Click here to switch to the map view.

The map label for this gene is metG

Identifier: 119715622

GI number: 119715622

Start: 1462407

End: 1464194

Strand: Direct

Name: metG

Synonym: Noca_1386

Alternate gene names: 119715622

Gene position: 1462407-1464194 (Clockwise)

Preceding gene: 119715620

Following gene: 119715625

Centisome position: 29.33

GC content: 69.07

Gene sequence:

>1788_bases
ATGAGTCGCGTCCTGTCCGCCGTCGCCTGGCCGTACGCCAACGGCCCGCGCCACATCGGCCACGTCGCCGGTTTCGGCGT
GCCCTCCGACGTCTTCAGCCGGTACATGCGGATGGCGGGCCACGACGTGCTCATGGTCTCCGGCTCCGACGAGCACGGGA
CACCGATCCTGATCGCGGCCGACGAGGCCGGGCTCTCGCCGCAGGAGCTGGCCGATCGCAACCACCGGATCATCGCCGAG
GACCTGGCCTCGCTCGGCCTGACCTACGACCTCTACACGCGCACCACCACCCGCAACCACCACGCGGTCGTGCAGGAGCT
GTTCCTCGGCGTCTACGAGAACGGGTACTTCGTCGAGCAGACGACGTACGGCGCCATCTCGCCCTCGACCGGGCGCACCC
TGCCCGACCGCTACATCGAGGGCACCTGCCCGATCTGCGGGTACGACGGCGCGCGCGGCGACCAGTGCGACAACTGCGGC
AACCAGCTGGACCCGCACGACCTGATCGACCCGCGCTCGAAGATCAACGGCGAGACGCCGGAGTTCATCGAGACCCAGCA
CTTCTTCCTCGACCTGCCCGCGCTGGCCGAGGCGCTGGGGGAGTGGCTCGACGGGCGCGAGGCGACGGGGCTCTGGCGGC
CGAACGTGATCCGGTTCAGCCAGAACATCCTCAAGGAGATCCGGCCGCGAGCGATGACCCGCGACATCGACTGGGGCATC
GCCGTACCCCTCGACGGCTGGCGGGAGAACCCGACCAAGAGGCTCTACGTCTGGTTCGACGCGGTGATCGGCTACCTGTC
GGCGTCCATCGAGTGGGCCCGCCGTCTCGACGAGCCCGACCGCTGGCGCGACTGGTGGAACGACCCGGACGCCCTGTCGT
ACTACTTCATGGGCAAGGACAACATCACCTTCCACAGCCAGATCTGGCCGGCCGAGCTGCTGGCCTACTCCGGCAAGGGC
GCGAAGGGCGGCACGCCGCGACAGTACGGCGAGCTGAACCTGCCCACCGAGGTCGTCTCCTCGGAGTTCCTCACGATGGA
GGGGCGCAAGTTCTCCTCCTCCAAACGGGTCGTGATCTACGTGCGCGACCTGCTCAGCCGGTACCAGCCGGACGCGTTCC
GCTACTTCGTCGCGGCCGCCGGGCCGGAGAACCAGGACTCCGATTTCACCTGGTCGGAGTTCGTGCGTCGCACCAACGAC
GAGCTGGTCGCCGGCTGGGGCAACCTGGTCAACCGCACCGCGAACCTGATCGCGAAGAACTTCGGGGAGATCCCGAAGGC
CGGCGAGCTCACCGCAGCGGACCAGGCTCTCCTGGACTCCGTGGCCTCGGCGTTCGGCGTCGTCGGCGACCTCATCGGCC
GGCACCGGCAGAAGCAGGCGATCGGCGAGGCGATGCGGGCCGTGGCCGAGGTCAACAAGTACGTCTCCGACACCGAGCCG
TGGAAGATCAAGGACGACCCCGACCGGCTCGGCACGATCCTGCACGTCGTCGCCCAGTGTGTGGCCGACCTCAACCTGAT
CCTCTCGCCGTTCCTGCCGTTCGCCGCGAACGAGGTGGACCGGGCGCTGGGCGGCTCGGGCGAGATCGCCCCGATGCCGC
GCCTGGAGGAGGTCGACGACCTCGACGGCGGCGCGCCGTACCCGATCATCACCGGGGAGTACTCCGGCTTCCCCGCCTGG
GAGCGGCGCCCGATCGTGCCCGGCACCCCGGTCTCGAAGCCCACGCCGATCTTCACCAAGCTCGACCCGTCGATCGTCGA
CGAGGAGCTCGCCCGGCTGGAGAGCTGA

Upstream 100 bases:

>100_bases
AGCAACAGGAGCAGGTCCTCCGCGATCAGGGTCTCCACGATCTGACGGTAGCGCGACGCCGCGGGAAACCCGCTGGCCGG
CGATCCCTAGGATTGACCCC

Downstream 100 bases:

>100_bases
GGGGCGACACGCCGGCGGGGTTCGGCTGCCAGGTGACGCCTCGGACCGGTCGCGTGCCTCAGTCGTCGCGCACCCGACCG
CGCAGCGACTTCGTCCGGCC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 595; Mature: 594

Protein sequence:

>595_residues
MSRVLSAVAWPYANGPRHIGHVAGFGVPSDVFSRYMRMAGHDVLMVSGSDEHGTPILIAADEAGLSPQELADRNHRIIAE
DLASLGLTYDLYTRTTTRNHHAVVQELFLGVYENGYFVEQTTYGAISPSTGRTLPDRYIEGTCPICGYDGARGDQCDNCG
NQLDPHDLIDPRSKINGETPEFIETQHFFLDLPALAEALGEWLDGREATGLWRPNVIRFSQNILKEIRPRAMTRDIDWGI
AVPLDGWRENPTKRLYVWFDAVIGYLSASIEWARRLDEPDRWRDWWNDPDALSYYFMGKDNITFHSQIWPAELLAYSGKG
AKGGTPRQYGELNLPTEVVSSEFLTMEGRKFSSSKRVVIYVRDLLSRYQPDAFRYFVAAAGPENQDSDFTWSEFVRRTND
ELVAGWGNLVNRTANLIAKNFGEIPKAGELTAADQALLDSVASAFGVVGDLIGRHRQKQAIGEAMRAVAEVNKYVSDTEP
WKIKDDPDRLGTILHVVAQCVADLNLILSPFLPFAANEVDRALGGSGEIAPMPRLEEVDDLDGGAPYPIITGEYSGFPAW
ERRPIVPGTPVSKPTPIFTKLDPSIVDEELARLES

Sequences:

>Translated_595_residues
MSRVLSAVAWPYANGPRHIGHVAGFGVPSDVFSRYMRMAGHDVLMVSGSDEHGTPILIAADEAGLSPQELADRNHRIIAE
DLASLGLTYDLYTRTTTRNHHAVVQELFLGVYENGYFVEQTTYGAISPSTGRTLPDRYIEGTCPICGYDGARGDQCDNCG
NQLDPHDLIDPRSKINGETPEFIETQHFFLDLPALAEALGEWLDGREATGLWRPNVIRFSQNILKEIRPRAMTRDIDWGI
AVPLDGWRENPTKRLYVWFDAVIGYLSASIEWARRLDEPDRWRDWWNDPDALSYYFMGKDNITFHSQIWPAELLAYSGKG
AKGGTPRQYGELNLPTEVVSSEFLTMEGRKFSSSKRVVIYVRDLLSRYQPDAFRYFVAAAGPENQDSDFTWSEFVRRTND
ELVAGWGNLVNRTANLIAKNFGEIPKAGELTAADQALLDSVASAFGVVGDLIGRHRQKQAIGEAMRAVAEVNKYVSDTEP
WKIKDDPDRLGTILHVVAQCVADLNLILSPFLPFAANEVDRALGGSGEIAPMPRLEEVDDLDGGAPYPIITGEYSGFPAW
ERRPIVPGTPVSKPTPIFTKLDPSIVDEELARLES
>Mature_594_residues
SRVLSAVAWPYANGPRHIGHVAGFGVPSDVFSRYMRMAGHDVLMVSGSDEHGTPILIAADEAGLSPQELADRNHRIIAED
LASLGLTYDLYTRTTTRNHHAVVQELFLGVYENGYFVEQTTYGAISPSTGRTLPDRYIEGTCPICGYDGARGDQCDNCGN
QLDPHDLIDPRSKINGETPEFIETQHFFLDLPALAEALGEWLDGREATGLWRPNVIRFSQNILKEIRPRAMTRDIDWGIA
VPLDGWRENPTKRLYVWFDAVIGYLSASIEWARRLDEPDRWRDWWNDPDALSYYFMGKDNITFHSQIWPAELLAYSGKGA
KGGTPRQYGELNLPTEVVSSEFLTMEGRKFSSSKRVVIYVRDLLSRYQPDAFRYFVAAAGPENQDSDFTWSEFVRRTNDE
LVAGWGNLVNRTANLIAKNFGEIPKAGELTAADQALLDSVASAFGVVGDLIGRHRQKQAIGEAMRAVAEVNKYVSDTEPW
KIKDDPDRLGTILHVVAQCVADLNLILSPFLPFAANEVDRALGGSGEIAPMPRLEEVDDLDGGAPYPIITGEYSGFPAWE
RRPIVPGTPVSKPTPIFTKLDPSIVDEELARLES

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=528, Percent_Identity=34.469696969697, Blast_Score=323, Evalue=4e-88,
Organism=Homo sapiens, GI24308436, Length=556, Percent_Identity=25.8992805755396, Blast_Score=157, Evalue=3e-38,
Organism=Escherichia coli, GI1788432, Length=525, Percent_Identity=28.952380952381, Blast_Score=245, Evalue=7e-66,
Organism=Caenorhabditis elegans, GI17540794, Length=599, Percent_Identity=30.7178631051753, Blast_Score=307, Evalue=8e-84,
Organism=Caenorhabditis elegans, GI212645096, Length=540, Percent_Identity=25.5555555555556, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71992781, Length=371, Percent_Identity=25.0673854447439, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321704, Length=598, Percent_Identity=31.2709030100334, Blast_Score=318, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24655311, Length=603, Percent_Identity=32.1724709784411, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI21358483, Length=531, Percent_Identity=24.4821092278719, Blast_Score=106, Evalue=4e-23,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_NOCSJ (A1SGG5)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_922587.1
- STRING:   A1SGG5
- GeneID:   4595859
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_1386
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   CGNQLDP
- PhylomeDB:   A1SGG5
- ProtClustDB:   PRK12268
- BioCyc:   NSP35761:NOCA_1386-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 66252; Mature: 66121

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 353-353

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRVLSAVAWPYANGPRHIGHVAGFGVPSDVFSRYMRMAGHDVLMVSGSDEHGTPILIAA
CCHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEC
DEAGLSPQELADRNHRIIAEDLASLGLTYDLYTRTTTRNHHAVVQELFLGVYENGYFVEQ
CCCCCCHHHHHCCCCEEHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEE
TTYGAISPSTGRTLPDRYIEGTCPICGYDGARGDQCDNCGNQLDPHDLIDPRSKINGETP
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCHHCCCCHHHCCCCCC
EFIETQHFFLDLPALAEALGEWLDGREATGLWRPNVIRFSQNILKEIRPRAMTRDIDWGI
HHHHHHHHEEECHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCHHHHCCCCCCCE
AVPLDGWRENPTKRLYVWFDAVIGYLSASIEWARRLDEPDRWRDWWNDPDALSYYFMGKD
ECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCC
NITFHSQIWPAELLAYSGKGAKGGTPRQYGELNLPTEVVSSEFLTMEGRKFSSSKRVVIY
CCEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCCCCCCCCEEEEE
VRDLLSRYQPDAFRYFVAAAGPENQDSDFTWSEFVRRTNDELVAGWGNLVNRTANLIAKN
HHHHHHHHCCCHHEEEEEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
FGEIPKAGELTAADQALLDSVASAFGVVGDLIGRHRQKQAIGEAMRAVAEVNKYVSDTEP
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WKIKDDPDRLGTILHVVAQCVADLNLILSPFLPFAANEVDRALGGSGEIAPMPRLEEVDD
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHCC
LDGGAPYPIITGEYSGFPAWERRPIVPGTPVSKPTPIFTKLDPSIVDEELARLES
CCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SRVLSAVAWPYANGPRHIGHVAGFGVPSDVFSRYMRMAGHDVLMVSGSDEHGTPILIAA
CHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEC
DEAGLSPQELADRNHRIIAEDLASLGLTYDLYTRTTTRNHHAVVQELFLGVYENGYFVEQ
CCCCCCHHHHHCCCCEEHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEE
TTYGAISPSTGRTLPDRYIEGTCPICGYDGARGDQCDNCGNQLDPHDLIDPRSKINGETP
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCHHCCCCHHHCCCCCC
EFIETQHFFLDLPALAEALGEWLDGREATGLWRPNVIRFSQNILKEIRPRAMTRDIDWGI
HHHHHHHHEEECHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCHHHHCCCCCCCE
AVPLDGWRENPTKRLYVWFDAVIGYLSASIEWARRLDEPDRWRDWWNDPDALSYYFMGKD
ECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCC
NITFHSQIWPAELLAYSGKGAKGGTPRQYGELNLPTEVVSSEFLTMEGRKFSSSKRVVIY
CCEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCCCCCCCCEEEEE
VRDLLSRYQPDAFRYFVAAAGPENQDSDFTWSEFVRRTNDELVAGWGNLVNRTANLIAKN
HHHHHHHHCCCHHEEEEEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
FGEIPKAGELTAADQALLDSVASAFGVVGDLIGRHRQKQAIGEAMRAVAEVNKYVSDTEP
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WKIKDDPDRLGTILHVVAQCVADLNLILSPFLPFAANEVDRALGGSGEIAPMPRLEEVDD
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHCC
LDGGAPYPIITGEYSGFPAWERRPIVPGTPVSKPTPIFTKLDPSIVDEELARLES
CCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA