Click here to switch to the map view.

The map label for this gene is gacS [H]

Identifier: 119715905

GI number: 119715905

Start: 1776309

End: 1778561

Strand: Reverse

Name: gacS [H]

Synonym: Noca_1670

Alternate gene names: 119715905

Gene position: 1778561-1776309 (Counterclockwise)

Preceding gene: 119715906

Following gene: 119715899

Centisome position: 35.67

GC content: 74.39

Gene sequence:

>2253_bases
ATGACCAGCCACGAGCGGATCCAGCCCGTCATCGAGCTGCTGACCAGGCTGGCGACCGGCGACCTCGATGCCCGCGGCGC
CCGGATCGCCGGCGACGAGGACCTGGACGCGGTGATCTTCGGCATCAACATGCTCGCCGAGGAGCTCAGCGCCAGCCGGA
CCGACCTCGAGAAGCGGATCGAGGCGCGCACCCACGACCTCGAGATCACGCGCCGGGAGGCGGTGCGCGCCCGCAAGGAG
GCGGAGGCCGCCACCGCCGCCAAGTCGGCGTTCCTGGCCACGATGAGCCACGAGATCCGGACCCCGATGAACGGGGTCAT
CGGCCTGACCCGGCTGCTGCTCGACACCGAGCTCGACGAGACCCAACGGCGCTACGCCAAGGGTCTGAGCGAGGCCGGGA
CCACGCTGCTGACGCTGATCGACGACATCCTCGACTTCTCCAAGATCGAGGCGGGGATGCTCGACCTCGAGCGGGCGCCG
TTCGATCCCCGGCGGACCCTGGACGCCGTTGCCGGCCTGGTGGCGAACAGCGCGGCTCGCAAGGACGTGGAGCTCGTCGT
CGAGGCGGTCCCGGACCTGCCCGCGGGCCTCGTCGGCGACGAGGCCCGCCTGCGCCAGGTGCTCCTGAACCTCGCCTCGA
ACGCGGTGAAGTTCACGTCCACCGGAGAGGTCGTGATCCGGGCGACCCTGGACCGGTCCGGCCAGCCGGCCGCGAGTCCG
GGCGAGACCCCCGTCCGGTTCGAGGTCGTCGACACCGGCATCGGCATCCCGCAGGCCGCGCAGGCGCGCCTGTTCCGGTC
GTTCACCCAGGCCGAGGCGTCCACCACGCGTAGGTACGGCGGCACCGGTCTCGGTCTCGCGATCTCGCGACGCCTGGTCC
GCCTGATGGGTGGGACCATCGGCTTCGAGAGCCAGGAGGGCCACGGCAGCACGTTCTGGTTCGCCGTACCGCTCACCGCC
GCGGACCTGGACACCAGCCGGGACGCGGACCCGGCCAGGGGGATCCCCGCGGACGCGCGCGCCCTGCTCGTCGACGACAA
CGCGACCCAGCGTCGCGTCCTCGGTGCCCAGCTGACCGAGGCGGGGCTCCGTACCGACGTGACCGGGCGCCCGGAGGCGG
TGCTGACGCTGATGCGGGCCGCCGCGGCGGCCGCGGACCCGTACGCCGTCGTCCTGGTCGACTCCCGGCTGCCGGGCGTC
GACGGCCTCGAGGTGCTGGCTGCGATCGGGGCCGACCCCGCGTTGCGGGACAGCCGCCCCGTCCTGCTGTCCGCGACGGC
CGAGCTCGACCACGACGCCGTGCTCCGCGCCGGCGTGAGCACGGTCGTGACCAAACCGGTCCGCCTGAGCGAGCTCCGGA
GCCGGCTGACCGAGCTGCTGGCGCCGCCCGCACCGACCGCTCCCGAGCCGGACGCGCCGGCCCCGCCCCGCGGGTCGGCG
CCGAGCCGCGGGTCGGCGCCGAGCCGCGGCCGGGTGCTGGTCGCCGAGGACAACTCGATCAACCAGCTGGTCGCCGAGGG
CCTGCTCACCAGGCTCGGCTTCGAGGTGGACCTCGCCGGCGACGGCAAGGAAGCGGTCGCGGCCGTCGCGCGCCAGTCCT
ACACCGCGGTGCTGATGGACGGCCAGATGCCGATCATGGACGGCTACCAGGCGACCGCGGAGATCCGCAGCCGTGAGGTC
GGGACCCGACTGCCGATCATCGCGATGACCGCGTCCGCCACCGAGGACGACAAGCGGCGCGCGCTCGCCGCCGGGATGGA
CGGGTTCGTGGCCAAGCCGGTCGACGCGGCCACCTTGGACCGGGTGCTCAGCCAGTGGGCTCCGGCCGAGCCGGTCGACC
AGGGCGCACCGTCCGACGAGGAGCCCGCACCGGCCGACGGGCATCCGACCGAGGACCTCGGTCCGGCCCTCGACCTGGAG
CGCGTCACGTTGCTGCGCGGTCTGGGGAACGCGAGCACCGGAGGGCTGCTGCCCACGCTGGTCGAGGCCTTCGCAGCGTC
CGCGCCGAGCCTCGCCCACACCATTGCAGAAGCCGCCGCCCGGAACGACTGGCCGGCGGTGCGCACCCGGGCCCACCAGC
TCGCCGGCGCCGCCGACAACATCGGCGCACTCGCGGCCGGCGGCCTGGCCCGGACCATCGAGCGGCACGCAGCCGAGGGC
CGGGAGGCCCCGGGCGAGGCGGCCGGCCGGATCGAGGCGGAGGTCGACCGCGCCGTCGACCAGCTGACCCGCACGCTGCA
GCGCGCCGGGTGA

Upstream 100 bases:

>100_bases
GGATGCTCGCGAACTTCTTCCTGGGCCTCAAGCGCGGCGAGAACCCGGTCCGGATGTTCGGCTCCGAGGCCGACGCGATC
GCCTGGCTCCAGGCGCAGCC

Downstream 100 bases:

>100_bases
CGGCGCGGCCCTCGCCTCGGCCCCGGGCTCAGCCGGCGCGCCGGGTCGCCTCGGTCCGCTCGACCAGGCGCGCGAACGCC
TGGGCGAAACCGTCGACCAC

Product: ATPase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 750; Mature: 749

Protein sequence:

>750_residues
MTSHERIQPVIELLTRLATGDLDARGARIAGDEDLDAVIFGINMLAEELSASRTDLEKRIEARTHDLEITRREAVRARKE
AEAATAAKSAFLATMSHEIRTPMNGVIGLTRLLLDTELDETQRRYAKGLSEAGTTLLTLIDDILDFSKIEAGMLDLERAP
FDPRRTLDAVAGLVANSAARKDVELVVEAVPDLPAGLVGDEARLRQVLLNLASNAVKFTSTGEVVIRATLDRSGQPAASP
GETPVRFEVVDTGIGIPQAAQARLFRSFTQAEASTTRRYGGTGLGLAISRRLVRLMGGTIGFESQEGHGSTFWFAVPLTA
ADLDTSRDADPARGIPADARALLVDDNATQRRVLGAQLTEAGLRTDVTGRPEAVLTLMRAAAAAADPYAVVLVDSRLPGV
DGLEVLAAIGADPALRDSRPVLLSATAELDHDAVLRAGVSTVVTKPVRLSELRSRLTELLAPPAPTAPEPDAPAPPRGSA
PSRGSAPSRGRVLVAEDNSINQLVAEGLLTRLGFEVDLAGDGKEAVAAVARQSYTAVLMDGQMPIMDGYQATAEIRSREV
GTRLPIIAMTASATEDDKRRALAAGMDGFVAKPVDAATLDRVLSQWAPAEPVDQGAPSDEEPAPADGHPTEDLGPALDLE
RVTLLRGLGNASTGGLLPTLVEAFAASAPSLAHTIAEAAARNDWPAVRTRAHQLAGAADNIGALAAGGLARTIERHAAEG
REAPGEAAGRIEAEVDRAVDQLTRTLQRAG

Sequences:

>Translated_750_residues
MTSHERIQPVIELLTRLATGDLDARGARIAGDEDLDAVIFGINMLAEELSASRTDLEKRIEARTHDLEITRREAVRARKE
AEAATAAKSAFLATMSHEIRTPMNGVIGLTRLLLDTELDETQRRYAKGLSEAGTTLLTLIDDILDFSKIEAGMLDLERAP
FDPRRTLDAVAGLVANSAARKDVELVVEAVPDLPAGLVGDEARLRQVLLNLASNAVKFTSTGEVVIRATLDRSGQPAASP
GETPVRFEVVDTGIGIPQAAQARLFRSFTQAEASTTRRYGGTGLGLAISRRLVRLMGGTIGFESQEGHGSTFWFAVPLTA
ADLDTSRDADPARGIPADARALLVDDNATQRRVLGAQLTEAGLRTDVTGRPEAVLTLMRAAAAAADPYAVVLVDSRLPGV
DGLEVLAAIGADPALRDSRPVLLSATAELDHDAVLRAGVSTVVTKPVRLSELRSRLTELLAPPAPTAPEPDAPAPPRGSA
PSRGSAPSRGRVLVAEDNSINQLVAEGLLTRLGFEVDLAGDGKEAVAAVARQSYTAVLMDGQMPIMDGYQATAEIRSREV
GTRLPIIAMTASATEDDKRRALAAGMDGFVAKPVDAATLDRVLSQWAPAEPVDQGAPSDEEPAPADGHPTEDLGPALDLE
RVTLLRGLGNASTGGLLPTLVEAFAASAPSLAHTIAEAAARNDWPAVRTRAHQLAGAADNIGALAAGGLARTIERHAAEG
REAPGEAAGRIEAEVDRAVDQLTRTLQRAG
>Mature_749_residues
TSHERIQPVIELLTRLATGDLDARGARIAGDEDLDAVIFGINMLAEELSASRTDLEKRIEARTHDLEITRREAVRARKEA
EAATAAKSAFLATMSHEIRTPMNGVIGLTRLLLDTELDETQRRYAKGLSEAGTTLLTLIDDILDFSKIEAGMLDLERAPF
DPRRTLDAVAGLVANSAARKDVELVVEAVPDLPAGLVGDEARLRQVLLNLASNAVKFTSTGEVVIRATLDRSGQPAASPG
ETPVRFEVVDTGIGIPQAAQARLFRSFTQAEASTTRRYGGTGLGLAISRRLVRLMGGTIGFESQEGHGSTFWFAVPLTAA
DLDTSRDADPARGIPADARALLVDDNATQRRVLGAQLTEAGLRTDVTGRPEAVLTLMRAAAAAADPYAVVLVDSRLPGVD
GLEVLAAIGADPALRDSRPVLLSATAELDHDAVLRAGVSTVVTKPVRLSELRSRLTELLAPPAPTAPEPDAPAPPRGSAP
SRGSAPSRGRVLVAEDNSINQLVAEGLLTRLGFEVDLAGDGKEAVAAVARQSYTAVLMDGQMPIMDGYQATAEIRSREVG
TRLPIIAMTASATEDDKRRALAAGMDGFVAKPVDAATLDRVLSQWAPAEPVDQGAPSDEEPAPADGHPTEDLGPALDLER
VTLLRGLGNASTGGLLPTLVEAFAASAPSLAHTIAEAAARNDWPAVRTRAHQLAGAADNIGALAAGGLARTIERHAAEGR
EAPGEAAGRIEAEVDRAVDQLTRTLQRAG

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=614, Percent_Identity=33.8762214983713, Blast_Score=304, Evalue=1e-83,
Organism=Escherichia coli, GI145693157, Length=534, Percent_Identity=33.1460674157303, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI87081816, Length=446, Percent_Identity=34.5291479820628, Blast_Score=205, Evalue=1e-53,
Organism=Escherichia coli, GI48994928, Length=384, Percent_Identity=35.4166666666667, Blast_Score=200, Evalue=3e-52,
Organism=Escherichia coli, GI1788713, Length=421, Percent_Identity=30.8788598574822, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788393, Length=219, Percent_Identity=30.1369863013699, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788549, Length=254, Percent_Identity=26.7716535433071, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1786600, Length=265, Percent_Identity=27.9245283018868, Blast_Score=74, Evalue=5e-14,
Organism=Escherichia coli, GI87082128, Length=239, Percent_Identity=28.0334728033473, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1790436, Length=241, Percent_Identity=29.8755186721992, Blast_Score=73, Evalue=8e-14,
Organism=Escherichia coli, GI1790300, Length=312, Percent_Identity=27.5641025641026, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1786912, Length=236, Percent_Identity=28.8135593220339, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1786783, Length=272, Percent_Identity=29.4117647058824, Blast_Score=70, Evalue=6e-13,
Organism=Escherichia coli, GI1788191, Length=116, Percent_Identity=35.3448275862069, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=136, Percent_Identity=36.0294117647059, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323034, Length=172, Percent_Identity=29.6511627906977, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 78918; Mature: 78787

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50894 HPT ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSHERIQPVIELLTRLATGDLDARGARIAGDEDLDAVIFGINMLAEELSASRTDLEKRI
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EARTHDLEITRREAVRARKEAEAATAAKSAFLATMSHEIRTPMNGVIGLTRLLLDTELDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHH
TQRRYAKGLSEAGTTLLTLIDDILDFSKIEAGMLDLERAPFDPRRTLDAVAGLVANSAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KDVELVVEAVPDLPAGLVGDEARLRQVLLNLASNAVKFTSTGEVVIRATLDRSGQPAASP
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCEEEEEECCCCCCCCCCC
GETPVRFEVVDTGIGIPQAAQARLFRSFTQAEASTTRRYGGTGLGLAISRRLVRLMGGTI
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
GFESQEGHGSTFWFAVPLTAADLDTSRDADPARGIPADARALLVDDNATQRRVLGAQLTE
CCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
AGLRTDVTGRPEAVLTLMRAAAAAADPYAVVLVDSRLPGVDGLEVLAAIGADPALRDSRP
HCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCC
VLLSATAELDHDAVLRAGVSTVVTKPVRLSELRSRLTELLAPPAPTAPEPDAPAPPRGSA
EEEEECCCCCHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PSRGSAPSRGRVLVAEDNSINQLVAEGLLTRLGFEVDLAGDGKEAVAAVARQSYTAVLMD
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHCCEEEEEC
GQMPIMDGYQATAEIRSREVGTRLPIIAMTASATEDDKRRALAAGMDGFVAKPVDAATLD
CCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHH
RVLSQWAPAEPVDQGAPSDEEPAPADGHPTEDLGPALDLERVTLLRGLGNASTGGLLPTL
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHCCCCCCCCHHHHH
VEAFAASAPSLAHTIAEAAARNDWPAVRTRAHQLAGAADNIGALAAGGLARTIERHAAEG
HHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
REAPGEAAGRIEAEVDRAVDQLTRTLQRAG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSHERIQPVIELLTRLATGDLDARGARIAGDEDLDAVIFGINMLAEELSASRTDLEKRI
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EARTHDLEITRREAVRARKEAEAATAAKSAFLATMSHEIRTPMNGVIGLTRLLLDTELDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHH
TQRRYAKGLSEAGTTLLTLIDDILDFSKIEAGMLDLERAPFDPRRTLDAVAGLVANSAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KDVELVVEAVPDLPAGLVGDEARLRQVLLNLASNAVKFTSTGEVVIRATLDRSGQPAASP
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCEEEEEECCCCCCCCCCC
GETPVRFEVVDTGIGIPQAAQARLFRSFTQAEASTTRRYGGTGLGLAISRRLVRLMGGTI
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
GFESQEGHGSTFWFAVPLTAADLDTSRDADPARGIPADARALLVDDNATQRRVLGAQLTE
CCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
AGLRTDVTGRPEAVLTLMRAAAAAADPYAVVLVDSRLPGVDGLEVLAAIGADPALRDSRP
HCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCC
VLLSATAELDHDAVLRAGVSTVVTKPVRLSELRSRLTELLAPPAPTAPEPDAPAPPRGSA
EEEEECCCCCHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PSRGSAPSRGRVLVAEDNSINQLVAEGLLTRLGFEVDLAGDGKEAVAAVARQSYTAVLMD
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHCCEEEEEC
GQMPIMDGYQATAEIRSREVGTRLPIIAMTASATEDDKRRALAAGMDGFVAKPVDAATLD
CCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHH
RVLSQWAPAEPVDQGAPSDEEPAPADGHPTEDLGPALDLERVTLLRGLGNASTGGLLPTL
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHCCCCCCCCHHHHH
VEAFAASAPSLAHTIAEAAARNDWPAVRTRAHQLAGAADNIGALAAGGLARTIERHAAEG
HHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
REAPGEAAGRIEAEVDRAVDQLTRTLQRAG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]