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The map label for this gene is sigF [H]

Identifier: 119715950

GI number: 119715950

Start: 1818821

End: 1819633

Strand: Reverse

Name: sigF [H]

Synonym: Noca_1715

Alternate gene names: 119715950

Gene position: 1819633-1818821 (Counterclockwise)

Preceding gene: 119715951

Following gene: 119715948

Centisome position: 36.5

GC content: 69.74

Gene sequence:

>813_bases
ATGTTCGCTGTCACGGGGGACGGCGACGCGGATCCGGACGCGCCGACCGGCGTCGAGCTCACGCGCCTGCGCAGTGCGGA
GCTGTTCGCGATGCTGCGCGACGACGGCGCACCGGCGGCCCGCCGGGACGCGGCCCGCGAGGGACTGGTGCACCTGCACC
TGCCGCTGGTCGAGCACTGTGCGCGCCGCTTCCGCAACCGCGGCGAGCCGCTGGAGGACCTCGTCCAGGTGGGCACGATC
GGGCTGATCAAGTCCGTCGACCGCTTCGACGCCGACCGCGGTGTGGAGTTCTCGACCTATGCGACCCCGACGATCATCGG
GGAGATCAAACGCTACTTCCGCGACAAGGGCTGGGCGATCCGGGTGCCGCGGCGGCTCCAGGAGCTGCGCATGCAGATCA
GCACGGCGTCGGCCGAGCTGACCCAGTCGCTGGGCCGGTCGCCGACGCCGCGCGAGCTCGCGGGCGCCATCGGCTGCACC
GTCGAGGAGATCGTCGAGGGCATCGAGTCGAGCAACGCCTACTCCACGCTGTCCCTGGACGCCACCGACGACGGCGACGA
CAACACCGCGAGCATGCTCGACGCGATCGGGGTCGACGACGAAGGGCTGGAGCACGTCGAGATCCGGGAGTCGCTCAAGC
CGCTGCTGGACCGGCTGGAGCCGCGGGAGAAGAAGATCCTGCTGCTGCGCTTCTTCAAGAACATGACCCAGTCGCAGATC
GCCGAGGAGATCGGCGTCTCGCAGATGCACGTCTCCCGCCTGCTCAACCGGACCCTCGAGCAGCTCCGGGTCTCCCTGGA
GGAGACGAGCTGA

Upstream 100 bases:

>100_bases
CTCACCACCTTGGCGAGCAGCGCGTCCGCGGAGGCCTCGAACGGCATCTTCTCGATCACGGTGAGCATGGAGTCGGGCCT
CCCGGGAGGCGGGATCTGAC

Downstream 100 bases:

>100_bases
GCCGGTCGCGTCAGTCCCGGTCCTCGATCCGGTCGTCGGCCAGGGCGTCAACGCTGGATGGCGCGAGCAGGCCCGCGAGC
ACGACGACGGCGACCACCGC

Product: sigma-70 region 2 domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MFAVTGDGDADPDAPTGVELTRLRSAELFAMLRDDGAPAARRDAAREGLVHLHLPLVEHCARRFRNRGEPLEDLVQVGTI
GLIKSVDRFDADRGVEFSTYATPTIIGEIKRYFRDKGWAIRVPRRLQELRMQISTASAELTQSLGRSPTPRELAGAIGCT
VEEIVEGIESSNAYSTLSLDATDDGDDNTASMLDAIGVDDEGLEHVEIRESLKPLLDRLEPREKKILLLRFFKNMTQSQI
AEEIGVSQMHVSRLLNRTLEQLRVSLEETS

Sequences:

>Translated_270_residues
MFAVTGDGDADPDAPTGVELTRLRSAELFAMLRDDGAPAARRDAAREGLVHLHLPLVEHCARRFRNRGEPLEDLVQVGTI
GLIKSVDRFDADRGVEFSTYATPTIIGEIKRYFRDKGWAIRVPRRLQELRMQISTASAELTQSLGRSPTPRELAGAIGCT
VEEIVEGIESSNAYSTLSLDATDDGDDNTASMLDAIGVDDEGLEHVEIRESLKPLLDRLEPREKKILLLRFFKNMTQSQI
AEEIGVSQMHVSRLLNRTLEQLRVSLEETS
>Mature_270_residues
MFAVTGDGDADPDAPTGVELTRLRSAELFAMLRDDGAPAARRDAAREGLVHLHLPLVEHCARRFRNRGEPLEDLVQVGTI
GLIKSVDRFDADRGVEFSTYATPTIIGEIKRYFRDKGWAIRVPRRLQELRMQISTASAELTQSLGRSPTPRELAGAIGCT
VEEIVEGIESSNAYSTLSLDATDDGDDNTASMLDAIGVDDEGLEHVEIRESLKPLLDRLEPREKKILLLRFFKNMTQSQI
AEEIGVSQMHVSRLLNRTLEQLRVSLEETS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for normal spore maturation [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=229, Percent_Identity=32.3144104803493, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1789098, Length=234, Percent_Identity=28.6324786324786, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788231, Length=227, Percent_Identity=26.8722466960352, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789871, Length=288, Percent_Identity=25, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 30012; Mature: 30012

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAVTGDGDADPDAPTGVELTRLRSAELFAMLRDDGAPAARRDAAREGLVHLHLPLVEHC
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCEEEEECHHHHHH
ARRFRNRGEPLEDLVQVGTIGLIKSVDRFDADRGVEFSTYATPTIIGEIKRYFRDKGWAI
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHCCCCEE
RVPRRLQELRMQISTASAELTQSLGRSPTPRELAGAIGCTVEEIVEGIESSNAYSTLSLD
ECHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEC
ATDDGDDNTASMLDAIGVDDEGLEHVEIRESLKPLLDRLEPREKKILLLRFFKNMTQSQI
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AEEIGVSQMHVSRLLNRTLEQLRVSLEETS
HHHHCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFAVTGDGDADPDAPTGVELTRLRSAELFAMLRDDGAPAARRDAAREGLVHLHLPLVEHC
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCEEEEECHHHHHH
ARRFRNRGEPLEDLVQVGTIGLIKSVDRFDADRGVEFSTYATPTIIGEIKRYFRDKGWAI
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHCCCCEE
RVPRRLQELRMQISTASAELTQSLGRSPTPRELAGAIGCTVEEIVEGIESSNAYSTLSLD
ECHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEC
ATDDGDDNTASMLDAIGVDDEGLEHVEIRESLKPLLDRLEPREKKILLLRFFKNMTQSQI
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AEEIGVSQMHVSRLLNRTLEQLRVSLEETS
HHHHCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7476207; 12000953 [H]