDAMVWPMVAAGT
SNDEIKYARNLAPKFDDDADLELRP

Sequences:

>Translated_585_residues
MSEQMTGAQSLVRSLEAVGTEHIFGIPGGAILPAYDPLMDSSIRHILVRHEQGAGHAAQGYAAATGRVGVCMATSGPGAT
NLVTPLADAHMDSVPIVAVTGQVGAAQIGTDAFQEADIRGITMPITKHNFLVTDPAEIPRTIAEAFHIASTGRPGPVLVD
VAKSALQAATDFAWPTELALPGYRPVTRPHSKQIREAAKLMLESRRPVLYVGGGTIRAGASKELRVLAELTGIPVVTTLM
ARGAFPDSHPQHLGMPGMHGTVAAVAGLQRSDLIISLGARFDDRVTGKLDSFAPGARVIHADIDPAEIGKNRAVDVPIVG
DCREVISDLVVALKAEADAGHTGDYEAWVDFLAGVKKKYPLGYDAPTDGGLAPQYVLERLGAIAGPEAIYCAGVGQHQMW
AAHFVGYENPNTWINSGGLGTMGFSVPAAMGAKVGRPDATVWSIDGDGCFQMTNQELATCAINDIPIKVAIINNESLGMV
RQWQTLFYNERYSNTDLHSKRVPDFVKLADAYGCVGLSCDSPDDVDATIRKAMEIDDAPVVVDFRVHRDAMVWPMVAAGT
SNDEIKYARNLAPKFDDDADLELRP
>Mature_584_residues
SEQMTGAQSLVRSLEAVGTEHIFGIPGGAILPAYDPLMDSSIRHILVRHEQGAGHAAQGYAAATGRVGVCMATSGPGATN
LVTPLADAHMDSVPIVAVTGQVGAAQIGTDAFQEADIRGITMPITKHNFLVTDPAEIPRTIAEAFHIASTGRPGPVLVDV
AKSALQAATDFAWPTELALPGYRPVTRPHSKQIREAAKLMLESRRPVLYVGGGTIRAGASKELRVLAELTGIPVVTTLMA
RGAFPDSHPQHLGMPGMHGTVAAVAGLQRSDLIISLGARFDDRVTGKLDSFAPGARVIHADIDPAEIGKNRAVDVPIVGD
CREVISDLVVALKAEADAGHTGDYEAWVDFLAGVKKKYPLGYDAPTDGGLAPQYVLERLGAIAGPEAIYCAGVGQHQMWA
AHFVGYENPNTWINSGGLGTMGFSVPAAMGAKVGRPDATVWSIDGDGCFQMTNQELATCAINDIPIKVAIINNESLGMVR
QWQTLFYNERYSNTDLHSKRVPDFVKLADAYGCVGLSCDSPDDVDATIRKAMEIDDAPVVVDFRVHRDAMVWPMVAAGTS
NDEIKYARNLAPKFDDDADLELRP

Specific function: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine. Also involved in condensing pyruvate and 2-ketobutyrate to form 2-

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=568, Percent_Identity=23.943661971831, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI21361361, Length=588, Percent_Identity=26.0204081632653, Blast_Score=141, Evalue=2e-33,
Organism=Escherichia coli, GI87081685, Length=573, Percent_Identity=42.9319371727749, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI1790104, Length=564, Percent_Identity=42.1985815602837, Blast_Score=430, Evalue=1e-121,
Organism=Escherichia coli, GI1786717, Length=558, Percent_Identity=34.5878136200717, Blast_Score=315, Evalue=4e-87,
Organism=Escherichia coli, GI1787096, Length=553, Percent_Identity=28.2097649186257, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1788716, Length=541, Percent_Identity=26.9870609981516, Blast_Score=155, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17531299, Length=568, Percent_Identity=25.5281690140845, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=568, Percent_Identity=25.5281690140845, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17542570, Length=517, Percent_Identity=26.6924564796905, Blast_Score=130, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=583, Percent_Identity=42.0240137221269, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6320816, Length=483, Percent_Identity=21.9461697722567, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323163, Length=503, Percent_Identity=23.8568588469185, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321524, Length=506, Percent_Identity=23.1225296442688, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323073, Length=502, Percent_Identity=23.1075697211155, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922626, Length=562, Percent_Identity=25.8007117437722, Blast_Score=179, Evalue=6e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62196; Mature: 62065

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQMTGAQSLVRSLEAVGTEHIFGIPGGAILPAYDPLMDSSIRHILVRHEQGAGHAAQG
CCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHEEEEEECCCCCCCCCC
YAAATGRVGVCMATSGPGATNLVTPLADAHMDSVPIVAVTGQVGAAQIGTDAFQEADIRG
CEEECCCEEEEEECCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCHHHCCHHHHHCCCCE
ITMPITKHNFLVTDPAEIPRTIAEAFHIASTGRPGPVLVDVAKSALQAATDFAWPTELAL
EEEEEECCCEEEECHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
PGYRPVTRPHSKQIREAAKLMLESRRPVLYVGGGTIRAGASKELRVLAELTGIPVVTTLM
CCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCHHHHHHHHHCCCHHHHHHH
ARGAFPDSHPQHLGMPGMHGTVAAVAGLQRSDLIISLGARFDDRVTGKLDSFAPGARVIH
HHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEE
ADIDPAEIGKNRAVDVPIVGDCREVISDLVVALKAEADAGHTGDYEAWVDFLAGVKKKYP
ECCCHHHCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCC
LGYDAPTDGGLAPQYVLERLGAIAGPEAIYCAGVGQHQMWAAHFVGYENPNTWINSGGLG
CCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCEECCCCCC
TMGFSVPAAMGAKVGRPDATVWSIDGDGCFQMTNQELATCAINDIPIKVAIINNESLGMV
CCCCCCHHHHCCCCCCCCCEEEEECCCCCEEECCCHHEEEEECCCEEEEEEECCCCCCHH
RQWQTLFYNERYSNTDLHSKRVPDFVKLADAYGCVGLSCDSPDDVDATIRKAMEIDDAPV
HHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHCCEEEECCCCCCCHHHHHHHHHCCCCCCE
VVDFRVHRDAMVWPMVAAGTSNDEIKYARNLAPKFDDDADLELRP
EEEEEECCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCEECC
>Mature Secondary Structure 
SEQMTGAQSLVRSLEAVGTEHIFGIPGGAILPAYDPLMDSSIRHILVRHEQGAGHAAQG
CCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHEEEEEECCCCCCCCCC
YAAATGRVGVCMATSGPGATNLVTPLADAHMDSVPIVAVTGQVGAAQIGTDAFQEADIRG
CEEECCCEEEEEECCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCHHHCCHHHHHCCCCE
ITMPITKHNFLVTDPAEIPRTIAEAFHIASTGRPGPVLVDVAKSALQAATDFAWPTELAL
EEEEEECCCEEEECHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
PGYRPVTRPHSKQIREAAKLMLESRRPVLYVGGGTIRAGASKELRVLAELTGIPVVTTLM
CCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCHHHHHHHHHCCCHHHHHHH
ARGAFPDSHPQHLGMPGMHGTVAAVAGLQRSDLIISLGARFDDRVTGKLDSFAPGARVIH
HHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEE
ADIDPAEIGKNRAVDVPIVGDCREVISDLVVALKAEADAGHTGDYEAWVDFLAGVKKKYP
ECCCHHHCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCC
LGYDAPTDGGLAPQYVLERLGAIAGPEAIYCAGVGQHQMWAAHFVGYENPNTWINSGGLG
CCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCEECCCCCC
TMGFSVPAAMGAKVGRPDATVWSIDGDGCFQMTNQELATCAINDIPIKVAIINNESLGMV
CCCCCCHHHHCCCCCCCCCEEEEECCCCCEEECCCHHEEEEECCCEEEEEEECCCCCCHH
RQWQTLFYNERYSNTDLHSKRVPDFVKLADAYGCVGLSCDSPDDVDATIRKAMEIDDAPV
HHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHCCEEEECCCCCCCHHHHHHHHHCCCCCCE
VVDFRVHRDAMVWPMVAAGTSNDEIKYARNLAPKFDDDADLELRP
EEEEEECCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]