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The map label for this gene is narH [H]

Identifier: 119715583

GI number: 119715583

Start: 1424993

End: 1426747

Strand: Direct

Name: narH [H]

Synonym: Noca_1347

Alternate gene names: 119715583

Gene position: 1424993-1426747 (Clockwise)

Preceding gene: 119715582

Following gene: 119715584

Centisome position: 28.58

GC content: 68.43

Gene sequence:

>1755_bases
ATGAAGGTAATGGCCCAGATGGCCATGGTGATGAACCTCGACAAGTGCATCGGGTGCCACACCTGCTCCGTGACCTGCAA
GCAGGCGTGGACCAACCGGACCGGCACCGAGTACGTCTGGTTCAACAACGTCGAGACGCGGCCCGGCCAGGGCTACCCGC
GGACCTACGAGGACCAGGAGGAGTGGAAGGGCGGCTGGGAGCTCAACAGCCGCGGCCGGCTCAAGCTCAAGGCCGGCGGC
CGGTTGAAGAAGCTCGCGACGATCTTCTCCAACCCGAAGCTGCCCTCGATCCAGGACTACTACGAGCCGTGGACCTACGA
CTACGCCACGCTCACGGACGCGCCCGCACAGAAGCACACGCCGGTTGCGCGCCCGAAGTCCCTGCTGACCGGCAAGGACA
TCAAGATCGAGTGGTCCTCGAACTGGGACGACGACCTCGGCGGCTCGGCGGCCACGGCGAAGCACGACCCGATGCTCAAG
AAGATCGCCGACAAGGTGAAGTTCGAGTTCGAGCAGACCTTCATGTTCTACCTGCCGCGCATCTGCGAGCACTGCCTGAA
CCCGTCCTGTGCGGCGTCCTGCCCCAGCGGCGCGATCTACAAGCGCGCCGAGGACGGCATCGTGCTGGTCGACCAGGACC
GGTGCCGAGGCTGGCGGATGTGCGTCTCGGGCTGCCCGTACAAGAAGGTCTACTTCAACCACCGCACCGGCAAGGCCGAG
AAGTGCACGTTCTGCTTCCCGCGCATCGAGGTCGGACTGCCGACCGTGTGCGCGGAGACGTGTGTCGGCCGGCTGCGTTA
CATCGGCCTGGTGCTCTACGACGCCGACCGGGTGCTCGAGGCGGCCTCGGTGCCCGACGAGCACGACCTCTACGAGGCCC
AGCGCTCGATCCTGCTCGACCCCGACGACCCCGAGGTGGTCCGGGCGGCCGAGGCAGCCGGCATCCCGGCGGACTGGATC
GACGCCGCGCAGCGCTCGCCGATCCACGCGCTGATCAACAAGTACAAGGTCGCGCTGCCGCTGCACCCGGAGTACCGCAC
CATGCCGATGGTCTGGTACATCCCGCCGCTCTCGCCGGTCGTGGACGTGGTCCGCGACACCGGCGAGGACGCCGAGGACA
CCGGCAACCTGTTCGCGGCGATCGACGCGCTGCGGATCCCCGTCGAGTACCTCGCCGAGCTGTTCACGGCCGGGGACGTG
GCTCCCGTGGACCGGGTCCTCAAGCAGCTGGCGGCGATGCGCTCCTACATGCGCGACATCAACATGGGTCGCGAGTCGAA
CCCCGCCATCCCCGCGGCGGTCGGCATGACCGAGGAGCAGATGTACGACATGTACCGCCTCCTCGCGATCGCCAAGTACG
ACGAGCGGTACGTGATCCCGGCCGCACACTCCGAGCAGGCGCACTCACTGGAGGAGCTCGGGACCGAGTGCGCGGTCGCG
GACTACGGCGGTGGCCAGCAGGACCTGTTCGGAGAGGGCTCCGGGGTGCCGACGCCGATCGCGGTGGAGAACTTCCAGAT
GCTGCAGAACCGGCAGACGGCGGACACCTTCGCCAGCCCCGACGACAAGGGCTCCCGGGTGAACCTCCTCAACTGGGACG
GTCGCGGCAAGCCCAGCGGGCTGTTCCCGCCGCGACCGGACGGGCCTGGCGGGCCGGCGGACACGGCCGAGGGCGGCACC
CCCGACACCAGCCACGACGGCGAGACCGGTCCGGGTGGCACGGGCGGACTGACCGGAGGGGCGGACTTCCGATGA

Upstream 100 bases:

>100_bases
CGCAGGACGTGGAGTACTGAATGGCACCCCACGAAGTTCTCCGCTCGTTCCTTGCTCCGAAACTCCGCGGGGGCCCCGAC
GCCTCGGAAGGTTGTGACCG

Downstream 100 bases:

>100_bases
GCCGTCGCTCCGCTCGGCCCGAGCTGTCACCTGGCCAGCTCACGATCGCCTGGCAGTCGGCCTCCCTGCTGCTCGGCTAT
CCCGACCAGACGCTGCTCGA

Product: respiratory nitrate reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MKVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRTGTEYVWFNNVETRPGQGYPRTYEDQEEWKGGWELNSRGRLKLKAGG
RLKKLATIFSNPKLPSIQDYYEPWTYDYATLTDAPAQKHTPVARPKSLLTGKDIKIEWSSNWDDDLGGSAATAKHDPMLK
KIADKVKFEFEQTFMFYLPRICEHCLNPSCAASCPSGAIYKRAEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHRTGKAE
KCTFCFPRIEVGLPTVCAETCVGRLRYIGLVLYDADRVLEAASVPDEHDLYEAQRSILLDPDDPEVVRAAEAAGIPADWI
DAAQRSPIHALINKYKVALPLHPEYRTMPMVWYIPPLSPVVDVVRDTGEDAEDTGNLFAAIDALRIPVEYLAELFTAGDV
APVDRVLKQLAAMRSYMRDINMGRESNPAIPAAVGMTEEQMYDMYRLLAIAKYDERYVIPAAHSEQAHSLEELGTECAVA
DYGGGQQDLFGEGSGVPTPIAVENFQMLQNRQTADTFASPDDKGSRVNLLNWDGRGKPSGLFPPRPDGPGGPADTAEGGT
PDTSHDGETGPGGTGGLTGGADFR

Sequences:

>Translated_584_residues
MKVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRTGTEYVWFNNVETRPGQGYPRTYEDQEEWKGGWELNSRGRLKLKAGG
RLKKLATIFSNPKLPSIQDYYEPWTYDYATLTDAPAQKHTPVARPKSLLTGKDIKIEWSSNWDDDLGGSAATAKHDPMLK
KIADKVKFEFEQTFMFYLPRICEHCLNPSCAASCPSGAIYKRAEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHRTGKAE
KCTFCFPRIEVGLPTVCAETCVGRLRYIGLVLYDADRVLEAASVPDEHDLYEAQRSILLDPDDPEVVRAAEAAGIPADWI
DAAQRSPIHALINKYKVALPLHPEYRTMPMVWYIPPLSPVVDVVRDTGEDAEDTGNLFAAIDALRIPVEYLAELFTAGDV
APVDRVLKQLAAMRSYMRDINMGRESNPAIPAAVGMTEEQMYDMYRLLAIAKYDERYVIPAAHSEQAHSLEELGTECAVA
DYGGGQQDLFGEGSGVPTPIAVENFQMLQNRQTADTFASPDDKGSRVNLLNWDGRGKPSGLFPPRPDGPGGPADTAEGGT
PDTSHDGETGPGGTGGLTGGADFR
>Mature_584_residues
MKVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRTGTEYVWFNNVETRPGQGYPRTYEDQEEWKGGWELNSRGRLKLKAGG
RLKKLATIFSNPKLPSIQDYYEPWTYDYATLTDAPAQKHTPVARPKSLLTGKDIKIEWSSNWDDDLGGSAATAKHDPMLK
KIADKVKFEFEQTFMFYLPRICEHCLNPSCAASCPSGAIYKRAEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHRTGKAE
KCTFCFPRIEVGLPTVCAETCVGRLRYIGLVLYDADRVLEAASVPDEHDLYEAQRSILLDPDDPEVVRAAEAAGIPADWI
DAAQRSPIHALINKYKVALPLHPEYRTMPMVWYIPPLSPVVDVVRDTGEDAEDTGNLFAAIDALRIPVEYLAELFTAGDV
APVDRVLKQLAAMRSYMRDINMGRESNPAIPAAVGMTEEQMYDMYRLLAIAKYDERYVIPAAHSEQAHSLEELGTECAVA
DYGGGQQDLFGEGSGVPTPIAVENFQMLQNRQTADTFASPDDKGSRVNLLNWDGRGKPSGLFPPRPDGPGGPADTAEGGT
PDTSHDGETGPGGTGGLTGGADFR

Specific function: This Is A Second Nitrate Reductase Enzyme Which Can Substitute For The Nra Enzyme And Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=468, Percent_Identity=56.1965811965812, Blast_Score=576, Evalue=1e-165,
Organism=Escherichia coli, GI1787478, Length=470, Percent_Identity=55.531914893617, Blast_Score=566, Evalue=1e-163,
Organism=Escherichia coli, GI1787122, Length=91, Percent_Identity=42.8571428571429, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787872, Length=101, Percent_Identity=40.5940594059406, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI226510944, Length=91, Percent_Identity=37.3626373626374, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1789370, Length=102, Percent_Identity=34.3137254901961, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1787749, Length=80, Percent_Identity=35, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI2367345, Length=94, Percent_Identity=34.0425531914894, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1790326, Length=81, Percent_Identity=38.2716049382716, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 64491; Mature: 64491

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRTGTEYVWFNNVETRPGQGYPRTYEDQE
CHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHH
EWKGGWELNSRGRLKLKAGGRLKKLATIFSNPKLPSIQDYYEPWTYDYATLTDAPAQKHT
HHCCCCEECCCCEEEEECCCCHHHHHHHHCCCCCCCHHHHHCCCCCCCCEECCCCCCCCC
PVARPKSLLTGKDIKIEWSSNWDDDLGGSAATAKHDPMLKKIADKVKFEFEQTFMFYLPR
CCCCCHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ICEHCLNPSCAASCPSGAIYKRAEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHRTGKAE
HHHHHCCCCHHCCCCCCCCEEECCCCEEEEECHHCCHHHHHHCCCCCEEEEEECCCCCCC
KCTFCFPRIEVGLPTVCAETCVGRLRYIGLVLYDADRVLEAASVPDEHDLYEAQRSILLD
EEEEECCCHHCCCCHHHHHHHHHHHHHHHEEEECHHHHHHHHCCCCHHHHHHHHHCEECC
PDDPEVVRAAEAAGIPADWIDAAQRSPIHALINKYKVALPLHPEYRTMPMVWYIPPLSPV
CCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHCEEEEEECCCCCCCCCEEEEECCHHHH
VDVVRDTGEDAEDTGNLFAAIDALRIPVEYLAELFTAGDVAPVDRVLKQLAAMRSYMRDI
HHHHHHCCCCCHHHCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
NMGRESNPAIPAAVGMTEEQMYDMYRLLAIAKYDERYVIPAAHSEQAHSLEELGTECAVA
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCEEEE
DYGGGQQDLFGEGSGVPTPIAVENFQMLQNRQTADTFASPDDKGSRVNLLNWDGRGKPSG
CCCCCCHHHCCCCCCCCCCEEHHHHHHHHCCCCHHHCCCCCCCCCEEEEEECCCCCCCCC
LFPPRPDGPGGPADTAEGGTPDTSHDGETGPGGTGGLTGGADFR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRTGTEYVWFNNVETRPGQGYPRTYEDQE
CHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHH
EWKGGWELNSRGRLKLKAGGRLKKLATIFSNPKLPSIQDYYEPWTYDYATLTDAPAQKHT
HHCCCCEECCCCEEEEECCCCHHHHHHHHCCCCCCCHHHHHCCCCCCCCEECCCCCCCCC
PVARPKSLLTGKDIKIEWSSNWDDDLGGSAATAKHDPMLKKIADKVKFEFEQTFMFYLPR
CCCCCHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ICEHCLNPSCAASCPSGAIYKRAEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHRTGKAE
HHHHHCCCCHHCCCCCCCCEEECCCCEEEEECHHCCHHHHHHCCCCCEEEEEECCCCCCC
KCTFCFPRIEVGLPTVCAETCVGRLRYIGLVLYDADRVLEAASVPDEHDLYEAQRSILLD
EEEEECCCHHCCCCHHHHHHHHHHHHHHHEEEECHHHHHHHHCCCCHHHHHHHHHCEECC
PDDPEVVRAAEAAGIPADWIDAAQRSPIHALINKYKVALPLHPEYRTMPMVWYIPPLSPV
CCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHCEEEEEECCCCCCCCCEEEEECCHHHH
VDVVRDTGEDAEDTGNLFAAIDALRIPVEYLAELFTAGDVAPVDRVLKQLAAMRSYMRDI
HHHHHHCCCCCHHHCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
NMGRESNPAIPAAVGMTEEQMYDMYRLLAIAKYDERYVIPAAHSEQAHSLEELGTECAVA
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCEEEE
DYGGGQQDLFGEGSGVPTPIAVENFQMLQNRQTADTFASPDDKGSRVNLLNWDGRGKPSG
CCCCCCHHHCCCCCCCCCCEEHHHHHHHHCCCCHHHCCCCCCCCCEEEEEECCCCCCCCC
LFPPRPDGPGGPADTAEGGTPDTSHDGETGPGGTGGLTGGADFR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]