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The map label for this gene is narK2 [H]

Identifier: 119715581

GI number: 119715581

Start: 1419949

End: 1421193

Strand: Direct

Name: narK2 [H]

Synonym: Noca_1345

Alternate gene names: 119715581

Gene position: 1419949-1421193 (Clockwise)

Preceding gene: 119715579

Following gene: 119715582

Centisome position: 28.48

GC content: 70.92

Gene sequence:

>1245_bases
ATGACCGTGGCGGAGGAGGGCATCGTGGGCAGTCAGCGCGGCCTCAACGGGCCCTGGACGATGCTGGTGATGGCGACCAT
CGGGTTCGCCGTGAACTTCTGGGCGTGGGCGCTGATCAGCCCGCTCGGCCCGCTGTTCCGGGACAACGGCCGGGTGGGCA
CGCTCAGCGAGTCCGACGTCGCGCTGATGGTCGCGGTGCCGGTCGTGGTCGGTTCACTGGGCCGGATCCTGGTGGGCGCG
CTGACGGACAAGTACGGCGGCCGGGTGATGTTCCCGTTGATCTCGGCGATCACGATCGTCCCGATCCTGTTTCTGGCGTT
CGTCGCCCTGGACTCCTACACGCTGATCCTGGTGGGTGGGTTCTTCCTCGGCATCGCCGGCACGGCGTTCGCCGTCGGCG
TGCCGTTCGTCAACGCGTGGTTCCCGCCGGAGAAGCGCGGGCTCGCCGTCGGCATCTTCGGCGCCGGCATGGGCGGCACG
GCGATCAGCGCGCTGACCACGGTCAAGCTCTACAAGAACATCGGTGACACCGCGCCGTTCTTGATCACCGCCACGGTGCT
CGCGGTGTACGCCGCGGCGGCCTGGCTGATCCTGCGCGACGCCCCCGGCCGCACCCCGCCGACCACCTCCCTGGCCGCGC
GCCTGGCGGCCACCTCGAAGCTGTCGATCACCTGGCAGGCCTGCATCCTCTACGCCGTCGCCTTCGGCGGGTACGTCGCG
TTCTCGGTCTACCTGCCGGCGTACCTCAAGACCGCCCACGGCCTCACCCCGGCAGACGCGTCCAACCGGATGGCGGGCTT
CGTGGTGATCGCTGTGCTGATGCGCCCGGTCGGCGGCTGGCTCTCCGACCGGTTCGGCGCGGTCCCCGTGCTCGCCTCCG
GCTACGCCGTGGTCGTGGTCTGCGCCGGCATCTCCGCCGGCGACCCGCCGCTGGACGGGATCGGCACCGTCGTGTTCCTC
GCGATGGCCGCGGCCCTCGGCTCCGGCAGCGGCGCGACCTTCGCCCTGATCGCCAAGGTCACCGAGCCCTCGCGGGTCGG
TGGCGTCACCGGCCTGGTCGGCGCCGCCGGCGGCCTGGGCGGCTTCGCGCCCCCGCTGGTGATGGGCTACGTCTACGGCC
GCACCGATTCCTACGCCATCGGTCTGTGGATGCTGTCGGTGACAGCAGCCCTGACCCTCGTGCTCACCCTGACCGTGGTT
CGACGGACCGCGGCCGAACACCGAACCGAGAGGATTCCTGCGTGA

Upstream 100 bases:

>100_bases
AGGGCCGCCGAGGGTGGTCCCTGGAGTTGGGGGTGTCCCGGTTGGCAACGTCAGTGATTTCGGACAAGGTGGTCGTGGAC
ACGATCTCGGGAGGTCGGGC

Downstream 100 bases:

>100_bases
CGATCGAGGGCACCTCCCAGCCCGGGCTCGACGGACCGCTCAGCGATGCGCTGGTGCGAAGTCGGCGGTACTTCACCCGG
GGGACCGTCTCGGAGGATCT

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MTVAEEGIVGSQRGLNGPWTMLVMATIGFAVNFWAWALISPLGPLFRDNGRVGTLSESDVALMVAVPVVVGSLGRILVGA
LTDKYGGRVMFPLISAITIVPILFLAFVALDSYTLILVGGFFLGIAGTAFAVGVPFVNAWFPPEKRGLAVGIFGAGMGGT
AISALTTVKLYKNIGDTAPFLITATVLAVYAAAAWLILRDAPGRTPPTTSLAARLAATSKLSITWQACILYAVAFGGYVA
FSVYLPAYLKTAHGLTPADASNRMAGFVVIAVLMRPVGGWLSDRFGAVPVLASGYAVVVVCAGISAGDPPLDGIGTVVFL
AMAAALGSGSGATFALIAKVTEPSRVGGVTGLVGAAGGLGGFAPPLVMGYVYGRTDSYAIGLWMLSVTAALTLVLTLTVV
RRTAAEHRTERIPA

Sequences:

>Translated_414_residues
MTVAEEGIVGSQRGLNGPWTMLVMATIGFAVNFWAWALISPLGPLFRDNGRVGTLSESDVALMVAVPVVVGSLGRILVGA
LTDKYGGRVMFPLISAITIVPILFLAFVALDSYTLILVGGFFLGIAGTAFAVGVPFVNAWFPPEKRGLAVGIFGAGMGGT
AISALTTVKLYKNIGDTAPFLITATVLAVYAAAAWLILRDAPGRTPPTTSLAARLAATSKLSITWQACILYAVAFGGYVA
FSVYLPAYLKTAHGLTPADASNRMAGFVVIAVLMRPVGGWLSDRFGAVPVLASGYAVVVVCAGISAGDPPLDGIGTVVFL
AMAAALGSGSGATFALIAKVTEPSRVGGVTGLVGAAGGLGGFAPPLVMGYVYGRTDSYAIGLWMLSVTAALTLVLTLTVV
RRTAAEHRTERIPA
>Mature_413_residues
TVAEEGIVGSQRGLNGPWTMLVMATIGFAVNFWAWALISPLGPLFRDNGRVGTLSESDVALMVAVPVVVGSLGRILVGAL
TDKYGGRVMFPLISAITIVPILFLAFVALDSYTLILVGGFFLGIAGTAFAVGVPFVNAWFPPEKRGLAVGIFGAGMGGTA
ISALTTVKLYKNIGDTAPFLITATVLAVYAAAAWLILRDAPGRTPPTTSLAARLAATSKLSITWQACILYAVAFGGYVAF
SVYLPAYLKTAHGLTPADASNRMAGFVVIAVLMRPVGGWLSDRFGAVPVLASGYAVVVVCAGISAGDPPLDGIGTVVFLA
MAAALGSGSGATFALIAKVTEPSRVGGVTGLVGAAGGLGGFAPPLVMGYVYGRTDSYAIGLWMLSVTAALTLVLTLTVVR
RTAAEHRTERIPA

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=371, Percent_Identity=24.2587601078167, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42504; Mature: 42372

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAEEGIVGSQRGLNGPWTMLVMATIGFAVNFWAWALISPLGPLFRDNGRVGTLSESDV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCE
ALMVAVPVVVGSLGRILVGALTDKYGGRVMFPLISAITIVPILFLAFVALDSYTLILVGG
EHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
FFLGIAGTAFAVGVPFVNAWFPPEKRGLAVGIFGAGMGGTAISALTTVKLYKNIGDTAPF
HHHHHHHHHHHHCCCHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHH
LITATVLAVYAAAAWLILRDAPGRTPPTTSLAARLAATSKLSITWQACILYAVAFGGYVA
HHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHH
FSVYLPAYLKTAHGLTPADASNRMAGFVVIAVLMRPVGGWLSDRFGAVPVLASGYAVVVV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHCCCEEEEE
CAGISAGDPPLDGIGTVVFLAMAAALGSGSGATFALIAKVTEPSRVGGVTGLVGAAGGLG
EECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHCCCHHHHHCCCCCC
GFAPPLVMGYVYGRTDSYAIGLWMLSVTAALTLVLTLTVVRRTAAEHRTERIPA
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TVAEEGIVGSQRGLNGPWTMLVMATIGFAVNFWAWALISPLGPLFRDNGRVGTLSESDV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCE
ALMVAVPVVVGSLGRILVGALTDKYGGRVMFPLISAITIVPILFLAFVALDSYTLILVGG
EHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
FFLGIAGTAFAVGVPFVNAWFPPEKRGLAVGIFGAGMGGTAISALTTVKLYKNIGDTAPF
HHHHHHHHHHHHCCCHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHH
LITATVLAVYAAAAWLILRDAPGRTPPTTSLAARLAATSKLSITWQACILYAVAFGGYVA
HHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHH
FSVYLPAYLKTAHGLTPADASNRMAGFVVIAVLMRPVGGWLSDRFGAVPVLASGYAVVVV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHCCCEEEEE
CAGISAGDPPLDGIGTVVFLAMAAALGSGSGATFALIAKVTEPSRVGGVTGLVGAAGGLG
EECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHCCCHHHHHCCCCCC
GFAPPLVMGYVYGRTDSYAIGLWMLSVTAALTLVLTLTVVRRTAAEHRTERIPA
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]