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The map label for this gene is prfA

Identifier: 119715986

GI number: 119715986

Start: 1862443

End: 1863516

Strand: Direct

Name: prfA

Synonym: Noca_1751

Alternate gene names: 119715986

Gene position: 1862443-1863516 (Clockwise)

Preceding gene: 119715985

Following gene: 119715987

Centisome position: 37.35

GC content: 71.23

Gene sequence:

>1074_bases
ATGTTCGAAGCCGTGGAGGGCATGCTCGCCGAGCACGCCGATCTCGAGCAGCGGCTCGGTGCGCCCGAGACGCACGCCGA
CGCCCGGCTCGCCAAGCAGCTCAACCAGCGCTACGCCGCCCTGTCCTCGATCATCGGTGCGTACCGGGAGCTGCAGCAGC
TCGACGACGACATCGAGGCCGCCCGCGAGCTCGCCCAGGAGGACCCGGCGTTCGCCGAGGAGGCCGCGGCCCTGACCGGT
AGGCGCCAGGAGGTCGAGGAGCGGCTACGTCGGCTCCTCGTCCCCCGAGACGCCGCCGACGACAAGGACGCGATCCTCGA
GGTCAAGTCCGGCGAGGGCGGTGAGGAGTCGGCCCTGTTCGCCGGCGACCTGCTCCGGATGTACACCCGCTACGCCGAGG
CCCGGGGCTGGAAGGTCGAGGTCCTCGACGCCGCCGAGTCCGACCTCGGCGGCTACAAGTCCGTGACGGTGGCCGTCAAG
GCGAAAGGGACGCCCGAGCCGGGGGAGGCGCCGTACGCGCTGCTCAAGTTCGAGGGCGGCGTCCACCGGGTCCAACGGGT
CCCCGTCACCGAGTCGCAGGGCCGGGTGCACACCAGTGCAGCCGGTGTGCTCGTGCTGCCTGAGGCCGAGCAGGTCGACG
TGCAGATCGACGAGAACGACCTGCGCATCGACGTCTTCCGCAGCAGCGGGCCCGGCGGCCAGAGCGTGAACACGACCGAC
TCCGCGGTCCGGATCACCCACCTGCCGACCGGCATCGTCGTGAGCTGCCAGAACGAGAAGAGCCAGCTGCAGAACCGCGA
GCAGGCGATGCGGATCCTGCGCTCCCGGCTGCTGGCCGCCGCCCAGGAGAAGGCCGACGCCGAGGCGGGGGAGGCCCGAC
GCAGCCAGGTCCGCACCGTCGACCGCTCCGAGCGCATCCGCACGTACAACTTCCCCGAGAACCGGATCTCCGACCACCGC
ACCGGCTACAAGGCCTACAACCTCGACCAGGTGCTCGACGGCGACCTCCAACCGGTCCTCGACTCCTGCGTCGAGGCCGA
CCTCGCCGCCCGCCTCGAGGCGCTGGAGCAGTGA

Upstream 100 bases:

>100_bases
AGTAGCTGTTCCCAGGCGCCGGTCACTCCGCACACGTGCGGGGGACCGGCGCCTGTTTCATGTGCCGGAGCCCGTCCGGT
CCACCACCGAGAGGTCGCCG

Downstream 100 bases:

>100_bases
GCGCCCGATGACCCAACGTCGCGCGGTCGTCACGGCCGCTGCCGAGCGGCTCCGCCACGCCGGTGTCCCCAGCCCCGGGC
ACGACGCCGAGGCGCTGCTC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MFEAVEGMLAEHADLEQRLGAPETHADARLAKQLNQRYAALSSIIGAYRELQQLDDDIEAARELAQEDPAFAEEAAALTG
RRQEVEERLRRLLVPRDAADDKDAILEVKSGEGGEESALFAGDLLRMYTRYAEARGWKVEVLDAAESDLGGYKSVTVAVK
AKGTPEPGEAPYALLKFEGGVHRVQRVPVTESQGRVHTSAAGVLVLPEAEQVDVQIDENDLRIDVFRSSGPGGQSVNTTD
SAVRITHLPTGIVVSCQNEKSQLQNREQAMRILRSRLLAAAQEKADAEAGEARRSQVRTVDRSERIRTYNFPENRISDHR
TGYKAYNLDQVLDGDLQPVLDSCVEADLAARLEALEQ

Sequences:

>Translated_357_residues
MFEAVEGMLAEHADLEQRLGAPETHADARLAKQLNQRYAALSSIIGAYRELQQLDDDIEAARELAQEDPAFAEEAAALTG
RRQEVEERLRRLLVPRDAADDKDAILEVKSGEGGEESALFAGDLLRMYTRYAEARGWKVEVLDAAESDLGGYKSVTVAVK
AKGTPEPGEAPYALLKFEGGVHRVQRVPVTESQGRVHTSAAGVLVLPEAEQVDVQIDENDLRIDVFRSSGPGGQSVNTTD
SAVRITHLPTGIVVSCQNEKSQLQNREQAMRILRSRLLAAAQEKADAEAGEARRSQVRTVDRSERIRTYNFPENRISDHR
TGYKAYNLDQVLDGDLQPVLDSCVEADLAARLEALEQ
>Mature_357_residues
MFEAVEGMLAEHADLEQRLGAPETHADARLAKQLNQRYAALSSIIGAYRELQQLDDDIEAARELAQEDPAFAEEAAALTG
RRQEVEERLRRLLVPRDAADDKDAILEVKSGEGGEESALFAGDLLRMYTRYAEARGWKVEVLDAAESDLGGYKSVTVAVK
AKGTPEPGEAPYALLKFEGGVHRVQRVPVTESQGRVHTSAAGVLVLPEAEQVDVQIDENDLRIDVFRSSGPGGQSVNTTD
SAVRITHLPTGIVVSCQNEKSQLQNREQAMRILRSRLLAAAQEKADAEAGEARRSQVRTVDRSERIRTYNFPENRISDHR
TGYKAYNLDQVLDGDLQPVLDSCVEADLAARLEALEQ

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=302, Percent_Identity=42.7152317880795, Blast_Score=248, Evalue=8e-66,
Organism=Homo sapiens, GI34577120, Length=346, Percent_Identity=38.150289017341, Blast_Score=238, Evalue=5e-63,
Organism=Homo sapiens, GI166795305, Length=206, Percent_Identity=43.2038834951456, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1787462, Length=363, Percent_Identity=43.801652892562, Blast_Score=293, Evalue=8e-81,
Organism=Escherichia coli, GI2367172, Length=344, Percent_Identity=36.6279069767442, Blast_Score=210, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=308, Percent_Identity=37.012987012987, Blast_Score=171, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6321295, Length=350, Percent_Identity=39.1428571428571, Blast_Score=234, Evalue=1e-62,
Organism=Drosophila melanogaster, GI19921226, Length=247, Percent_Identity=45.3441295546559, Blast_Score=217, Evalue=9e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_NOCSJ (A1SHH9)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_922951.1
- ProteinModelPortal:   A1SHH9
- SMR:   A1SHH9
- STRING:   A1SHH9
- GeneID:   4597933
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_1751
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   A1SHH9
- ProtClustDB:   PRK00591
- BioCyc:   NSP35761:NOCA_1751-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 39287; Mature: 39287

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEAVEGMLAEHADLEQRLGAPETHADARLAKQLNQRYAALSSIIGAYRELQQLDDDIEA
CCHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARELAQEDPAFAEEAAALTGRRQEVEERLRRLLVPRDAADDKDAILEVKSGEGGEESALF
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHEEEEECCCCCCCHHHH
AGDLLRMYTRYAEARGWKVEVLDAAESDLGGYKSVTVAVKAKGTPEPGEAPYALLKFEGG
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEEECCC
VHRVQRVPVTESQGRVHTSAAGVLVLPEAEQVDVQIDENDLRIDVFRSSGPGGQSVNTTD
HHHHHCCCCCCCCCCEEECCCCEEEECCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCC
SAVRITHLPTGIVVSCQNEKSQLQNREQAMRILRSRLLAAAQEKADAEAGEARRSQVRTV
CEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
DRSERIRTYNFPENRISDHRTGYKAYNLDQVLDGDLQPVLDSCVEADLAARLEALEQ
HHHCCCEECCCCHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFEAVEGMLAEHADLEQRLGAPETHADARLAKQLNQRYAALSSIIGAYRELQQLDDDIEA
CCHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARELAQEDPAFAEEAAALTGRRQEVEERLRRLLVPRDAADDKDAILEVKSGEGGEESALF
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHEEEEECCCCCCCHHHH
AGDLLRMYTRYAEARGWKVEVLDAAESDLGGYKSVTVAVKAKGTPEPGEAPYALLKFEGG
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEEECCC
VHRVQRVPVTESQGRVHTSAAGVLVLPEAEQVDVQIDENDLRIDVFRSSGPGGQSVNTTD
HHHHHCCCCCCCCCCEEECCCCEEEECCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCC
SAVRITHLPTGIVVSCQNEKSQLQNREQAMRILRSRLLAAAQEKADAEAGEARRSQVRTV
CEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
DRSERIRTYNFPENRISDHRTGYKAYNLDQVLDGDLQPVLDSCVEADLAARLEALEQ
HHHCCCEECCCCHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA